LIPIDS REVIEW QUESTIONS

1. Bile acids that are synthesized in the liver are derived from what substance?
a. bilirubin b. fatty acids
c. cholesterol d. triglycerides

2. Which of the following is most associated with nerve tissues?

a. cholesterol b. TAG
c. phospholipids d. sphingolipids

3. Which of the following lipid tests is least affected by the fasting status of the patient?
a. cholesterol b. triglycerides
c. fatty acids d. lipoproteins

4. Lipid storage diseases are inherited disorders due to mutations. What is the cause of
these Diseases?
a. excessive ingestion of fat
b. excessive synthesis of chylomicrons
c. specific enzyme deficiency
d. inability of adipocytes to store fat

5. Cholesteryl esters are formed via esterification of the alcohol cholesterol with what
substance?
a. protein
b. triglyceride
c. fatty acids
d. digitonin

6. Which lipase is removed by allowing the blood to clot so as to prevent interference in

the determination of serum TAG?
a. acid lipase
b. hormone-sensitive lipase
c. hepatic lipase
d. lipoprotein lipase

7. Exogenous TAG are transported in the plasma in what form?

a. phospholipids
b. cholesteryl esters
c. chylomicrons
d. free fatty acids

8. When lipoprotein agarose gel electrophoresis is performed at pH 8.6, which fraction

migrates the fastest to the anode?
a. chylomicrons
b. LDL
c. VLDL
d. HDL

9. The quantitation of HDL level is thought to be significant in the risk assessment of what
disease?
a. pancreatitis
b. cirrhosis
c. coronary heart disease
d. hyperlipidemia

10. The beta-lipoprotein consists primarily of what lipid?

a. fatty acids
b. cholesterol
c. triglycerides
d. phospholipids

11. In the chemical methods of TAG measurement, the first step is the
a. production of glycerol
b. hydrolysis of TAG into FFA and glycerol
c. hydrolysis of lipoproteins
d. hydrolysis of TAG with lipase

12. The reaction needed before proceeding to the Hantzsch reaction is the oxidative
conversion by periodate of glycerol to
a. hydrogen peroxide
b. formaldehyde
c. ethanol
d. glyceraldehyde
13. In the CHOD-PAP method, the cholesterol oxidase reacts with
a. free cholesterol and cholesterol esters
b. free cholesterol and fatty acids
c. free cholesterol only
d. cholesterol esters only

14. Which of the following tests would most likely be included in a routine lipid profile?
a. TAG, FFA, chylomicrons
b. total cholesterol, TAG, phospholipids
c. HDL-chol, LDL-chol, chylomicrons
d. total cholesterol, TAG, HDL-chol

15. A commonly used precipitating agent used to separate HDL-cholesterol from other
lipoprotein fractions is
a. zinc sulfate
b. trichloroacetic acid
c. heparin-manganese
d. isopropanol

16. Which of the following may be described as a variant form of LDL associated with
increased risk of atherosclerotic cardiovascular disease?
a. Lp(a)
b. HDL
c. Apo A-I
d. Apo A-II

17. A patient’s total cholesterol/HDL-cholesterol ratio is 10.0. What level of risk for
coronary heart disease does this result indicate?
a. no risk
b. half average risk
c. average risk
d. twice average risk

18. Which of the following techniques can be used to quantify apolipoproteins?

a. GC
b. ISE
c. ELISA
d. Refractometry

19. The surfactant / albumin ratio by fluorescence polarization is performed to assess

what physiological state?
a. hyperlipidemia
b. coronary heart disease
c. hemolytic disease of the newborn
d. fetal lung maturity

20. A patient’s total cholesterol is 300 mg/dl, HDL cholesterol is 50 mg/dl and his TAG
is 200 mg/dl. What is his calculated LDL-cholesterol?
a. 200
b. 210
c. 290
d. 350

LIPID REVIEW QUESTIONS II

1) Which type of lipase is responsible for the mobilization of TAG stores from theadipose
tissues?
A. Lipoprotein lipase C. Hormone-sensitive lipase
B. Pancreatic lipase D. Acid lipase

2) Transport mechanisms exist for lipids because they are insoluble in plasma. Which
lipid below is usually transported by albumin?
A. Fatty acid C. Cholesterol E. All of these
B. Triglyceride D. A & C only

3) Cholesterol is utilized in the synthesis of:

1. Bile acids 3. Bile salts
2. Steroids 4. Bilirubin

A. 1 & 3 only C. 1, 2 & 3 only E. 4 only

B. 2 & 4 only D. 1, 2, 3 & 4
4) Broad (floating) B- lipoprotein occurs in Type ______ lipoproteinemia and consists of
______.
A. I, chylomicrons C. III, IDL
B. II, VLDL D. IV, VLDL

5) In the colorimetric and fluorometric triglyceride assays, glycerol is oxidized to:

A. Glyceraldehyde C. Formaldehyde
B. Propionic acid D. Carbon dioxide

6) All of the following are components of the Liebermann-Burchard color development

mixture, except:
A. Acetic acid C. Sulfuric acid
B. Acetic anhydride D. Sulfosalicylic acid

7) The substance that is measured to estimate the serum concentration of triglycerides

by MOST methods is:
A. Phospholipids C. fatty acids
B. Glycerol D. pre-beta lipoprotein
8) Which of the following serum proteins migrates with beta-globulins on cellulose acetate
at pH 8.6?
A. Ceruloplasmin C. Haptoglobin
B. Hemoglobin D. C3 component of complement

9) Bile acids that are synthesized in the liver are derived from what substance?
A. bilirubin C. fatty acids
B. cholesterol D. triglycerides

10) Which of the following is most associated with nerve tissues?

A. cholesterol C. TAG
B. phospholipids D. sphingolipids

11) Which of the following lipid tests is least affected by the fasting status of the
patient?
A. cholesterol C. triglycerides
B. fatty acids D. lipoproteins
12) Lipid storage disease are inherited disorders due to mutations. What is the cause
of these diseases?
A. excessive ingestion of fat
B. excessive synthesis of chylomicrons
C. specific enzyme deficiency
D. inability of adipocytes to store fat

13) Cholesteryl esters are formed via esterification of the alcohol cholesterol with what
substance?
A. protein C. triglyceride
B. fatty acids D. digitonin

14) Which lipase is removed by allowing the blood to clot so as to prevent interference in
the determination of serum TAG?
A. acid lipase C. hormone-sensitive lipase
B. hepatic lipase D. lipoprotein lipase

15) Exogenous TAG are transported in the plasma in what form?

A. phospholipids C. cholesteryl esters
B. chylomicrons D. free fatty acids

16) When lipoprotein agarose gel electrophoresis is performed at pH 8.6, which fraction
migrates the fastest to the anode?
A. chylomicrons C. LDL
B. VLDL D. HDL

17) The quantitation of HDL level is thought to be significant in the risk assessment of
what disease?
A. pancreatitis C. cirrhosis
B. coronary heart disease D. hyperlipidemia

18) The beta-lipoprotein consists primarily of what lipid?

A. fatty acids C. cholesterol
B. triglycerides D. phospholipids

19) In the chemical methods of TAG measurement, the first step is the
A. production of glycerol
B. hydrolysis of TAG into FFA and glycerol
C. hydrolysis of lipoproteins
D. hydrolysis of TAG with lipase

20) The reaction needed before proceeding to the Hantzsch reaction is the oxidative
conversion by periodate of glycerol to
A. hydrogen peroxide C. formaldehyde
B. ethanol D. glyceraldehyde

21) In the CHOD-PAP method, the cholesterol oxidase reacts with

A. free cholesterol and cholesterol esters
B. free cholesterol and fatty acids
C. free cholesterol only
D. cholesterol esters only

22) Which of the following tests would most likely be included in a routine lipid profile?
A. TAG, FFA, chylomicrons
B. total cholesterol, TAG, phospholipids
C. HDL-chol, LDL-chol, chylomicrons
D. total cholesterol, TAG, HDL-chol

23) A commonly used precipitating agent used to separate HDL-cholesterol from ther
lipoprotein fractions is
A. zinc sulfate C. trichloroacetic acid
B. heparin-manganese D. isopropanol

24) Which of the following may be described as a variant form of LDL associated with
increased risk of atherosclerotic cardiovascular disease?
A. Lp(a) C. HDL
B. Apo A-I D. Apo A-II

25) A patient’s total cholesterol/HDL-cholesterol ratio is 10.0. What level of risk for
coronary heart disease does this result indicate?

A. no risk C. half average risk

B. average risk D. twice average risk

26) A patient’s total cholesterol is 300 mg/dl, HDL-cholesterol is 60 mg/dl and his TAG is
200 mg/dl. What is his calculated LDL-cholesterol?
A. 200 C. 210
B. 290 D. 350

27) What dye CANNOT be used for staining lipid following electrophoresis?
A. Fat Red 7B C. Sudan black B
B. Ponceau S D. Oil red O

73. The following can be converted into formaldehyde EXCEPT

A. Glycerol C. Methanol
B. TAG D. Glucose

74. Cholesterol is removed from the plasma by:

A. Apoprotein A C. Hepatic conjugated to bile
B. Hepatic LDL receptors D. Lipoprotein lipase

79. In the standing plasma test, what is the lipoprotein that appears as distinct opaque
layer floating at the uppermost surface:
A. HDL C. LDL
B. VLDL D. Chylomicrons
81. If a lipemic serum is ultra-centrifuges and the creamy layer discarded before analysis,
which of these errors will occur:
A. The cholesterol result will be falsely high
B. The triglyceride result will be falsely low
C. Both cholesterol and triglyceride results will be falsely high
D. Only cholesterol will be falsely low

84. Lipids are ordinarily insoluble in aqueous media. In the blood, they are usually
rendered soluble by means of transport proteins. Which transport protein is responsible
for the transport of fatty acids.
A. Lipoproteins C. Globulins
B. Albumin D. A and B only
85. Which lipoprotein will likely predominate in the floating layer of normal fasting plasma
which was ultracentrifuged:
A. Chylomicrons C. LDL
B. VLDL D. HDL

86. After intake of meals, the type of lipoprotein most likely to predominate in the plasma
is the:
A. Chylomicrons C. LDL
B. VLDL D. HDL
88. A deficiency in lipoprotein lipase would likely result in a hyperlipoproteinemia which
will be phenotyped as:
A. Type I C. Type IIb
B. Type IIa D. Type III

89. Which assay below will most likely require a sample blank to reduce sample
interference:
A. Bilirubin determination using a hemolyzed serum
B. Triglyceride determination using the Hantzsch reaction
C. Cholesterol determination using CHOD-PAP
D. Albumin determination by turbidity

90. The process of reverse cholesterol transport is mediated by:

A. HDL C. VLDL
B. LDL D. Chylomicrons

91, The preferred anticoagulant for lipid determination is:

A. Sodium fluoride because it is an enzyme inhibitor
B. Sodium oxalate because it is easily complexes with calcium
C. Sodium EDTA because it does not have an osmotic effect
D. Heparin because it is naturally occurring

92. Which sample below will likely require ultracentrifugation to minimize sample
interference:
A. Icteric serum C. Lipemic serum
B. Hemolyzed sample D. All of the above
93. Which of the following is used to determine VLDL?
A. HDL chol + LDL chol – TAG/5 C. Total Chol – HDL chol x TAG/5
B. TAG/5 D. HDL chol – LDL chol

94. Which of the following procedures is done to prevent interferences in lactescent

serum?
A. storing at 4 degrees C for 60 minutes
B. Dilution with distilled water
C. Warming at 37 degrees C for 30 minutes
D. Ultracentrifugation

95. The human atherosclerotic plaque actually contains

A. Lipid C. Immune complexes
B. Urate D. Tissue debris & platelets