Section A

Section A has 12 multiple choice questions. Each question has five options for an answer.
There is only one correct answer. Circle the most appropriate answer. In this section no
marks will be deducted for wrong answers (20 marks).

1. Deoxyadenosine
a) has uracil as its base
b) is a nucleoside
c) is found in RNA
d) is found in DNA
e) is phosphorylated

2. RNA
a) is a polynucleotide
b) is single stranded
c) is always double stranded
d) does not have thymine base
e) does not have ribose

3. Purine bases include

a) Cytosine
b) guanine
c) adenine
d) Thymine
e) Uracil

4. Nucleosides differ from nucleotides because nucleosides have

a) no bases
b) no phosphate
c) have multiple phosphates
d) have a glucose instead of a ribose
e) have only purine bases

5. Which one below is a pyrimidine nucleoside?

a) Adenosine
b) Deoxycytidine
c) Guanosine
d) Deoxycytidylate
e) Uridylate

6. How many phosphoanhydride bonds are in ATP?

a) 2
b) 1

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 c) 5
d) 3
e) 6

7. 7-methylguanosine triphosphate capping is found in

a) mRNA
b) rRNA
c) tRNA
d) DNA
e) Glycogen

8. Deoxyadenosine
a) has uracil as its base
b) is a nucleoside
c) is found in RNA
d) is found in DNA
e) is phosphorylated

9. RNA
a) is a polynucleotide
b) is single stranded
c) is always double stranded
d) does not have thymine base
e) does not have ribose

10. Purine bases include

a) Cytosine
b) guanine
c) adenine
d) Thymine
e) Uracil
11. Nucleotides
a) serve as energy currency in metabolic transactions
b) are fatty soluble vitamins
c) form essential links of cells in response to hormones and other
extracellular stimuli
d) are structural arrays of coenzymes and metabolic intermediates
e) are constituents of RNA and DNA

12. Nucleotides unlikely to be found in RNA include

a) Adenylate
b) thymidylate
c) Guanylate
d) deoxycytidylate
e) Uridylate

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Section B has 10 parameter in one column of the table. Match each parameter in the other
column of the table. Half a mark will be deducted for each wrong answer (20 marks).

S/N Parameter Parameter

1 Minor base Palindrome
2 2-Amino-6-oxypurine Nucleic acid
3 Nucleoside B-DNA
4 Nucleases Digest nucleic acid

5 Nitrogenous base alkylation

6 DNA Adenine
7 Nucleotide Ribose
8 Sugar cAMP
9 DNA sequence Guanine
10 Diameter 20Ǻ Guanosine

Section C has five essay type questions.

You must answer question 1 which is compulsory (20 marks)
From the remaining four, answer any two questions (15 marks each)

1.Explain the functions of nucleotides? 20marks

2. Write short notes on denaturation of DNA 15marks

3. a. Calculate the final melting temperature for a dsDNA molecule whose

Initial Tm= 78oC, if it is placed in a 90% formamide solution 10marks
b. A sample of double stranded DNA was found to have thymidylate as
23% of the nucleotide residues. What should be the %GC of this DNA?
5marks

4. Write short notes on denaturation of DNA. 15marks

5. How does DNA differ from RNA? 15marks