SYNERGISTIC EFFECTS OF GUAVA, (Psidium guajava), CASHEW AND

SCENT LEAVES ON SELECTED PATHOGENS

BY

ADEREMI, ADEWALE KOLADE

FPA/ST/19/2-1074

A PROJECT TO BE SUBMITTED TO THE DEPARTMENT OF SCIENCE

TECHNOLOGY, SCHOOL OF SCIENCE AND COMPUTER STUDIES

THE FEDERAL POLYTECHNIC, ADO EKITI, EKITI STATE NIGERIA.

IN PARTIALLY FULFILMENT OF THE REQUIREMENT FOR THE

AWARD OF NATIONAL DIPLOMA (ND) IN SCIENCE TECHNOLOGY.

FEBRUARY, 2022

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 CERTIFICATION
This is to certify that this project was performed and submitted by ADEREMI ADEWALE

KOLADE with Matriculation Number FPA/ST/19/2-1074 to the Department of Science

Laboratory Technology is accepted as a requirement for the award of national diploma in science

technology of The Federal Polytechnic, Ado Ekiti.

__________________ ___________________

MRS F.T AKINRULI

PROJECT SUPERVISOR Date

__________________ ___________________

MRS F.O OLUYEMI Date

COORDINATOR

_________________________

EXTERNAL SUPERVISOR

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 DEDICATION

This project is dedicated to God Almighty, the Alpha and Omega, Lilly of the valley and our

hope of glory.

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 ACKNOWLEDGEMENTS

My utmost appreciation goes to the Almighty God, the Alpha and Omega, Lilly of the

valley, our hope of glory, my very helper in times of needs and our sufficient one, He who

granted me wisdom, knowledge and understanding to run this course.

My appreciation goes to my supervisor, MRS F.T AKINRULI, who immensely tried all

her best to bring this project to reality despite her tight schedule and responsibilities, I pray that

God will lift you higher by His grace. I can’t but give a special acknowledgement to all the

lecturers in my department for their efforts I pray that thy Lord Almighty will continue to be

with you all.

My special unreserved appreciation goes to my parents Mr. and Mrs. ADEREMI who

trained me both morally, Godly and academically. I pray that the kindness of the lord will never

depart from you and you shall live to reap the fruits of your labor by the special grace GOD. I

love you so much.

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 ABSTRACT

The study investigates the synergistic effects of cashew, sent leaves and Guava leaves (Psidium

guajava, Ocimum gratissimum, Anacardium occidentale) leaves extracts on some selected

pathogens such as E. coli, Staph aureus, Proteus mirabilis, Salmonella typhi, Klebsiella

Pneumonae. The phytochemical screening of the three extract were also investigated. The results

indicated that the leaves contain alkaloid, saponin, glycoside, terpernoid, phytate, oxalate,

phenolics as the active ingredients. The mixture of the three extracts exerted high antibacterial

effect on the test organisms with the exception of Proteus murabilis. Therefore, the mixture of

the three leaves can be used to treat ailment caused by the test organisms.

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 TABLE OF CONTENTS

Tittle page i

Certification ii

Dedication iii

Acknowledgements iv

Abstract v

Table of Contents vi

CHAPTER ONE: INTRODUCTION

1.0 Introduction

1.2 Aim of the Study

1.3 Objectives of the Study

CHAPTER TWO: LITERATURE REVIEW

2.0 Literature review

CHAPTER THREE: DATA PRESENTATION AND ANALYSIS

3.0 Determination of Oil Chemical Properties

3.1 Sample and sample preparation

3.2 Determination of Acid Value

3.3 Determination of Saponification Value

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3.4 Determination of Iodine Value (Wij’s method)

3.5 Determination of Peroxide Value

3.6 Determination of cholesterol

3.7 Vitamin A Protocol

CHAPTER FOUR: RESULT

4.0 RESULT

4.1 RESULT

4.2 DISCUSSION

CHAPTER FIVE: CONCLUSIONS AND RECOMMEDATIONS

5.1 CONCLUSIONS

5.2 RECOMMEDATIONS

REFERENCES

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