115

23 2 (2550)

X
(Dendrobium Green Lantern)
*

X
(Dendrobium Green lantern) 9 1.5-2.0
Vacin and Went (VW) (1949)
N6-furfuryladenine (Kinetin) N6-benzylaminopurine (BA)
Thidiazuron (TDZ) N6isopentenyladenine (2iP) Zeatin 0, 0.1, 0.5,
1.0, 2.5 5.0 10 Zeatin 5.0
3.6
Zeatin 2.5
3.5 2.8 2iP 0.1
Zeatin 0.5
4.1 2iP 0.1
6.8
89.6% 8

:

*, e-mail: anupank73@hotmail.com

116

SWU Sci. J. Vol. 23 No. 2 (2007)

Effect of Cytokinins on Growth and

Development of Young Shoots of
Dendrobium Hybrid (Dendrobium Green
Lantern)
Anupan Kongbangkerd* and Thanakorn Wongsa

ABSTRACT

In vitro shoot culture of Dendrobium hybrid (Dendrobium Green Lantern)
was investigated. Nine month olds of young shoots with 1.5-2 cm. in length were
used as starting explants. Explants were cultured on semi-solid Vacin and Went
(VW) (1949) medium supplemented with various types and concentrations of
cytokinins; N6-furfuryladenine (Kinetin), N6-benzylaminopurine (BA), Thidiazuron
(TDZ), N6isopentenyladenine (2iP) and Zeatin at 0, 0.1, 0.5, 1.0, 2.5 and 5.0 mg/l
for 10 weeks. The results shown that highest number of shoot regeneration (3.6
shoot per explants) was observed on the medium with 5.0 mg/l Zeatin whereas
highest shoot length (3.54 cm in length) and root length (2.81 cm in length) were
obtained when cultured on medium with 2.5 mg/l Zeatin. Adding either 0.1 mg/l
of 2iP or 0.5 mg/l of Zeatin to the medium could promote better leaf formation
(4.1 leaves per new shoot) whereas the medium with 0.1 mg/l of 2iP induced
highest root formation (6.8 roots per shoot). New regenerated plantlets were
succeeded to acclimatize in the greenhouse with 89.6% of survival.

Keywords: cytokinins, Dendrobium hybrids, in vitro culture, acclimatize in the
greenhouse

Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University
*Corresponding author, e-mail: anupank73@hotmail.com

 23 2 (2550)

117

30,000
x (Dendrobium Green Lantern)

[1]
(Dendrobium Dawn Maree)
Dendrobium Green Lantern




(mycorrhiza) [2]

[3-5]

[6] Zeatin 6isopentenyladenine (2iP)
benzyladenine (BA), 6furfurylaminopurine (Kinetin) phenylureas Thidiazuron (TDZ) [7-8]
protocormlike bodies (PLBs) [9]
Cattleya [10-11]

x (Dendrobium Green Lantern)

118

SWU Sci. J. Vol. 23 No. 2 (2007)

Dendrobium Green Lantern 1.5-2.0
3-4 ( 1) 9
VW (1949) [12] 5 0.02
5 Kinetin BA
TDZ 2iP Zeatin 6 0, 0.1, 0.5, 1.0, 2.5 5.0
40 mol.m-2.s-1
12 25 10
complete randomized design (CRD) 30


(ANOVA) Duncans new multiple
range test (DMRT) 95

2 1
2.5 2 2.5


8

5
Kinetin, BA, TDZ, 2iP Zeatin 10
Dendrobium
Green Lantern [ 1 2 (-)]
BA 2iP

 23 2 (2550)

119

Zeatin, Kinetin TDZ

2iP 0.1
4.1

VW Zeatin 5.0
3.6
Nayak (2002)
[13] Zeatin PLBs
Cymbidium aloifolium 89 VW
Zeatin 14.0




5
Zeatin 2.5
3.5
Coleman Ernst (1990) [14] Populus
deltoides Zeatin
Nayak (1997) [15]
TDZ 2.2-4.5 NAA 5.4
Cymbidium aloifolium (L.)
Dendrobium aphyllum (Roxb.) Fisch. Dendrobium moschatum (Buch-Ham) Sw.
Zeatin
Park (2002, 2003) [16, 17] TDZ

organogenesis embryogenesis Zeatin
Park 2002 [16] TDZ Zeatin
(thin cross section) Doritaenopsis hybrid
Murashige and Skoog (MS) (1962) TDZ 9.0
PLBs
Park 2003 [17]
Doritaenopsis MS TDZ 2.3 PLBs
47.2 Zeatin

[13]

120

SWU Sci. J. Vol. 23 No. 2 (2007)

2iP 0.1
6.8
(2542) [18]
PLBs VW BA / 2iP
0.5

VW
[19, 20] Dendrobium [21]

[22]
VW Zeatin 2.5

2.9

1
Dendrobium Green Lantern 10

(mg/l) (Mean SE)


(Mean SE) (Mean SE)**
(Mean

No
0.0 3.00.27bc* 1.80.21b 2.30.25def
hormone
0.1 3.60.14abc 1.60.15b 2.60.20bcdef
0.5 3.50.29abc 1.40.12b 3.00.28abcde
Kinetin 1.0 3.20.22abc 1.50.19b 3.00.29abcde
2.5 3.50.27abc 1.80.24b 3.00.24abcde

BA

SE) (Mean SE)**

3.00.34g

1.50.16def

4.30.38cdefg 1.50.10def
5.00.54abcdefg 1.60.01cdef
5.20.61abcdef 1.50.11def
4.40.41bcdefg 1.80.16bcdef

5.0 3.30.22abc 1.60.22b 2.20.25ef

3.60.47fg 1.30.12efg
0.1 3.50.20abc 1.80.20b 2.20.22ef 4.00.54efg 1.40.15defg
0.5 3.30.24abc 1.90.25b 2.70.25bcdef 4.40.57cdefg 1.30.11efg
1.0 3.30.25abc 1.90.19b 2.60.24bcdef 4.90.59cdefg 1.40.01defg
2.5 3.30.20abc 1.90.22b 2.10.25cdef 4.30.72abcdefg 1.70.12bcdef
5.0

2.90.23c 2.30.31b 2.60.27bcdef

4.00.57efg 1.80.14bcdef

121

23 2 (2550)

1 ()

(mg/l) (Mean SE)


(Mean SE) (Mean SE)**
(Mean

TDZ

SE) (Mean SE)**

0.1 3.20.21bc 2.00.21b 2.50.22bcdef 3.70.38fg

0.5 3.50.27abc 1.70.18b 2.60.26bcdef 3.80.39fg
1.0 3.30.20abc 1.90.18b 2.40.25cdef 4.10.45defg
2.5 3.30.30abc 2.20.43b 2.40.27cdef 4.10.49defg
5.0 3.20.26abc 1.50.23b 2.20.18ef 3.80.33efg
0.1

2iP

4.10.19a 2.10.46b

3.30.31ab

0.5 3.80.25ab 1.70.26b 3.00.27abcde

1.0 3.70.25abc 2.10.27b 2.70.21bcdef
2.5 3.50.28abc 2.10.20b 2.90.23abcdef
5.0 3.30.24abc 2.10.24b 2.70.19bcdef

0.1 3.40.28abc 2.00.25b

0.5 4.10.35a 1.80.21b
Zeatin 1.0 3.80.26ab 2.30.27b

3.20.24abc

6.80.78a

1.60.15cdef
1.60.01cdef
1.70.19bcdef
1.50.14def
1.40.14defg
2.20.13bc

4.70.59bcdefg 2.00.18bcde
6.10.57abcd 2.00.12bcd
5.40.66abcdef 1.90.19bcde
5.40.70abcdef 1.80.19bcdef
6.40.73ab

2.20.15b

3.20.20abc

5.90.49abcde 1.90.11bcde

3.20.29abc

6.31.38abc 2.00.12bcd

2.5 3.70.18abc 1.70.18b 3.50.29a 6.31.38abcdef 2.80.12a

5.0 3.40.36abc 3.60.38a 2.60.20bcdef 5.50.93abcdef 0.90.11g

: *
95% Duncans New
Multiple Range Test

**

122

SWU Sci. J. Vol. 23 No. 2 (2007)

2 VW (2549) 10

.

. Kinetin 5.0

. BA 5.0

. TDZ 5.0

Dendrobium Green Lantern
2.5

2.5

2.5
2.5

Dendrobium Green Lantern 2.5
2.5
88.6 89.6
8 ( 3 4)
2.5
2.5

3 Dendrobium Green Lantern

8

 23 2 (2550)

123

4 Dendrobium Green Lantern

2.5 () 2.5
() 8

Dendrobium Green Lantern
VW 2iP 0.1 Zeatin 0.5
(4.1 ) Zeatin 2.5
3.5
2.8 Zeatin 5.0
3.6
VW 2iP 0.1 6.8
Dendrobium Green Lantern 2.5
2.5
8 88.6 89.6

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SWU Sci. J. Vol. 23 No. 2 (2007)

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24 2550
5 2550