Staphylococcus

R. A. Maghfirah

1. Name the gram-positive coccus that colonizes in the anterior of nares

S. Aureus

2. Name the gram-positive cocci that colonize in area where apocrine glands are
present
s. Haemolyticus and S. hominis
3

3. Name the gram-positive cocci that colonizes in area where sebaceous gland are
present
S. Capitis

4. What is the pigment that is causing Staphylococcus aureus colonies can have a
yellow or gold color
Carotenoid pigment
5

5. Which species that cannot cause endocarditis?

Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Staphylococcus lugdunensis
Staphylococcus haemolyticys
6

6. What is the type of Staphylococcus aureus capsule?

Polysaccharide capsule
7

7. Which of the following staphylococcal toxin that is not classified as

superantigen?
Exfoliative toxin A
Exfoliative toxin B
Enterotoxin A
Toxic shock syndrome toxin – 1
Enterotoxin C
2
8

8. What staphylococcal virulence factor that facilitates adherence to foreign

bodies?
Capsule
Slime layer
Protein A
Teichoic acid
Peptidoglycan
2
9

9. What staphylococcal virulence factor that stimulates production of endogenous

pyrogen and provide osmotic stability?
Capsule
Slime layer
Protein A
Teichoic acid
Peptidoglycan

5
10

10. What staphylococcal virulence factor that inhibit antibody-mediated clearance

by binding to IgG1, IgG2, and IgG4 Fc receptor?
Capsule
Slime layer
Protein A
Teichoic acid
Peptidoglycan

3
11

11. What staphylococcal virulence factor that bind to fibronectin?

Capsule
Slime layer
Protein A
Teichoic acid
Peptidoglycan

4
12

12. What staphylococcal toxin that split intracellular bridges in the stratum
granulosum
Exfoliative Toxin A dan B
13

13. What staphylococcal toxin that produce leakage or cellular destruction of

endothelial cell?
TSST-1
14

14. Which staphylococcal enzim that converts fibrinogen to fibrin?

Coagulase
Hyaluronidase
Fibrinolysin
Staphylokinase

15. Which staphylococcal cytotoxin that allows rapid efflux of K+ and influx of
Na+, Ca2+, and lead to osmotic swelling and cell lysis
Alpha toxin
Beta toxin
Delta toxin
Gamma toxin
Panton Valentine Leukocidin
1
16

16. Which staphylococcal cytotoxin that disrupting cellular membranes by act as

surfactant?
Alpha toxin
Beta toxin
Delta toxin
Gamma toxin
Panton Valentine Leukocidin

3
17

17. Which staphylococcal cytotoxin that is leukotoxic

PV leukocidin
18

18. Which staphylococcal cytotoxin that also called sphingomyelinase C?

Alpha toxin
Beta toxin
Delta toxin
Gamma toxin
Panton Valentine Leukocidin

19

19. Cell lysis by gamma and P-V Leukocidin toxin is mediated by a process, what is
it?
Pore formation
20

20. Gamma toxin and P-V leukocidin are bicomponent toxin that composed of 2
polypeptide chains, S and F proteins. Which one is NOT belong to S protein?
Hemolysin gamma A
Hemolysin gamma B
Hemolysin gamma C
LukS-PV
2
21

21. Gamma toxin and P-V leukocidin are bicomponent toxin that composed of 2
polypeptide chains, S and F proteins. Mention the component of F protein
(HlgB, LukF-PV
22

22. Either Exfoliative toxin A or B will split a cell adhesion structures. What is
it? (specific)
desmoglein-1
23

23. Which type of enterotoxin that causes staphylococcal pseudomembranous colitis?

Enterotoxin A
Enterotoxin B
Enterotoxin C
Enterotoxin D
2
24

24. Which enterotoxin that is resposible for nonmenstruation-associated Toxic

Shock Syndrome?
Enterotoxin A
Enterotoxin B
Enterotoxin D
Enterotoxin E
2
25

25. In neonates, Staphylococcus aureus colonize in what area (commonly)?

umbilical stump, skin, and perineal area of neonates
26

26. What is the localized form of Ritter’s disease?

Bullous impetigo
Folliculitis
Furuncle
Carbuncle
1
27

27. Which statement is NOT true about bullous impetigo?

The blisters are culture negative.
Nikolsky sign is not present
The erythema doesn’t extend beyond borders of blister
is highly communicable
1.
28

28. What is the most commonly contaminated foods in staphylococcal food poisoning
cases
salted meats
29

29. Certain strains of S. aureus can also cause enterocolitis. Mention 1 toxin that
can cause that disease
enterotoxin A and the bicomponent leukotoxin LukE/LukD
30

30. A particularly virulent form of toxic shock syndrome that is characterized by

large purpuric skin lesion, fever, hypotension, and disseminated intravascular
coagulation is...
Purpura fulminans
31

31. Which type of pneumonia that is commonly caused by MRSA?

Aspiration pneumonia
Hematogenous pneumonia
Necrotizing pneumonia
Emypema
3
32

32. Expression of virulence fators in staphylococci is under a complex control.

What is it?
Accessory gene regulator operon
33

33. Resistance to metichillin is mainly correlated with...

Its polysaccharide capsule and slime layer
mecA gene that make penicilin-binding protein has a high affinity fot related
penicilin
Production of penicililin-binding protein 2a
The ability of the bacteria to make complete hemolysis
Because it is coagulase-positive staphylococci

3
34

34. What is staphylococcal species most commonly associated with native valve
endocarditis?
Staphylococcus haemolyticus
Staphylococcus lugdunensis
Staphylococcus saprophyticus
Staphylococcus epidermidis
Staphylococcus capitis
2
35

35. Which disease below that is associated to Staphylococcus saprophyticus?

Urinary tract infection
Endocarditis
Surgical wound infection
Arthritis
1
36

36. Mention 2 selective agar for Staphylococcus aureus

chromogenic agar (where S. aureus colonies are a characteristic color) or
mannitol-salt agar
37

37. Mention 2 indicator for identify Staphylococcus aureus in simple biochemical

test.
positive reactions for coagulase, protein A, heat-stable nuclease, and mannitol
fermentation
38

38. What enzyme that cause Staphylococcus aureus resistance to penicillin?

penicillinase
39

39. What is the drug of choice for IV therapy in patients with localized skin and
soft-tissue infections
Vancomycin
Daptomycin
Tigecycline
Trimethoprim-sulfamethoxazole
Clindamycin

40. High level resistance of Vancomycin is mediated by...

VanA gene operon
41