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Fig. 6. Effects of LQ on Hippo signaling. LQ induced phosphorylation of the proteins

associated with Hippo pathway, LAST and YAP. (A) Western blot analyses were performed
PT

with the lysates of cells that had been treated with 100 µM LQ for the indicated time period.

β-actin was used to serve as a loading control. Protein levels were presented as relative band
CE

intensities to control group. Results were represented as the mean ± S.D. calculated from
AC

three independent trials. * P < 0.05. (B) The pictures of the fluorescence micrographs show

the induction of YAP resulting from the treatment of LQ in LX-2 cells. Cells were treated

with 100 µM LQ for 1hr and were stained Yap with FITC labelled secondary anti-rabbit

antibody. (C) Verteporfin inhibited HSCs activation by TGF-β1` (left). LQ and verteporfin

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