ALP

Fosfatasa alcalina
p-Nitrofenilfosfato. Cinético. AMP buffer (IFCC)
PREPARACIÓN Y ESTABILIDAD
Ref. 41245 o 41246. Ambos reactivos se encuentran listos para el uso. Son estables hasta A_15 / A_25
la fecha de caducidad indicada en el envase.

CALIBRACIÓN
Utilizar un suero de calibración adecuado (CFAS Ref. 1002011).

CONDICIONES OPERATORIAS
Introducir en la memoria del ordenador los siguientes parámetros:

ALP A_15 A_25

1.GENERAL:
REACTTION TYPE INCREASE INCREASE
SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
UNITS: U/L U/L
# DE REPLICTES: 1 1
# DECIMALS: 1 1
ASSAY METHOD: KINETIC KINETIC

2.PROCEDURE:
BOCHROMATIC READING
1 ST WAVELENGTH: 405 405
2 ND WAVELENGTH:

3.VOLUMES:
SAMPLE VOL: 6 µL 6 µL
REAG1 VOL: 240 µL 240 µL
REAG2 VOL: 60 µL 60 µL
CLEAN: 1.2 1.2

3.TIME:
READING 1: 252 SEC / C12 255 SEC / C18
READING 2: 432 SEC / C21 435 SEC / C31
REAGENT 2: 192 SEC / C9 195 SEC / C14

4.CALIBRATION.
CALIBRATOR. SPECIFIC SPECIFIC
CAL NUMB: 1 1
CAL REPLICATES: 3 3
BLK REPLICATES: 3 3
MULTIPOINT CAL:

5.CONTROLS:
# CONTRLS: 1 1
REJECTION CRITERIA 3 DS 3 DS
CONTROL TYPE: MULTIPLE. MULTIPLE
CALCUL METHOD: MANUAL MANUAL
#BATCH:
BATCH:
NAME:
LIMITS:

6.OPTIONS
BLK ABS. LIMIT YES YES
VALUE:

REV.01 Ed. 2018

SPINREACT, S.A.U. Ctra. Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
 ALP

Alkaline phosphatase
p-Nitrophenylphosphate. Kinetic. AMP buffer (IFCC)
PREPARATION AND STABILITY
Ref. 41245 o 41246. Both reagents are ready to use. Stable up to the expiration date
A_15 / A_25
stated on the label.

CALIBRATION
Use a human serum calibrator (CFAS Ref. 1002011).

OPERATORY CONDITIONS
Introduce the following data in the computer:

ALP A_15 A_25

1.GENERAL:
REACTTION TYPE INCREASE INCREASE
SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
UNITS: U/L U/L
# DE REPLICTES: 1 1
# DECIMALS: 1 1
ASSAY METHOD: KINETIC KINETIC

2.PROCEDURE:
BOCHROMATIC READING
1 ST WAVELENGTH: 405 405
2 ND WAVELENGTH:

3.VOLUMES:
SAMPLE VOL: 6 µL 6 µL
REAG1 VOL: 240 µL 240 µL
REAG2 VOL: 60 µL 60 µL
CLEAN: 1.2 1.2

3.TIME:
READING 1: 252 SEC / C12 255 SEC / C18
READING 2: 432 SEC / C21 435 SEC / C31
REAGENT 2: 192 SEC / C9 195 SEC / C14

4.CALIBRATION.
CALIBRATOR. SPECIFIC SPECIFIC
CAL NUMB: 1 1
CAL REPLICATES: 3 3
BLK REPLICATES: 3 3
MULTIPOINT CAL:

5.CONTROLS:
# CONTRLS: 1 1
REJECTION CRITERIA 3 DS 3 DS
CONTROL TYPE: MULTIPLE. MULTIPLE
CALCUL METHOD: MANUAL MANUAL
#BATCH:
BATCH:
NAME:
LIMITS:

6.OPTIONS
BLK ABS. LIMIT YES YES
VALUE:

REV.01 Ed. 2018

SPINREACT,S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN

Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com