Republic of the Philippines

BATANGAS STATE UNIVERSITY

Pablo Borbon Main II
Batangas City

COLLEGE OF ENGINEERING, ARCHITECTURE and FINE ARTS

Civil and Sanitary Engineering Department

SE 404
Microbiology and Parasitology for Environmental
Engineering

Laboratory Activity No. 2

Algae & Fungi Observation

Group No. 8

Castillo, Kathleen Kaye D.

Ilagan, Pia Donna O.
Lat, Lady Anne Aira M.
Lorena, Rhona Jane A.
Marquez, Jaysha Mae D.

BSSE 2201

Date
 Algae & Fungi Observation

OBJECTIVES
 Learn to collect and culture algae and fungi
 Learn to recognize and describe the general characteristics of algae and fungi
 To observe and identify the various structures of each algae and fungi

THEOREM


MATERIALS USED

Microscope Tweezer Syringe

3 Microscopic Slides & Cover

Container Slip Water

Alcohol Tissue

Specimen
 Algae Green Seaweeds Brown Seaweeds

Bread mold Mushroom

Procedure
1. Prepare the materials needed and collect the specimen to be examined.
2. Connect your compound light microscope to an outlet.
3. Get the slide and place the specimen to be tested on the central part of the slide. Use
cover slip if necessary.
4. Place a drop of water for the selected specimen in the center of the slide, using a
syringe if you have one, or the tip of a clean finger.
5. Take the cover slip and set it at an angle to the slide so that one edge of it touches the
water drop, then carefully lower it over the drop so that the cover slip covers the
specimen without trapping air bubbles underneath.
6. Use the corner of a tissue to blot up any excess water at the edges of the cover slip.
7. Place the slide onto the stage using its metal clips.
8. View the slide with a compound microscope, starting with a low objective.
9. Adjust the coarse (focus) and fine (sharpen) adjustment knobs until you view the
image clearly.
10. Examine your specimen by their structures and characteristics.
11. Capture the examined specimen for your laboratory report.
12. When you’re done examining your specimen, turn the objectives lens knob until it is at
the highest point from the specimen. Turn the nosepiece back to the lowest power lens,
carefully remove the slide, and place a cover on your microscope.
13. Clean-up: When the experiment is finished, put the bread and anything that touched it
in a heavy-duty plastic bag, and throw it away. The slide will not be permanent, and
should be disposed of as well. Clean the area you were working in thoroughly.

Data Presentation and Analysis

Specimen Objective Analysis
Lenses Used
 This green alga which comes from the
phyla chlorophyta is a multicellular and
is found in the vicinity of water pipes.
Its predominant color is green and has a
4 x .10 photosynthetic pigments of chlorophyll
b and carotenoids.

Algae
These green seaweeds which are type of
chlorophyta are unicellular or simple
filaments, and are found in the ocean. Its
predominant color is green and has a
4 x .10 photosynthetic pigments of chlorophyll
b and carotenoids.

Green Seaweeds
These brown seaweeds or called
sargassum comes from the phyla of
phaeophyta which are multicellular
seaweeds. Its predominant color is olive
4 x .10 brown and has photosynthetic pigments
of chlorophyll c, carotenoids and
xanthophylls.

Brown Seaweeds

This bread mold comes from the type of

phyla Ascomycotina which has a septate
type of hyphae. Its sexual spore is called
4 x .10 ascospore. On the other hand, its type of
asexual spore is called conidiospore.

Bread mold
These mushroom is a class of true fungi
that consist of a true network of
filaments or strands which was found in
a rotting log that forms and releases
spores. The spores are much like the
4 x .10 seed of a plant just like what is shown in
the picture. The type of their sexual
spore is called basidiospore. These
fleshy fungi comes from the phyla of
basidiomycotina which has a septate
Mushroom type of hyphae.

Results and Discussion


Conclusion
In conclusion, algae are eukaryotic and photosynthetic organisms. Algae can be either
unicellular or multicellular organisms. They use carbon dioxide as an energy source, the
reason why algae are on or near the ocean. Reproduction in algae occurs in both asexual and
sexual methods. Asexual reproduction occurs by spore formation. Sexual reproduction
occurs by the physical contact of haploid gametes.
Like terrestrial plants, all types of seaweed use sunlight, carbon dioxide and water to
create food. For this reason, seaweed must grow near the ocean's surface within the reach of
sunlight to survive, and there must be an abundance of carbon dioxide in the water.

Encountered Problems



Recommendations



Distribution of Works




Documentations

SE 404 – Microbiology and Parasitology for Environmental Engineering

Rubrics for Laboratory Activity Reports
 EXEMPLARY COMPETENT NEEDS IMPROVEMENT
CRITERIA
85% – 100% 75% - 84% 74% and below
Laboratory experiment’s
Laboratory experiment’s
Laboratory experiment’s objectives are stated but one or
objectives are not stated
OBJECTIVES objectives are stated clearly both are not as clear as they
clearly and the relationship
(15%) and the relationship between might be, or the relationship
between the objectives is
the objectives is clear. between the objectives is
unclear or absent.
unclear.
The procedure is well The procedure could be more The procedure is not clearly
PROCEDURE designed. All stages of the efficiently designed. Most designed. Many stages of the
(15%) procedure are entered on the stages of the procedure are procedure are not entered on
lab report. entered on the lab report. the lab report.
Important theories are stated Important theories are stated
Important theories are not
THEOREM clearly and imply its clearly but do not imply its
stated on the lab report of the
(15%) significance on the experiment significance on the experiment
experiment conducted.
conducted. conducted.

Data are presented in ways

DATA Data are presented in ways
PRESENTATION & (charts, tables, graphs) that
ANALYSIS best facilitate understanding
(25%) and interpretation.
The results are fully
interpreted and compared with
EVALUATION OF literature values. The
EXPERIMENT limitations and weaknesses are
(20%) discussed and suggestions are
made as to how to limit or
eliminate them.
The Laboratory Report
COMPLETENESS &
contains all the necessary parts
NEATNESS
and it is presented completely
(10%)
and neatly.
(charts, tables, graphs) that
can be understood and
interpreted, although not as
clearly as they might be.
The results are interpreted and
compared with literature
values, but not as fully as they
might be. The limitations and
weaknesses are discussed, but
few or no suggestions are
made as to how to limit or
eliminate them.
The Laboratory Report
contains some of the necessary
parts and it is presented quiet
complete and neat.
Data are presented in ways
(charts, tables, graphs) that are
very unclear.
The results are not interpreted
in a logical way or compared
with literature values. The
limitations and weaknesses are
not discussed, nor are
suggestions made as to how to
limit or eliminate them.
The Laboratory Report do not
contains all the necessary parts
and it is not presented
completely and neatly.

Laboratory Activity No.2

Title : Fungi & Algae Observation

Group No. 8 Section : BSSE-2101

Group Members
1. Castillo, Kathleen Kaye D. 3. Lat, Lady Anne Aira M.
2. Ilagan, Pia Donna O. 4. Lorena, Rhona Jane A.
5. Marquez, Jaysha Mae D.
CRITERIA % Grade (%) Weighted Grade

Objectives 15

Procedure 15

Theorem 15

Data Presentation & Analysis 25

Evaluation Of Experiment 20

Completeness & Neatness 10

F I N A L G R A D E