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    3 views5 pages

    UV Detector Linearity & Sensitivity Report

    Uploaded by

    Riad Manamanni

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    UV DETECTOR LINEARITY& SENSITIVITYREPORT

    ACQUI SI TI ON I NFORMATI ON

    SystemName: Iclass_BSM_FTN_TUV_ELSD_S3 Acquired By: Natalia


    Project Name: SQT_E_BSM_FTN_TUV_ELSD Column serial Number: 017139167157 42
    Sample Set Method: 04b Detector Lin SSM Sample Kit 1 Expiration date: 1/25/2012 12:00:00 AM EST
    Processing Method Detector Area ELSD PM, Detector Area PM, Sample Kit 1 Lot Number: W04040902
    Report Method Name: Detector Lin RM Build Version: Empower 2 Software Build 2154 SPs Installed:
    User Name: Lisa Busher (lbusher) Service Pack
    Sample Set Id:G6649
    Hotfix 2 DB ID: 878755939
    Result Set Id 6660
    Sample Set Name: Detector Lin SSM Sample Set Start Date: 11/30/2011 8:36:35 PM EST

    The Detector Linearity &Sensitivity qualification injects several amounts of Caffeine and plots the Response vs.
    Caffeine Amount.
    It also computes the Sensitivity (PeakArea/Amount) and (Peak Height/Amount) for each injection.

    The qualification passes if the Linearity R^2 value for Area is > or = 0.9990 and the R^2 value for Height
    is > or = 0.990. The %RSD of the Sensitivity must be < or = 3.5%for Area and < or = 4.5%for Height.

    Detector Area Linearity Detector Height Linearity


    Height Linearity Height Linearity
    Area Linearity Area Linearity Height Linearity
    Area Linearity Curve R2 Curve R2
    Curve R2 Curve R2 Curve R2
    Curve R2 Specification Pass Fail
    Specification Pass Fail
    1 R^2 > or = 0.990 1.000 PASS
    1 R^2 > or = 0.9990 0.9999 PASS

    Detector Area Sensitivity Detector Area Sensitivity


    Area Area Height Height
    Area Height
    Sensitivity%RSD Sensitivity%RSD Sensitivity%RSD Sensitivity%RSD
    Sensitivity%RSD Sensitivity%RSD
    Specification Pass Fail Specification Pass Fail
    1 %RSD < or = 3.5 0.7 PASS 1 %RSD < or = 4.5 1.2 PASS

    Printed 6:27:15 PM US/Eastern 1/16/2012 Page: 1 of 5


    UV DETECTOR LINEARITY& SENSITIVITYREPORT

    ACQUI SI TI ON I NFORMATI ON

    SystemName: Iclass_BSM_FTN_TUV_ELSD_S3 Acquired By: Natalia


    Project Name: SQT_E_BSM_FTN_TUV_ELSD Column serial Number: 017139167157 42
    Sample Set Method: 04b Detector Lin SSM Sample Kit 1 Expiration date: 1/25/2012 12:00:00 AM EST
    Processing Method Detector Area ELSD PM, Detector Area PM, Sample Kit 1 Lot Number: W04040902
    Report Method Name: Detector Lin RM Build Version: Empower 2 Software Build 2154 SPs Installed:
    User Name: Lisa Busher (lbusher) Service Pack
    Sample Set Id:G6649
    Hotfix 2 DB ID: 878755939
    Result Set Id 6660
    Sample Set Name: Detector Lin SSM Sample Set Start Date: 11/30/2011 8:36:35 PM EST

    Detector Area Linearity Results


    Calibration Id 6721 Y Intercept 11163.873532

    Date Calibrated 11/30/2011 8:49:06 PM EST Slope 14467069.512081

    Fit Type Linear (1st Order) R^2 0.99988

    Calibration Plot
    Area vs. Amount

    2.5x106

    2.0x106

    1.5x106
    Area

    1.0x106

    5.0x105

    0.0
    0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16
    Amount
    Area R^2: 0.99988

    Printed 6:27:15 PM US/Eastern 1/16/2012 Page: 2 of 5


    UV DETECTOR LINEARITY& SENSITIVITYREPORT

    ACQUI SI TI ON I NFORMATI ON

    SystemName: Iclass_BSM_FTN_TUV_ELSD_S3 Acquired By: Natalia


    Project Name: SQT_E_BSM_FTN_TUV_ELSD Column serial Number: 017139167157 42
    Sample Set Method: 04b Detector Lin SSM Sample Kit 1 Expiration date: 1/25/2012 12:00:00 AM EST
    Processing Method Detector Area ELSD PM, Detector Area PM, Sample Kit 1 Lot Number: W04040902
    Report Method Name: Detector Lin RM Build Version: Empower 2 Software Build 2154 SPs Installed:
    User Name: Lisa Busher (lbusher) Service Pack
    Sample Set Id:G6649
    Hotfix 2 DB ID: 878755939
    Result Set Id 6660
    Sample Set Name: Detector Lin SSM Sample Set Start Date: 11/30/2011 8:36:35 PM EST

    Detector Height Linearity Results


    Calibration Id 6730 Y Intercept 19395.880119

    Date Calibrated 11/30/2011 8:49:14 PM EST Slope 14586255.263494

    Fit Type Linear (1st Order) R^2 0.9997

    Calibration Plot
    Height vs. Amount

    2.5x106

    2.0x106
    Height

    1.5x106

    1.0x106

    5.0x105

    0.0
    0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16
    Amount
    Height R^2: 0.9997

    Printed 6:27:15 PM US/Eastern 1/16/2012 Page: 3 of 5


    UV DETECTOR LINEARITY& SENSITIVITYREPORT

    ACQUI SI TI ON I NFORMATI ON

    SystemName: Iclass_BSM_FTN_TUV_ELSD_S3 Acquired By: Natalia


    Project Name: SQT_E_BSM_FTN_TUV_ELSD Column serial Number: 017139167157 42
    Sample Set Method: 04b Detector Lin SSM Sample Kit 1 Expiration date: 1/25/2012 12:00:00 AM EST
    Processing Method Detector Area ELSD PM, Detector Area PM, Sample Kit 1 Lot Number: W04040902
    Report Method Name: Detector Lin RM Build Version: Empower 2 Software Build 2154 SPs Installed:
    User Name: Lisa Busher (lbusher) Service Pack
    Sample Set Id:G6649
    Hotfix 2 DB ID: 878755939
    Result Set Id 6660
    Sample Set Name: Detector Lin SSM Sample Set Start Date: 11/30/2011 8:36:35 PM EST

    Caffeine Detector Linearity Overlaid Chromatogram


    400.00

    350.00

    300.00

    250.00

    200.00
    AU

    150.00

    100.00

    50.00

    0.00

    0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00
    Minutes

    Printed 6:27:15 PM US/Eastern 1/16/2012 Page: 4 of 5


    UV DETECTOR LINEARITY& SENSITIVITYREPORT

    ACQUI SI TI ON I NFORMATI ON

    SystemName: Iclass_BSM_FTN_TUV_ELSD_S3 Acquired By: Natalia


    Project Name: SQT_E_BSM_FTN_TUV_ELSD Column serial Number: 017139167157 42
    Sample Set Method: 04b Detector Lin SSM Sample Kit 1 Expiration date: 1/25/2012 12:00:00 AM EST
    Processing Method Detector Area ELSD PM, Detector Area PM, Sample Kit 1 Lot Number: W04040902
    Report Method Name: Detector Lin RM Build Version: Empower 2 Software Build 2154 SPs Installed:
    User Name: Lisa Busher (lbusher) Service Pack
    Sample Set Id:G6649
    Hotfix 2 DB ID: 878755939
    Result Set Id 6660
    Sample Set Name: Detector Lin SSM Sample Set Start Date: 11/30/2011 8:36:35 PM EST

    Detector Area Sensitivity Results


    Peak: Caffeine
    Area
    Vial SampleName Level Amount Area_Sensitivity Result Id
    (µV*sec)
    1 2:F,1 DetLin_Resp_1 1 147972 0.01 14797247.69 6723
    2 2:F,2 DetLin_Resp_2 2 441658 0.03 14721927.28 6724
    3 2:F,3 DetLin_Resp_3 3 590031 0.04 14750763.22 6725
    4 2:F,4 DetLin_Resp_4 4 736989 0.05 14739771.64 6726
    5 2:F,5 DetLin_Resp_5 5 1177921 0.08 14724007.66 6727
    6 2:F,6 DetLin_Resp_6 6 1467729 0.10 14677287.78 6728
    7 2:F,7 DetLin_Resp_7 7 2315371 0.16 14471068.68 6729
    Mean 14697439.13
    Std. Dev. 106107.23
    % RSD 0.72

    Detector Height Sensitivity Results


    Peak: Caffeine
    Height
    Vial SampleName Level Amount Height_Sensitivity Result Id
    (µV)
    1 2:F,1 DetLin_Resp_1 1 151311 0.010 15131093.83 6732
    2 2:F,2 DetLin_Resp_2 2 451857 0.030 15061908.60 6733
    3 2:F,3 DetLin_Resp_3 3 602837 0.040 15070925.23 6734
    4 2:F,4 DetLin_Resp_4 4 751514 0.050 15030289.91 6735
    5 2:F,5 DetLin_Resp_5 5 1206136 0.080 15076703.61 6736
    6 2:F,6 DetLin_Resp_6 6 1492418 0.100 14924178.67 6737
    7 2:F,7 DetLin_Resp_7 7 2335237 0.160 14595232.99 6738
    Mean 14984333.26
    Std. Dev. 182901.00
    % RSD 1.22

    Printed 6:27:15 PM US/Eastern 1/16/2012 Page: 5 of 5

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