Performed on: 

September 7, 2021
Performed by:   

Ma. Verel P. Abella

Bryle Trixthane Navora

Marriane Geil M. Siobal

Submitted to:
Mr. Rico Antonio Mariano

Experiment #3
Title: Millon’s Test

I. Learning Objectives
 To show the presence of soluble protein through Millon’s Test.
 Determine how Millon’s Test works.

II. Materials
A. Chemicals/Reagents
 1% Tyrosine Solution
 Acified Mercuric Sulphate (Millon’s Reagent)
 1% Sodium Nitrite

B. Glasswares
 Test Tube
 Test Tube Holder
 Alcohol Lamp

III. Procedure
1. Take 1 ml test solution in dry test tube and 1 ml distilled water in
another tube as a control.
2. Add 1 ml of millon’s reagent and mix well. Then cool them under
tap water.
3. Boil them for a minute using the alcohol lamp.
4. Now add a few drops of 1% sodium nitrite. And heat them lightly.
Observe.
IV. Results and Observation

Figure 1: Result of Millon’s Test

The solution turned to brick red color when 1% of sodium nitrite was
added. While, the other one did not change in color.

V. Discussion
The brick red color that the solution turned into signifies that the
solution tests positive for Tyrosine and the Millon’s test is used
specifically to test for its presence.

VI. Conclusion
This test is used for particularly for Tyrosine, which is the only amino
acid that has a phenol group and hydroxyl group that is attached to a
benzene ring. In the test solution tyrosine’s phenol group was primarily
nitrated by the nitric acid. Then the nitrated mercury (I) and mercury (II)
ions which are the tyrosine complexes, form a red solution resulting to
the change of color in the experiment.

VII. References
https://www.youtube.com/watch?v=WaSCcl7SdaM
https://www.youtube.com/watch?v=YjdfZG0N2Uo