PREGNANCYTEST

I
NTRODUCTI
ON
Therearetwoty
pesoft estforpregnancyt
est
ingoneusesbloodsampl
eandot
her
usesuri
ne.Botht
estdetectthepresencesofbet
asubunitofahormonecal
l
edhuman
chor
ioni
cgonadotr
opi(hCG).

Theuri
nepregnancyt
est(UPT)i
saneasyandrapi
dchromatographi
cimmunoassay
f
orthequant
it
ativ
edetect
ionofhCGinur
inef
ortheearl
ydetect
ionofpregnancy
.

Humanchori
oni
cgonadot
ropi
nisaglycopr
otei
nhormone.HCGappear
sint
heur
ine
ofpr
egnantwomenwi
thi
n2weeks, af
tert
hefir
stmissedmenst
rual
cycl
e.

Pr
egnancytesti
ngisnotint
endedtodi
agnoseadiseasei
tonl
yconf
ir
msanat
ural
pr
ocessoccur
ringinadul
tfemalecapabl
eofrepr
oducti
on.

AI
M
Toconf
ir
mthepr
egnancybyt
hepr
esenceofhumanchor
ioni
cgonadot
ropi
nint
he
ur
ine.

PRI
NCI
PLE
Mostoftheurinepregnancytestki
tsarebasedonlater
al-
fl
owt
echnology.Mostof
t
hem qual
it
ativel
ydetectthepresenceofhCGi nuri
nespeci
menatthesensit
ivi
tyof25
mlU/mL.t
het estusestwolinestoindi
cateresul
ts.

Thetestl
i
neuti
l
izesacombinat
ionofselect
ivel
ydet
ectel
evat
edlev
elsofhCG.The
cont
rol
linei
scomposedofgoatpoly
clonal
antibodi
esandcol
loi
dal
goldpart
icl
es.

Theassayi
sconductedbyaddi
ngauri
nesampletot
hesamplewel
loft
hetest
devi
ceandobser
vingt
hef or
mati
onofcol
our
ed.Thesampl
emigrat
esvi
acapi
l
lary
act
ional
ongthemembranet or
eactwi
tht
hecolour
edconj e.
ugat

Posit
ivesamplesreactwi
tht
hespeci
fi
cant
ibody-
hCG-col
our
edconj
ugat
etof
orma
col
ouredli
neatthetestli
ner
egi
onofthemembrane.

Absenceoft
hiscol
our
edl
i
nesuggest
sanegat
iver
esul
t.Acol
our
edl
i
neal
way
sappeari
n
t
hecont
rol
li
ner
egi
oni
fthet
eat
shasbeenper
for
medpr
oper
ly.
AGGLUTI
NATI
ONREACTI
ON

MATERI
ALSREQUI
REMENT
 Acommer
cial
testki
tthatcont
ainat
estdev
iceanddr
opper
.

 Ur
inesampl
e

 Ti
mer

 Gl
oves

 Wast
edi
sposi
ngofbi
n

PROCEDURE
 Testki
tsar
est
orabl
eatr
oom t
emper
atur
e.

 Remov
ethedev
icef
rom t
hepouchandusei
tassoonaspossi
ble.
  Putt
het
estdev
iceonacl
eanandl
evel
sur
face.

 Holdt
hedropperv
ert
ical
lyandtr
ansf
er3fulldr
opsofur
inet
othespeci
menwel
l
andst
artt
heti
mer.Avoi
dairbubbl
eformat
ion.

 Wai
tfort
hebandst
oappear
.

 Readt
her
esul
twi
thi
n15mi
nut
es.

RESULT
Testposi
ti
ve-bot
hbandsatt
est
(T)andcont
rol
regi
on(
C)

Testnegat
ive–onl
yont
hebandatt
hecont
rol
regi
on(
C)

GURESHOWS;
FI Posi
ti
veandnegat
iver
esul
tofpr
egnancy

I
NTERPRETATI
ON
I
fthetestr
esultshowsdoubl
ebandsi
tindi
cat
est
heoccur
renceofaggl
uti
nat
ion.I
t
i
sposi
ti
veresul
tforpregnancyr
esul
t.

Thebandswer
eappear
edi
nbot
htestandaswel
lascont
rol
regi
on.

Ift
hetestr
esultshowsonlyasingl
ebandatcontr
olregi
onitmi
ghti
ndi
cat
est
he
negat
iver
esul
t,becauseofabsenceofaggl
uti
nat
ionprocess.