Name: _________________________________ Grade & section: ________________

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Grade 10 Activity Sheet on Protein Synthesis

Objective
Apply the concept of protein synthesis through the amino acids coded for the mRNA codon using the genetic code.

Procedure
1. Study the given scenario (see attached activity) and study the profile of the suspects and additional information.
2. Using your knowledge about DNA and RNA base-pairing, decode the DNA sample in order to identify the sequence of
amino acids. Use the amino acid identifier chart as your reference.
3. Compare the sequence of amino acids (proteins) to the profile of the suspects and additional information given in
order to determine the corresponding traits.
4. Name the culprit using the data or information you got.
5. Using the pencil and crayons make a cartographic sketch of the suspect.

Decoding DNA Segment

An intruder razed the science laboratory of a Junior High School. The school is in chaos. The school administrators and teachers
demanded to know the culprit. They have to identify the culprit and do it fast. They had three suspects namely Rudy Tee, Von Go and
Rambo Tan. As a forensic expert, your mission is to identify the intruder using the profile of the suspects and other information taken
from the laboratory:

THE PROFILE OF THE SUSPECTS

Rudy Tee
 Flunked physics class three times Rambo Tan
 with long nose hair,  Suspended for a week by the Disciplinary Committee for
 oval eyes repeated tardiness.
 full lips  short nose hair
 attached ear lobe  oval eyes
 straight hair  harelip
 attached earlobe
Von Go  wavy
 His investigative project is about the potential applications of nuclear fusion in generating energy.
 short nose hair
 chunky eyes
 full lips
 free earlobe
 curly hair

Additional Information: Below are the specific amino acid sequence for specific trait.

1. Nose hair
 Short: phe-lys
 Long: leu-lys 4. Ear attachment
 Free earlobe: thre- tyr-ser
2. Shape of Eyes  Attached: leu-arg-gly
 oval: val-ala-ala
 chunky: val-ala-val 5. Type of hair
 Straight: lys-glu
3. Lips  Wavy: phe-ala
 Full lips: phe- glu-ala
 Curly: glu-phe
 Harelip: phe-lys-ala

THE DNA CODE OF THE SUSPECT

A fresh DNA sample was obtained from the laboratory; believe to have been left by the suspect. Using the DNA sequences, the
following nucleotide sequence was obtained:
SUSPECT: TAC-AAA-TTT-ATC-TAC-AAA-CTT-CGT-ATC-TAC-CAT-CGT-CAT-ATT-TAC-TGG-ATA-TCG-ATC-TAC-
CTT-AAA-ATC
Complementary base pairs:
DNA is represented by nitrogen bases that are read in groups of threes. To decode, use the DNA as a blueprint to produce
mRNA. Then use the mRNA as blueprint to produce the linear sequence of tRNA. A specific nitrogen base in the DNA pairs up with a
specific nitrogen base in the mRNA, and a specific nitrogen base in the mRNA pairs up with a specific nitrogen base in the tRNA.

Breaking the Code: Complete the data table below.

DNA triplet code mRNA tRNA Amino acid

Characteristics and traits of the suspect based on the DNA code.

1. Nose hair: ___________

2. Eyes: ___________
3. Lips: ___________
4. Ear attachment: ___________
5. Type of hair: ___________

___________________________________________
Name and cartographic sketch of the suspect