1.

Cc phng php in vitro trong kim tra nh gi hot tnh khng khun:

Phng php Nguyn tc s dng Cch thc hin

o c c ch tng trng vi khun Trong mi trng lng (1).
(Turbidometric assay ch th bng cc sn phm Cho dch ly tm mi trng
method) trao i cht ca Vi khun. (2) nui cy LAB vo mi
trng pht trin ca vi
khun ch th. Sau 21h o OD
bc sng 600nm, so snh
phn tram vi ng i chng
khng s dng dihcj ly tm
trn.

Khuch tn qua vng giy loc c ch tng trng vi khun Trong mi trng agar (3).
( Disc diffusion assay) ch th bng cc sn phm Thm giy vo dch ly tm
trao i cht ca Vi khun. mi trng (2) nui cy LAB.
t giy thm ln b mt mi
trng agar tri vi khun
ch th trc . qua m,
kim tra vng khng khun.

Khuch tn qua ging thch c ch tng trng vi khun Trong mi trng agar (3).
(Well diffusion assay) ch th bng cc sn phm Tri vi khun ch th, c l
trao i cht ca Vi khun. ng knh 8mm. Cho dch ly
tm mi trng (2) nui cy
LAB. qua m, kim tra
vng khng khun.
 u tranh trc tip ca cht Ging nh trn. Tuy nhin s
vi vi khun ch th dng trc tip dch nui cy
LAB. qua m, kim tra
vng khng khun.
Spot on lawn u tranh trc tip ca cht Trong mi trng agar (4).
vi vi khun ch th Nh dch cha t bo LAB,
c nh bng mt git mi
trng (5). hn hp vi
khun ch th vi 7ml mi
trng (5) ln, ng, c
hai mt kp ca hai mi
trng (4) v (5). qua m,
kim tra cng khng khun.

Khuch tn trn b mt thch u tranh trc tip ca cht Trn mi trng agar (3).
(Agar spot test) vi vi khun ch th Tri vi khun ch th, nh git
mi trng (2) nui cy LAB.
qua m, kim tra vng
khng khun.

Gii thch thm:

Mi trng (1) BHI Broth
Mi trng (2) MRS Broth
Mi trng (3) BHI Agar 2%
Mi trng (4) MRS Agar 2%
Mi trng (5) BHI Agar 0,7%
Kim tra vng khng khun l o vng trong sut khng c s pht trin ca vi khun ch th.
Minh ha cc phng php kim tra hot tnh khng khun