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|Page No.

Date.

AlM 0£ THE
EXPER*MENIi
pzepanalion
o Eschenitbia coil:L6CLeia Bertani) rnedium and aisineg

REQUIRLMCN1S
LB medium equiru l0g Nac
Csodiuy chloide), log.
yptone, 5g daicc yeaut, cleioniuq Water
onica) blcuk mecLsuing 6laks cotto) plugsHaminar
Glou -chambex adoclove ipcubatoo etc:

PROCEDURE
is lhe
sanclazd nedium ocaou) badzia epaially
Escberichia Coil luraia- Bealan meaiug.
macium is alsa
as tuuna bopth Lenpox bpth

a. Take Q5m dercnitd oatex and ada tog sodíium


clonde lay toypione and sgdid yeasf fix uet

3 ndjut
3.

Adjust taing deicniud


weters.
5- sdeaiire 1he Sclutio) atter fcaing incenica
blanka end iher luggin ihem Coith cotten

Teacher's Signature
tatto Shaxinq
1acc
No.
Page eie
Date. in
inautoclare biek tontinicus Teac
Signa
1rcs6ex
obine,
oity
the
to
flaxk oh
ewtwxe
oud
coil: gi't
carned tbe ai
Glou
oftscbenibia Lnon-
ansoxbert)
plugs
4he
moth
socubate
3:ovexnighf
is lcrninaz
steaiitalon
Raising
Ving Sealec

6.
Expt.
No.
OXrORD
(b) double-stranded
origin unwinds
thereplication atDNA
MolBieculoloagyr
REPLICATION:
THETA
(a)
E.coli

replicationorigin of
fork
bubble replication
replication
replicationorigin of

a templates
fork bubble
usually
replication
havingforms, stranded
for single
new..producing
at DNA. he t
each synthesis
A
end.replication
of
2. replication
bubble
synthesized
newlyDNA
replication
fork
around
circletheforksThe
proceed

CONCLUSION:
replication

eventually
molecules
produced.
are
are
two
the
circular
products two
circular
DNA
of DNA
molecules. theta
135
|Page No.
Date.

SDENTIyINLI CHDRACTERI

J Thcia 3zpleattor Rsa


ia type f reptikceton umnory
e5hei chi a. coil and othex ogganis pusesáng
itex DNA
QDouble Haanda &nA tunoinds LTepitatig coigig
Thus traning

epicatiorn dlenatcaed
bublale The DNA fnounNcinds to

yhapt
4 The fusks pnoueancune the cile
eisulcUs NA molecelesare
euentuculy

Sdenhiti catog: this mi


Sue t0presence of cehore chcuractex
jhotogauph identisied Theta Repicaten
olecular
B/ology
pneumoniae S.
strain
pathogenic of
heat-kiled
mice
live

Coat
polysaccharide
Griffith's
pneumoniae S. of live
strain
pathogenic
micedie

experiment

strain live
S. of
nonpathogenic
pneumoniae of
micelive
transformation

contains
thogenic
strains livemice
S. of
oniae nonpatho
strains
pneumoniaegenic
S. oflive
andkilled
pathogenicmixture
heat of
die;
their
blood

141
Expt.
No.

viulert 5. Arimlent4.
darklepad il e olevzlapano pnemoni viulenf 3 dentiying
40
o cupsude. o00 demo
a b afoate
and
aviTulent Stumied
nouseono Sl.
and s11 thaxades:
then RlLS I1R thct
cied coniaull C facu
pndtmCOLCUS upswct &ND
íngectee Hacçry
RI Ld is
into Mousemice hy
44pe
into
t tapsdcar) ea)
qenetícmetenc.
pneumo
neumoCOCCLA
lure
er's
MouseNonto the
ano
dicnt) beteia pneuno
and mouse ual visul CocUs ee
hect coccus dlucedepee
were
Genete ent
o avilent. bateia Date.PageNo.
betetkilled diseae The
hRet
battcasq metaia)
kuomalBar
the
Sue
Jdeniticoaioy pheumco
Hcwevex
batexia.CLM)
t0
prseacne
hQase

QNA
t
ahaveHorcnstoanclion
charades
matesial
qenstic a
Werelso

Jhis
|Date.PageNo.

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