Document number: 841033_G_applic_Apo B, Date: 02/04, s.w. 6.

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Test definition APO B Calibr. parameters
Test type Photometric Test in use YES Calibration type NONLINEAR
Full name Apolipoprotein Low High Units Bias corr. in use NO
B
Online name Test limit 0.05 13.3 g/l Repeat time (d) 14
Result Unit g/l low( Ö ), high (!) Abs. error (mA) 5
Point/Calibrator Single
Number of decimals 2 Initial absorbance 0 2 A Rel. error (%) 2
Acceptance MANUAL
Acceptance AUTOMATIC Response limit
(mA)
Type of calibrator SERIES Min. *
Dilution 1 + 10 Dilution limit (!) 0.15 2.86 g/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
Sample type Apo Cal 0.78 55
Secondary dil. 1+ 2 50 Apo Cal 0.78 27
ýSerum ýPlasma ¨Urine Apo Cal 0.78 14
¨C.S.F ¨Other Correction factor 1 Apo Cal 0.78 7
More Apo Cal 0.78 4
Correction bias 0 Apo Cal 0.78 2

Test flow Reagent definition

Blank YES Normal cuvette
Antigen excess NO

Syringe BCR Stable

speed Id. on board
Sample & Name (days)
dilution
Incubation Incubation
Reagent Volume (µl) Raw S. with Time (sec.) Blank Reagent Time (sec.) λ 1 (nm)
APO BUFFER 18 WATER 200 APO B AS 420 340

Volume (µl) Disp. with Volume (µl) Resp. min (A) Volume (µl) λ 2 (nm) APO Normal 204 30
BUFFER
110 EXTRA 30 * 30 NONE

Disp. with Volume (µl) Special dil. Resp. max (A) Disp. with SPEDIL Normal 201 30
EXTRA 20 SPEDIL 0.2 WATER

Volume (µl) Dilution with Disp. with Volume (µl) APO B AS Normal 233 30
20 Special dil. EXTRA 10

Wash reagent Wash reagent Volume (µl) Wash reagent Meas. Type
NONE NONE 20 NONE NORMAL

Konelab™ APOLIPOPROTEIN B - code: 981663

Reagent: Liquids, ready-to-use.
Calibrator: Included in the kit. Add 1.00 ml distilled water and wait 30 min before use. Stability: 2 months at 2...8 °C after reconstitution.
! Values are lot dependent, refer to value sheet and General Information for calculation.
Ö determination limit.

Q.C. sample: LipoTrol 981653. Reconstitute with 3.00 ml of distilled water. Stable for 5 days at 2...8 °C.