CHIT XUT DC

LIU T L SEN
Nhm 10:

Trn Th Thanh Tm
Trn Thch Tho
Nguyn Vn Thi
Don B Tn

L Hu Thng

Nguyn Vn Chin
Nguyn Vn Chin
Nguyn Khnh
Hunh B Ti

Nguyn Vn T

Ng Quang Thy

CHIT XUT ALCALOID TON PHN

T L CY SEN
Chun b nguyn liu: L sen phi kh, thi nh, sy
kh 60 o C trong t sy, tn nh thnh bt dc liu
bng thuyn tn ri tin hnh chit xut theo hai qui
trnh khc nhau:
Chit nng: Cn chnh xc khong 70,00 g bt l sen,
lm m bng NH4OH 10%. Chit nng bng un hi
lu si vi ethanol 96o (khong 500 ml). Ct thu hi
cn, ha tan cn trong dung dch HCl 5% (pH = 2).
Loi tp cht trong dch acid bng dung dch ether
du ha 3 ln, mi ln 100 - 150 ml. Kim ha dch
nc bng dung dch amoniac c ti pH = 10 - 11.
Chit 5 - 6 ln bng cloroform, mi ln 100 - 150 ml
ly ht alcaloid (kim tra bng thuc th
Dragendorff).

CHIT XUT ALCALOID TON PHN

T L CY SEN
Chit ngui:
Cn chnh xc khong 70,00 g bt l sen, lm m bng
NH4OH 10%.
Ngm vi ethanol 960 (khong 500 ml) trong 3 ngy. Ct
thu hi cn, ha tan cn trong dung dch HCl 5% (pH =
2). Loi tp cht trong dch acid bng dung dch ether
du ha 3 ln, mi ln 100 - 150 ml.
Kim ha dch nc bng dung dch amoniac c ti
pH=10 - 11. Chit 5 - 6 ln bng cloroform, mi ln 100
- 150 ml ly ht alcaloid (kim tra bng thuc th
Dragendorff). Kt qu nh lng alcaloid ton phn
trong dch chit trnh by Bng 3.7:

BNG 3.7: HM LNG ALCALOID TON PHN

TRONG L SEN THU C BNGHAI PHNG
PHP CHIT:

NHN XT:
- Phng php chit nng cho lng alcaloid trong l sen nhiu hn so vi phng php chit
ngui. V vy chng ti la chn phng php chit nng
trong qui trnh chit xut alcaloid ton phn t l sen.
T kt qu kho st, v nng khi lng dc liu ln 320 g, qui trnh chit xut alcaloid
ton phn t l Sen c xc nh nh sau:
Qui trnh chit xut alcaloid ton phn t l cy Sen:
Cn khong 320 g bt dc liu, lm m bng NH4OH 10%, thm 2,5 lt ethanol 96 0 v chit
nng bng cch un hi lu cch thy trong 2 gi. Gn v lc ly dch lc, ra li b dc liu
bng ethanol. Dch chit ethanol c ct thu hi ethanol di p sut gim 60 0C. Ha tan
cn bng dung dch HCl 5% (pH = 2).

Tin hnh chit (3 - 4 ln) ng lng dch chit acid vi ether du ha loi tp cht. Kim
ha dch chit acid bng dung dch amoniac c ti pH = 10 - 11. Chit 5 - 6 ln ng lng
bng cloroform (n khi dch chit nc cho phn ng m tnh vi thuc th Dragendorff), thu
c dch chit cloroform. Ct di p sut gim thu hi dung mi cloroform, ha tan cn
thu c bng 50ml methanol v chuyn ra cc c m, bay hi ti cn, sy 60oC trong 3
gi, thu c 2,85 g alcaloid ton phn.

QUY TRNH CHIT XUT ALCALOID T L SEN:

CM N CC BN THEO DI
BI BO CO CA NHM!