Polymer nhy nhit cho qu trnh chuyn ha thuc

Kh nng phi tri qua nhng thay i nhanh chng p ng vi tn hiu mi trng
nhy cm lm cho cc vt liu p ng kch thch tr thnh ng vin cho s xam6
nhp ti thiu cho mc tiu chuyn ha thuc cho con ngi. Bo co ny m t ngn
gn v tm quan trng v cch tng hp vt liu nhy nhit v lin quan ti ng dng
trong chuyn ha thuc. Bi bo co ny xem xt u im v nhc im ca vt liu t
nhin so vi vt liu tng hp v c im chnh ca cc vt liu xp khc nhau c s
dng nh vt liu nhy nhit trong chuyn ha thuc. Tc gi cung cp bi bnh lun
v trng thi hin ti ca lnh vc ny v cung cp ci nhn su sc ca h vo nhng k
vng trong tng lai chuyn ha thuc do nhy nhit. Nhn mnh tm quan trng
Vt liu p ng kch thch c c nhng thnh tu ng ch cho tim nng v
mc tiu, c th iu chnh v c nhn ha tr liu, ci m p ng trc tip n mi
trng sinh l ca c th. Vi nhng tin b gn y trong cng ngh polyme ha dc,
n ang tr nn ngy cng c th bin i phng thch thuc..
Polyme p ng kch thch cng gi l polyme thng minh, p ng mi trng v
polyme cm ng, nh mt loi vt liu vt qua ng k v thay i c tnh ha l
p ng c s thay i nh trong iu kin mi trng. y l mt loi polymer cm
ng, c kh nng p ng li nhiu kch thch nh nh sng, pH, nhit , sng siu m,
lnh vc in, cc enzym, khng th hoc s hin din ca cc phn t c bit nh
glucozse. Hn na, cch phn ng li khc nhau ty tng vo h thng. Polymer c th
tri qua s thay i v cn bng gia a nc v k nc, tan, s hydrat ha, cu to,
hnh dng, s phn hy, micell ha trong s p ng li s hin din hay mt i ca mt
tc nhn bn ngoi. Do n c th c s dng to ra khung chuyn ha thuc p
ng li kch thch, c th iu khin c, cch thc p ng c xc nh trc bit
c mt lng ln ca s iu khin v c th iu chnh c liu lng thuc. Kh
nng to ra mc tiu trn yu cu phng thch thuc c tng quan vi mt lng ng
dng lm sng khc nhau bao gm liu php thay th hormon, ha tr iu tr, ri lon
nhp tim, bnh tiu ng, kim sot sinh . Trong s cc vt liu khc nhau v p ng
kch thch, polymer cm ng nhit c nghin cu nhiu nht v c tm quan trng c
bit trong ng dng phn phi thuc. Polymer cm ng nhit v ng dng trong vic iu
khin s phng thuc
Polyme cm ng nhit c s dng mt cch khn ngoan khi thay i nhit dn n
s thay i v m trong tnh cht ca vt liu. Polyme m c nhit ha tan ti hn di

(LCST) c s chuyn ha sol-gel c trng khi gia nhit trn LCST. Ngc li polyme
tan c khi gia nhit th c gi l c mt nhit ha tan ti hn trn (UCST). C hai
loi ny c th c dng trong mc ch chuyn ha thuc. Copolyme c LCST c th
c trn (pha ln) mt cch n gin vi thuc to h huyn ph lng nhit phng
v c chuyn ha thng qua k thut tim xm ln ti thiu trc tip n cc m kh
tip cn trong c th. S gia nhit n nhit c th xy ra qu trnh chuyn ha sol-gel,
thuc c by trong lng polyme v duy tr phng thuc lin tc ti ni mong mun.
Polyme chuyn ha thuc c nhit ha tan ti hn trn c th dng nhit lm gin
n hoc lm cu trc mt n nh nhanh chng phng thuc ti tm mong mun. Gia
nhit nh v (m khi u) hoc p dng kch thch t bn ngoi ( sng siu m, tia hng
ngoi..) c th c s dng to ra s mt n nh cc b ca mt copolyme chuyn
ha thuc c nhit ha tan ti hn trn phng thuc vo mc tiu.
Polyme chuyn ha thuc cm ng nhit c rt nhiu li ch nh phi loi b tnh cn
thit ca vic gii phu xm ln v c kh nng vt qua ro cn sinh l. cho php
chuyn ha n cc v tr kh tip cn trong c th. Hn na, vic cha thuc trong cu
trc khung ca polyme c th bo v tc nhn tr bnh khi enzyme hoc s suy thoi ca
mi trng.
Tng hp vt liu cm ng nhit.
Poly (N-isopropylacrylamide)
Polymer gel nc cho thy s thay i nghch o th tch t c nhiu li ch ng
k t khi k t khi Tanaka quan st cc xu hng gel polyacrylamide tri qua s tch
pha p ng vi nhit , thnh phn cht lng. Poly (N-isopropylacrylamide)
(PNIPAAm) l mt trong nhng nghin cu rng ri nht v polyme cm ng nhit, n
chuyn ha sol-gel nhanh chng khi gia nhit trn nhit ha tan ti hn di ca n,
khong 32oC. cho php tim vo nhit phng v s hnh thnh gin gio nhit
c th. Mt trongnhng hn ch ln ca PNIPAAm l n khng th phn hy.Vt liu
chuyn ha thuc phn hy c c th loi b cc gin gio sau khi phng thuc m
khng i hi phu thut ct b. Do , im nhn mnh ny tng trong nhng nm gn
y v vic gii thiu kh nng phn hy trong copolyme da trn NIPAAm. Do cn gi
tnh cht chuyn ha pha di tc dng ca nhit ca NIPAAm, trong khi y mnh s
phn hy cui cng, gii phng ra khi c th sau khi ht tc dng iu tr. Nm 1999,
Neradovic tng hp polyme da trn NIPAAm vi 2-hydroxyethylmethacrylatemonolactate. (HEMA-monolactate). Khi thy phn nhm ester lactate khng bn lm
tng tnh a nc ca copolyme, do lm tng nhit ha tan ti hn di. Nu LCST
tng trn nhit c th. Copolymer da trn NIPAAm nhit thun nghch tr li trng

thi hydrat ha, cho php hp thu vo h tun hon v gii phng khi c th min l
trng lng phn t thp hn gii hn lc thn. Yoshida thit k copolyme da trn
NIPAAm crosslink vi poly(amino acid) c th phn hy c cho thy c ch khong
trng tng t. Guan tng hp mt chui protein hot tnh, copolyme da trn
NIPAAm, c bn ko cao, thy phn ha tan c, sn phm phn hy khng c
hi trong sut qu trnh ng trng hp vi HEMA-polylactide (HEMA-PLA), Nacryloxysuccinimide v acrylic acid. Ma ci thin tnh cht c l thng qua vic kt
hp ca methacrylate-polylactide (MA_PLA) v HEMA. Cui pht trin mt lot cc
copolyme da tren NIPAAm phn hy chm thng qua qu trnh ng trng hp vi
dimethyl butyrolactone (DBA). Cc copolyme ny phi tri qua qu trnh thy phn m
vng lactone DBA, c kh nng lm tng LCST trn nhit c th m khng to ra bt
c sn phm phn hy no. Nhm Sheardown chun b hot tnh sinh hc ca
copolyme NIPAAm/DBA thng qua qu trnh ng trng hp vi N-acryloxysuccinimide
cho on sau cha mt v cho mc ch phn phi thuc. Cc loi homopolymer v
copolyme cm ng nhit ca PNIPAAm, c rt nhiu v d cho polyacrylamide p ng
nhit ca nhm th N c th thch hp trong phn phi thuc nh poly (N,N
diethylacrylamide) v poly(n-vinylcaprolactam). Tng t nh PNIPAAm, polyme mch
thng nh poly(N,N-diethylacrylamide v poly(N vinylcaprolactam) c gi tr LCST l
32oC v 31oC.
Copolyme cm ng nhit da trn polyethylenoxide
Triblock copolyme c cu trc A-B-A c hnh dng ca polyethyleneoxidepolypropyleneoxide-polyethyleneoxide (PEO-PPO-PEO), gi tt l Pluronic hoc
Poloxamers l thnh vin ca vt liu tng hp keo ha nhit c nghin cu rng ri
cho tim nng ca n s dng nh cht mang thuc. Thng qua nng , thnh phn v
trng lng phn t, cc copolyme ny c th c iu khin chu c s chuyn
ha gel nhit v pH ca c th. S lin kt gia ethylene oxide a nc v popylene
oxide k nc to thnh mt copolyme c c phn a nc v k nc c th t lin kt
thnh cc micell trong iu kin dung dch nc khi trn nng micell ti hn (CMC).
CMC l ph thuc nhit cao, nh di nhit micell ti hn, c ethylene oxide v
propylene oxide khi l u tan tng i trong nc. Khi tng nhit h ln, mch
PPO tr nn t tan dn n hnh thnh micell. Micell Pluronic c trng c ng knh t
10-100nm v gm phn k nc, core l PPO v phn hydrate, shell l PEO a nc. Li
PPO c kh nng kt hp ln n 30% trng lng thuc khng tan trong nc trong khi
vnh PEO cha cc micell trong trng thi phn tn v ci thin s n nh ca thuc
bng cch cch ly vt lu tr t s tng tc khng mong mun vi cc t bo v protein.
Tuy nhin cc hydrogel c bn c l km, bn v tnh thm cao b hn ch. Nh

hn ch ny m duy tr c qu trnh phng thuc hiu qu. Cohn s dng mt s

chin lc nng cao tnh cht c l ca copolyme PEO/PPO. Nh vy, hng ny bao
gm s a vo lin kt nhm cui mch bng phng php insitu, nh ni i C-C,
nhm methacrylate v triethoxysilane v lin kt cng ha tr PEO v PPO s dng
carbonyl chloride v cu ni diacyl chloride. Trong nhng nm gn y, c nhiu kho
st trong tng hp copolyme ca PEG vi polyester c th phn hy nh PLA, PLGA v
PCL to ra copolyme cm ng nhit ci thin cng gim trong c th. PEG cha
copolyme thu ht s quan tm ng k bao gm PLGA,
Polyphosphazenes
Polyphosphazenes l mt loi polyme p ng nhit l s kt hp gia hp phn v c v
hu c. Cc loi polyme ny c cha mt sn hp cht v c bao gm s thay th
nguyn t hydro v phospho lin kt vi nhau thng qua cc lin kt n v cc lin kt
i. Gn vi mi nhm phospho l hai nhm hu c cho thy linh ng cao cho vic
bin tnh d dng hoc thao tc(gia cng) v tnh cht v nhm chc, ci c s dng
truyn t cho tnh cht cm ng nhit. Nhiu phng php tip cn c tm hiu
pht trin copolyme c th thy phn c, iu khin c ng hc ca qu trnh phn
hy, c kh nng phn hy qua cc giai on khc nhau c th ko di t vi gi n vi
nm. Polyphosphazenes c chng minh c kh nng tng thch tt vi nhiu loi
t bo trong c th. Hn na, cc sn phm ph ca qu trnh phn hy, c th l amonia,
phosphate v ancohol th chu c tt v copolyme c thit k c qu trnh gel ha
xy ra nhanh chng ngay ti ch vi tc phng thch c th iu khin c, lm cho
polyphosphazene tr thnh ng c vin cho chuyn ha thuc
Vt liu cm ng nhit t nhin
Elastin like polypeptides
Elastin like polypeptides l polyme c tng hp da trn elastin vi cu trc c s lp
li ca amino acid pentapeptides Val-Pro-Gly-XAA-Gly, XAA c th l bt k amino
acid t nhin no ngoi tr cc acid bo [39,40]. Di nhit chuyn pha (Tt). ELPs
vn cn tn ti nh mt dung dch ng nht trong nc. Khi gia nhit ln trn nhit
chuyn pha ca n, dung dch tr nn c do ELP t li thnh git. Kch thc v s
phn phi cc git ELP c th thay i thng qua nng v nhit . Trong nhit
chuyn ha c th iu chnh bng cch thay i nng , trng lng phn t, hm
lng mui v thnh phn ELP (v d thng qua vic bin tnh d lng Xaa, s dng
cc amino acid khc nhau bng cch nhm chc ha cc protein hoc cc polymer. ELP
c dng trong phn phi thuc khi tnh cht ca n c th iu khin c, dung np

tt trong c th v phn hy thnh cc amino acid n gin. Hn na, trng lng phn
t v thnh phn ca ELP c th c kim sot chnh xc thng qua cc phng php
k thut di truyn to thnh polyme c a phn tn hp, iu ny lm tng kh nng
iu khin qu trnh phng thuc hiu qu. Hn na, ELP c th c th hin s
lng cao t Escherichia coli v chuj th d dng tinh ch nh l kt qu ca qu trnh
gel ha bi nhit. Nhm Chilkoti nghin cu ELP cho m khi u. Bng cch kt hp
thuc vi ELP nhit chuyn pha cao hn nhit c th. Cc ELP nh, tan c th
rt thun li tng tnh thm trn mch khi u tch t trong cc m khi u sau khi
chuyn ha. Cch khc, ELP v thuc kt hp vi nhau c thit k dit khi u bi
nhit, cc peptide c thit k c Tt khng t 37-42oC v khi p cc kch thch bn
ngoi lm tng nhit cc b dn n dsu75 kt ni gia ELP v thuc kt hp li v bm
cht vo thnh mch. Khi nhit thn nhit tng nh

ng dng ca polymer pluronic F127 nhy cm bi nhit trong iu tr cc tn thng bng

Use of polymer pluronic F127 sensitive to temperature in treatment of burns
Trn Hu Dng

S: 463 - Thng 11/2014 - Trang 2-9, 39

Pluronic, cn c cc tn gi khc l lutrol, monolan, poloxamer, l cc co-polymer khng ion ha ca

polyoxyethylen -polyoxypropylen - polyoxyethylen (PEO PPO PEO), trong cc phn on
polyoxyethylen l a nc cn cc phn on polyoxypropylene li k nc. Tt c cc pluronic u c
cu to ha hc ging nhau, ch khc nhau s lng tng i ca polyoxyethylen
polyoxypropylen, chnh v th m th cht, trng lng phn t cng nh c tnh hot ng b mt ca
mi loi l rt khc nhau. Trong , hu ht cc pluronic l cc cht rn c kh nng tan trong nc v
chng ha tan trong nc lnh nhiu hn trong nc nng, l kt qu ca vic gia tng s solvat ha
v lin kt hydro nhit thp. iu ny c gii thch bi trong nhit thp s tn ti mt lp
hydrat ha bao quanh cc phn t pluronic trong dung dch nc, lm phn tn chng v kt qu l
cc pluronic c ha tan vo trong nc. Khi nhit nng ln cc chui a nc ca pluronic khng
solvat ha na do s t gy cc lin kt hydro c thit lp gia dung mi v cc chui ny, do
m pluronic khng tan tt trong nc c nhit cao (> 20oC).
Cc pluronic cn c mt c tnh rt c trng l tnh bin i th cht bi nhit . Khi nhit
thp (0 4oC), dung dch pluronic vi mt nng thch hp s tn ti trng thi lng, nhng khi tng
nhit ln nhit phng (20 ~ 25 oC) th li chuyn sang dng gel rn. Tuy nhin, dng gel ny s b
thoi bin v chuyn v dng lng ban u nu h nhit xung.
Nguyn nhn ca s bin i th cht ny l do s hnh thnh cc micell khi tng nng dung
dchpluronic v nhit mi trng xung quanh. Trong mi trng nc nng thp (di 12
15%) chng to thnh cc micell n phn t gm cc phn t pluronic n l, nhng khi nng cao
hn th nhiu phn t tp hp li to thnh cc micell hnh cu bao gm mt li trung tm k nc gia
cha cc chui polyoxypro
S micell ha l s chuyn i trng thi th cht ca polymer d th (bao gm phn thn du v
phn thn nc trong cu trc phn t) t dng lng sang dng rn, xy ra vi cc dung dch pha long
ca polymer trong dung mi c la chn vi nng ln hn nng micell ti hn (cmc) ti mt
nhit nht nh, s to thnh cc micell hnh cu vi cu trc vng chc, v cui cng s chuyn th

cht t trng thi lng sang trng thi rn vi cu to mng tinh th bn vng. Tuy nhin y l qu trnh
chuyn i thun nghch theo nhit mi trng nn thng c gi l s thoi bin bi nhit.
c rt nhiu nghin cu da trn tnh cht c trng ny ca pluronic ch to ra cc dng bo
ch dc phm khc nhau m trong pluronic c dng nh mt t dc chnh. Pluronic c s dng
nh nhng cht nh ha trong cc nh tng du tim tnh mch, cng nh l tc nhn gip ha tan v n
nh duy tr s ng nht ca elixir v siro. Gn y, pluronic c s dng trong cc h thng phn
phi thuc, c bit l pluronic F68 v pluronic F127. Cc loi pluronic ny thng c s dng trong
ng ung nh l cht lm t v bi trn trong iu tr to bn, thng c kt hp vi cc thuc
nhun trng. Ngoi ra cn c s dng trong cc dng thuc nh mt v gel cha cht khng khun iu
tr bng.
Kt qu: Qua tham kho ti liu nhn nh rng vic s dng ti ch ca gel pluronic F127
cho thy c th cha lnh vt thng h mt cch hiu qu v cng c th c s dng nh mt gi
mang phng thch ti ch cc thuc lm lnh cc vt thng khc nhau nh vt thng bng, vt thng
nhim trng ngoi da nhm nng cao hiu qu iu tr. Tuy nhin, hin vn cha c mt loi mng tr
bng trong nc no nghin cu s dng vt liu pluronic ng dng trong iu tr bng hay cc tn
thng ngoi da. V vy, y l mt lnh vc y tim nng cho cho cc nh nghin cu Y Dc Vit
Nam tp trung pht trin, nhm to ra mt loi mng pluronic bn vng c cha khng sinh v cht kch
thch tng sinh t bo, gip cho cng tc iu tr bng din ra kp thi, thun li v gim au n cho
bnh nhn.
Pluronic F127 (PEO101 - PPO56 - PEO101) has exhibited particular interest for long for its easiness of liquid
solid transformation with changes in temperature. At concentrations greater than 20% (w/w), the
transparent pluronic
could immediately
change
into solid gel on
exposure
to high temperatures by formation of spherical micelles with a hydrophobic nuclear inside and
hydrophilic branches outside, creating thecrystalline phase in water. This is the basis for the studies
using pluronic F127 not only as a main adjuvantin formulation of local drug release control systems but
also as polymeric membrane coverings for burn wounds. Pluronic solid membrane prepared at 37C could
served as an artificial barrier to help limit
thewound infection, maintaining the proper
humidity and
prevent dehydration and loss of electrolytes on the wound surface. Besides, this polymeric membrane also
sustained antibiotic release and promote cellproliferations supporting the healing of the burn wounds.
Quay tr li

1. Hydrogel l mt trong nhng nguyn liu dng trong bo ch thuc

phng thi c kim sot.
Thuc phng thi c kim sot l mt trong nhng pht trin trong
ngnh bo ch dc. C 5 dng gii phng thuc:

S phng thi thuc cn p ng c hiu qu tr liu v tc dng trong t chc sng theo nh
gin minh ha:

Vy cc hydrogel ny lm th no to c hiu qu ni trn trong vic kim sot s phng thi

thuc?
Cc hydrogel c pht trin nh l vt liu nhy cm v p ng vi thay i mi trng. Chng
c th thay i th tch p ng theo s thay i cc thng s mi trng nh nit , pH, lc ion,
v.v ( hnh 1). Nhng c tnh c o ca hydrogel em li s quan tm c bit n k thut
vn chuyn dc cht, bao bc t bo v m. Polymer c tnh cht p ng mi trng ng vai
tr quan trng trong s pht trin cc vt liu hydrogel mi.

Vt liu hydrogel c th cu thnh t:

Cc polymer trn hp ( Polymer blends )

S trn hp cc polymer a nc-k nc to ra cc composite hydrogel b phn pha. S trn hp

cc polymer khc nhau v em cc tnh cht ring l ca tng polymer thnh phn vo hn hp
l phng thc r tin v tin li xy dng vt liu mi. Cc polymer trn hp cho cc tnh cht
vt tri khi so vi tnh cht ring r tng thnh phn cng gp li. iu ny c ngha l 1+ 1 >2.
u im ca h polymer trn hp trong ng dng pht thi c kim sot l d s dng trong cc
thit b , d hiu chnh cc tnh cht ca thit b ( hydrat ha, tc gim cp, v bn c hc),
d nng cao tnh cht pht thi thuc v yu t cha thuc v to cc vng cha thuc trong pha

phn tn nh cc vi nang.
Interpolymer
Cc phc hp interpolymer l cc cu trc i phn t hnh thnh bi s lin kt khng ng ha
tr ca cc mch polymer vi nhau. Phc hp ny to thnh t s lin kt cc n v lp li trn
cc mch khc nhau ca cc polymer hoc cc vng ngay chnh trn mch polymer . Phn loi
ch yu ca cc phc hp polymer l phc lp th, phc a in ly, v phc lin kt hydro.
Mng polymer an xuyn (Interpenetrating polymer networks)
Mng polymer an xuyn (IPN) l loi polymer t hp c dng mng li an xen ln nhau.
Trong c t nht mt thnh phn c trng hp , khu mch trong thnh phn khc. Cc IPN
ny bao gm hai hay nhiu polymer trong mng, gi nhau bi cc mc mu t trn hai lin kt
ng ha tr gia cc mch ca hai loi polymer. Cc IPN mang tnh cht vt l tt hn nhiu so
vi cc loi polymer t hp thng thng.
Mng polymer an xuyn (IPN) c phn loi theo ha hc v cu trc:
- Ha hc: IPN hnh thnh tun t, IPN hnh thnh mt ln.
- Cu trc: IPN thun ty, IPN n nht, IPN bn xuyn, IPN nh tng, IPN nhit do

Mt hnh thi c bit ca mng polymer an xuyn l :

cc bn chi polymer

v ht nanopolymer

Copolymer khi (Block copolymers )

Copolymer khi lng tnh c cha cc phn on a nc v k nc lin kt ha hc vi nhau.
Chng em li s a dng hnh thi trong trng thi rn v cc dung mi. Polymer lng in
kiu Zwitter c t nht hai khi mang in tch tri du tng t nh cc protein hnh thnh h
copolymer khi c cc tnh cht c o. Hin nay, cc kiu copolymer khi kiu ABA, ABC mang
c tnh hnh thi dng khi c th cho ra cc hydrogel nhy nhit hiu qu cho ng dng
trong cc h phn phi thuc v liu php t bo.
Teppi, 23 Thng mi mt 2008
#1

2.
TeppiAdministratorStaff Member
[MARQUEE]Vt liu nhy cm v p ng vi thay i mi trng (tip theo)
[/MARQUEE]
Cc cng thc thuc phng thi c kim sot dng qua da, ng ming, s dng hydrogel
kim sot phn phi thuc theo cc c ch sau:

- Khuch tn: khuch tn qua mng bao, khuch tn t trong nn hydrogel

- Gim cp ha hc: nn hydrogel gim cp sinh hc v bo mn sinh hc, thy phn ct lin
kt thuc-hydrogel nh men
- Kch hot bng dung mi: thm thu, trng n
- Thi theo tc ng kch thch ca nhit , pH, in t trng,
Hnh di y l tm tt ba c ch c ng dng ph bin:

Nh vy, cc polymer thnh phn trong hydrogel cn c chn la nh th no c th p

ng mt trong cc c ch phng thi thuc ni trn?

1- Theo c ch khuch tn:

Vic s dng polymer t hp l ph bin khi theo c ch ny.
Polymer t hp gm PVA v chitosan cho mng bao thuc thi theo kiu khuch tn qua mng.
c nhng kt qu ng dng ca thuc thi prostagladin E1, riboflavin
v insulin s dng kiu c ch ny.
Polymer t hp Carbopol 934P v 971P c dng lm hydrogel nn gi cc phn t thuc
nm bn trong. Thuc s khuch tn dn ra ngoi t trong nn polymer t hp ny. Thuc cha
bit dc ibuprofen s dng loi nn ny.

Teppi, 30 Thng mi mt 2008

#2

3.
Turion64Thnh Vin gmLab
2. Theo c ch trng n
S phn tn dc phm theo c ch trng n xut hin khi s khuch tn dc phm/protein
nhanh hn s trng n ca polymer. Do s c s dch chuyn ca cc phn t gia mt phn
cch ca trng thi cao su v trng thi thy tinh ca polymer trng n ging nh s di chuyn
ra vng bin.
3. c ch phn tn dc phm bng phng php ha hc c dng m hnh ha s phn tn
cc phn t, c xc nh thng qua cc phn ng xy ngay trong phn t cht mang. Phn ng

thun ty nht xy ra trong cht mang l phn ng thy phn ca chui polymer, theo con ng
thy phn, s phn hy bi enzym hoc l cc phn ng thun nghch, bt thun nghch xy ra
gia mng li polymer vi dc phm. c ch gim cp ha hc ny chu s chi phi bi cc phn
ng ha hc xy trong xut qu trnh phn tn dc phm. Thng thng s gii phng dc
phm xut hin thng qua s n mn b mt hoc thy phn khi polymer.
Turion64, 23 Thng ba 2009
#3

4.
Turion64Thnh Vin gmLab
Copolymer hydrogel nhy cm nhit
Copolymer nhy cm l nhm vt liu c kh nng t thay i cu trc ca chng cho ph hp vi
s kch thch ca mi trng. C nhiu loi kch thch nhy cm khc nhau nh nhy cm pH,
nhit , in trng
Trong s cc vt liu nhy cm th copolymer hydrogel nhy cm nhit cho thy kh nng chuyn
pha gia sol-gel theo s thay di ca nhit a ra nhng ng dng trong Y sinh nh h
thng phn tn dc phm. Bi v s chuyn pha gia sol-gel ca hydrogel ny c th kim sot
d dng bng s thay i nhit mi trng. Nhng cu trc k nc ca chng tham gia vo
lin kt ca cu trc polymer lm cho dung dch nc ca polymer thay i t trng thi sol sang
gel mt cch d dng di tc dng ca s thay i nhit.
block copolymer dng BAB l mt trong nhng hydrogel nhy cm nhit c trng, chng
cha cc block a nc tm (core) v cc block k nc phn cui cu trc ca polymer.
Nhng block k nc ny ng vai tr nh mt phn nhy cm nhit, tnh k nc ca chng t l
thun vi tng nhit . Do chng th hin c tnh tan- khng tan ca block copolymer
v chng l phn ph thuc vo s thay i nhit .
C ch ca s chuyn pha sol-gel ca copolymer ny nh sau
( file nh km) ( v mnh cch no a hnh vo bi vit )
Cc hydrogel nhy cm nhit nh poly(ethylene oxide)-b-poly(l-lactide-co-glycolide) (PEOP(LLA/GA)), poly(ethylene oxide)-b-poly((D,L-lactide-co-glycolide) (PEO-P(DLLA/GA)), v
PEG-grafted chitosan.
Cc triblock copolymer hydrogel nhy cm nhit khc i t loi cu trc ABA ca poly(ehtylen
glycol)-poly(L-lactide)-poly(thylen glicol) Triblock copolymer (PEG-PLLA-PEG).
Turion64, 23 Thng ba 2009
#4

5.

lazyTThnh Vin gmLab

V vt liu nhy cm v p ng vi mi trng, mnh vn ang thc mc mt s vn c th
sau:
1/ Vt liu nhy nhit: nu a vo c th c nhit n nh t 35 - 37.5 th lm sao tc dng
nhy nhit c pht huy?
2/ Vt liu nhy pH: nu qua ng rut th mnh khng ni, v d dy c acid cao, cc on
rut sau c pH cao hn nn c th khuch tn, thay i trng thi, etc. nhng nu dng qua cch
tim qua da hay truyn trc tip vo mu th c ch tc dng ca VL ny mnh cha hiu lm?
Theo mnh bit th VL ny thng gel ha ngay khi vo c th, vy lm sao vn chuyn c nh?
3/ Ci ny ch l thc mc ngoi l, mnh thy VL nhy cm vi mtruong cn c th c ng dng
lm sensor (tt nhin l biosensor), ko bit nc mnh c ai nghin cu v vn ny khng?
Vi cu hi lm nhm, nhng mong cc AE ch gio. Cm n nhiu!
lazyT, 24 Thng su 2010
#5

6.
lazyTThnh Vin gmLab
Khng bit c AE no gip mnh gii p thc mc v vn ny c ko?
Cm n!
lazyT, 1 Thng by 2010
#6

7.
Turion64Thnh Vin gmLab
V vt liu nhy cm v p ng vi mi trng, mnh vn ang thc mc mt s vn c th
sau:
1/ Vt liu nhy nhit: nu a vo c th c nhit n nh t 35 - 37.5 th lm sao tc dng
nhy nhit c pht huy?
2/ Vt liu nhy pH: nu qua ng rut th mnh khng ni, v d dy c acid cao, cc on
rut sau c pH cao hn nn c th khuch tn, thay i trng thi, etc.
Vt liu nhy cm nhit or/ and pH th hin tc dng nhy cm cc trng thi sau : khi VL
nhit < 35 -37 ( vng nhit c th ) ( v d 15 , pH = 4) thng thng hoc theo mc
ch s dng th n s trng thi lng, nn chng ta c th tim vo c th cch d dng. khi VL
c tim vo c th, nhit mi trng lc ny l 35-37 , pH = 7. VL s chuyn sang trng
thi gel. v n s tn ti trng thi ny trong sut qu trnh phn hy sinh hc ca VL.

nhng nu dng qua cch tim qua da hay truyn trc tip vo mu th c ch tc dng ca VL
ny mnh cha hiu lm? Theo mnh bit th VL ny thng gel ha ngay khi vo c th, vy lm
sao vn chuyn c nh?
Thc t qu trnh gel ha ca VL nhy cm mi trng n khng xy ra tc th , t ngt m n
xy ra sau mt khong thi gian nht nh. do , sau khi c tim vo c th chng c di
chuyn theo ng mu trong ng mch or tnh mch n mt vng cn cha tr ( v d nh
khi u, t bo ung th ) th tp trung li v sau mt khong thi gian chng s gel ha v li ti
vng . thc hin qu trnh phn hy sinh hc thi thuc /protein khng ch t bo ung th.
ci ny ngi ta gi l thuc ti ch.
Cn trng hp tim vi di da, sau khi tim vo c th mt thi gian th VL s gel ha v ti
v tr tim. ti VL ngm thuc s thc hin qu trnh phn hy sinh hc thi thuc/protein.
thuc/protein s theo ng mu i n cc vng cn cha tr thc hin nhim v.
Hin ti VN c mt s bo co v VL nhy cm mi trng, nu Bn quan tm c th lin h
Phng TN trng im Quc Gia Polymer & Composite, i hc BK HCM.
Turion64, 4 Thng tm 2010
#7

8.
TeppiAdministratorStaff Member
lazyT said:
V vt liu nhy cm v p ng vi mi trng, mnh vn ang thc mc mt s vn c th sau:
1/ Vt liu nhy nhit: nu a vo c th c nhit n nh t 35 - 37.5 th lm sao tc dng nhy nhit c
pht huy?
2/ Vt liu nhy pH: nu qua ng rut th mnh khng ni, v d dy c acid cao, cc on rut sau c pH cao
hn nn c th khuch tn, thay i trng thi, etc. nhng nu dng qua cch tim qua da hay truyn trc tip vo
mu th c ch tc dng ca VL ny mnh cha hiu lm? Theo mnh bit th VL ny thng gel ha ngay khi vo c
th, vy lm sao vn chuyn c nh?
3/ Ci ny ch l thc mc ngoi l, mnh thy VL nhy cm vi mtruong cn c th c ng dng lm sensor (tt
nhin l biosensor), ko bit nc mnh c ai nghin cu v vn ny khng?
Vi cu hi lm nhm, nhng mong cc AE ch gio. Cm n nhiu!
Click to expand...

Bn lazy thn mn,

Cu hi ca bn c bn Turion gii p mt phn, ti b sung thm bn nm r hn.
2/ Vt liu nhy pH: nu qua ng rut th mnh khng ni, v d dy c acid cao, cc on
rut sau c pH cao hn nn c th khuch tn, thay i trng thi, etc. nhng nu dng qua cch
tim qua da hay truyn trc tip vo mu th c ch tc dng ca VL ny mnh cha hiu lm?
Theo mnh bit th VL ny thng gel ha ngay khi vo c th, vy lm sao vn chuyn c nh?
Nu dng vt liu nhy pH cho thuc tim qua da s9it theo ng mu th n phi p ng theo
c ch lng pH trung tnh, gel pH thp nu theo ti vng thn, phi. Thut t gel y ch l
mt phn, ch ng ra l "bm ln " m hoc t bo bnh. Trng hp ny, vt liu nhy pH ng

vai tr nh cht liu tng bm ch khng phi l ti thuc hon ton.

Trng hp ny dng trong chin thut "gy i" t bo bnh bng cch bao vy bng mng ph
gel, khng cho chng tip xc vi cc phn t ng...
Mt s cch khc nh thc hin lp 2-3 mng vt liu nhy pH cao kt hp nhy pH thp. Lp th
nht, ph ngoi b gel trong pH cao. Khi vt liu ti vng pH thp, lp ph ngoi tan l lp
trong nhy pH thp. Nh vy thuc cha trong nn 2 lp ny c ri u t vng pH cao ti
thp.
1-Vt liu nhy nhit: nu a vo c th c nhit n nh t 35 - 37.5 th lm sao tc dng
nhy nhit c pht huy?
Vt liu nhy nhit khng nht thit ch nhy vng nhit 35-37 C. V d, vt liu nhy nhit
ni sinh dng trong trng hp chiu sng vi ba cng thp ln t bo bnh. Vt liu ny l
sn phm polymer gn cc gc ng v gc nhy sng vi ba. T bo bnh hp th n dng lng
trong iu kin nhit c th. Khi b chiu bc x vi ba, bo tng t bo nng ln, cht liu
phn hy gii phng cc gc tiu dit t bo bnh ( hoc gel ha gy c ch trao i cht bn
trong t bo bnh) v chuyn sang trung tnh khi tip xc t bo lnh.
Thn,