Chapter III

METHODOLOGY

This chapter presents the research design, methods and materials, collection of data, statistical

treatment and paradigm.

Research design

The experimental research design will use in this study to know the effectiveness of guava extract

as anti-microbial component of mouthwash. Furthermore, this research will use the two group design in

which to be tested, the controlled group and experimental group. The data gathered were categorized and

sorted into patterns as the primary basis for organizing and reporting the study findings in order to assess

the effectiveness of the guava extract.

Methods and materials

A. Preparation of the materials

In preparation of the guava mouthwash solution, the researcher will prepare the needed

materials, guava leaves, tumbler or small container and grinder or blender.

B. Guava Mouthwash making

Materials:

Tumbler/container

Grinder or blender

Ingredients:

Dried guava leaves

Salt

Baking soda

Alcohol

Water
 C. Procedure

1. Pick guava leaves, clean it with water and let it dry under the sun.

2. Harvest the dried guava leaves.

3. Pulverize the guava leaves with the use of blender.

4. Get 1 tsp baking soda, 1 tsp salt and 1 tsp alcohol, mix them together.

5. Add the pulverize guava leaves, stir until mix into the mixture.

6. Place the it in a stoppered tumbler or container.

7. Let it settle up to 7 days.

8. After 7 days get the mouthwash solution

Collection of Data
In collecting the data, the researcher will observe the experimental group and controlled group

using the mouthwash solution. The researcher will carefully record the progress of each group in using

the mouthwash solution.

Statistical Treatment
The researcher will use t-test where in it is use to compare two means, the meansof two

independent samples or two independent groups and the means of correlated samples before and after the

treatment. It is also used to compare the means of two unrelated groups of samples.The t test statistic

value to test whether the means are different can be calculated as follows:
Where:

x1 = mean of first set of values

x2 = mean of second set of values

ss1 = standard deviation of the first set of values

ss2 = standard deviation of second set of values

n1 = total number of values in first set

n2 = total number of values in second set

Research Paradigm

Independent Variable dependent Variable