Code: 9A23304

1
MICROBIOLOGY
(Bio Technology)

B.TECH II Year I Semester (R09) Regular & Supplementary Examinations November 2012

Time: 3 hours
Answer any FIVE questions All questions carry equal marks ***** 1.

Max. Marks: 70

Explain the identification and cultivation of viruses. Add a note on assay on bacterial viruses.

2. (a) Write briefly about viruses of bacteria. (b) Describe selective media with example. (c) Explain aerobic metabolism. 3. Describe briefly various factors influencing bacterial growth.

4. (a) Explain flagellar staining technique. (b) Write about phylogenetic system of classification. (c) What are the contributions of Edward Jenner to microbiology? 5. (a) Write general characters of Fungi. (b) What are the properties of viruses? (c) What are the applications of virology in biotech industry? 6. (a) Describe blight disease of potato. (b) Write briefly the applications and limitations of various methods of preservation of microorganisms. 7. Explain the major characteristics used in classification of microorganisms (Taxonomy).

8. (a) Write about germ theory of diseases. (b) Describe Whttaker five kingdom classification. (c) Write about IMVIC test.

*****

Code: 9A23304

2
MICROBIOLOGY
(Bio Technology)

B.TECH II Year I Semester (R09) Regular & Supplementary Examinations November 2012

Time: 3 hours
Answer any FIVE questions All questions carry equal marks ***** 1.

Max. Marks: 70

Define nutrition. Explain the sources and physiological functions of macro and micro nutrients.

2. (a) Write briefly about viruses of arthropods. (b) Write about hyophilisation. (c) Explain briefly anaerobic metabolism. 3. Describe identification and in-vitro culture of viruses. Add a note on assay of animal viruses.

4. (a) Explain principle gram staining technique. (b) Describe Carl Woose et-al system of classification. (c) Write about theory of spontaneous generation. 5. (a) Explain structure and life cycle of TMV. (b) Describe briefly the virulence of tuberculosis. (c) What are the contributions of Robert Coach to the field of microbiology? 6. (a) Describe the process of replication of plant viruses. (b) Explain briefly catalase production test. 7. Write about general characters of Eubacteria.

8. (a) Write the general characters of protozoa. (b) Explain effect of temperature on growth of microorganisms. (c) Describe the technique used in cultivation of anaerobes. *****

Code: 9A23304

3
MICROBIOLOGY
(Bio Technology)

B.TECH II Year I Semester (R09) Regular & Supplementary Examinations November 2012

Time: 3 hours
Answer any FIVE questions All questions carry equal marks ***** 1. Give an account of different methods of pure culture techniques.

Max. Marks: 70

2. (a) Describe briefly the replication of bacterial viruses. (b) Explain the structure of T4 phase. (c) Write about sugar fermentation test. 3. Explain the virulence and virulence factor of influenza.

4. (a) Explain the influence of PH on growth of bacteria. (b) Write about synthetic media used in cultivation of microbes. (c) Describe plylogenetic system of classification. 5. (a) Explain Whittakers five kingdom classification. (b) Write the general characters of bacteria. (c) Describe the viruses of bacteria. 6. (a) Explain the application of virology in biotech industry. (b) Describe applications and limitations of preservation methods of microbes. 7. Explain the identification and culture of viruses. Add a note on assay of bacterial viruses.

8. (a) Write the general characters of Archae bacteria. (b) What are the contributions of Leis pasture to microbiology? (c) Explain different nutritional groups of microorganisms. *****

Code: 9A23304

4
MICROBIOLOGY
(Bio Technology)

B.TECH II Year I Semester (R09) Regular & Supplementary Examinations November 2012

Time: 3 hours
Answer any FIVE questions All questions carry equal marks ***** 1.

Max. Marks: 70

Define culture media. Explain in detail different culture media used for cultivation of microorganisms.

2. (a) Describe assay of animal viruses. (b) Explain briefly the replication of bacterial viruses. (c) Write about viruses of arthropods. 3. Describe different methods preservation of microorganisms. Add a note their applications and limitations.

4. (a) Explain IMVIC test. (b) Write the general characters of Eubacteria. (c) What are the contribution of Leuven hoek to microbiology. 5. (a) Explain the structure of TMV. (b) Write about germ theory of diseases. (c) Describe various growth factors and their functions in metabolism. 6. (a) Write briefly about classification of viruses. (b) Describe virulence factors of blight disease of potato. 7. Explain various types of staining technique employed to study the microbial colony characters.

8. (a) Describe the applications of virology in biotech industry. (b) Explain the Carl Woese system of classification of microbes. (c) Write briefly about pure culture methods. *****