MICRO SLIDES

1. STAPHYLOCOCCI
 gram stain : +ve ( violet coloured )
Spherical(cocci) in shape : 0.5-1
μm in diameter
Arranged in clusters (grape-like),
which is due to cell division in 3
planes and daughter cells being in
close proximity
 non-motile, non-sporing
2. STREPTOCOCCI
 gram stain +ve
 spherical in shape
(individual cell)
 arranged in pairs
and chains, which is
due to cell division
occurring in 1 plane
and daughter cells
failing to separate
completely
3. GONOCOCCI
 gram stain –ve (pink
coloured)
 arranged in pairs -
diplococci
 cocci are kidney in shape
 the line joining cocci in
pairs is perpendicular to
the long axis of cocci
 intra cellular ( eg. Acute
gonorrhea – urethral
discharge )
4. CLOSTRIDIUM TETANI
 gram stain +ve
 drum-stick appearance
 terminal and round spores-
present
5. MYCOBACTERIUM TUBERCULOSIS
 acid fast bacilli – ZN STAIN/AFB
STAIN
 slender, straight or slightly curved
bacilli with beaded appearance
 non-sporing, non-capsulated and
non-motile
 they're stained pink, the other cells
are blue ( blue background)
6. CORYNEBACTERIUM DIPHTHERIAE
 albert stain is used (others:
ponders and neisser’s stain)
 slender rods
 arranged in angles to each
other : L, V forms , Chinese
letter pattern / cuneiform
arrangement
 have polymetaphosphate/
metachromatic/ volutin/
babes earnest granules
(bluish purple) at the end
 green colour bacilli
Media :  pleomorphic, non-sporing,
non-capsulated
7. GRAM NEGATIVE BACTERIA

 gram –ve
 rod-shaped bacilli are pink
in colour
 arranged individually -
scattered
 eg. E.coli , proteus ,
klebsiella
8. CANDIDA SP.
 gram stain +ve
 oval budding yeast cells with
pseudohyphae
 opportunistic fungi
 causative agent for oral thrush and
vaginitis
9. CRYPTOCOCCUS
 negative staining : modified india
ink stain and nigrosin stain
demonstrate the capsule
 gram stain +ve round budding
yeast cells
 causative agent for meningitis
 can be grown on SDA (Sabouraud
dextrose agar)
10. PLASMODIUM FALCIPARUM
GAMETOCYTE

Thin and thick stain

11. TAENIA - SCOLEX
12. CERCARIA
13. REDIA
14. TICKS