‫ﻣﺘﺮﺟﻢ ﻣﻦ ﺍﻹﻧﺠﻠﻴﺰﻳﺔ ﺇﻟﻰ ﺍﻟﻌﺮﺑﻴﺔ ‪www.onlinedoctranslator.

com -‬‬

‫™‬ ‫ﻡ‪Endo Broth MF‬‬

‫ﻣﻌﺎﺩﻟﺔ‬
‫ﺍﻻﺳﺘﺨﺪﺍﻡﺍﻟﻤﻘﺼﻮﺩ‬
‫ﺩﻳﻔﻜﻮ™ﻡ ‪Endo Broth MF‬‬
‫ﻡ‪*™Endo Broth MF‬ﻳﺴﺘﺨﺪﻡ ﻟﺘﻌﺪﺍﺩ ﺍﻟﻜﺎﺉﻨﺎﺕ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﻓﻲ‬
‫™‬

‫ﺍﻟﺼﻴﻐﺔﺍﻟﺘﻘﺮﻳﺒﻴﺔ * ﻟﻜﻞ ﻟﺘﺮ‬

‫ﺯ‬ ‫ﺧﻼﺻﺔﺍﻟﺨﻤﻴﺮﺓ ‪1.5 .............. ................................................‬‬
‫ﺯ‬ ‫‪Casitone.................................. ................................... 5.0‬‬
‫ﺯ‬ ‫ﺛﻴﻮﺑﺒﺘﻮﻥ ‪5.0.................................................. ...‬‬
‫ﺯ‬ ‫ﺗﺮﻳﺒﺘﻮﺯ ‪10.0.................... ................................................‬‬
‫ﺯ‬ ‫ﺍﻟﻼﻛﺘﻮﺯ ‪12.5......................................... ............................‬‬
‫ﺯ‬ ‫ﺩﻳﻮﻛﺴﻮﻛﺴﻴﻜﻮﻻﺕﺍﻟﺼﻮﺩﻳﻮﻡ ‪0.1 .......................................... ......‬‬
‫ﻓﻮﺳﻔﺎﺕﺛﻨﺎﺉﻲ ﺑﻮﺗﺎﺳﻴﻮﻡ ‪ 4.375 .......................................... .....‬ﺟﻢ‬
‫ﻓﻮﺳﻔﺎﺕﺑﻮﺗﺎﺳﻴﻮﻡ ﺃﺣﺎﺩﻱ ‪ 1.375 ..................................... ...‬ﺟﻢ ﻛﻠﻮﺭﻳﺪ‬
‫ﺍﻟﺼﻮﺩﻳﻮﻡ ‪ 5.0................................................ .........‬ﺻﻮﺩﻳﻮﻡ ﻛﺒﺮﻳﺘﺎﺕﺯ‬
‫ﺯ‬ ‫ﻟﻮﺭﻳﻞ ‪ 0.05.. ................................................‬ﻛﺒﺮﻳﺘﻴﺖ‬
‫ﺯ‬ ‫ﺍﻟﺼﻮﺩﻳﻮﻡ ‪Fuchsin 2.1................ .............................................‬‬
‫ﺯ‬ ‫ﺍﻷﺳﺎﺳﻲ ‪1.05...................... ...‬‬
‫* ﻣﻌﺪﻟﺔ ﻭ ‪ /‬ﺃﻭ ﻣﻜﻤﻠﺔ ﺣﺴﺐ ﺍﻻﻗﺘﻀﺎء ﻟﺘﻠﺒﻴﺔ ﻣﻌﺎﻳﻴﺮ ﺍﻷﺩﺍء‪.‬‬
‫‪ m Endo‬ﻓﻲ ﺇﺟﺮﺍء ﺍﻟﺘﺮﺷﻴﺢ ﺍﻟﻘﻴﺎﺳﻲ ﻟﻐﺸﺎء ﺍﻟﻘﻮﻟﻮﻥ ﺍﻟﻜﻠﻲ ﻻﺧﺘﺒﺎﺭ‬
‫ﺍﺗﺠﺎﻫﺎﺕﺍﻟﺘﺤﻀﻴﺮ ﻣﻦ ﺍﻟﻤﻨﺘﺞ‬
‫ﺍﻟﻤﺠﻔﻒ‬
‫‪.1‬ﻗﻢ ﺑﺈﺫﺍﺑﺔ ‪ 48‬ﺟﻢ ﻣﻦ ﺍﻟﻤﺴﺤﻮﻕ ﻓﻲ ‪ 1‬ﻟﺘﺮ ﻣﻦ ﺍﻟﻤﺎء ﺍﻟﻨﻘﻲ‬
‫ﺍﻟﻤﺤﺘﻮﻱﻋﻠﻰ ‪ 20‬ﻣﻞ ﻣﻦ ﺍﻹﻳﺜﺎﻧﻮﻝ ﻏﻴﺮ ﺍﻟﻤﻌﺎﻟﺞ‪.‬‬
‫‪.2‬ﺗﺴﺨﻴﻨﻬﺎ ﺣﺘﻰ ﺍﻟﻐﻠﻴﺎﻥ ﻭﻟﻜﻦ ﺗﺠﻨﺐ ﺍﻹﻓﺮﺍﻁ ﻓﻲ ﺍﻟﺘﺴﺨﻴﻦ‪ .‬ﻻ‬
‫ﺗﻐﻠﻖﺗﻠﻘﺎﺉﻲ‪.‬‬
‫‪.3‬ﺍﺧﺘﺒﺎﺭ ﻋﻴﻨﺎﺕ ﻣﻦ ﺍﻟﻤﻨﺘﺞ ﺍﻟﻨﻬﺎﺉﻲ ﻟﻸﺩﺍء ﺑﺎﺳﺘﺨﺪﺍﻡ ﺛﻘﺎﻓﺎﺕ ﺗﺤﻜﻢ‬
‫ﻧﻤﻮﺫﺟﻴﺔﻣﺴﺘﻘﺮﺓ‪.‬‬
‫ﺗﻨﺒﻴﻪ‪:‬ﺍﺣﻔﻈﻪ ﻣﻦ ﺍﻟﻀﻮء‪ .‬ﺍﺳﺘﺨﺪﻡ ﻋﻠﻰ ﺍﻟﻔﻮﺭ‪.‬‬
‫ﻳﺤﺘﻮﻱﻣﺮﻕ ﺇﻧﺪﻭ ﺇﻡ ﺇﻑ ﻋﻠﻰ ﺍﻟﺒﻴﺒﺘﻮﻥ ﻛﻤﺼﺎﺩﺭ ﻟﻠﻜﺮﺑﻮﻥ ﻭﺍﻟﻨﻴﺘﺮﻭﺟﻴﻦ‬
‫ﻭﺍﻟﻔﻴﺘﺎﻣﻴﻨﺎﺕﻭﺍﻟﻤﻌﺎﺩﻥ‪ .‬ﻳﻮﻓﺮ ﻣﺴﺘﺨﻠﺺ ﺍﻟﺨﻤﻴﺮﺓ ﻓﻴﺘﺎﻣﻴﻨﺎﺕ ﺏ‬
‫ﺇﺟﺮﺍء‬
‫‪.1‬ﺿﻊ ﻭﺳﺎﺩﺓ ﻣﺎﺻﺔ ﻟﻤﺮﺷﺢ ﻏﺸﺎﺉﻲ ﺩﺍﺧﻞ ﻃﺒﻖ ﺑﺘﺮﻱ ﻣﻌﻘﻢ ‪60‬‬
‫ﻣﻢ‪.‬‬
‫‪.2‬ﺃﺿﻒ ‪ 2.0-1.8‬ﻣﻞ ﻣﻦ ‪ Endo Broth MF‬ﻟﻜﻞ ﺿﻤﺎﺩﺓ‪.‬‬
‫‪.3‬ﺗﺼﻔﻴﺔ ﻋﻴﻨﺔ ﺍﻟﻤﻴﺎﻩ ﻣﻦ ﺧﻼﻝ ﻣﺮﺷﺢ ﻏﺸﺎﺉﻲ‪.‬‬
‫‪.4‬ﺿﻊ ﺟﺎﻧﺐ ﺍﻟﻤﺮﺷﺢ ﺍﻟﻌﻠﻮﻱ ﻷﻋﻠﻰ ﻋﻠﻰ ﺍﻟﻮﺳﺎﺩﺓ ﺑﺎﺳﺘﺨﺪﺍﻡ ﺣﺮﻛﺔ ﺩﺍﺉﺮﻳﺔ‬
‫ﻟﺘﺠﻨﺐﻓﻘﺎﻋﺎﺕ ﺍﻟﻬﻮﺍء ﺍﻟﻤﺤﺎﺻﺮﺓ‪.‬‬
‫‪.5‬ﺍﻗﻠﺐ ﺍﻟﻄﺒﻖ ﻭﺍﺣﺘﻀﻨﻪ ﻟﻤﺪﺓ ‪ 24-22‬ﺳﺎﻋﺔ ﻋﻨﺪ ‪ 0.5 ± 35‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ‪.‬‬
‫‪.6‬ﺭﺍﻗﺐ ﻭﻋﺪ ﺟﻤﻴﻊ ﺍﻟﻤﺴﺘﻌﻤﺮﺍﺕ ﺫﺍﺕ ﺍﻟﻠﻮﻥ ﺍﻷﺣﻤﺮ ﻭﺍﻟﺘﻲ ﻟﻬﺎ‬
‫ﻟﻤﻌﺎﻥﻣﻌﺪﻧﻲ‪.‬‬
‫ﻧﺘﺎﺉﺞﻣﺘﻮﻗﻌﺔ‬
‫ﺗﻌﺘﺒﺮﺟﻤﻴﻊ ﺍﻟﻤﺴﺘﻌﻤﺮﺍﺕ ﺫﺍﺕ ﺍﻟﻠﻮﻥ ﺍﻷﺣﻤﺮ ﻭﺍﻟﺘﻲ ﻟﻬﺎ ﻟﻤﻌﺎﻥ‬
‫ﻣﻌﺪﻧﻲﻣﻤﻴﺰ ﻣﻦ ﺍﻟﻘﻮﻟﻮﻧﻴﺎﺕ‪ .‬ﻗﺪ ﻳﻐﻄﻲ ﺍﻟﻠﻤﻌﺎﻥ ﺍﻟﻤﺴﺘﻌﻤﺮﺓ‬
‫ﺑﺄﻛﻤﻠﻬﺎ ‪،‬ﻭﻗﺪ ﻳﻜﻮﻥ ﻓﻘﻂ ﻓﻲ ﺍﻟﻤﻨﺘﺼﻒ ﺃﻭ ﻗﺪ ﻳﻈﻬﺮ ﻓﻘﻂ ﺣﻮﻝ‬
‫ﺍﻟﺤﻮﺍﻑ‪.‬‬
‫ﺍﻟﻤﺎءﻋﻦ ﻃﺮﻳﻖ ﺍﻟﺘﺮﺷﻴﺢ ﺍﻟﻐﺸﺎﺉﻲ‪.‬‬
‫‪ Millipore Corporation.‬ﻫﻲ ﻋﻼﻣﺔ ﺗﺠﺎﺭﻳﺔ ﻟﺸﺮﻛﺔ ‪* MF‬‬
‫ﺍﻟﻤﻠﺨﺺﻭﺍﻟﺸﺮﺡ‬
‫ﺗﻢﺗﺤﻀﻴﺮ ﻣﺮﻕ ‪ Endo Broth MF‬ﻭﻓﻘﺎً ﻟﺘﺮﻛﻴﺒﺔ ﺷﺮﻛﺔ‬
‫‪1Millipore Corporation‬ﻟﻌﺰﻝ ﺍﻟﺒﻜﺘﻴﺮﻳﺎ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﺑﺸﻜﻞ‬
‫ﺍﻧﺘﻘﺎﺉﻲﻣﻦ ﺍﻟﻤﺎء ﻭﺍﻟﻌﻴﻨﺎﺕ ﺍﻷﺧﺮﻯ ﺑﺎﺳﺘﺨﺪﺍﻡ ﺗﻘﻨﻴﺔ ﺍﻟﺘﺮﺷﻴﺢ‬
‫ﺍﻟﻐﺸﺎﺉﻲ‪.‬ﺍﻟﻮﺳﻂ ﻋﺒﺎﺭﺓ ﻋﻦ ﻣﺰﻳﺞ ﻣﻦ ﻣﺮﻕ ‪HD Endo Medium‬‬
‫‪ m‬ﺍﻟﺴﺎﺑﻖ ﻭ ‪.Lauryl Tryptose Broth‬‬
‫ﺗﺤﺪﺩﺟﻤﻌﻴﺔ ﺍﻟﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ )‪ (APHA‬ﺍﺳﺘﺨﺪﺍﻡ ‪Broth MF‬‬
‫ﺍﻟﻤﻴﺎﻩ‪2‬ﻭﺍﻟﻤﻴﺎﻩ ﺍﻟﻤﻌﺒﺄﺓ ﻓﻲ ﺯﺟﺎﺟﺎﺕ‪3.‬ﺗﺤﺪﺩ ‪ APHA‬ﺃﻳﻀﺎً ﺍﺳﺘﺨﺪﺍﻡ‬
‫‪ m Endo Broth MF‬ﻓﻲ ﺍﻹﺟﺮﺍء ﺍﻟﻘﻮﻟﻮﻧﻲ ﺍﻟﻜﻠﻲ ﺍﻟﻤﺘﺄﺧﺮ ﻟﻠﺤﻀﺎﻧﺔ‬
‫ﻋﻦﻃﺮﻳﻖ ﺇﺿﺎﻓﺔ ﺑﻨﺰﻭﺍﺕ ﺍﻟﺼﻮﺩﻳﻮﻡ ﻟﺼﻨﻊ ﻭﺳﻂ ﺣﺎﻓﻈﺔ ‪2.Endo‬ﻳﺘﻢ‬
‫ﺗﻌﺮﻳﻒﺍﻟﺒﻜﺘﻴﺮﻳﺎ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﻋﻠﻰ ﺃﻧﻬﺎ ﺑﻜﺘﻴﺮﻳﺎ ﺗﻨﺘﺞ ﻣﺴﺘﻌﻤﺮﺓ ﺣﻤﺮﺍء‬
‫ﺫﺍﺕﻟﻤﻌﺎﻥ ﻣﻌﺪﻧﻲ ﺧﻼﻝ ‪ 24‬ﺳﺎﻋﺔ ﻣﻦ ﺍﻟﺤﻀﺎﻧﺔ ﻋﻨﺪ ‪ 35‬ﺩﺭﺟﺔ‬
‫ﻣﺉﻮﻳﺔﻋﻠﻰ ﻭﺳﻂ ﻣﻦ ﺍﻟﻨﻮﻉ ‪.Endo‬‬
‫ﻣﺒﺎﺩﺉﺍﻹﺟﺮﺍء‬
‫ﺍﻟﻤﻌﻘﺪﺓ ‪،‬ﻭﺍﻟﺘﻲ ﺗﺤﻔﺰ ﻧﻤﻮ ﺍﻟﺒﻜﺘﻴﺮﻳﺎ‪ .‬ﺍﻟﻼﻛﺘﻮﺯ ﻫﻮ ﺍﻟﻜﺮﺑﻮﻫﻴﺪﺭﺍﺕ‪.‬‬
‫ﺍﻟﻔﻮﺳﻔﺎﺕﻋﻮﺍﻣﻞ ﺍﻟﺘﺨﺰﻳﻦ ﺍﻟﻤﺆﻗﺖ‪ .‬ﻳﺤﺎﻓﻆ ﻛﻠﻮﺭﻳﺪ ﺍﻟﺼﻮﺩﻳﻮﻡ ﻋﻠﻰ‬
‫ﺍﻟﺘﻮﺍﺯﻥﺍﻷﺳﻤﻮﺯﻱ ﻟﻠﻮﺳﻂ‪ .‬ﻳﻀﺎﻑ ﺩﻳﻮﻛﺴﻴﻜﻮﻻﺕ ﺍﻟﺼﻮﺩﻳﻮﻡ‬
‫ﻭﻛﺒﺮﻳﺘﺎﺕﻟﻮﺭﻳﻞ ﺍﻟﺼﻮﺩﻳﻮﻡ ﻛﻤﺜﺒﻄﺎﺕ ﻟﻠﺒﻜﺘﻴﺮﻳﺎ ﻣﻮﺟﺒﺔ ﺍﻟﺠﺮﺍﻡ‪.‬‬
‫‪ fuchsin‬ﺍﻷﺳﺎﺳﻲ ﻫﻮ ﻣﺆﺷﺮ ﺍﻷﺱ ﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ‪ .‬ﻳﻀﺎﻑ ﻛﺒﺮﻳﺘﻴﺖ‬
‫ﺍﻟﺼﻮﺩﻳﻮﻡﻹﺯﺍﻟﺔ ﻟﻮﻥ ﻣﺤﻠﻮﻝ ﺍﻟﻔﻮﻛﺴﻴﻦ ﺍﻷﺳﺎﺳﻲ‪ .‬ﺗﺰﻳﺪ ﻣﺎﺩﺓ‬
‫ﺍﻹﻳﺜﺎﻧﻮﻝﺍﻟﻤﻀﺎﻓﺔ ﻣﻦ ﺍﻟﻄﺒﻴﻌﺔ ﺍﻟﻤﻀﺎﺩﺓ ﻟﻠﺒﻜﺘﻴﺮﻳﺎ ﻟﻠﺘﺮﻛﻴﺒﺔ‪.‬‬
‫ﺗﻨﺘﺞﺍﻟﺒﻜﺘﻴﺮﻳﺎ ﺍﻟﻤﺨﻤﺮﺓ ﻟﻼﻛﺘﻮﺯ ﺃﺳﻴﺘﺎﻟﺪﻳﻬﻴﺪ ﺍﻟﺬﻱ ﻳﺘﻔﺎﻋﻞ ﻣﻊ‬
‫ﻛﺒﺮﻳﺘﻴﺖﺍﻟﺼﻮﺩﻳﻮﻡ ﻭﺍﻟﻔﻮﻛﺴﻴﻦ ﻟﺘﺸﻜﻴﻞ ﻣﺴﺘﻌﻤﺮﺍﺕ ﺣﻤﺮﺍء‪.‬‬
‫ﻳﺤﺪﺙﺗﻄﻮﺭ ﻟﻤﻌﺎﻥ ﻣﻌﺪﻧﻲ ﻋﻨﺪﻣﺎ ﻳﻨﺘﺞ ﺍﻟﻜﺎﺉﻦ ﺍﻟﺤﻲ ﺍﻷﻟﺪﻫﻴﺪﺍﺕ‬
‫ﻣﻊﺍﻟﺘﺨﻤﻴﺮ ﺍﻟﺴﺮﻳﻊ ﻟﻼﻛﺘﻮﺯ‪ .‬ﺇﺫﺍ ﻛﺎﻧﺖ ﺍﻟﻠﻘﺎﺡ ﺛﻘﻴﻼً ﺟﺪﺍً ‪ ،‬ﻓﺴﻴﺘﻢ‬
‫ﺇﺧﻤﺎﺩﺍﻟﻠﻤﻌﺎﻥ‪ .‬ﺗﺸﻜﻞ ﺍﻟﺒﻜﺘﻴﺮﻳﺎ ﻏﻴﺮ ﺍﻟﻤﺨﻤﺮﺓ ﻟﻼﻛﺘﻮﺯ ﻣﺴﺘﻌﻤﺮﺍﺕ‬
‫ﻭﺍﺿﺤﺔﻋﺪﻳﻤﺔ ﺍﻟﻠﻮﻥ‪.‬‬

‫ﺣﺪﻭﺩﺍﻹﺟﺮﺍء‬
‫ﻣﻦﺣﻴﻦ ﻵﺧﺮ ‪ ،‬ﻗﺪ ﺗﻨﺘﺞ ﺍﻟﻜﺎﺉﻨﺎﺕ ﻏﻴﺮ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﻣﺴﺘﻌﻤﺮﺍﺕ‬
‫ﻟﻤﻌﺎﻥﻧﻤﻮﺫﺟﻴﺔ‪ .‬ﻗﺪ ﺗﻨﺘﺞ ﺍﻟﻜﺎﺉﻨﺎﺕ ﺍﻟﻘﻮﻟﻮﻧﻴﺔ ﺃﺣﻴﺎﻧﺎً ﻣﺴﺘﻌﻤﺮﺍﺕ‬
‫ﻏﻴﺮﻧﻤﻄﻴﺔ )ﺣﻤﺮﺍء ﺩﺍﻛﻨﺔ ﺃﻭ ﻣﺴﺘﻌﻤﺮﺍﺕ ﻧﻮﺍﺓ ﺑﺪﻭﻥ ﻟﻤﻌﺎﻥ(‪ .‬ﻣﻦ‬
‫ﺍﻟﻤﺴﺘﺤﺴﻦﺍﻟﺘﺤﻘﻖ ﻣﻦ ﻛﻼ ﻧﻮﻋﻲ ﺍﻟﻤﺴﺘﻌﻤﺮﺍﺕ‪2.‬‬

‫ﺍﻹﺻﺪﺍﺭﺍﻟﺜﺎﻧﻲ ‪Difco ™ & BBL ™ Manual ،‬‬

 ‫ﻣﺮﺍﺟﻊ‬ ‫ﻣﺮﺍﻗﺒﺔﺟﻮﺩﺓ ﺍﻟﻤﺴﺘﺨﺪﻡ‬
‫‪.‬ﺟﻤﻌﻴﺔﺃﻋﻤﺎﻝ ﺍﻟﻤﻴﺎﻩ ‪ Schaufus. 1958. J. Am.‬ﻭ ‪.193 :501. Fifield‬‬
‫‪.2‬ﺇﻳﺘﻮﻥ ﻭﺭﺍﻳﺲ ﻭﺑﻴﺮﺩ )ﻣﺤﺮﺭ(‪ .2005 .‬ﺍﻟﻄﺮﻕ ﺍﻟﻘﻴﺎﺳﻴﺔ ﻟﻔﺤﺺ ﺍﻟﻤﻴﺎﻩ ﻭﺍﻟﺼﺮﻑ ﺍﻟﺼﺤﻲ ‪ ،‬ﺍﻟﻄﺒﻌﺔ ‪ ، 21‬ﻋﺒﺮ‬
‫ﺍﻹﻧﺘﺮﻧﺖ‪.‬ﺍﻟﺮﺍﺑﻄﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ ﻟﻠﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ‪ ،‬ﻭﺍﺷﻨﻄﻦ ﺍﻟﻌﺎﺻﻤﺔ‬
‫ﻣﻮﺍﺻﻔﺎﺕﺍﻟﻬﻮﻳﺔ‬
‫‪.3‬ﻛﻴﻢ ﻭﻓﻨﻎ‪.2001 .‬ﻓﻲﺩﺍﻭﻧﺰ ﻭﺇﻳﺘﻮ )ﻣﺤﺮﺭ( ‪ ،‬ﺧﻼﺻﺔ ﻭﺍﻓﻴﺔ ﻟﻄﺮﻕ ﺍﻟﻔﺤﺺ ﺍﻟﻤﻴﻜﺮﻭﺑﻴﻮﻟﻮﺟﻲ ﻟﻸﻃﻌﻤﺔ ‪ ،‬ﺍﻟﻄﺒﻌﺔ‬ ‫™‬ ‫ﺩﻳﻔﻜﻮ™ﻡ ‪Endo Broth MF‬‬
‫ﺍﻟﺮﺍﺑﻌﺔ‪.‬ﺍﻟﺮﺍﺑﻄﺔ ﺍﻷﻣﺮﻳﻜﻴﺔ ﻟﻠﺼﺤﺔ ﺍﻟﻌﺎﻣﺔ ‪ ،‬ﻭﺍﺷﻨﻄﻦ ﺍﻟﻌﺎﺻﻤﺔ‬
‫ﺃﺭﺟﻮﺍﻧﻲﻭﺭﺩﻱ ‪ ،‬ﻳﺘﺪﻓﻖ ﺑﺤﺮﻳﺔ ‪ ،‬ﻣﺘﺠﺎﻧﺲ‪.‬‬ ‫ﻣﻈﻬﺮﺟﺎﻑ‪:‬‬
‫ﻣﺤﻠﻮﻝ‪ ، ٪4.8‬ﻗﺎﺑﻞ ﻟﻠﺬﻭﺑﺎﻥ ﺟﺰﺉﻴﺎ ًﻓﻲ ﻣﺎء ﻧﻘﻲ‬ ‫ﺣﻞ‪:‬‬
‫ﺍﻟﺘﻮﻓﺮ‬ ‫ﻳﺤﺘﻮﻱﻋﻠﻰ ‪ ٪2‬ﺇﻳﺜﺎﻧﻮﻝ ﻋﻨﺪ ﺍﻟﻐﻠﻴﺎﻥ‪ .‬ﺍﻟﻤﺤﻠﻮﻝ‬
‫™‬‫ﺩﻳﻔﻜﻮ™ﻡ ‪Endo Broth MF‬‬ ‫ﺃﺣﻤﺮﻣﺎﺉﻞ ﻟﻠﻮﺭﺩﻱ ‪ ،‬ﺑﺮﺍﻕ ﻣﻊ ﺗﺮﺳﺐ‪.‬‬
‫‪SMD SMWW‬‬ ‫‪COMPF‬‬ ‫ﺃﺣﻤﺮﻭﺭﺩﻱ ‪ ،‬ﺑﺮﺍﻕ ﻣﻊ ﺗﺮﺳﺒﺎﺕ‪.‬‬ ‫ﺍﻟﻤﻈﻬﺮﺍﻟﺠﺎﻫﺰ‪:‬‬
‫ﻣﺠﻔﻔﺔ‪ 100 -‬ﻍ ﻣﺠﻔﻔﺔ‬ ‫‪274920‬‬ ‫ﻗﻄﺔ‪.‬ﻻ‪.‬‬ ‫ﺭﺩﻓﻌﻞ ‪٪4.8‬‬
‫‪ 500-‬ﻍ‬ ‫‪274930‬‬ ‫ﺍﻟﺮﻗﻢﺍﻟﻬﻴﺪﺭﻭﺟﻴﻨﻲ ‪0.1 ± 7.2‬‬ ‫ﺍﻟﺤﻞﻋﻨﺪ ‪ 25‬ﺩﺭﺟﺔ ﻣﺉﻮﻳﺔ‪:‬‬

‫ﺍﻻﺳﺘﺠﺎﺑﺔﺍﻟﺜﻘﺎﻓﻴﺔ‬
‫™‬ ‫ﺩﻳﻔﻜﻮ™ﻡ ‪Endo Broth MF‬‬
‫ﺗﺤﻀﻴﺮﺍﻟﻮﺳﻴﻂ ﻟﻜﻞ ﺗﻮﺟﻴﻬﺎﺕ ﺍﻟﺘﺴﻤﻴﺔ‪ .‬ﺍﺳﺘﺨﺪﻡ ﺗﻘﻨﻴﺔ ﻣﺮﺷﺢ ﺍﻟﻐﺸﺎء ﻟﺘﻠﻘﻴﺢ‬
‫ﺍﻟﻤﺮﺷﺤﺎﺕ‪.‬ﺍﺣﺘﻀﺎﻥ ﻋﻠﻰ ﻣﻨﺼﺎﺕ ﻣﺸﺒﻌﺔ ﻣﻊ ﻣﺮﻕ ﺇﻧﺪﻭ ‪ MF‬ﻋﻨﺪ ‪ 2 ± 35‬ﺩﺭﺟﺔ‬
‫ﻣﺉﻮﻳﺔﻟﻤﺪﺓ ‪ 2 ± 24‬ﺳﺎﻋﺔ‪.‬‬

‫ﻣﺴﺘﻌﻤﺮﺓ‬ ‫ﺗﻠﻘﻴﺢ‬
‫ﻟﻮﻥ‬ ‫ﺍﺳﺘﻌﺎﺩﺓ‬ ‫‪CFU‬‬ ‫‪™ATCC‬‬ ‫ﺍﻟﺘﻨﻈﻴﻢ‬

‫ﺃﺣﻤﺮﻣﻊ ﺃﺧﻀﺮ‬ ‫ﺟﻴﺪ‬ ‫‪20-80‬‬ ‫‪25922‬‬ ‫ﺍﻹﺷﺮﻳﻜﻴﺔﺍﻟﻘﻮﻟﻮﻧﻴﺔ‬

‫ﻟﻤﻌﺎﻥﻣﻌﺪﻧﻲ‬
‫ﺍﻟﺴﺎﻟﻤﻮﻧﻴﻼﺍﻟﻤﻌﻮﻳﺔ‬
‫ﻋﺪﻳﻢﺍﻟﻠﻮﻥ‬ ‫‪subsp.‬ﺍﻟﻤﻌﻮﻳﺔ‬
‫ﺇﻟﻰﺍﻟﻠﻮﻥ ﺍﻟﻮﺭﺩﻱ‬ ‫ﺟﻴﺪ‬ ‫‪20-80‬‬ ‫‪14028‬‬ ‫ﺍﻟﻨﻤﻂﺍﻟﻤﺼﻠﻲ ﺍﻟﺘﻴﻔﻴﻤﻮﺭﻳﻮﻡ‬

‫ﻣﻠﺤﻮﻅ‬ ‫ﺍﻟﻤﻜﻮﺭﺍﺕﺍﻟﻌﻨﻘﻮﺩﻳﺔ‬
‫‪-‬‬ ‫ﻛﺒﺖ‬ ‫‪3‬‬ ‫‪10×2-310‬‬ ‫‪25923‬‬ ‫ﺍﻟﻤﺬﻫﺒﺔ‬

‫ﺃﻭﻧﻴﻼ‬ ‫‪Esche‬‬
‫ﺍﻟﻤﻮﺭﻳﻮﻡ‬ ‫‪ATCC‬‬
‫‪14028‬‬

‫ﺍﻹﺻﺪﺍﺭﺍﻟﺜﺎﻧﻲ ‪Difco ™ & BBL ™ Manual ،‬‬