LABORATORY REPORT

COURSE CODE BIO462 - BIOCHEMISTRY

GROUP AS257 1A (PART 2)

EXPERIMENT EXPERIMENT 1 : DILUTION AND MOLARITY

LECTURER’S NAME DR. HISYAM BIN ABDUL HAMID

STUDENT’S NAME SITI NURMAISARAH BINTI AHMAD SHUKRI (2022607976)

DATE OF 3 NOVEMBER 2022

EXPERIMENT

DATE OF 14 NOVEMBER 2022

SUBMISSION
i. How would you prepare :

a) 10 mℓ of a 1;10 dilution of a 1 M NaCl solution and what would the final

concentration of NaCl?
b) 80 mℓ of a 1:20 dilution of a 1 M NaCl solution
c) 50 mℓ of a 1:25 dilution of a 1M NaCl solution

(Based on the calculation shown)

In question a, we can prepare 10 mℓ of a 1;10 dilution of a 1 M NaCl solution by adding
1 mℓ stock solution with 9 mℓ of solvent and the final concentration of the solution would be
0.1M. In question b, we are able to prepare 80 mℓ of a 1:20 dilution of a 1 M NaCl solution by
adding 4 mℓ of stock solution with 76 mℓ of solvent. In question c, we are able to prepare 50 mℓ
of a 1:25 dilution of a 1M NaCl solution by adding 4 mℓ of stock solution with 76 mℓ of solvent.

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ii) How would you prepare exactly 6 mℓ of a 1/20 dilution (assume the concentration of
your starting solution ‘1’)

By referring to the calculation shown, we are able to prepare exactly 6 mℓ of a 1/20

dilution by adding 0.3 mℓ of the stock solution with 5.7 mℓ solvent.

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