REOVIRIDAE (40 Q)#CHAPTER 55 #MURRAY P.

503#
By: GE_AWAEH

1. The name rotavirus is derived from the Latin word ROTA, What is the meaning of
ROTA?#1. GASTER#2. WHEEL#3. OVAL#4. DIARRHEA#
Wheel, which refers to the virion’s appearance in negative-stained electron
microscope
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2. Describe the morphology and genome of reoviruses!#

Nonenveloped icosahedral capsid, segmented dsRNA. Reoviruses are the
only medically significant dsRNA viruses.

3. How is rotavirus transmitted?

Via fecal-oral route.

4. Name the members that consist of family Reoviridae!

the orthoreoviruses, rotaviruses, orbiviruses, and coltiviruses.#
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5. The orbiviruses and coltiviruses are spread by _______ and are arboviruses.#
Arthropods

6. The Reoviridae are nonenveloped viruses with double-layered protein capsids

containing _____ in number of segments of the double-stranded ribonucleic acid
(dsRNA) genomes. #
10-12 segments

7. Rotaviruses cause _________ (specific), a very common disease.#

Human infantile gastroenteritis

8. Proteolytic cleavage of the outer capsid (as occurs in the gastro-intestinal

tract) activates the rotavirus for infection and produces
_______________________________.#
An intermediate/infectious subviral particle (ISVP).

9. There are four Rotavirus gene product that have funtion as RNA binding. Mention
2 of this protein?#
NSP1 (NS53)
NSP3 (NS34)
NSP2 (NS35)
NSP5 (NS26)

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10. The genera are divided into two subfamilies: ________ contains viruses with
large spikes at the 12 vertices on the particle (e.g., Orthoreovirus), whereas
members of the _________ appear more smooth, lacking the large surface projections
(e.g., Rotavirus).#
Spinareovirinae, Sedoreovirinae.

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11. How is Coltivirus transmitted?#

Coltivirus is carried in rodents and transmitted via a wood-tick vector. The
disease is endemic to the Rocky Mountains and infects hikers and campers.

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12. What virus showed in above?

Rotavirus

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13. BLUE, RED or YELLOW that have function transcriptase component?

RED VP 2 : transciptase component

BLUE (VP1/VP3): polymerase and mRNA capping

YELLOW VP 4 : VAP

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14. What is the enzyme that responsible for synthesizing negative-sense strands to
form the double-stranded genome segments?#1. Viral transcriptase#2. Viral
replicase#3. Viral polymerase#4. Viral translatase#
2. Viral replicase

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15. This virus is the most important cause of gastroenteritis in infants and young
children. It causes infections that are often severe and may be life threatening,
especially in infants.#1) Echovirus#2) Norwalk virus#3) Rotavirus, group A#4)
Orbivirus#5) Parvovirus#
3
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16. An outbreak of epidemic gastroenteritis occurred at a wooded summer camp 24

hours after a party for visiting families. Some of the visiting parents also became
ill. Samples taken 2 weeks later from the well that was the source of drinking
water at the camp were negative for fecal coliforms. The most likely source of the
outbreak was:#1) Mosquitoes or ticks, present in high numbers in the area#2)
Contaminated food served at the party#3) A nearby stream used for fishing#4) A
visiting parent who was developing pneumonia#5) The swimming pool#
2
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17. This viral gastroenteritis agent has a segmented, doublestranded RNA genome and
a double-shelled capsid. It is a member of which virus family?#1) Adenoviridae#2)
Astroviridae#3) Caliciviridae#4) Reoviridae#5) Coronaviridae#
4
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18. Rotaviruses and astroviruses share a number of characteristics. Which of the

following is not shared?#1) Multiple serotypes exist#2) Can cause gastroenteritis
in infants and children#3) Can cause gastroenteritis in elderly institutionalized
patients#4) Live vaccine available#5) Fecal–oral route of transmission#
4

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19. VP7 and NSP4 are synthesized as glycoproteins . Where their will be expressed ?
#
In endoplasmic reticulum
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20. What is viral attachment protein of Rotavirus?#1. VP 1#2. VP 2#3. VP 4#4. VP 6#

3. VP4
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21. BLUE, RED or YELLOW that have functions polymerase and mRNA capping?
BLUE (VP1/VP3)

RED VP 2 : transciptase component

YELLOW VP 4 : VAP
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22. The σ1/VP4 protein at the vertices of the ISVP (Infectious/intermediate

subviral particle) binds to ____________ on epithelial and other cells.#
Sialic acid–containing glycoproteins

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23. What cell attacked by Rota virus?#

Enterocytes, intestinal villi

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24. What is a viral enterotoxin and induces secretion by triggering a signal

transduction pathway?#
NSP4

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25. Mention laboratory diagnosis facilities can be used for diagnosing Rotavirus
infection!
PCR for nucleic acid in stool, enzyme immuno assays in stoll (EIA (more
sensitive)/IEM), and antibody titer or ELISA.

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26. What treatment and control for gastroenteritis?#

Treatment of gastroenteritis is supportive to correct the loss of water and
electrolytes that may lead to dehydration, acidosis shock, and death.
Management consists of replacement of fluids and restoration of electrolyte balance
either intravenously or orally as feasible.
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27. What is the viruses that cause Colorado tick fever and related human disease?#
Coltiviruses

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28. What is the virus that mainly cause disease in animals, including blue tongue
disease of sheep, African horse sickness, and epizootic hemorrhagic disease of
deer?#1) Echovirus#2) Norwalk virus#3) Rotavirus, group A#4) Orbivirus#5)
Parvovirus#
4. Orbiviruses

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29.What is the acute disease characterized by fever, headache, and severe myalgia,
was originally described in the 19th century and is now believed to be one of the
most common tick-borne viral diseases in the United States?#
Colorado tick fever

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30. What treatment for Colorado tick fever?#

No specific treatment is available for Colorado tick fever. The disease is
generally self-limited, indicating that supportive care is sufficient.
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31. Neuronal infection by coltivirus can lead to meningitis and encephalitis.

(T/F)?#
TRUE
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32. Serious hemorrhagic disease can result from infection of vascular endothelial
and vascular smooth muscle cells and pericytes, thereby weakening capillary
structure. The weakness leads to leakage, hemorrhage, and potentially hypoten-sion
and shock. These are pathogenesis of coltiviruses. (T/F)?#
TRUE
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33. Orthoreovirus can not cause biliary atresia. (T/F) ?

FALSE
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34. Reovirus are unstable in detergents, over wide pH and temperature ranges, and
in airborne aerosols. (T/F)?#
FALSE
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35. Rotaviruses have a double-layered structure. (T/F) ?#

False, it must be TRIPLE-LAYERED.

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36. Rotavirus has same characteristic with the other Reoviridae, so pseudoenvelope
does not exist on Rota virus morphogenesis. (T/F)?#
False

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37. Group B rotaviruses have been implicated in large outbreaks of severe

gastroenteritis in adults in China. (T/F)?
TRUE
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38. Additional receptors include the β-adrenergic receptor for reovirus and
integrin molecules for rotavirus. (T/F)?#
TRUE
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39. The complete virion is then partially digested in the gastrointestinal tract
and activated by protease cleavage and loss of the external capsid proteins
(σ3/VP7) and the VP4 of rotavirus also promotes penetration of the virion into the
cell. (T/F)#
TRUE
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40. The pathogenesis of Coltivirus infection : Infection leads to viremia and

multiorgan lesions causing fever, headache, retro-orbital pain, and myalgia.
Pancytopenia may result from bone marrow involvement. (T/F)#
TRUE
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