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(Download PDF) Advances in Food Authenticity Testing 1St Edition Gerard Downey Online Ebook All Chapter PDF
(Download PDF) Advances in Food Authenticity Testing 1St Edition Gerard Downey Online Ebook All Chapter PDF
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Advances in Food Authenticity Testing
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Advances in Food
Authenticity Testing
Edited by
Gerard Downey
Teagasc Food Research Centre
Dublin, Republic of Ireland
Notices
Knowledge and best practice in this field are constantly changing. As new research and
experience broaden our understanding, changes in research methods, professional practices,
or medical treatment may become necessary.
Practitioners and researchers must always rely on their own experience and knowledge in
evaluating and using any information, methods, compounds, or experiments described
herein. In using such information or methods they should be mindful of their own safety and
the safety of others, including parties for whom they have a professional responsibility.
To the fullest extent of the law, neither the Publisher nor the authors, contributors, or editors,
assume any liability for any injury and/or damage to persons or property as a matter of
products liability, negligence or otherwise, or from any use or operation of any methods,
products, instructions, or ideas contained in the material herein.
Library of Congress Cataloging-in-Publication Data
A catalog record for this book is available from the Library of Congress
British Library Cataloguing-in-Publication Data
A catalogue record for this book is available from the British Library
ISBN: 978-0-08-100220-9
1 Introduction 1
G. Downey
References 3
23 Tradition Meets High Tech for Authenticity Testing of Fruit Juices 625
P. Rinke
23.1 Introduction 626
23.2 Overview of Methods Applied in Standard Control 628
Contents xi
Index 755
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List of Contributors
1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8
(A) (B) (C)
Figure 2.1 Analysis of basmati rice varieties by inter simple sequence repeat-polymerase chain
reaction (ISSR-PCR). Amplification in the PCR reactions was performed with primers
AGAGAGAGAGAGAGAGYT (agarose gel a), CACACACACACACACAG (gel b) and
GAGAGAGAGAGAGAGAT (gel c). After amplification, amplicons were separated on
agarose gels and visualized by staining with ethidium bromide. Lane 1 on the agarose gels
contains the molecular weight marker FastRuler (Life Technologies, Inc., Foster City, CA,
USA; fragment lengths: 1500, 850, 400, 200, and 50 base pairs). Amplicons of the following
basmati varieties were separated on the other lanes of gels (A to C): Pusa Basmati-1 (lane 2),
Taraori (HBC-19) (lane 3), Basmati 2000 (lanes 4 and 7), Basmati 515 (lanes 5 and 8) and
Super Basmati (lane 6).
PCR amplicons are separated by gel electrophoresis and can then be visualized by
staining with intercalating fluorescent dyes like ethidium bromide. Alternatively,
one might use primers that are labeled by, eg, radioactivity or by conjugation with
digoxigenin. Fig. 2.1AeC show results of an ISSR analysis of five different varieties
of basmati rice and three different primers. These results will be discussed later in the
context of basmati authenticity testing.
RAPD analysis is a special type of PCR involving short primers with arbitrary
nucleotide sequences of generally 10 bases, which randomly bind to various regions
in the genome. Amplified fragments are analyzed by agarose gel electrophoresis,
and polymorphisms are identified on the basis of banding patterns resulting after stain-
ing with ethidium bromide.
Due to the rapid development of modern technologies for DNA analysis over the
last two decades, RFLP, RAPD, and ISSR markers have lost their importance for
DNA fingerprinting. They are still useful in certain cases in which there is no or
only a little available information about DNA sequences of the targeted genome, for
example, if genomes of rare or exotic organisms have to be analyzed. In food analysis,
DNA fingerprinting based on ISSR markers has been applied to trace and differentiate
wild coffee harvested in Ethiopian rain forests (Zeltz et al., 2005).
The joys of target practice are often hampered by the delays in the
settlement of hits. It takes time and is annoying to be constantly
advancing to the target to examine it. To do away with this, an
illuminated target was constructed that enables the shooter to locate
every hit without leaving his post. To make the device, a square
wooden box of convenient size is obtained. In one side of this, cut a
round hole as large as the largest ring on the targets used. The side
opposite this is fitted with a piece of sheet iron to stop the bullets.
Paint this iron and the interior white. Inside the box, arrange four
electric lights so their rays will be thrown on the hole, as shown.
Candles may be used, if necessary. The lamps must be out of range
of the bullets, that hit the target, and protected by an iron plate. The
targets, painted on thin paper, are fastened over the front of the hole,
and the lights are on, while shooting. Each shot punctures the paper,
and the light streaming through the hole will show the location of the
hit.—Thomas W. Benson, Philadelphia, Pa.
Kinks in Washing Photographic Negatives and
Prints
Photographic defects, spots, stains, etc., are often due to
inadequate washing. In the common method of washing negatives or
prints under the tap, in a dish, the water is not changed quickly
enough. If the negatives are placed film downward and supported at
the edges, the washing is quicker and more thorough. Films as well
as glass negatives are in the same class in the matter of washing.
Films should not stick together, and the sharp edges must not be
permitted to cause scratches.
Bird House Made of an Old Straw Hat
A bird house of an old straw hat is a practical and easily contrived
affair. Cut a hole in the crown of the hat. Then nail the hat against a
board of proper size. To protect the hat against the rain, put a roof
over it, as shown. A perch is also provided. Such a bird house can
be hung against the trunk of a tree, or nailed against a wall. Leaving
the hat in its natural straw color, and painting the rest a dark brown,
produces a satisfactory effect.—C. L. Meller, Fargo, N. D.
Camp Shelter Affords Protection from
Mosquitoes
Lithe Branches Cut in the Woods are Used for the Framework, Which is
Covered with Mosquito Netting