LABORATORY REPORT IN

MICROBIOLOGY AND PARASITOLOGY

Madam Jaraba, Khyla Exercise 2

BS Psychology 1A March 15, 2023
Associate Professor Carina C. Batol March 16, 2023

MAKING A WET MOUNT

I. Objectives
1. To make a wet mount of onion, tomato, cheek cell, and sperm cell.
2. To differentiate the cell of onion, tomato, and cheek cell in Low Power Objective and High-Power
Objective.
3. To draw and color the onion, tomato, and cheek cell.
4. To appreciate the variety of cell shape, size in tomato, onion, and cheek cell.

II. Materials Needed

1. Fresh Mount Slide
2. Cover Slip/Cover Glass
3. Compound Microscope
4. Beaker
5. Dropper
6. Water
7. Iodine
8. Tomato
9. Onion
10. Cheek Cell
11. Sperm Cell

III. Methodology

1. Prepare your specimen: Collect a thin slice of your sample and place it on a clean, dry slide.
2. Add a drop of liquid: Add a drop of water, saline solution, or any other liquid that will provide a suitable
medium for the specimen to remain visible.
3. Place a coverslip: Palace the cover slip at a 45-degree angle with one edge touching the water and let go.
The coverslip will fall over the sample and be held in place by surface tension.
4. Seal the edges: Use a piece of tissue paper or a lens wipe to remove excess liquid from the edges of the
coverslip.
5. Label and observe: Label the slide, and observe the specimen under the microscope. Adjust the focus and
illumination as necessary to obtain a clear image.
6. Disposal: once you are done with your observation, dispose of the slide and coverslip in a container
designated for the biological waste.

IV. Data and Results

PLANT CELL DESCRIPTION FUNCTION

1. Cell Membrane Made up of a matrix of pectin Provides tensile strength and protection
polysaccharides that is highly cross- against mechanical and osmotic stress
linked and contains a network of
cellulose microfibrils and cross-linking
glycans.
2. Lysosome A sac-like compartment inside a cell that The digestive system of the cell
has enzymes that can break down
cellular components that need to be
 destroyed
3. Cytoplasm Helps keep organelles suspended, the
internal structure of the cell supported,
and the plant cell's shape is maintained.
4. Nucleus A membrane bound organelle that
contains the DNA of the cell
5. Nuclear A double layer that encloses the cell's
Membrane nucleus, where the chromosomes reside
6. Nucleolus Less organelle within the nucleus that
manufactures ribosomes, the cell's
protein-producing structures
7. Vacuole Providing structural support, as well as
serving functions such as storage, waste
disposal, protection, and growth
8. Golgi Body The site at which the complex
polysaccharides of the cell wall are
synthesized.
9. Mitochondria Oblong shaped organelles found in the
cytoplasm of all eukaryotic cells
10. Rough ER A cellular organelle composed of many
folds of tissues and channels
11. Smooth ER Tube-like structure located near the cell
periphery
12. Ribosome Small circular organelles found in the
cell
13. Cell Wall Composed of a network of cellulose
microfibrils and cross-linking glycans
embedded in a highly cross-linked
matrix of pectin polysaccharides
14. Chloroplast Convert light energy into relatively
stable chemical energy via the
photosynthetic process
ANIMAL CELL DESCRIPTION
15. Cell Membrane Separates the interior of the cell from the
outside environment
16. Lysosome A membrane-bound cell organelle that
contains digestive enzymes.
17. Cytoplasm A gel-like material that fills the area
between the plasma membrane and the
nuclear membrane
18. Nucleus It is both the largest and stiffest
organelle and is easily identifiable by
light microscopy
19. Nuclear A double layer that encloses the cell's
Membrane nucleus, where the chromosomes reside
20. Nucleolus The site of ribosome biogenesis
21. Golgi Body A cell organelle that helps process and
package proteins and lipid molecules,
Holding the components of the cell and
protects them from damage.
Store DNA or hereditary information
required for cell division, metabolism
and growth
Separate the chromosomes from the
cell's cytoplasm and other contents
Facilitating ribosome biogenesis
Maintaining cell acidity and turgor
pressure, regulating the storage and
transport of substances, controlling the
transport and localization of key
proteins through the endocytic and
lysosomal-vacuolar transport pathways,
and responding to biotic and abiotic
stresses.
Transporting, altering, and packing
proteins and lipids to specific locations
are the main duties of this cell organelle
Produce energy through cellular
respiration
To produce proteins for the rest of the
cell to function
Make cellular products like hormones
and lipids
A micro-machine for making proteins
Provides tensile strength and protection
against mechanical and osmotic stress
Accumulate the high levels of colourful
pigments in plant tissues or organs
FUNCTION
To be a barrier keeping the constituents
of the cell in and unwanted substances
out
The digestive system of the cell
Holding the components of the cell and
protects them from damage
Store the cell's DNA, maintain its
integrity, and facilitate its transcription
and replication.
Separate the chromosomes from the
cell's cytoplasm and other contents
Produce and assemble the cell's
ribosomes.
A factory in which proteins received
from the ER are further processed and
 especially proteins destined to be
exported from the cell
22. Mitochondria Membrane-bound cell organelles
(mitochondrion, singular) that generate
most of the chemical energy needed to
power the cell's biochemical reactions
23. Rough ER Involved in some protein production,
protein folding, quality control and
despatch
24. Smooth ER Devoted almost exclusively to the
manufacture of lipids and in some cases
to the metabolism of them and
associated products
25. Ribosome An intercellular structure made of both
RNA and protein, and it is the site of
protein synthesis in the cell
sorted for transport to their eventual
destinations: lysosomes, the plasma
membrane, or secretion
Generate most of the chemical energy
needed to power the cell's biochemical
reactions
To produce proteins that will become
part of the endomembrane system, the
plasma membrane or to be secreted.
To make cellular products like
hormones and lipids
The site of protein synthesis in the cell

V. Drawing

VI. Answers to questions

1. What are the two general types of cells?
- Prokaryotic and eukaryotic cells are the two general types of cells.

2. Differentiate the two.

- Prokaryotic cells are always unicellular, but eukaryotic cells are frequently multicellular creatures.
Eukaryotic cells are also 100 to 10,000 times larger and far more complicated than prokaryotic cells.
Prokaryotic DNA is stored in the cytoplasm, whereas eukaryotic DNA is stored in the nucleus.

VII. Interpretation of data and result

The table depicts plant and animal cell differentiation. It also describes the parts, descriptions, and
functions of each cell.

VIII. Conclusion

As a result, I come to the conclusion that wet mounts are employed to see microorganisms. The
specimen is supported by the water's refractive index, which increases image quality even further. Water fills
the gap between the coverslip and the slide, securing the sample. Light can readily pass through the coverslip,
sample, and slide as a result. The wet mount method should be used to determine whether or not an organism
is motile.

IX. References

https://elementalscience.com/blogs/science-activities/how-to-make-a-microscope-slide