6 tubes containing starch and iodine at temperature at 0⁰C, 37⁰C, 100⁰C

Figure 2 : 3 tubes containing iodine and starch at pH 4, 7, 10

Temperatur Tube Presence of starch (+/-) Presence of starch ( Total of “+”)

e No
0C 1 ++ ++
2 ++
37 C 1 + +
2 +
100 C 1 +++ +++
2 ++++
Table 1 : Effect of temperature to enzyme
 pH Presence of starch (+/-)
4 ++
7 +
10 ++
Table 2 : Effect of pH to enzyme