ALP

Fosfatasa alcalina
p-Nitrofenilfosfato. Cinético. Líquido. DGKC

PREPARACIÓN Y ESTABILIDAD
Ref. 41240 o 41242. Ambos reactivos se encuentran listos para el uso.
A_15 / A_25
Son estables hasta la fecha de caducidad indicada en el envase.

CALIBRACIÓN
Utilizar un suero de calibración adecuado (CFAS Ref. 1002011)

CONDICIONES OPERATORIAS
Introducir en la memoria del ordenador los siguientes parámetros:

ALP A_15 A_25

1.GENERAL:
REACTTION TYPE INCREASE INCREASE
SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
UNITS: U/L U/L
# DE REPLICTES: 1 1
# DECIMALS: 1 1
ASSAY METHOD: KINETIC KINETIC

2.PROCEDURE:
BOCHROMATIC READING
1 ST WAVELENGTH: 405 405
2 ND WAVELENGTH:

3.VOLUMES:
SAMPLE VOL: 5 ul 5 ul
REAG1 VOL: 240 ul 240 ul
REAG2 VOL: 60 ul 60 ul
CLEAN: 1.2 1.2

3.TIME:
READING 1: 252 SEC / C12 255 SEC / C18
READING 2: 432 SEC / C21 435 SEC / C31
REAGENT 2: 192 SEC / C9 195 SEC / C14

4.CALIBRATION.
CALIBRATOR. SPECIFIC SPECIFIC
CAL NUMB: 1 1
CAL REPLICATES: 3 3
BLK REPLICATES: 3 3
MULTIPOINT CAL:

5.CONTROLS:
# CONTRLS: 1 1
REJECTION CRITERIA 3 DS 3 DS
CONTROL TYPE: MULTIPLE. MULTIPLE
CALCUL METHOD: MANUAL MANUAL
#BATCH:
BATCH:
NAME:
LIMITS:

6.OPTIONS
BLK ABS. LIMIT YES YES
VALUE:

Ed. 2018

SPINREACT, S.A.U. Ctra. Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
 ALP

Alkaline phosphatase
p-Nitrophenylphosphate. Kinetic. Liquid. DGKC

PREPARATION AND STABILITY A_15 / A_25

Ref. 41240 or 41242. Both reagents are ready to use.
Stable up to the expiration date stated on the label.

CALIBRATION
Use a human serum calibrator (CFAS Ref. 1002011).

OPERATORY CONDITIONS
Introduce the following data in the computer:

ALP A_15 A_25

1.GENERAL:
REACTTION TYPE INCREASE INCREASE
SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
UNITS: U/L U/L
# DE REPLICTES: 1 1
# DECIMALS: 1 1
ASSAY METHOD: KINETIC KINETIC

2.PROCEDURE:
BOCHROMATIC READING
1 ST WAVELENGTH: 405 405
2 ND WAVELENGTH:

3.VOLUMES:
SAMPLE VOL: 5 ul 5 ul
REAG1 VOL: 240 ul 240 ul
REAG2 VOL: 60 ul 60 ul
CLEAN: 1.2 1.2

3.TIME:
READING 1: 252 SEC / C12 255 SEC / C18
READING 2: 432 SEC / C21 435 SEC / C31
REAGENT 2: 192 SEC / C9 195 SEC / C14

4.CALIBRATION.
CALIBRATOR. SPECIFIC SPECIFIC
CAL NUMB: 1 1
CAL REPLICATES: 3 3
BLK REPLICATES: 3 3
MULTIPOINT CAL:

5.CONTROLS:
# CONTRLS: 1 1
REJECTION CRITERIA 3 DS 3 DS
CONTROL TYPE: MULTIPLE. MULTIPLE
CALCUL METHOD: MANUAL MANUAL
#BATCH:
BATCH:
NAME:
LIMITS:

6.OPTIONS
BLK ABS. LIMIT YES YES
VALUE:

Ed. 2018

SPINREACT,S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN

Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com