Performed on: 

September 17, 2021

Performed by:   

Ma. Verel P. Abella

Bryle Trixthane Navora

Marriane Geil M. Siobal

Submitted to:
Mr. Rico Antonio Mariano

Experiment #6
Title: Denaturation of Proteins Through Coagulation

I. Learning Objectives
 To Identify what is denaturation.
 Determine how denaturation works.

II. Materials
A. Chemicals/Reagents
 Alcohol
 Egg
 Salt

B. Glasswares
 Plastic Spoons
 Styrofoam Bowls

III. Procedure
1.) Prepare the materials.
2.) Separate the egg whites from the egg yolk. Set aside the yolk.
3.) Fill the Styrofoam bowls with egg whites and label them.
4.) Add 1 teaspoon of salt on one bowl and mix. Then add another
teaspoon and mix it again and observe.
5.) Then on the second bowl, add 1 teaspoon of isopropyl alcohol to the
egg white. Stir and observe.
IV. Results and Observation

Figure 1: Result of Denaturation through Coagulation with

Albumin and Salt.

Figure 2: Result of Denaturation through Coagulation with

Albumin and Alcohol.
 The first was one was Albumin and Salt. It is mixed and after a while,
white particles started to appear and this also happened with the second
experiment with the use of alcohol.
V. Discussion

The proteins that are present in the albumin started to react and
coagulate when mixed with salt and alcohol. As proteins deform or
unravel parts of structure that were hidden away get exposed and form
bonds with other protein molecules, so they coagulate (stick together)
and become insoluble in water. Salt is capable of disrupting bonds and
interaction in molecules thus leading to the change of protein structure
denaturing them. And for the alcohol egg white, the alcohol is a form of
natural solvent, these solvents are capable altering the protein structure
through the formation of ne hydrogen bonds that are found between the
alcohol and protein side chains.
VI. Conclusion

Protein denaturation is the change in the structure when a foreign

substance is added. We can conclude that when a protein undergoes
denaturation, it prevents the protein from doing the roles it usually does.
Denaturation of proteins is affected by temperature and pH level. It alters
the proteins’ biological, chemical, and physical properties through the
disruption of its structure. Denaturation of proteins are involved in a lot
of biological processes and also utilized to finding new drugs and how it
interacts with the proteins.
VII. References
https://www.youtube.com/watch?v=L_cXi7Z4bsw