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 Ignacio Rojas
Francisco Ortuño (Eds.)
LNBI 10209

Bioinformatics and
Biomedical Engineering
5th International Work-Conference, IWBBIO 2017
Granada, Spain, April 26–28, 2017
Proceedings, Part II

123
Lecture Notes in Bioinformatics 10209

Subseries of Lecture Notes in Computer Science

LNBI Series Editors

Sorin Istrail
Brown University, Providence, RI, USA
Pavel Pevzner
University of California, San Diego, CA, USA
Michael Waterman
University of Southern California, Los Angeles, CA, USA

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Technical University of Denmark, Kongens Lyngby, Denmark
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IITP, Research and Training Center on Bioinformatics, Moscow, Russia
Thomas Lengauer
Max Planck Institute for Informatics, Saarbrücken, Germany
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University of Tokyo, Tokyo, Japan
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Max Planck Institute of Molecular Cell Biology and Genetics, Dresden,
Germany
Marie-France Sagot
Université Lyon 1, Villeurbanne, France
David Sankoff
University of Ottawa, Ottawa, Canada
Ron Shamir
Tel Aviv University, Ramat Aviv, Tel Aviv, Israel
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Max Planck Institute for Molecular Genetics, Berlin, Germany
W. Eric Wong
University of Texas at Dallas, Richardson, TX, USA
More information about this series at http://www.springer.com/series/5381
Ignacio Rojas Francisco Ortuño (Eds.)
•

Bioinformatics and
Biomedical Engineering
5th International Work-Conference, IWBBIO 2017
Granada, Spain, April 26–28, 2017
Proceedings, Part II

123
Editors
Ignacio Rojas Francisco Ortuño
Universidad de Granada Universidad de Granada
Granada Granada
Spain Spain

ISSN 0302-9743 ISSN 1611-3349 (electronic)

Lecture Notes in Bioinformatics
ISBN 978-3-319-56153-0 ISBN 978-3-319-56154-7 (eBook)
DOI 10.1007/978-3-319-56154-7

Library of Congress Control Number: 2017935843

LNCS Sublibrary: SL8 – Bioinformatics

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 Preface

We are proud to present the set of final accepted full papers for the third edition of the
IWBBIO conference “International Work-Conference on Bioinformatics and
Biomedical Engineering” held in Granada (Spain) during April 26–28, 2017.
The IWBBIO 2017 (International Work-Conference on Bioinformatics and
Biomedical Engineering) seeks to provide a discussion forum for scientists, engineers,
educators, and students about the latest ideas and realizations in the foundations,
theory, models, and applications for interdisciplinary and multidisciplinary research
encompassing the disciplines of computer science, mathematics, statistics, biology,
bioinformatics, and biomedicine.
The aim of IWBBIO is to create a friendly environment that could lead to the
establishment or strengthening of scientific collaborations and exchanges among
attendees, and, therefore, IWBBIO 2017 solicited high-quality original research papers
(including significant work in progress) on any aspect of bioinformatics, biomedicine,
and biomedical engineering.
The following topics were especially encouraged: new computational techniques
and methods in machine learning; data mining; text analysis; pattern recognition; data
integration; genomics and evolution; next-generation sequencing data; protein and
RNA structure; protein function and proteomics; medical informatics and translational
bioinformatics; computational systems biology; modelling and simulation and their
application in the life science domain, biomedicine, and biomedical engineering. The
list of topics in the successive call for papers also evolved, resulting in the following
list for the present edition:
1. Computational proteomics. Analysis of protein–protein interactions. Protein
structure modelling. Analysis of protein functionality. Quantitative proteomics and
PTMs. Clinical proteomics. Protein annotation. Data mining in proteomics.
2. Next-generation sequencing and sequence analysis. De novo sequencing,
re-sequencing, and assembly. Expression estimation. Alternative splicing discovery.
Pathway analysis. Chip-seq and RNA-Seq analysis. Metagenomics. SNPs
prediction.
3. High performance in bioinformatics. Parallelization for biomedical analysis.
Biomedical and biological databases. Data mining and biological text processing.
Large-scale biomedical data integration. Biological and medical ontologies. Novel
architecture and technologies (GPU, P2P, Grid etc.) for bioinformatics.
4. Biomedicine. Biomedical Computing. Personalized medicine. Nanomedicine.
Medical education. Collaborative medicine. Biomedical signal analysis. Biomedi-
cine in industry and society. Electrotherapy and radiotherapy.
5. Biomedical engineering. Computer-assisted surgery. Therapeutic engineering.
Interactive 3D modelling. Clinical engineering. Telemedicine. Biosensors and data
acquisition. Intelligent instrumentation. Patient monitoring. Biomedical robotics.
Bio-nanotechnology. Genetic engineering.
VI Preface

6. Computational systems for modelling biological processes. Inference of bio-

logical networks. Machine learning in bioinformatics. Classification for biomedical
data. Microarray data analysis. Simulation and visualization of biological systems.
Molecular evolution and phylogenetic modelling.
7. Health care and diseases. Computational support for clinical decisions. Image
visualization and signal analysis. Disease control and diagnosis. Genome-phenome
analysis. Biomarker identification. Drug design. Computational immunology.
8. E-health. E-health technology and devices. E-health information processing.
Telemedicine/e-health application and services. Medical image processing. Video
techniques for medical images. Integration of classical medicine and e-health.
After a careful peer-review and evaluation process (each submission was reviewed
by at least two, and on average 3.2, Program Committee members or additional
reviewers), 120 papers were accepted for oral, poster, or virtual presentation, according
to the recommendations of the reviewers and the authors’ preferences, and to be
included in the LNBI proceedings.
During IWBBIO 2017, several Special Sessions were carried out. Special Sessions
are a very useful tool to complement the regular program with new and emerging topics
of particular interest for the participating community. Special Sessions that emphasize
multi-disciplinary and transversal aspects as well as cutting-edge topics were especially
encouraged and welcomed, and in IWBBIO 2017 they were the following:
– SS1: Advances in Computational Intelligence for Critical Care
Decision-making in health care in clinical environments is often made on the basis
of multiple parameters and in the context of patient presentation, which includes the
setting and the specific conditions related to the reason for admission and the
procedures involved. The data used in clinical decision-making may originate from
manifold sources and at multiple scales: devices in and around the patient, labo-
ratory, blood tests, omics analyses, medical images, and ancillary information
available both prior to and during the hospitalization.
Arguably, one of the most data-dependent clinical environments is the intensive
care unit (ICU). The ICU environment cares for acutely ill patients. Many patients
within ICU environments, and particularly surgical intensive care units (SICU), are
technologically dependent on the life-sustaining devices that surround them. Some
of these patients are indeed dependent for their very survival on technologies such
as infusion pumps, mechanical ventilators, catheters and so on. Beyond treatment,
assessment of prognosis in critical care and patient stratification combining different
data sources are extremely important in a patient-centric environment.
With the advent and quick uptake of omics technologies in critical care, the use of
data-based approaches for assistance in diagnosis and prognosis becomes para-
mount. New approaches to data analysis are thus required, and some of the most
interesting ones currently stem from the fields of computational intelligence
(CI) and machine learning (ML). This session is particularly interested in the
proposal of novel CI and ML approaches and in the discussion of the challenges for
the application of the existing ones to problems in critical care.
Topics that are of interest to this session include (but are not necessarily limited to):
 Preface VII

• Novel applications of existing CI and ML and advanced statistical methods in

critical care
• Novel CI and ML techniques for critical care
• CI and ML-based methods to improve model interpretability in a critical care
context, including data/model visualization techniques
• Novel CI and ML techniques for dealing with non-structured and heterogeneous
data formats in critical care
Organizers: Dr. Alfredo Vellido, PhD, Department of Computer Science, Univer-
sitat Politécnica de Catalunya, BarcelonaTECH (UPC), Barcelona (Spain). Dr.
Vicent Ribas, eHealth Department, EURECAT Technology Centre of Catalonia,
Barcelona, Barcelona (Spain).
– SS2: Time-Lapse Experiments and Multivariate Biostatistics
Biological samples are evolving in time, phases, periods, behavior. To be able to
understand the dynamics, we need to perform time lapse experiments. Today’s
technique and measurement devices allow us to monitor numerous parameters in
semi-controled environments during the experiment. The increase of measured data
is enormous. The interpretation requires both qualitative and quantitative analysis.
There are useful methods of biostatistics, multivariate data analysis, and artificial
intelligence, namely, neural networks, genetic algorithms, and agent-based mod-
eling, respectively.
In this special section we will provide a discussion on broad examples from
time-lapse experimental design through information and data acquisition, using
methods of bioinformatics, biophysics, biostatistics, and artificial inteligence. The
aim of this section is to present the possible increase in data interpretation and
related methods.
Organizer: Dr. Jan Urban, Laboratory of Signal and Image Processing, Institute of
Complex Systems, Faculty of Fisheries and Protection of Waters, University of
South Bohemia.
– SS3: Half-Day GATB Tutorial. The Genome Analysis Toolbox with de Bruijn
Graph
The GATB programming day is an educational event organized by the GATB team.
This free event is open to everyone who is familiar in C++ programming and wants
to learn how to create NGS data analysis software.
The tutorial has a focus on the high-performance GATB-Core library and is taught
by the developers of this library.
During this half-day tutorial, some of the following topics are explored:
• A theoretical introduction to GATB: the basic concepts.
• GATB-Core practical coding session 1: I/O operations on reads files.
• The GATB de Bruijn graph API.
• GATB-Core practical coding session 2: k-mer and graph APIs in action.
• GATB-Core practical coding session 3: writing a short read corrector tool.
• Q and A session: obtain answers from GATB experts.
VIII Preface

Organizers: Dr. Dominique Lavenier, GenScale Team Leader, Inria/IRISA, Cam-

pus de Beaulieu, Rennes, France.
Dr. Patrick Durand, Inria, Genscale Team, Campus de Beaulieu, Rennes, France.
– SS4: Medical Planning: Operating Theater Design and Its Impact on Cost,
Area, and Workflow
The design of operating rooms is one of the most complicated tasks of hospital
design because of its characteristics and requirements. Patients, staff, and tools
should have determined passes through the operating suite. Many hospitals assume
that the operating suite is the most important unit in the hospital because of its
high–revenue. Arch design of these suites is a very critical point in solving an
optimized problem in spaces, workflow of clean, dirty, and patient in/out in addition
to staff together with their relations with adjacent departments. In this session, we
illustrate the most common designs of operating suites and select the most suitable
one to satisfy the effectiveness of the operating suite, maximizing throughput,
minimizing the costs, and decreasing the required spaces related to available
resources/possibilities. The design should comply with country guidelines, infection
control rules, occupational safety and health, and satisfy the maximum benefits for
patients and staff. A comparative study was performed on 15 hospitals and it
recorded that the single input–output technique is the best design.
Motivation and objectives for the session: Operating suite design is a very critical
task owing to its impact. Biomedical engineers should participate in the design and
review the workflow and available functions.
Organizer: Dr. Khaled El-Sayed, Department of Electrical and Medical Engi-
neering at Benha University, Egypt
– SS5: Challenges Representing Large-Scale Biological Data
Visualization models have been shown to be remarkably important in the inter-
pretation of datasets across many fields of study. In the context of bioinformatics
and computational biology various tools have been proposed to visualize molecular
data and help understand how biological systems work. Despite that, several
challenges still persist when faced with complex and dynamic data and major
advances are required to correctly manage the multiple dimensions of the data.
The aim of this special session is to bring together researchers to present recent and
ongoing research activities related to advances in visualization techniques and tools,
focused on any major molecular biology problem, with the aim of allowing for the
exchange and sharing of proposed ideas and experiences with novel visualization
metaphors.
Topics of interest include:
• Genome and sequence data
• Omics data (transcriptomics, proteomics, metabolomics)
• Biological networks and pathways
• Time-series data
• Biomedical ontologies
• Macromolecular complexes
• Phylogenetic data
 Preface IX

• Biomarker discovery
• Integration of image and omics data for systems biology
• Modeling and simulation of dynamical processes
Organizer: Prof. Joel P. Arrais FCTUC – University of Coimbra, Portugal.
– SS6: Omics of Space Travelled Microbes – Bioinformatics and Biomedical
Aspects
The National Research Council (NRC) Committee for the Decadal Survey on
Biological and Physical Sciences in Space reported that “microbial species that are
uncommon, or that have significantly increased or decreased in number, can be
studied in a “microbial observatory” on the International Space Station (ISS).” As
part of the microbial observatory effort the NRC decadal survey committee sug-
gested that NASA should: “(a) capitalize on the technological maturity, low cost,
and speed of genomic analyses and the rapid generation time of microbes to monitor
the evolution of microbial genomic changes in response to the selective pressures
present in the spaceflight environment; (b) study changes in microbial populations
from the skin and feces of the astronauts, plant and plant growth media, and
environmental samples taken from surfaces and the atmosphere of the ISS; and
(c) establish an experimental program targeted at understanding the influence of the
spaceflight environment on defined microbial populations.”
The proposed session discusses state-of-the-art molecular techniques, bioinfor-
matics tools, and their benefit in answering the astronauts and others who live in
closed systems.
Organizer: Dr. Kasthuri Venkateswaran, Senior Research Scientist, California
Institute of Technology, Jet Propulsion Laboratory, Biotechnology and Planetary
Protection Group, Pasadena, CA
– SS7: Data-Driven Biology – New Tools, Techniques, and Resources
Advances in sequencing techniques have accelerated data generation at diverse
regulatory levels in an unprecedented way. The challenge now is to integrate these
data to understand regulation at a systems level. As the sequencing technologies
evolve, new tools and resources follow, revealing new aspects of complex bio-
logical systems.
This special session brings together experts from computational biology and
machine learning to present recent advances in the development of new tools and
resources using next-generation sequencing data including novel emerging fields
such as single-cell transcriptomics. The session features an invited speaker and
three/four short talks. To promote emerging leaders of the field, we select invited
speakers who have gained their independence in recent years.
Organizer: Dr. Joshi Anagha, Division of Developmental Biology at the Roslin
Institute, University of Edinburgh
– SS8: Smart Sensor and Sensor-Network Architectures
There is a significant demand for tools and services supporting rehabilitation,
well-being and healthy life styles while reducing the level of intrusiveness as well as
increasing real-time available and reliable results. For example, self-monitoring
X Preface

applications need to be improved to move beyond tracking exercise habits and

capture a more comprehensive digital footprint of human behavior. This session
focuses on primary parameter capturing devices and networks demonstrating
advances in sensor development including a customized algorithmic shell research
to support diagnostic decisions. Target domains are, for example, continuous dif-
ferentiating between mental and physical stress, blood pressure monitoring, sleep
quality monitoring, HRV etc.
Organizers: Prof. Dr. Natividad Martinez, Internet of Things Laboratory, Reut-
lingen University, Germany.
Prof. Dr. Juan Antonio Ortega, University of Seville, Spain.
Prof. Dr. Ralf Seepold, Ubiquitous Computing Lab, HTWG Konstanz, Germany.
– SS9: High-Throughput Bioinformatic Tools for Genomics
Genomics is concerned with the sequencing and analysis of an organism’s genome.
It is involved in the understanding of how every single gene can affect the entire
genome. This goal is mainly afforded using the current, cost-effective,
high-throughput sequencing technologies. These technologies produce a huge
amount of data that usually require high-performance computing solutions and
opens new ways for the study of genomics, but also transcriptomics, gene
expression, and systems biology, among others. The continuous improvements and
broader applications on sequencing technologies is producing an ongoing demand
for improved high-throughput bioinformatics tools.
Therefore, we invite authors to submit original research, new tools or pipelines, or
their update, and review articles on topics helping in the study of genomics in the
wider sense, such as (but not limited to):
• Tools for data pre-processing (quality control and filtering)
• Tools for sequence mapping
• Tools for de novo assembly
• Tools for quality check of sequence assembling
• Tools for the comparison of two read libraries without an external reference
• Tools for genomic variants (such as variant calling or variant annotation)
• Tools for functional annotation: identification of domains, orthologues, genetic
markers, controlled vocabulary (GO, KEGG, InterPro)
• Tools for biological enrichment in non-model organisms
• Tools for gene expression studies
• Tools for Chip-Seq data
• Tools for “big-data” analyses
• Tools for handling and editing complex workflows and pipelines
• Databases for bioinformatics
Organizers: Prof. M. Gonzalo Claros, Department of Molecular Biology and
Biochemistry, University of Málaga, Spain.
Dr. Javier Pérez Florido, Bioinformatics Research Area, Fundación Progreso y
Salud, Seville, Spain.
 Preface XI

– SS10: Systems Biology Approaches to Decipher Long Noncoding RNA–Protein

Associations
Long noncoding RNAs (lncRNAs) make up large amounts of the RNA and total
genomic repertoire. Studies on the functional characterization of lncRNAs have
resulted in data on interactions with their RNA peers, DNA, or proteins. Although
there has been an increase in evidence on the link between lncRNAs and diverse
human diseases, there is a dearth of lncRNA–protein association studies. Addi-
tionally, existing methods do not provide theories about the possible molecular
causes of such associations linking to diseases. How such regulatory interactions
between classes of lncRNAs and proteins would have a significant influence on the
organism and disease remains a challenge. A good number of bioinformatics
approaches have arisen in the recent past exploring these challenges. The idea
behind this session is to bring together the wide gamut of researchers who have
worked on these methods across different organisms.
The following are the sub-topics of the proposed session, which we would like to
call for papers.
• LncRNAs in genomes: annotation and curation
• LncRNA–protein interactions leading to important diseases: systems Biology
approaches
• Identifying lncRNAs with respect to their mechanism and dysregulation in
diseases
• LncRNA databases and webservers
• Machine learning approaches and prediction servers
Organizer: Prashanth Suravajhala, PhD, Department of Biotechnology and
Bioinformatics, Birla Institute of Scientific Research, India
– SS11: Gamified Rehabilitation for Disabled People
Gamification is a hot topic in many areas as it aims at motivating people to do
things driven by different innate needs like the wish to accomplish tasks, to compete
against others, or to gain something. These and other motivators are efficiently
applied in computer games and could be extraordinarily useful in ensuring that
patients perform their daily exercises regularly and have fun.
The idea of exergames (exercise games) is not new; the literature reveals that much
effort has already been made and with great success. Nevertheless, most applica-
tions have been developed for special problems or diseases (i.e., stroke, parkinson,
cerebral palsy etc.) and are not generally applicable. In general, people suffering
from severe disabilities and chronic diseases are rarely addressed as a target
group. Also, the focus is generally set on the medical achievements, which is
correct, but the next step would be to enhance the fun factor because no tool is of
much use if the patient is not using it because of boredom or demotivation.
The objective of this special session is to gather new ideas about the combination of
need and fun, i.e., find ways to create exercise platforms that fit everybody’s needs,
provide access to the therapist for monitoring and configuration, while the patients
benefit physically and mentally when having a good time.
Target groups would be people of:
XII Preface

• All ages, while focusing on younger people, who can be involved more easily
but are less addressed in the literature
• All diseases, while focusing on chronic illness and severe disabilities (e.g.,
muscle dystrophies and atrophies)
The contributions should show advances in at least one of the following areas:
• Adaptability to users with all kinds of problems (e.g., possibility to configure the
limbs used to play or playing with facial movements, wheelchair and standing
modes, coping with muscle weaknesses etc.)
• Implementation of gaming techniques and special motivators
• Physical or mental exercises, aimed at rehabilitation or daily practice
• Understanding the users, awareness of their level of motivation, fatigue or
progress and react accordingly
Organizer: Dr. Martina Eckert, Associate Professor at University of Madrid,
Spain.
– SS12: Modelling of Glucose Dynamics for Diabetes
Diabetes is the eighth most common cause of death, while its treatment relies on
technology to process continuously measured glucose levels.
Organizer: Dr. Tomas Koutny, Faculty of Applied Sciences, University of West
Bohemia
– SS13: Biological Network Analysis in Multi-omics Data Integration
In many biological applications, multiple data types may be produced to determine
the genetics, epigenetics, and microbiome affecting gene regulation and metabo-
lism. Although producing multiple data types should provide a more complete
description of the processes under study due to multiple factors such as study design
(synchronization of data production, number of samples, varying conditions), the
analysis may leave more unfulfilled promises than synergy expected from the
wealth of data.
In this session, some of the following challenges are addressed:
• How to conduct meaningful meta-analysis on historical data.
• How to use biological knowledge (represented in reproducible and interoperable
manner) in the analysis of large and sparse data sets more effectively.
• How to fill the gap between hypothesis-driven mechanistic studies, e.g.,
applying modelling to very well studied biochemical processes and data-driven
hypothesis-free approaches. How omics data can help.
• Beyond meta-transcriptomics and metagenomics: integration and interpretation
of microbiome and host data.

We would like to bring together communities concerned with these topics to pre-
sent state-of-the-art and current cutting-edge developments, preferably work under
construction or published within the past year.
 Preface XIII

• Objective 1: presentation and discussion of newest methods

• Objective 2: round-table discussions on the topics highlighted above and other
related topics suggested by session participants

An additional topic that does not fit the proposed session but that I would love to see
addressed is: How to improve open access to the data that is not next-generation
sequencing (e.g., metabolomics, proteomics, plant phenotyping). For this an active
participation of journal editors would be necessary to discuss opportunities to change
journal publication policies.
Organizer: Dr. Wiktor Jurkowski, Jurkowski Group, Earlham Institute, Norwich
Research Park, UK
– SS14: Oncological Big Data and New Mathematical Tools
Current scientific methods produce various omics data sets covering many cellular
functions. However, these data sets are commonly processed separately owing to
limited ways in how to connect different omics data together for a meaningful
analysis. Moreover, it is currently a problem to integrate such data into mathe-
matical models. We are entering the new era of biological research where the main
problem is not to obtain the data but to process and analyze them. In this regard, a
strong mathematical approach can be very effective (see J. Gunawardena’s essay
“Models in biology: accurate descriptions of our pathetic thinking,” BMC Biology
2014, 12:29).
In this Special Session we focus on big data (omics and biological pathways) related
to oncological research. General biological processes that are relevant to cancer can
also be studied. Mathematical tools basically mean statistical learning (data mining,
inference, prediction), modeling, and simulation. We want to place a special
emphasis on causality. Closely tied to mathematical tools, efficient computational
tools can be considered.
Organizers: Dr. Gregorio Rubio, Instituto de Matematica Multidisciplinar,
Universitat Politecnica de Valencia, Valencia, Spain. Dr. Rafael Villanueva,
Instituto de Matematica Multidisciplinar, Universitat Politecnica de Valencia,
Valencia, Spain.
In this edition of IWBBIO, we were honored to have the following invited speakers:
1. Prof. Roderic Guigo, Coordinator of Bioinformatics and Genomics at Centre de
Regulacio Genomica (CRG). Head of the Computational Biology of RNA Pro-
cessing Group. Universitat Pompeu Fabra, Barcelona, Spain
2. Prof. Joaquin Dopazo, Director of the Computational Genomics Department, Centro
de Investigación Príncipe Felipe- CIPF, Valencia, Spain
3. Prof. Jose Antonio Lorente, Director of Centre for Genomics and Oncological
Research (GENYO). Professor of Legal and Forensic Medicine, University of
Granada, Spain
It is important to note, that for the sake of consistency and readability of the book, the
presented papers are classified under 16 chapters. The organization of the papers is in
two volumes arranged following the topics list included in the call for papers. The first
XIV Preface

volume (LNBI 10208), entitled “Advances in Computational Intelligence: Part I” is

divided into seven main parts and includes the contributions on:
1. Advances in computational intelligence for critical care
2. Bioinformatics for health care and diseases
3. Biomedical engineering
4. Biomedical image analysis
5. Biomedical signal analysis
6. Biomedicine
7. Challenges representing large-scale biological data
The second volume (LNBI 10209), entitled “Advances in Computational Intelligence:
Part II” is divided into nine main parts and includes the contributions on:
1. Computational genomics
2. Computational proteomics
3. Computational systems for modelling biological processes
4. Data-driven biology: new tools, techniques, and resources
5. E-health
6. High-throughput bioinformatic tools for genomics
7. Oncological big data and new mathematical tools
8. Smart Sensor and sensor-network architectures
9. Time-lapse experiments and multivariate biostatistics
This fifth edition of IWBBIO was organized by the Universidad de Granada together
with the Spanish Chapter of the IEEE Computational Intelligence Society. We wish to
thank to our main sponsor and the Faculty of Science, Department of Computer
Architecture and Computer Technology and CITIC-UGR, from the University of
Granada, for their support and grants. We also wish to thank the Editors-in-Chief of
different international journals for their interest in having special issues with the best
papers of IWBBIO.
We would also like to express our gratitude to the members of the different
committees for their support, collaboration, and good work. We especially thank the
local Organizing Committee, Program Committee, the reviewers, and special session
organizers. Finally, we want to thank Springer, and especially Alfred Hofmann and
Anna Kramer, for their continuous support and cooperation.

April 2017 Ignacio Rojas

Francisco Ortuño
 Organization

Steering Committee
Miguel A. Andrade University of Mainz, Germany
Hesham H. Ali University of Nebraska, USA
Oresti Baños University of Twente, the Netherlands
Alfredo Benso Politecnico di Torino, Italy
Giorgio Buttazzo Superior School Sant’Anna, Italy
Mario Cannataro University Magna Graecia of Catanzaro, Italy
Jose María Carazo Spanish National Center for Biotechnology (CNB), Spain
Jose M. Cecilia Universidad Católica San Antonio de Murcia, Spain
M. Gonzalo Claros University of Malaga, Spain
Joaquin Dopazo Research Center Principe Felipe, Spain
Werner Dubitzky University of Ulster, UK
Afshin Fassihi Universidad Católica San Antonio de Murcia, Spain
Jean-Fred Fontaine University of Mainz, Germany
Humberto Gonzalez University of the Basque Country, Spain
Concettina Guerra College of Computing, Georgia Tech, USA
Andy Jenkinson Karolinska Institute, Sweden
Craig E. Kapfer Reutlingen University, Germany
Narsis Aftab Kiani European Bioinformatics Institute, UK
Natividad Martinez Reutlingen University, Germany
Marco Masseroli Politechnical University of Milan, Italy
Federico Moran Complutense University of Madrid, Spain
Cristian R. Munteanu University of A Coruña, Spain
Jorge A. Naranjo New York University, Abu Dhabi
Michael Ng Hong Kong Baptist University, SAR China
Jose L. Oliver University of Granada, Spain
Juan Antonio Ortega University of Seville, Spain
Julio Ortega University of Granada, Spain
Alejandro Pazos University of A Coruña, Spain
Javier Perez Florido Genomics and Bioinformatics Platform of Andalusia,
Spain
Violeta I. Pérez Nueno Inria Nancy Grand Est, LORIA, France
Horacio Pérez-Sánchez Universidad Católica San Antonio de Murcia, Spain
Alberto Policriti University of Udine, Italy
Omer F. Rana Cardiff University, UK
M. Francesca Romano Superior School Sant’Anna, Italy
Yvan Saeys VIB - Ghent University, Belgium
Vicky Schneider The Genome Analysis Centre, UK
Ralf Seepold HTWG Konstanz, Germany
XVI Organization

Mohammad Soruri University of Birjand, Iran

Yoshiyuki Suzuki Tokyo Metropolitan Institute of Medical Science, Japan
Oswaldo Trelles University of Malaga, Spain
Renato Umeton CytoSolve Inc., USA
Jan Urban University of South Bohemia, Czech Republic
Alfredo Vellido Polytechnical University of Catalonia, Spain

Program Committee

Jesus S. Aguilar Carlos Cano

Carlos Alberola Rita Casadio
Hisham Al-Mubaid Daniel Castillo
Rui Carlos Alves Ting-Fung Chan
Yuan An Nagasuma Chandra
Georgios Anagnostopoulos Kun-Mao Chao
Eduardo Andrés León Bernard Chen
Antonia Aránega Bolin Chen
Saúl Ares Brian Chen
Masanori Arita Chuming Chen
Ruben Armañanzas Jie Chen
Joel P. Arrais Yuehui Chen
Patrizio Arrigo Jianlin Cheng
O. Bamidele Awojoyogbe Shuai Cheng
Jaume Bacardit I-Jen Chiang
Hazem Bahig Jung-Hsien Chiang
Pedro Ballester Young-Rae Cho
Graham Balls Justin Choi
Ugo Bastolla Darrell Conklin
Sidahmed Benabderrahmane Clare Coveney
Steffanny A. Bennett Aedin Culhane
Daniel Berrar Miguel Damas
Mahua Bhattcharya Antoine Danchin
Concha Bielza Bhaskar DasGupta
Armando Blanco Ricardo De Matos
Ignacio Blanquer Guillermo de la Calle
Paola Bonizzoni Javier De Las Rivas
Christina Boucher Fei Deng
Hacene Boukari Marie-Dominique Devignes
David Breen Ramón Diaz-Uriarte
Fiona Browne Julie Dickerson
Dongbo Bu Ye Duan
Jeremy Buhler Beatrice Duval
Keith C.C. Saso Dzeroski
Gabriel Caffarena Khaled El-Sayed
Anna Cai Mamdoh Elsheshengy
 Organization XVII

Christian Exposito Sun Kim

Weixing Feng Kengo Kinoshita
Jose Jesús Fernandez Ekaterina Kldiashvili
Gionata Fragomeni Jun Kong
Xiaoyong Fu Tomas Koutny
Juan Manuel Galvez Natalio Krasnogor
Alexandre G.de Brevern Abhay Krishan
Pugalenthi Ganesan Marija Krstic-Demonacos
Jean Gao Sajeesh Kumar
Rodolfo Garcia Lukasz Kurgan
Mark Gerstein Stephen Kwok-Wing
Razvan Ghinea Istvan Ladunga
Daniel Gonzalez Peña T.W. Lam
Dianjing Guo Pedro Larrañaga
Jun-tao Guo Dominique Lavenier
Maozu Guo Jose Luis Lavin
Christophe Guyeux Doheon Lee
Michael Hackenberg Xiujuan Lei
Michiaki Hamada André Leier
Xiyi Hang Kwong-Sak Leung
Jin-Kao Hao Chen Li
Nurit Haspel Dingcheng Li
Morihiro Hayashida Jing Li
Jieyue He Jinyan Li
Pheng-Ann Heng Min Li
Luis Javier Herrera Xiaoli Li
Pietro Hiram Yanpeng Li
Lynette Hirschman Li Liao
Ralf Hofestadt Hongfei Lin
Vasant Honavar Hongfang Liu
Wen-Lian Hsu Jinze Liu
Jun Hu Xiaowen Liu
Xiaohua Hu Xiong Liu
Jun Huan Zhenqiu Liu
Chun-Hsi Huang Zhi-Ping Liu
Heng Huang Rémi Longuespée
Jimmy Huang Miguel Angel Lopez Gordo
Jingshan Huang Ernesto Lowy
Seiya Imoto Jose Luis
Anthony J. Kusalik Suryani Lukman
Yanqing Ji Feng luo
Xingpeng Jiang Bernard M.E.
Mingon Kang Bin Ma
Dong-Chul Kim Qin Ma
Dongsup Kim Malika Mahoui
Hyunsoo Kim Alberto Maria
XVIII Organization

Tatiana Marquez-Lago Kunihiko Sadakane

Keith Marsolo Michael Sadovsky
Francisco Martinez Alvarez Belen San Roman
Osamu Maruyama Maria Jose Saez
Tatiana Maximova Hiroto Saigo
Roderik Melnik José Salavert
Jordi Mestres Carla Sancho Mestre
Hussain Michelle Emmanuel Sapin
Ananda Mondal Kengo Sato
Antonio Morreale Jean-Marc Schwartz
Walter N. Moss Russell Schwartz
Maurice Mulvenna Jose Antonio Seoane
Enrique Muro Xuequn Shang
Radhakrishnan Nagarajan Piramanayagam Shanmughavel
Vijayaraj Nagarajan Xiaoman Shawn
Kenta Nakai Xinghua Shi
Isabel A. Nepomuceno Tetsuo Shibuya
Mohammad Nezami Dong-Guk Shin
Anja Nohe Amandeep Sidhu
Michael Ochs Istvan Simon
Baldomero Oliva Richard Sinnott
Jose Luis Oliveira Jiangning Song
Motonori Ota Joe Song
David P. Zhengchang Su
Tun-Wen Pai Joakim Sundnes
Paolo Paradisi Wing-Kin Sung
Hyun-Seok Park Prashanth Suravajhala
Kunsoo Park Martin Swain
Taesung Park Sing-Hoi Sze
David Pelta Mehmet Tan
Alexandre Perera Xing Tan
María Del Mar Pérez Gómez Li Teng
Vinhthuy Phan Tianhai Tian
Antonio Pinti Pedro Tomas
Héctor Pomares Carlos Toro
Mihail Popescu Carolina Torres
Benjarath Pupacdi Paolo Trunfio
Sanguthevar Rajasekaran Esko Ukkonen
Shoba Ranganathan Lucia Vaira
Jairo Rocha Paola Velardi
Fernando Rojas Julio Vera
Jianhua Ruan Konstantinos Votis
Gregorio Rubio Slobodan Vucetic
Antonio Rueda Ying-Wooi Wan
Irena Rusu Chong Wang
Vincent Shin-Mu Tseng Haiying Wang
 Organization XIX

Jason Wang Jingkai Yu

Jian Wang Hong Yue
Jianxin Wang Erliang Zeng
Jiayin Wang Xue-Qiang Zeng
Junbai Wang Aidong Zhang
Junwen Wang Chi Zhang
Lipo Wang Jin Zhang
Li-San Wang Jingfen Zhang
Lusheng Wang Kaizhong Zhang
Yadong Wang Shao-Wu Zhang
Yong Wang Xingan Zhang
Ka-Chun Wong Zhongming Zhao
Ling-Yun Wu Huiru Zheng
Xintao Wu Bin Zhou
Zhonghang Xia Shuigeng Zhou
Fang Xiang Xuezhong Zhou
Lei Xu Daming Zhu
Zhong Xue Dongxiao Zhu
Patrick Xuechun Shanfeng Zhu
Hui Yang Xiaoqin Zou
Zhihao Yang Xiufen Zou

Additional Reviewers

Alquezar, Rene Moulin, Serge

Amaya Vazquez, Ivan Olier, Iván
Belanche, Lluís Ortega-Martorell, Sandra
Browne, Fiona Ribas, Vicent
Calabrese, Barbara Scherz, Wilhelm Daniel
Cho, Ken Sebastiani, Laura
Coelho, Edgar Seong, Giun
Datko, Patrick Smith, Peter
Gaiduk, Maksym Treepong, Panisa
Gonzalez-Abril, Luis Tu, Shikui
Ji, Guomin Valenzuela, Olga
Kang, Hong Vizza, Patrizia
Langa, Jorge Wu, Yonghui
Liang, Zhewei Yan, Shankai
Martin-Guerrero, Jose D. Zucco, Chiara
 Contents – Part II

Computational Genomics

Investigation of DNA Sequences Utilizing Frequency-Selective

Nanopore Structures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Ali Hilal-Alnaqbi, Mahmoud Al Ahmad, Tahir A. Rizvi,
and Farah Mustafa

Uropathogenic Escherichia coli: An Ideal Resource for DNA Microarray

Probe Designing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Payam Behzadi and Elham Behzadi

Lost Strings in Genomes: What Sense Do They Make? . . . . . . . . . . . . . . . . 20

Michael Sadovsky, Jean-Fred Fontaine, Miguel A. Andrade-Navarro,
Yury Yakubailik, and Natalia Rudenko

Comprehensive Study of Instable Regions in Pseudomonas Aeruginosa . . . . . 30

Dan Wang, Jingyu Li, and Lusheng Wang

Breathogenomics: A Computational Architecture for Screening,

Early Diagnosis and Genotyping of Lung Cancer . . . . . . . . . . . . . . . . . . . . 41
Emmanuel Adetiba, Marion O. Adebiyi, and Surendra Thakur

Mixed-Integer Programming Model for Profiling Disease Biomarkers

from Gene Expression Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
André M. Santiago, Miguel Rocha, António Dourado, and Joel P. Arrais

Assembly of Gene Expression Networks Based on a Breast

Cancer Signature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Dimitrios Apostolos Chalepakis Ntellis, Ekaterini S. Bei,
Dimitrios Kafetzopoulos, and Michalis Zervakis

Pairwise and Incremental Multi-stage Alignment of Metagenomes:

A New Proposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Esteban Pérez-Wohlfeil, Oscar Torreno, and Oswaldo Trelles

An Expanded Association Approach for Rare Germline Variants

with Copy-Number Alternation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Yu Geng, Zhongmeng Zhao, Daibin Cui, Tian Zheng, Xuanping Zhang,
Xiao Xiao, and Jiayin Wang

Parallelizing Partial Digest Problem on Multicore System. . . . . . . . . . . . . . . 95

Hazem M. Bahig, Mostafa M. Abbas, and M.M. Mohie-Eldin
XXII Contents – Part II

Computational Proteomics

Prediction of Calmodulin-Binding Proteins Using Short-Linear Motifs . . . . . . 107

Yixun Li, Mina Maleki, Nicholas J. Carruthers, Luis Rueda,
Paul M. Stemmer, and Alioune Ngom

Data Mining the Protein Data Bank to Identify and Characterise Chameleon
Coil Sequences that Form Symmetric Homodimer b-Sheet Interfaces. . . . . . . 118
Johanna Laibe, Melanie Broutin, Aaron Caffrey, Barbara Pierscionek,
and Jean-Christophe Nebel

Associating Gene Ontology Terms with Pfam Protein Domains . . . . . . . . . . 127

Seyed Ziaeddin Alborzi, Marie-Dominique Devignes,
and David W. Ritchie

3D Protein-Structure-Oriented Discovery of Clinical Relation Across

Chronic Lymphocytic Leukemia Patients . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Konstantinos Mochament, Andreas Agathangelidis,
Eleftheria Polychronidou, Christos Palaskas, Elias Kalamaras,
Panagiotis Moschonas, Kostas Stamatopoulos, Anna Chailyan,
Nanna Overby, Paolo Marcatili, Anastasia Hadzidimitriou,
and Dimitrios Tzovaras

Application of a Membrane Protein Structure Prediction Web Service

GPCRM to a Gastric Inhibitory Polypeptide Receptor Model . . . . . . . . . . . . 151
Ewelina Rutkowska, Przemyslaw Miszta, Krzysztof Mlynarczyk,
Jakub Jakowiecki, Pawel Pasznik, Slawomir Filipek, and Dorota Latek

In Silico Prediction of 3D Structure of Anopheles Gambiae

ABCC12 Protein. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Marion O. Adebiyi, Efejiro Ashano, and Emmanuel Adetiba

Exploring Symmetric Substructures in Protein Interaction Networks

for Pairwise Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
Ahed Elmsallati, Swarup Roy, and Jugal K. Kalita

Identification and in silico Analysis of Glutathione Reductase Transcripts

Expressed in Olive (Olea europaea L.) Pollen and Pistil . . . . . . . . . . . . . . . 185
Estefanía García-Quirós, Rosario Carmona, Adoración Zafra,
M. Gonzalo Claros, and Juan de Dios Alché

Computational Systems for Modelling Biological Processes

Cyber Immunity: A Bio-Inspired Cyber Defense System . . . . . . . . . . . . . . . 199

Peter Wlodarczak
 Contents – Part II XXIII

An Accurate Database of the Fixation Probabilities for All Undirected

Graphs of Order 10 or Less . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209
Fernando Alcalde Cuesta, Pablo González Sequeiros,
Álvaro Lozano Rojo, and Rubén Vigara Benito

Quantum Computing Based Inference of GRNs . . . . . . . . . . . . . . . . . . . . . 221

Abhinandan Khan, Goutam Saha, and Rajat Kumar Pal

Analysis of Soil Data from Eastern of Morocco Based

on Data Mining Process. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 234
Imane Belabed, Mohammed Talibi Alaoui, Abdelmajid Belabed,
and Youssef Talibi Alaoui

A Multi-objective Optimization Framework for Multiple Sequence

Alignment with Metaheuristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
Cristian Zambrano-Vega, Antonio J. Nebro, José García-Nieto,
and José F. Aldana-Montes

Analysis of Gene Expression Discretization Techniques

in Microarray Biclustering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
J.S. Dussaut, C.A. Gallo, J.A. Carballido, and I. Ponzoni

Analysis of Informative Features for Negative Selection in Protein

Function Prediction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
Marco Frasca, Fabio Lipreri, and Dario Malchiodi

Differential Network Analysis of Anti-sense Regulation . . . . . . . . . . . . . . . . 277

Marc Legeay, Béatrice Duval, and Jean-Pierre Renou

Nonlinear Control and Simulation of a Dielectric Elastomer Actuator-Based

Compression Bandage on Flexible Human Calf . . . . . . . . . . . . . . . . . . . . . 289
Shahram Pourazadi, Mehrdad Moallem, and Carlo Menon

Dengue Agent-Based Model in South American Temperate Zone . . . . . . . . . 301

Carlos M. Pais, Maximiliano G. Colazo, Maximiliano Fernandez,
Silvana Bulatovich, and Hugo Fernandez

Data Driven Biology - New Tools, Techniques and Resources

Transcription Control in Human Cell Types by Systematic Analysis

of ChIP Sequencing Data from the ENCODE . . . . . . . . . . . . . . . . . . . . . . . 315
Guillaume Devailly and Anagha Joshi

Management of Data Structures Generated During Simulations

of the Evolution of Multicellular Systems. . . . . . . . . . . . . . . . . . . . . . . . . . 325
Andreea Robu, Mihaela Crisan-Vida, Nicolae Robu, and Adrian Neagu
XXIV Contents – Part II

Finding Transcripts Associated with Prostate Cancer Gleason Stages Using

Next Generation Sequencing and Machine Learning Techniques . . . . . . . . . . 337
Osama Hamzeh, Abedalrhman Alkhateeb, Iman Rezaeian, Aram Karkar,
and Luis Rueda

Bioinformatics from a Big Data Perspective: Meeting the Challenge . . . . . . . 349

Francisco Gomez-Vela, Aurelio López, José A. Lagares,
Domingo S. Baena, Carlos D. Barranco, Miguel García-Torres,
and Federico Divina

Computational Prediction of Host-Pathogen Interactions Through Omics

Data Analysis and Machine Learning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360
Diogo Manuel Carvalho Leite, Xavier Brochet, Grégory Resch,
Yok-Ai Que, Aitana Neves, and Carlos Peña-Reyes

Simultaneous Gene Selection and Weighting in Nearest Neighbor Classifier

for Gene Expression Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 372
Antonio Alarcón-Paredes, Gustavo Adolfo Alonso, Eduardo Cabrera,
and René Cuevas-Valencia

Machine Learning Approaches for Predicting High Utilizers

in Health Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 382
Chengliang Yang, Chris Delcher, Elizabeth Shenkman,
and Sanjay Ranka

eHealth

A Device Supporting the Self Management of Tinnitus . . . . . . . . . . . . . . . . 399

Pablo Chamoso, Fernando De La Prieta, Alberto Eibenstein,
Daniel Santos-Santos, Angelo Tizio, and Pierpaolo Vittorini

A Case Study on the Integration of Heterogeneous Data Sources

in Public Health . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 411
Pierpaolo Vittorini, Anna Maria Angelone, Vincenza Cofini,
Leila Fabiani, Antonella Mattei, and Stefano Necozione

QSAR Classification Models for Predicting Affinity to Blood

or Liver of Volatile Organic Compounds in e-Health . . . . . . . . . . . . . . . . . . 424
Fiorella Cravero, María Jimena Martínez, Mónica F. Díaz,
and Ignacio Ponzoni

Usage of VR Headsets for Rehabilitation Exergames . . . . . . . . . . . . . . . . . . 434

Martina Eckert, José Zarco, Juan Meneses, and José-Fernán Martínez
 Contents – Part II XXV

High-Throughput Bioinformatic Tools for Genomics

Search of Regions with Periodicity Using Random Position Weight

Matrices in the Genome of C. elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . 445
E.V. Korotkov and M.A. Korotkova

RNA Sequencing Analysis of Neural Cell Lines: Impact of Normalization

and Technical Replication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 457
V. Bleu Knight and Elba E. Serrano

Towards a Universal Genomic Positioning System: Phylogenetics

and Species IDentification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 469
Max H. Garzon and Sambriddhi Mainali

A High Performance Storage Appliance for Genomic Data. . . . . . . . . . . . . . 480

Gaurav Kaul, Zeeshan Ali Shah, and Mohamed Abouelhoda

Obtaining the Most Accurate de novo Transcriptomes for Non-model

Organisms: The Case of Castanea sativa . . . . . . . . . . . . . . . . . . . . . . . . . . 489
Marina Espigares, Pedro Seoane, Rocío Bautista, Julia Quintana,
Luis Gómez, and M. Gonzalo Claros

Accelerating Smith-Waterman Alignment of Long DNA Sequences

with OpenCL on FPGA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500
Enzo Rucci, Carlos Garcia, Guillermo Botella, Armando De Giusti,
Marcelo Naiouf, and Manuel Prieto-Matias

Smith-Waterman Acceleration in Multi-GPUs:

A Performance per Watt Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 512
Jesús Pérez Serrano, Edans Flavius De Oliveira Sandes,
Alba Cristina Magalhaes Alves de Melo, and Manuel Ujaldón

A Deep Learning Network for Exploiting Positional Information

in Nucleosome Related Sequences. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 524
Mattia Antonino Di Gangi, Salvatore Gaglio, Claudio La Bua,
Giosué Lo Bosco, and Riccardo Rizzo

Oncological Big Data and New Mathematical Tools

A Clinical Tool for Automated Flow Cytometry Based on Machine

Learning Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 537
Claude Takenga, Michael Dworzak, Markus Diem, Rolf-Dietrich Berndt,
Erling Si, Michael Brandstoetter, Leonid Karawajew, Melanie Gau,
and Martin Kampel
XXVI Contents – Part II

Prognostic Modeling and Analysis of Tumor Response to Fractionated

Radiotherapy for Patients with Squamous Cell Lung Cancer. . . . . . . . . . . . . 549
Hualiang Zhong, Hoda Sharifi, Haisen Li, Weihua Mao,
and Indrin J. Chetty

Uncertainty Quantification for Meningococcus B Carriers Prediction . . . . . . . 560

Luis Acedo, Clara Burgos, Juan-Carlos Cortés, and Rafael J. Villanueva

Smart Sensor and Sensor-Network Architectures

A Novel Wearable for Rehabilitation Using Infrared Sensors:

A Preliminary Investigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 573
Jordan Lui, Andrea Ferrone, Zhi Yih Lim, Lorenzo Colace,
and Carlo Menon

Real Time Localization Using Bluetooth Low Energy . . . . . . . . . . . . . . . . . 584

Massimo Conti

A Sensor Grid for Pressure and Movement Detection Supporting Sleep

Phase Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 596
Maksym Gaiduk, Ina Kuhn, Ralf Seepold, Juan Antonio Ortega,
and Natividad Martínez Madrid

A Portable Wireless sEMG and Inertial Acquisition System for Human

Activity Monitoring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 608
Giorgio Biagetti, Paolo Crippa, Laura Falaschetti, Simone Orcioni,
and Claudio Turchetti

An Automatic and Intelligent System for Integrated Healthcare

Processes Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 621
Virginia Cid-de-la-Paz, Andrés Jiménez-Ramírez, and M.J. Escalona

A Microcontroller Based System for Controlling Patient

Respiratory Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 631
Leticia Morales, Manuel Domínguez Morales, Andrés Jiménez-Ramírez,
and M.J. Escalona

Requirements Analysis for User Interfaces in Mobile eHealth Applications. . . 642

Armando Statti and Natividad Martinez Madrid

Mobile Health System for Evaluation of Breast Cancer Patients During

Treatment and Recovery Phases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 653
Joaquin Ollero, Jose Antonio Moral-Munoz, Ignacio Rojas,
and Oresti Banos
 Contents – Part II XXVII

Key Factors for Innovative Developments on Health

Sensor-Based System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 665
Maria Dolores Peláez, Miguel López-Medina, Macarena Espinilla,
and Javier Medina-Quero

Time Lapse Experiments and Multivariate Biostatistics

Towards Integration of CFD and Photosynthetic Reaction Kinetics

in Modeling of Microalgae Culture Systems . . . . . . . . . . . . . . . . . . . . . . . . 679
Štěpán Papáček, Jiří Jablonský, and Karel Petera

Kinetic Modelling of Processes Behind S2;3 -states Deactivation

in Photosynthetic Oxygen Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 691
Jiri Jablonsky and Stepan Papacek

Observation of Dynamics Inside an Unlabeled Live Cell

Using a Bright-Field Photon Microscopy: Evaluation
of Organelles’ Trajectories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 700
Renata Rychtáriková and Dalibor Štys

Automatic Multiparameter Acquisition in Aquaponics Systems . . . . . . . . . . . 712

Antonín Bárta, Pavel Souček, Vladyslav Bozhynov, and Pavla Urbanová

High Definition Method for Imaging Bacteria in Microconfined

Environments on Solid Media. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 726
Cesar A. Hernandez, Natalia Lopez-Barbosa, Crhistian C. Segura,
and Johann F. Osma

Author Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 737

 Contents – Part I

Advances in Computational Intelligence for Critical Care

Health Monitoring System Based on Parallel-APPROX SVM . . . . . . . . . . . . 3

Fahmi Ben Rejab, Walid Ksiaâ, and Kaouther Nouira

Machine Learning for Critical Care: An Overview and a Sepsis Case Study . . . 15
Alfredo Vellido, Vicent Ribas, Carles Morales, Adolfo Ruiz Sanmartín,
and Juan Carlos Ruiz-Rodríguez

Bioinformatics for Healthcare and Diseases

A Meta-Review of Feature Selection Techniques in the Context

of Microarray Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Zahra Mungloo-Dilmohamud, Yasmina Jaufeerally-Fakim,
and Carlos Peña-Reyes

Immune Network Technology on the Basis of Random Forest Algorithm

for Computer-Aided Drug Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Galina Samigulina and Samigulina Zarina

H-RACER: Hybrid RACER to Correct Substitution, Insertion,

and Deletion Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Salma Gomaa, Nahla A. Belal, and Yasser El-Sonbaty

A Multi-sensor Approach for Biomimetic Control of a Robotic

Prosthetic Hand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Jeetinder Ghataurah, Diego Ferigo, Lukas-Karim Merhi,
Brittany Pousett, and Carlo Menon

Feature Selection in Multiple Linear Regression Problems

with Fewer Samples Than Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Paul Schmude

Colormetric Experiments on Aquatic Organisms . . . . . . . . . . . . . . . . . . . . . 96

Jan Urban

Predicting Comprehensive Drug-Drug Interactions for New Drugs

via Triple Matrix Factorization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Jian-Yu Shi, Hua Huang, Jia-Xin Li, Peng Lei, Yan-Ning Zhang,
and Siu-Ming Yiu
XXX Contents – Part I

Diagnosis of Auditory Pathologies with Hidden Markov Models. . . . . . . . . . 118

Lilia Lazli, Mounir Boukadoum, Mohamed-Tayeb Laskri,
and Otmane Aït-Mohamed

Biclustering Based on Collinear Patterns . . . . . . . . . . . . . . . . . . . . . . . . . . 134

Leon Bobrowski

A Data- and Model-Driven Analysis Reveals the Multi-omic Landscape

of Ageing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Elisabeth Yaneske and Claudio Angione

Experimental Investigation of Frequency Chaos Game Representation

for in Silico and Accurate Classification of Viral Pathogens
from Genomic Sequences. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Emmanuel Adetiba, Joke A. Badejo, Surendra Thakur,
Victor O. Matthews, Marion O. Adebiyi, and Ezekiel F. Adebiyi

Gamified Mobile Blood Donation Applications . . . . . . . . . . . . . . . . . . . . . . 165

Lamyae Sardi, Ali Idri, and José Luis Fernández-Alemán

Medical Entity Recognition and Negation Extraction: Assessment

of NegEx on Health Records in Spanish . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Sara Santiso, Arantza Casillas, Alicia Pérez, and Maite Oronoz

RISK: A Random Optimization Interactive System Based on Kernel

Learning for Predicting Breast Cancer Disease Progression. . . . . . . . . . . . . . 189
Fiorella Guadagni, Fabio Massimo Zanzotto, Noemi Scarpato,
Alessandro Rullo, Silvia Riondino, Patrizia Ferroni, and Mario Roselli

Revealing the Relationship Between Human Genome Regions

and Pathological Phenotypes Through Network Analysis . . . . . . . . . . . . . . . 197
Elena Rojano, Pedro Seoane, Anibal Bueno-Amoros,
James Richard Perkins, and Juan Antonio Garcia-Ranea

Variety Behavior in the Piece-Wise Linear Model

of the p53-Regulatory Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 208
Magdalena Ochab, Krzysztof Puszynski, Andrzej Swierniak,
and Jerzy Klamka

Multi-omic Data Integration Elucidates Synechococcus Adaptation

Mechanisms to Fluctuations in Light Intensity and Salinity . . . . . . . . . . . . . 220
Supreeta Vijayakumar and Claudio Angione

MASS Studio: A Novel Software Utility to Simplify LC-MS Analyses

of Large Sets of Samples for Metabolomics . . . . . . . . . . . . . . . . . . . . . . . . 230
Germán Martínez, Víctor González-Menéndez, Jesús Martín,
Fernando Reyes, Olga Genilloud, and José R. Tormo
Another random document with
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COMMENTS

If children are spoken to kindly about the condition of their hands

and several are sent at once to wash, they will take less offense. It will
seem then a matter of school-room practice rather than a personal
affront.
Drills or plays are often used to bring about habits of cleanliness.

ILLUSTRATION (FIRST GRADE)

Miss Barry organized her first grade room “Sanitary

into a “Sanitary Brigade.” Every one’s own Brigade”
ten fingers were the private soldiers over which each one’s face was
the captain. One child in each row, appointed by the teacher each
Monday morning, was the Lieutenant General of his row, and Miss
Barry was the Major General of them all.
Each captain inspected his ten soldiers and demanded that each
one—right and left Thumbkin, right and left Pointer, right and left
Longman, Ringman and Littleman—be perfectly clean. Then the
Colonel inspected the captains and finally the General had a grand
review of all the troops. The captains and colonels made daily
inspections. If they failed to do their work well they were sent to the
ranks and new officers appointed in their places. In other words, if
one did not keep his own hands clean, some one else was appointed
to be inspector and reporter of his hands. If any one was found with
unclean hands or face, a new colonel was appointed for the row in
which that child was found.
Inspection was made every morning and any reported disorder
was remedied in the lavatory. When there was no further need of
scrutiny as to clean hands and faces and when therefore the game
had lost in interest, a similar game was instituted that required clean
teeth, brushed hair, and clean shoes.

CASE 79 (THIRD GRADE)

 Miss Burr, the third grade teacher, sighed Use of
as Elma Colders passed her handkerchief to Handkerchief
her twin sister Zelma. This was a daily, almost hourly, occurrence.
The handkerchief wasn’t absolutely clean. These twins seemed to
take turns having colds, and Miss Burr believed the common
handkerchief was largely to blame for the transferred infection, but
she dared to say nothing about it.
Near to her desk sat Asa Kramer, who sniffed momentarily for
want of a handkerchief, while Fannie Black had a habit of often
wiping her nose with an upward stroke that was fast causing her nose
to have a decided skyward slant at the end.
One day she saw Morris Millspaugh repeat his habit of wiping his
nose on his coat sleeve and the following quotation popped into her
head, “Ye gods! Must I endure all this?” As if in answer to her
question, Annie Daily, in the back row, lowered her head and used
the under side of the bottom of her dress for a handkerchief.
“My query is answered,” groaned she. “Annie’s action says, ‘All
this? Ay, more!’”
Miss Burr said to a teacher friend, “If I were the family doctor of
these children, I’d be free to give their mothers a lecture on
sanitation, but since I’m not, I must keep within my prescribed
field.”

CONSTRUCTIVE TREATMENT

Correct these disgusting habits of your pupils. This can be done by

asking each child to bring a clean handkerchief every morning. A
certain teacher each morning asked all who received a grade of one
hundred in spelling or arithmetic the day before to stand and walk to
the front of the room. She then asked the other children who had
clean handkerchiefs to give them the Chautauqua salute, and the
children in front were asked to return this salute. The children
enjoyed this immensely and demanded from their mothers clean
handkerchiefs every morning in order that they might be allowed to
take part in this exercise.
COMMENTS

Miss Burr was wrong to conclude that it was not her business to
protect the health of her pupils.
There were several beneficial results accompanying the
Chautauqua salute program described above. Those who did good
work were rewarded and those who cheered them were given drill in
the hard task of praising their fellows who had succeeded where they
themselves had failed.
This salute was given in the morning, so that all handkerchiefs
would be clean for it.
Teachers can do no better than tactfully Enlist the
to enlist the aid of mothers in matters of Mothers
cleanliness.

ILLUSTRATION (FOURTH GRADE)

Miss Shaw, who taught the fourth grade in a very poor district in
New York City, organized a mothers’ meeting to convene alternate
Fridays after school hours. At one of these meetings she asked a
trained nurse to talk about cleanliness especially.
After the nurse had clearly explained the danger of infection in the
care of the nose, a mother who was a good shopper was delegated to
take orders for handkerchiefs from all present who needed them for
their children, to buy by the dozen and to deliver to the mothers at
cost.
By teaching and helping the mothers in this way, Miss Shaw
bettered conditions in her own room and established a wholesome
community spirit among her patrons.

CASE 80 (FIFTH GRADE)

Something was the matter with Dora Decaying Teeth

Payne. Miss Hubbart, her teacher, was
astonished at her stupid answers and generally inattentive attitude.
Usually Dora was alert and smiling; today she was morose, even to
tearfulness. Miss Hubbart finally said, “What is the matter, Dora?”
“My tooth aches,” said Dora.
“Did it ever ache before?”
“Yes, it ached last week and mamma took me to the dentist.”
“Charlotte, you may go with Dora to the dentist to see if he can
stop her toothache,” said Miss Hubbart.
The girls were gone only about half an hour, for it was but a few
blocks to the office of the only dentist in the village. After they came
back Dora was relieved and went on with her school work as usual.
When Miss Hubbart returned to the schoolhouse for the afternoon
session that day, she was greeted by Mr. Payne, Dora’s father, who
said:
“Dora says you sent her to Dr. Hammond’s office today.”
“Yes, she was suffering with toothache.”
“What I want to know is, who is going to pay the bill?”
“Surely you wouldn’t want Dora to suffer with toothache.”
“That tooth has troubled Dora before and my wife took her to Dr.
Hammond and he said that she’d lose this tooth in a year or so, and
we concluded not to have it worked on. When she gets her last set of
teeth it will not be money thrown away to have them filled.”
“But you wouldn’t want Dora to suffer for a year, surely.”
“Maybe we know how to take care of our own child without the
help of a stranger. I’ll thank you to keep on your own ground after
this.” And Mr. Payne stalked away with anger showing even in his
walk.

CONSTRUCTIVE TREATMENT

When you feel assured that, from a health standpoint, a child is

unfit to do school work, send him home and as soon as possible
thereafter consult with his mother as to his health, giving advice only
when you see that his parents are ignorant or neglectful.
In the matter of the care of the mouth and teeth, give instruction
as to the age a child should be when the several kinds of permanent
teeth appear. Name the causes of decay of teeth and show children
how to use their tooth brushes to the best advantage.

COMMENTS

Teachers should never send children to a Rhythmic Drills

doctor without the consent of their parents.
A teacher’s help can be rendered in instituting preventive measures
better than in administering or advising curative remedies. The harm
done to the teeth by allowing them to decay through the use of
improper food, also the proper use of the tooth brush, and the value
of a sterilizing mouth wash can easily be taught and come within the
teacher’s legitimate province. In many towns she may also secure the
coöperation of the school nurse.

ILLUSTRATION (FIFTH GRADE)

Miss Stow, who taught the fifth grade at Deadwood, made up this
motion song which the children sang occasionally, suiting the proper
motions to each verse:

This is the way we brush our teeth

At morning, noon and night,
Keeping them free from food and germs,
Making them clean and white.

This is the way we brush our hair,

Making it smooth and clean,
Keeping it free from kinks and dust
And beautiful to be seen.

This is the way we brush our shoes,

Making them fairly shine,
Then we ever shun dirt and mud
And keep them looking fine.
 When they sang the first verse she let them hold lead pencils in
front of their mouths for tooth brushes and had them make the up
and down motion that dentists recommend.
She noticed a marked improvement in the personal habits of her
children after they had learned this song, and they very much
enjoyed singing it.

CASE 81 (FOURTH GRADE)

Merrill MacFarland was elected to a Scattering Paper

position as teacher of the fourth grade after
three years of experience in the country schools. He had found the
circumstances in his former situation very unsatisfactory, and
resolved that since he was entering upon work in a graded school, he
would have some things different. Looking over the room after he
had wrestled his way through the first week, and recalling the events
of the past five days, he was strongly reminded of his former school
experience, since there was really a disgraceful amount of waste
paper all over the floor. The janitor had been complaining about the
matter, but MacFarland had been too busy with other matters to give
attention to it.
Monday morning, after the opening exercises, he made this
announcement:
“Now, I want every one of you boys and girls just the moment you
have a piece of waste paper in your hand to go to the wastebasket
and throw it in. Last Friday our room was a perfect disgrace. On
every desk there were slips of torn-up paper and some whole sheets.
We can’t get along this way. George, you just this moment dropped a
piece of paper in the aisle there at your left. Pick it up at once and put
it in the wastebasket as I just told you.” George noisily moved his
slow frame according to the order, hardly imagining what would be
the case if the teacher’s idea were literally carried out and no more
waste papers were thrown upon the floor.
As soon as he had reached the basket, Mr. MacFarland discovered
that there were several pieces of paper on the floor in different parts
of the room, and said, “Each one look about his desk now and see if
there is any rubbish on the floor that should be taken away. If so,
pick it up at once and throw it into the basket as I told you. This is
the thing to do at any time when you want to throw something
away.”
It need hardly be said that, with a great bustle, each one made the
desired search, and about nine pupils soon were on their way to the
basket with something or almost nothing that needed to be thrown
away.
However unsatisfactory the noisy method had proved, it did bring
about the condition desired, for at the end of the day there was really
nothing that needed to be complained of regarding the cleanliness of
the room, so far as waste paper was concerned.

CONSTRUCTIVE TREATMENT

A wise teacher does not make so much ado about one aspect of
school-room management. Mr. MacFarland could easily have
foreseen that he was introducing another evil along with his reform.
Reverse the process. Arrange that the wastebasket be passed around
mid-way between intermissions according to a definite schedule. Let
the passing of the basket be a privilege that is handed down from one
pupil to another according as they are seated, beginning with the
pupil who sits to the extreme right of the teacher’s desk. One pupil
each day is appointed as guardian of waste. The privilege and honor
will be appreciated, and with a word of caution, the service can be
performed with the very slightest interruption.
The following instructions might be properly given to the children
when the new system is established:
“We are going to save time as much as possible. Four times every
day we shall pass the wastebasket and you may put into it whatever
you have on hand to be thrown away. Nothing is to be said to the
person who carries the basket. He will go quietly, promptly, passing
down the aisles, doing a favor to everyone in the room by his careful
attention to this matter.”

COMMENTS
 It is of the utmost importance that the necessary organization of
school-room conduct be established according to principles and
policies that are useful to the child at home and in other situations
outside of school life. He must be taught how to economize his own
time, and the attention of his fellows, how to do necessary things in a
way that shall be both effective and unobtrusive.
While the handling of waste is a small affair for one room in a
school, taken on a larger scale for home, state and nation, the
problem of waste is big enough to command the attention of every
citizen; therefore, to dispose of the matter properly in school is a
valuable lesson in civics and economics.

ILLUSTRATION (FIFTH AND SIXTH GRADES)

Supt. Kennelworth suffered the inconvenience of moving from an

old building into the new one near the middle of a school year.
In the final plans for the proper use of the Bags for Paper
new plant, it had been agreed that each
pupil must bring with him a small waste-paper bag to be attached to
each desk. There were no very definite rules as to its size, but the
warning was given that it must not be too large for the convenience
of all concerned.
The matter was brought up only once, each teacher making the
announcement in his own room according to the superintendent’s
instructions.
Wallace Jackson made his announcement for the fifth and sixth
grades as follows: “Each one of you is to bring a small, neatly made
waste-paper bag, barely large enough to hold what you think is the
waste paper that gathers at your desk every day. Are there any
questions about this?” A hand went up and the question was asked,
“Who is going to empty these bags?”
“Well,” said Mr. Jackson, “you will empty them yourselves. Just
before the close of school each day the wastebasket will be passed;
your bags will be laid down upon your desk, and in just a very few
minutes every bag will be emptied and placed in your desk. Any more
questions?”
 Another hand and another question, “How are we going to decide
who will carry the basket?”
This was answered by the statement: “I shall appoint some pupil to
this task every day. The method of selection will be announced to you
later. The only matter I want now to tell you about is the making of
the bags. Further details will be given to you when we actually begin
our work next Thursday in the new building.” Owing to the fact that
some success in keeping a neat room had been attained already in
the old building, Mr. Jackson’s announcement was received without
surprise or anxiety.
(10) Imitation of wrong social standard. There is an imitation of
mere precedent which is a sort of social instinct, that can best be
handled socially. This is because the responsibility can be fixed on no
one individual, and as all the members of the group are equally
guilty, all should share in the needed lesson.

CASE 82 (RURAL SCHOOL)

George Marston went into the country as Dropping Shot

teacher in a school in which there had been
much bad order. The directors were in earnest; they wanted a good
school, but for the salary they offered they had not been able to
secure a man who could handle the big boys who made the trouble.
They were not especially bad boys; but the tradition of mischief in
the school was so persistent that no teacher had been able to
overcome it.
During the fall term there was no trouble, for then only meek little
girls and small boys attended. But on the first Monday after
Thanksgiving, corn-shucking being over, the older pupils came in an
avalanche of good-natured noise. The room, before so sparsely
populated by a few pupils, seemed to overflow with their energy.
Trouble was not long in coming, and it took the shape of a shower of
fine shot, which pattered down from the ceiling during the spelling
lesson. George knew that the hour of trial had come, and he called
out bravely:
“Who did that?”
 There was no response. The little girls were peeping timidly from
their books, but the big boys and girls frankly relished the coming
fun.
“We may as well settle it now,” said George Marston. “We can’t
have a school without good order. Of course I want to be just, and
first of all I want to know who threw that shot. Will you tell me?”
No one spoke. Then the teacher went around the room, asking
each one in turn if he had done it. Every one denied it. There was
clearly an understanding among the pupils that gave the teacher no
chance.
So Marston gave it up for the time being, and lessons were taken
up again. But at four o’clock he asked four of the leading boys, those
he suspected most, to stay after school. When the rest had gone, he
conducted an exhaustive examination, trying to find who was
responsible for the disturbance. The evidence was flippant,
contradictory, mockingly frank; but he found out nothing. Still with
his idea of locating the offense in one person, George held another
trial the next night, with the same result. Then he took the matter to
the board.
“One or two persons must have thrown that shot,” he said to the
board members, “and if I can find the person who did it, and make
an example of him, then I know I can manage the school without
trouble. But so far I can’t find the offender. There doesn’t seem to be
a ringleader; they all hang together so.”
“Why can’t you find the offender?” asked one member.
“Because they all lie about it—at least there’s one person who is
lying. If only I could find that one person!”
“Why not lick them all, since they’re all mixed up in it?” This
director was a coarsely practical man.
“Because one person did the deed, and he ought to bear the
punishment,” George replied. “If I keep my eyes open and wait, in
time I’ll be sure to catch the boy that threw that shot.”
So he waited and kept his eyes open, but he never discovered the
shot-thrower. There was much more misbehavior during the days
that followed, and the struggle to keep even a semblance of order
made the term a nightmare to the harassed teacher. Little real work
was accomplished. The board, anxious to have a good school, but
ignorant of principles and methods, saw its desire come to nothing.
Marston’s ideas of discipline seemed to be centered on “making an
example” of some one offender, and the school took a mischievous
satisfaction in shielding each offender from discovery.
The crisis came late in January. Marston Explosion
had just put a full scuttle of coal on the
blazing fire in the big stove, when a sharp noise and a great puff of
smoke and flame burst from it. The explosion broke apart the
sections of the stove, and a serious fire was averted with some
difficulty. When they had made things safe and could look at each
other with smoke-grimed faces, teacher and pupils knew that a
reckoning was at hand.
“John Coffey, you brought in that coal. Did you put the gunpowder
in it?” John was fairly cool, and the reckless boys had been cowed
and sobered by the extent of the mischief that had been done.
“No, sir, I didn’t,” replied John; and his denial rang true.
“Did you, Carl?”
“No, sir.”
Marston went around the circle, as he had many times before; and
all denied their guilt. Finally Marston turned to the school.
“You may all go home now. We can’t have school until this mess is
cleaned up, of course. I shall see the board at once.”
The board decided, at a called meeting, that it would best get a new
teacher, and Marston resigned with infinite relief. The board sent to
a state normal school, explained the situation, and asked for a strong
man.

ILLUSTRATION (RURAL SCHOOL)

The president of the normal school sent them Isidor Thomberg, a

man of experience and high scholarship. He came with the
understanding that the board was to support whatever he did, and he
agreed to reduce the school to order. He was confident, fearless, and
told no one of his plans. But he did meet the board on the night of his
arrival, and heard their full account of the troubles.
 The next morning school opened as usual, with the new teacher
and the new stove dividing the honors of attention. Mr. Thomberg
made just one reference to the situation:
“Of course you know why I’m here,” he said. “I want to say one
thing. I shall never waste a moment’s time trying to find out who
does anything bad. We have to make up for a great deal of lost time
this winter; you’ll have to work hard from now until spring. We shall
have no school but a good one. Now we’ll go to work.”
Under the stimulus of his quiet confidence, the order was excellent
that first day. The school needed organization, and much time was
spent in showing the pupils economical ways of doing things. While
the novelty lasted there was no tendency to disorder, and when
things settled down into a regular routine the lessons proved so
interesting under Mr. Thomberg’s teaching that the boys forgot for a
time to have fun in the old way. A fancy skating club had been
organized; a new era seemed to have dawned, when one day, quite
unexpectedly, the old problem popped up again.
A row of pupils, coming quietly forward to the recitation bench,
found themselves stepping on a number of match-heads which had
been scattered in the aisle. At the same moment Mr. Thomberg
himself, who had stepped to a window to adjust a shade, exploded
two of these little trouble-makers. Every one looked up in surprise,
for bad order had been almost forgotten.
The teacher went to his desk. Very quietly he sent the class back to
their seats. Then he told the pupils to put away all books, and they
obeyed in a dazed way, afraid of they knew not what.
“I told you when I came,” said Mr. Thomberg, “that we couldn’t
have anything but a good school. The reason that we can’t afford to
have bad order is that we have too much serious work to do. I had
begun to like you all so much that I’m sorry some one has had to
spoil our pleasant beginning. But I meant what I said. So this school
is closed, now, indefinitely. You are all, without exception, suspended
from school until further notice. You will pass out as you always do.”
There was a breathless silence. Such a thing as suspending a whole
school had never been heard of before. Mr. Thomberg gave the usual
signals, and the boys and girls passed into the hall as though they
had been at a funeral. Mr. Thomberg went to the nearest house and
called the directors by telephone for a conference, which was scarcely
begun when parents began to inquire indignantly why their children,
guiltless of dropping match-heads, had been suspended from school.
Mr. Thomberg dictated the answer:
“Tell them,” he said, “that I have no time to ferret out the doers of
silly little tricks in my school. When the school gives me the
assurance that there’ll be no more trouble, then we’ll go back to
work. Whoever dropped those match-heads thought the school liked
that sort of thing, and you must show him that he is mistaken. I am a
teacher, not a policeman.”
The parents really wanted their children in school, and guided by
the suggestion, skillfully made by the teacher, they took steps to
secure the concerted action which Mr. Thomberg knew was the
remedy for the evil. He was reading the daily paper in the living
room of his boarding house that evening, when the response for
which he had planned came. There were seventeen of his twenty-six
pupils in the party which called on him. One of the older boys, Felix
Curry, was spokesman.
“We came to ask you if you’d have us in school again,” he said. “All
our fathers and mothers want us to go back, and we’ll be good if
you’ll let us. The boy that threw the match-heads will tell you about it
himself. He told us he would.”
“There is no need of that, although he may do so privately
tomorrow if he wants to. But I should rather not know who did it, if
you’ll all be responsible for its not happening again. You see, I like
you all so much that I’d hate to know who did so foolish and wrong a
thing. And if you will agree that it is to be a good school, in which
everyone works together in the right spirit, I’ll agree to stay until the
end of the year. Otherwise I pack my trunk tonight.”
The pupils gave ample promises, which were not broken. Mr.
Thomberg stayed through that year and the next one also, and had
the best school in the county.

COMMENTS

This is an extraordinarily difficult situation. Mr. Thomberg’s

success in dealing with this unusual case depended first of all on the
fact that his knowledge of pedagogy enabled him to analyze the
situation truly. He saw that the bad order was not caused by any one
pupil, but was the result of a social tradition for which many persons,
in and out of the school, were responsible. All the pupils upheld the
disorder; therefore all the pupils were dealt with in a group.
In the second place he was independent, as a really first-class
teacher can be. He set the standard of behavior, and required his
students to come up to it or give up school altogether. Had the school
declined to take the social responsibility he asked of them, he would
really have packed his trunk and left. He was well prepared for his
work, and was greatly in earnest about it, nor did he propose to do
what he considered an undignified thing in probing for evil.
His personality was strong enough to set up a new standard for
imitation, and to supplant the old one of inefficiency and mischief.
The problem of order was for a time swallowed up in the greater one
of securing real mental development in his pupils, and when it did
show itself he treated it as a matter to be dealt with socially by the
pupils. This seemed to put upon them a responsibility they had been
used to having the teacher assume unaided, and they rose to the new
honors imposed upon them. Mr. Thomberg utilized imitation in
setting up a new regime, by requiring united action of his pupils. In
this way he met a psychological situation with psychological
weapons.
(11) Snobbishness. Snobbishness is a very hard thing to meet
wisely, and a sin which is too easily learned by imitation.

CASE 83 (HIGH SCHOOL)

Joseph Lescinszky was a tailor in a small Middle West city, who

lived in five small rooms over his shop, with his wife and seven
children. He had plenty of patronage, for he was a good tailor, but he
was unhappy, for one of the dreams long associated with his
American citizenship had failed to materialize. His eldest son,
Joseph Junior, was in the high school—a homely, awkward boy with
great wistful eyes and an incurable shyness. He was a good student,
but suffered constantly under the heartless, matter-of-fact ridicule of
his American schoolmates.
 This ridicule was but the echo of the Race Prejudice
attitude of the whole community toward the
Polish family. They were the only foreigners in the place, and their
appearance, habits and speech afforded unlimited amusement to
everyone. Men who had had their suits made by the skillful, little,
Polish tailor told funny stories in which his broken English figured as
the chief point, and their sons and daughters in turn laughed at the
shy and awkward children whom they met at school.
No one realized how this ridicule embittered the life of the tailor
until Miss Swainson came to Hovey to teach in the high school. She
took her old cape to the tailor one day, thriftily planning to have it
made into a coat for school wear; and over the making of the coat,
tailor and teacher began to discuss Joseph and his school life. No
teacher had ever talked to him about Joseph before, and the little
Pole voiced his feelings tremblingly.
“My Joseph does not like the school,” he confided. “He goes
because I command him that he shall, but he has not his heart in it.”
“He does very good work,” comforted Miss Swainson. “His lessons
are always excellent.”
“Ah, the lessons he gets with no trouble. But the boys, they like
him not. They all time make fun on him, and my poor Joseph is not
happy. He is Polack, they say. It is not true—we are Americans now;
there is the paper,” and he pointed proudly to the naturalization
paper, framed upon the wall.
“We’ll see if we can’t make Joseph happy at school,” Miss
Swainson promised. “I’ll see what I can do.”
Now, Miss Swainson was one of the upright, downright people
who go at things directly and openly. She began a campaign for the
kinder treatment of Joseph Lescinszky, Junior. She had no doubt of
her success.
An opportunity for beginning her kindly efforts in Joseph’s behalf
came soon. Joseph was always the butt for practical jokes, and
Charlie Owen was his particular tormentor. Therefore, when Joseph
was tripped, going to the dictionary, by Charlie’s projecting toes, she
kept Charlie after school and had a serious talk with him.
“Don’t you see what a contemptible thing it is for you to tease this
bright boy just because he is of Polish blood?” she inquired warmly
of smiling Charlie Owen.
“Why, Miss Swainson, he don’t mind. Everybody always laughs at
those Lescinszkies, and they don’t care. If they ever showed fight like
Americans I guess we’d let them alone, but they don’t. They’re not
like us.”
“That’s just why you should treat them better. They haven’t
learned that a boy has to fight in order to be decently treated,” Miss
Swainson returned with fine scorn.
“No, ma’am. If they could only learn that, they’d be all right.” Fine
scorn was wasted on Charlie.
All Miss Swainson’s efforts ended in this way. Secure in their
feeling that traditional American means for securing respect were the
only ones, smug with the provincialism of the small town, the school
children continued to express to the alien boy the contempt they had
imbibed from their elders. They merely smiled at Miss Swainson’s
indignant efforts to win justice and kindness for their Polish
schoolmate.
Toward the end of the year the young teacher thought she detected
a shade of difference in the treatment given Joseph. This was due
partly to her constant shaming of the thoughtless cruelty of their
conduct, and partly to the respect he himself won by his good work in
the classroom. But although this small degree of success comforted
her somewhat, she felt still the defeat of her efforts keenly, not
realizing that a change of conduct based on imitation must come
through a change of example followed.

COMMENTS

Miss Swainson’s method did not go deep enough. Mere protest will
occasionally effect a change, but not often. If she could have gotten
behind Charlie Owen’s attitude, and found its roots, which were
probably in the attitude of some member of his family or a powerful
older friend, she might have truly converted Charlie to her way of
thinking. His statement that “everybody always laughs at those
Lescinszkies,” might have given her a clue, but she did not realize
that she was only working on the surface.
ILLUSTRATION (RURAL SCHOOL)

Sallie Lou Pinkston came from Mobile, Alabama, to the small,

northern town of Cade Mills, when she was ten years old. She was
the only girl in an adoring family of brothers—a lovely, sunny-haired
child, with the confidence in her right to rule her world which her
happy family life had given her. She entered the fifth grade that fall,
and scored an immediate success with the whole room, teacher
included. Her southern accent was a continual source of delight; her
matter-of-course assumption that everyone wanted to do the
entertaining things she planned kept the whole room, with two
exceptions, tied to her dainty apron-strings.
The two exceptions were Laurastine and Enameline Flack.
Laurastine and Enameline belonged to the one colored family in
town, and they had always attended the public school and had always
been well treated by the other children. But when Sallie Lou cast her
golden charm over the room, things changed. Sallie Lou didn’t
understand why they should be there at all, and she surely didn’t
intend to tolerate them as her social equals. Led by the irresistible
charm of Sallie Lou, vying with each other to stand in her graces, the
other children began to ignore the two little colored girls, or openly
to laugh at them and pointedly to leave them out of their games.
Miss Stone, the teacher, being a northern woman and a believer in
literal democracy, thought this a very bad state of things indeed. The
two little girls were well behaved, wistful, little creatures, whose tears
at the new turn of things went straight to Miss Stone’s big heart. She
knew that the remedy must come in some way through Sallie Lou,
who had caused the havoc; for there was no possible way to supplant
her dominant influence, nor was such a thing desirable except for
this one cause. Sallie Lou had wakened the room to a new interest in
many things, and in everything except the treatment of the little
“niggers” she was sweetness and docility itself.
Could Sallie Lou be converted? Miss Stone tried it, with doubt in
her heart; she knew what prejudice is. She invited Sallie Lou to her
house for supper one Friday night, and after supper they sat by the
fire and had a long talk about it. Miss Stone presented the pathetic
cases of Laurastine and Enameline as touchingly as she could, but
Sallie Lou merely smiled divinely and told her, most sweetly, that of
course she wanted to do what was right, but that it wasn’t right for
“niggers” to mix socially with white people, and that the town should
provide a separate school for them. She was firmly entrenched
behind the prejudice of her rearing in a community which solved the
problem this way.
Miss Stone reluctantly retreated from the attack, but she did not
give up. She went behind the prejudice to its support, behind the
example to its example. She cultivated Sallie Lou’s mother, her
father, her four charming brothers. The parents, finding few cultured
people in the little town, welcomed the well-read teacher and were
very cordial to her. When she had won their respect and liking, Miss
Stone asked for a frank talk with them about the conditions in her
room, and told them just how the irresistible Sallie Lou was leading
all the other children in the fifth grade to snub the two little Flacks.
At first the Pinkstons were amused, then they were indignant.
They too felt that the two colored children had no business in a
school for white children. But when Miss Stone had shown them that
a separate school was impracticable in a town that had but one
colored family, that conditions were very different from those of a
southern city, and that a change in Sallie Lou’s attitude would avert a
personal tragedy for two innocent little girls, the parents came finally
to see the matter from her point of view. They promised to talk to
Sallie Lou and to persuade her to change her tactics. Miss Stone
thanked them, and changed the subject quickly and brightly to
something else.
Sallie Lou, at first with formal and awkward condescension, but
later with the same frank charm that won everyone to her, asked the
two little outcasts back into the fold of fifth grade fun. The rest fell
easily into their old democratic way of sharing things. Miss Stone
had solved the problem of race prejudice by changing the example.
2. Play—A Second Form of the Adaptive Instincts
The child never reveals his whole nature as he does when playing. His physical,
mental and moral powers are all called then into vigorous exercise. On the
playground the boy begins to learn how to struggle with his fellow men in the great
battle of life. His strength and his weakness both manifest themselves there, so
that it pays to study him.

—Hughes.
 Much school trouble is caused by the purely sportive impulses of
childhood, impulses which are in themselves entirely innocent and
wholesome. One of the most valuable parts of a child’s training is the
acquiring of a set of notions as to appropriateness—the knowledge of
when he may, and when he may not, rightly give rein to his wish to
play. Some children acquire these ideas of propriety readily, and
adapt themselves seemingly without effort to the customs of their
environment; but most children stumble through the period of
adaptation with many backslidings, for the instinct of play is stronger
than the instinct of adaptation to requirement.
But let it be remembered meanwhile that this same play instinct is
one of the strongest allies of the teacher in securing such adaptation,
if the instinct is properly directed. What lesson of politeness,
neatness, unselfishness, protection of the weak, promptness,
responsibility, care of pets, coöperation, chivalry—yes, even of duty
and religion—may not be taught through play! Draining off the play
impulses into these legitimate channels will relieve many a
wearisome, perplexing day for both teacher and pupils, and at the
same time speed on the child toward conscious self-control.
Perhaps the greatest single help in teaching children the voluntary
limitation of their play impulses, is the knowledge that play is only
postponed, not forbidden. Most children have so strong a love of
approbation that they like to do things in the proper way if the
sacrifice be not too great. They are willing to put off their fun, but not
to put it off forever. The teacher who says, “If you’ll wait until recess,
I’ll show you how to play a new game with marbles,” will secure
willing obedience, when she who takes the marbles away has only
sullen submission for her reward. Here the teacher utilizes the
instinctive love of novelty in teaching control of play impulses.
During the period, when conduct is so largely a matter of instinct,
wise teachers play off one instinct against another to the child’s gain,
knowing that some impulses need encouragement and others need to
be inhibited.
(1) “Just mischief.” One of the most First Grade
frequent ways in which the play instinct
expresses itself in the first and even in higher grades is in the little
annoyances which teachers group together under the general term,
mischief. An energetic child, if he is not constantly employed,
naturally vents his energy either in play or in trying to satisfy his
curiosity.
The principle of suggestion alone will often be sufficient to control
the child. The principle of substitution will work equally well.
Coöperation can be correlated with either of these, and expectation
that the child will do what the teacher desires should be used in
whatever method the teacher may adopt.
The mischievous boy will be quiet so long as he is reciting, but
while others are reciting he will immediately hunt for something else
to do. He will drag his shoe on the floor, reach over and touch his
neighbor, pull out a pocketful of string or help himself to his
neighbor’s pencil.
It is not a case for punishment, but a time to apply one or more of
the fundamental principles. The teacher, without even a word or
look, may reach over and draw him to her side, asking him to look on
her book, or better still she may look on his book (suggesting that he
attend to his lesson). She may send him on some little errand—to
bring a book or a piece of crayon—and by the time she whispers,
“Thank you, you did that like a little gentleman,” he will have
forgotten all about his mischief (substitution and approval).
When the class is dismissed, however, the teacher must see that
the mischievous boy is kept busy and his work changed once or twice
within the half hour. She must not fail to show an interest in his
work. The comment, “That is so good, my boy, that I want you to put
it on my desk where we can look at it,” will so elate the child that he
will work industriously to do his best, and doing his best will keep
him busy a long time. When on the playground or in the gymnasium
the teacher must see that he gets plenty of “full-of-fun” play. It will
use up some of his restless energy.
The teacher must have an abundance of busy work ready for the
next restless period that comes, sorting blocks or marbles, straws or
papers, cutting or coloring pictures, putting books on the shelf, and
anything that will keep him innocently busy. Stencil cards, cutting
out words or letters, the distributing of materials for the class to use,
games for the recess and noon periods, frequent story periods during
school hours, interesting lessons, vigorous exercise, generous
approval of every effort to please the teacher—all these will gradually
win the mischievous boy to habits of self-control and industry. All