The document compares and contrasts atomic absorption spectroscopy and UV-Vis spectroscopy. Both techniques analyze samples by measuring their absorption of specific wavelengths of light, but atomic absorption spectroscopy is used to analyze metals by passing light from a hollow-cathode lamp through a flame or graphite furnace containing the sample. UV-Vis spectroscopy analyzes colored and organic compounds using one of two light sources and a cuvette to hold liquid samples, with a monochromator selecting the wavelength that interacts with the analyte.
The document compares and contrasts atomic absorption spectroscopy and UV-Vis spectroscopy. Both techniques analyze samples by measuring their absorption of specific wavelengths of light, but atomic absorption spectroscopy is used to analyze metals by passing light from a hollow-cathode lamp through a flame or graphite furnace containing the sample. UV-Vis spectroscopy analyzes colored and organic compounds using one of two light sources and a cuvette to hold liquid samples, with a monochromator selecting the wavelength that interacts with the analyte.
The document compares and contrasts atomic absorption spectroscopy and UV-Vis spectroscopy. Both techniques analyze samples by measuring their absorption of specific wavelengths of light, but atomic absorption spectroscopy is used to analyze metals by passing light from a hollow-cathode lamp through a flame or graphite furnace containing the sample. UV-Vis spectroscopy analyzes colored and organic compounds using one of two light sources and a cuvette to hold liquid samples, with a monochromator selecting the wavelength that interacts with the analyte.
Nature of analytes tested - metals - colored compounds and
organic compounds
Light Source - hollow-cathode lamp - two types of lamps are
- provides the exact/specific used: a Deuterium lamp for wavelength for the metal ultraviolet measurements sample to absorb and a tungsten lamp for visible and near-infrared measurements. - provides an appropriate wavelength for the sample to absorb
Sample holder - Flame or Graphite furnace - Cuvette
Monochromator - Following the sample - The monochromator is
holder (flame or graphite placed near the sample. The furnace) is the wavelength selector allows a monochromator or specific wavelength to wavelength selector. It interact with the analyte, allows only a specific which is useful during wavelength of light to pass quantitative analysis where through. the maximum or wavelength arrive at the detector Other with the highest absorbance wavelengths of light that of the analyte should be may have been produced by used. the flame or the graphite furnace can thus be limited.