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Neome Pate) 9hevi H.

EnollNo 22o3ožO4Oo13

SUbiec+Nacne 9DA techanalogy cnd geme

heguletion

Depuatment M.Se Sem- TT micsobiology

LCollcge mme Siver oak univengity

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Plasmd Vecto3 Used ton aA tech nulenj

Definition
Pleomids cone matually
CcCuing cicles ,extuchonosamaL
double Stoconded DroA omoleculos Haut
hepliecte ctomomousy uoithin bcterical
cells,

S Pleesmics and bacteniophcigyes coe Enequenty

USed cS cloming vecto on 9roA hecomnbimu
ont technolagy

S o geanetc engianecnig pusposg, plusmids.

Ce Cntificiclly paepaned jm the Jab
Thelab-9owN plasomida,1ehich ce Used
An cigim
Seplicaticn cloaning site & selectom
msken
>Chonctenietice of plesm vectoi
- contains cm cgi site a oiqin of

hepliccatiom.
I lao Contoin 8elecive moDker Suca
cntibfotic gesistumce. blue ohite sxeeming.
Smcll in size C1.o to e5o kb)
-Coateins amultiple cloing site
tcsily isoluted hum the host cel.
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Types of plosmid vectons

e sesistamce plugmid
3) viaule nce plusmids
4)degaadctive plymids
&) Col plosmid

Yentility plasmids clso knonn as

-plsmmids Costoím taunsfen genes
thatloo genes to be toenSfennel
to cnothen thsoygh
Ehomone bectenic
Comiugetion

- These make-up the bpud ceitegoy of

Convgative plomids.

Buctenic
known cs
that he
. oL
positive
plasmid
C)Ln
ose
bocteria
othout ne C)
gcutie/e

e Resistonce Plesmids

- Hesisuonce o Plusam id contln

genes that help a bactenial cell.
defend Cqcumst emviaommem tol uctos
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- Some9esistunce Cam tonsfen.

plusonids
themselves thaough comívgtion.
Whem thishuppems,, c Gtauim of bucteniA
Cun become ge3istunt to antibíottcs.

Hhe bcteio Acconding to NPR, ONenUSe

Of antibichs to taecat othes ImFertons,
ke uigcy tuct nfectiosns, may
lecld to the prolifenction of doup
hegistunt stoind.

Viulence pleonmids ?
1ehen a yisulenee fmside
plusmid io
cl bocteniuam t tuns thtbacteniuam
of
puthogem hichiS cigent cn
dise cuse

Buctenic thet cciUSe diseciae cum be

eLsily Spneud dnd eplfected camong
affected imdividucus.

The bucteziusn Esahenichie coli CE-colD hu

sevenl visulemce plusmids

ScLmomelle enteicc i5 anothen bucterium

that contetns viaulence plsmida

4)Degtdative pleaomido
-
Deydctive plosmids help the host
buctenium to digest compovnd thet ahe
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not Commonly Toumd jm ntwse, su

Cg Cmphan, xyleme toluene, dnd
Salicylic cucid.

These plusmids Qomtein geme Fos

Specicl enymcs thut bneuk dous
SPecific Compounds,

Degudetive plesmids ce Comivsotive

Ca plcsmids
co) plegamid conteim genes that m
buteioci nS, lhich cLSe poteims taat
Ril)othen buteia Cnd husde fenà
the host buctenium

Bacteicocins Foumd
OF buctenic includimy
ce in muny types
Ecoli, lih
9e4s them Paom the ploymid CoEL

oceemples Plesamic vectcas cne

1ZpBR322
e pUc 19

PBR322-
T+ is One of the Fist
vectogs to be devloped by Boliven cLnd
Rodnigue n 1977.
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The mcme pBR322 demote, the Follouoíny

P- plesmid
B- Boliven
R- Rodhiquez
g22- DifFes.enticte i t hom the other plusmid
pnoduced in the Sme lbotoy E.3,
topBA325.pB R327 etc

- is 4368 bp in sireciaculca9,, double

Sanded clonfnq vecto. Mw:2,83 x 10 da

-4auies tu0 dontibiotc esisteLence genes

Ampicillín hesiseumee gene Ccung
etetr Cycline Sesistunce qene Ctet)
It hes simgle clecaucye Sites fan ÉcoRT Hind TE

EcoRI Himd

ZCORV
HOO0
S3BmtI
felmp C5) SeI
tet

pBR322

3000

200o
2295

PBRs22 geneticay engineesed From thace

is

dirfened ntrcly occwiy Plsmíds, R, Ro5&

pMB.
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pUC1g
Lac Selectlon plemid.

-Tn plc1g P- Plesmid

Uc- Vnivensity Of Ci fasnía

puc i5 aeleted to pBR322 huviny ginilas oigin

oF 9eplicatiam.

- pucg Smollen Ce686 bp

is y)than pBRa2:
but exst in omuch highen coPy mumben

-The cmp gene pesent ian this plcsmid, n

the sdentiicoton of tansfohmed cells

ÉoRI
Socl
Tms tpt
Sml
Bam
AmoR pUCig bt
Ce66bP) Scl
PstI
Sphl
\Hindst