Chlamydia & Chlamydophila (30 Q)#MURRAY p.

348-355
By : GE_AWAEH

1. The family Chlamydiaceae consists of two clinically important genera. What is

that?
Chlamydia and Chlamydophila
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2. Name three species responsible for human disease in family Chlamydiaceae.

Chlamydia trachomatis, Chlamydophila psittaci, and Chlamydophila pneumoniae

3. The Chlamydiaceae are obligate intracellular parasites that were once considered
as viruses; however, the organisms have the following properties of bacteria. Which
is uncorrect statement below?##(1) possess inner and outer membranes similar to
those of gram-positive bacteria#(2) contain both DNA & RNA#(3) possess prokaryotic
ribosomes#(4) synthesize their own proteins, nucleic acids, and lipids#(5) are
susceptible to numerous antibacterial antibiotics.
1. Gram negative bacteria
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4. Unlike other bacteria, the Chlamydiaceae have a unique developmental cycle. What
do they form?#
metabolically inactive infectious forms (elementary bodies [EBs]) and metabolically
active noninfectious forms (reticulate bodies [RBs]).

5. Which one is not disease that caused by Chlamydia trachomatis?#1. Adult

inclusion conjunctivitis#2. Neonatal conjunctivitis#3. Lymphogranuloma venereum #4.
Atherosclerosis #5. Urogenital infections
4. Atherosclerosis (Chlamydophila pneumoniae )
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6. Which one is the disease that caused by Chlamydophila pneumoniae ?#1. Adult
inclusion conjunctivitis#2. Neonatal conjunctivitis#3. Lymphogranuloma venereum #4.
Respiratory infections#5. Urogenital infections
4. Respiratory infections
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7. What is an important structural component of the outer membrane and is unique

for each species of Chlamydiaceae that found in cell wall?
The major outer membrane protein (MOMP)
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8. Variable regions in the gene encoding important and unique protein in cell wall
that found in C. trachomatis and are responsible for 18 serologic variants called
as
serovars
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9. This protein is a second highly conserved outer membrane protein, is shared by

all members of the family Chlamydiaceae. This cysteine-rich protein is responsible
for the extensive disulfide cross-links that provide the stability in the EBs. What
is the name of this protein?
OMP 2
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10. Characteristic:#- Host range: Primarily human pathogen #- Biovars : LGV and
trachoma #- Elementary body morphology : Round, narrow periplasmic space #-
Inclusion body morphology: Single round inclusion per cell #-Iodine-staining
glycogen in inclusions : Yes #-Susceptibility to sulfonamides: Yes##What spesific
species have this charateristic ?
Chlamydia trachomatis
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11. Characteristic:#- Host range: Primarily animal pathogen; occasionally infects

humans #- Biovars : Many #- Elementary body morphology : Round, narrow periplasmic
space #-Inclusion body morphology: Multiple variably sized inclusions per cell #-
Iodine-staining glycogen in inclusions : No#-Susceptibility to sulfonamides:
No##What spesific species have this charateristic ?
Chlamydophila psittaci
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12. Adhesin of Chlamydia trachomatis?##1. Fimbriae #2. P1, P2, P3 #3. Cell surface
lectin #4. Type 4 pili #5. Clumping factor A
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1. Neisseria gonorrhoeae
2. Treponema pallidum
4. Vibrio cholerae
5. Staphylococcus aureus
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13. Receptor of Chlamydia trachomatis?##1. GD1 ganglioside #2. Fibronectin #3.

Sialic acid #4. Fibrinogen #5. N-Acetylglucosamine
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1. Neisseria gonorrhoeae
2. Treponema pallidum
3. Mycoplasma pneumoniae
4. Staphylococcus aureus

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14. The range of cells that C. trachomatis can infect is limited. Receptors for EBs
are primarily restricted . What type of epithelial cells that can infected by this
bacteria which are found on the mucous membranes?
nonciliated columnar, cuboidal, and transitional epithelial cells
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15. The LGV serovars are more invasive than the other serovars. WHY?#
because they replicate in mononuclear phagocytes
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16. Which one is not serovar of Trachoma?#1. A#2. B#3. C #4. D#

4. D (Urogenital tract disease)
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17. Which one is not serovar of Lymphogranuloma venereum?#1. L1a#2. L2 #3. L2a#4.
L2b #5. L3
1. L1a (GAK ADA)
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18. What syndrome that believe is initiated by genital infection with C.

trachomatis?
Reiter syndrome
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19.What is the test of choice for the diagnosis of chlamydia infections (generally
reported to be 90% to 98% sensitive and very specific)?
Nucleic acid amplification tests (NAATs)
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20. Which statement not true?#1. Patients with LGV is treated with doxycycline for
21 days. #2. Erythromycin is not recommended for pregnant women#3. Ocular and
genital infections in adults treated with azithromycin for 7 days. #4. Newborn
pneumonia should be treated with erythromycin for 10 to 14 days.
2
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21. Chlamydophila pneumoniae has been associated with inflammatory plaques in blood
vessels. What is the disease?
Atherosclerosis
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22. What are characteristic inclusion body morphology of Chamydophila pneumoniae ?

Multiple uniform inclusions per cell
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23. What are characteristic elementary body morphology of Chamydophila pneumoniae ?

Pear-shaped, large periplasmic space
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24. What is biovar of Chlamydophila pneumoniae?

TWAR
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25. Diagnosis of Chlamydophila pneumoniae infections is difficult. The organisms do

not grow in the cell lines used for the isolation of C. trachomatis. What is cell
line that will grow Chlamydophila pneumoniae ?
HEp-2 cell line
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26. Which is antibiotic that not recommended for treatment of Chlamydophila

pneumoniae infections? #1. erythromycin#2. azithromycin#3. levofloxacin#4.
doxycycline#5. ciprofloxacin
5. ciprofloxacin
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27. What is the name of disease that caused by Chlamydophila psittaci ?

psittacosis (parrot fever)
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28. How can Chlamydophila psittaci transmitted to human?

through the inhalation of dried excrement, urine, or respiratory secretions from
psittacine birds (e.g., parrots, parakeets, macaws, cockatiels).
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29. What is antibiotic can treat Psittacosis?

doxycycline or macrolides
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30. Psittacosis can be prevented only through the control of infections in domestic
and imported pet birds. Such control can be achieved by treating birds with what?
chlortetracycline hydrochloride for 45 days.
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