9/27/2014 Tc dng ca Kacimex i vi ung th trn thc nghim

http://www.radinervn.com.vn/chi-tiet-tin/l-2/t-15 1/3
Ngy 27 thng 9 nm 2014 Vin nghin cu iu tr cc bnh him ngho: Nghin cu thuc h tr cai nghin ma ty (Cedemex),thuc h tr iu tr khi u t tho dc (Kacimex), thuc h tr iu tr HIV (Vegakiss)... Bnh vin Hi Vng Cng ty Qu Lm
VIN NGHIN CU IU TR
CC BNH HIM NGHO
TRUNG TM CAI
NGHIN
NG
DY NNG
CH DN
VO VIN
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HOT NG CA VIN
Kt qu nghin cu c
ch tc dng ca
CEDEMEX trong cai
nghin ma tu ti Trung
Quc
Bui lm vic vi on
cn b trng i hc
Griffith - c
on cn b ca B nng
lng Hoa K sang thm
v trang b an ninh cho
bnh vin Hy Vng M
SN PHM CA VIN
Thuc h tr iu tr khi
u t Tho dc Vit Nam
Thuc h tr cai nghin
Ma ty Cedemex
Thuc h tr iu tr tng
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ch s pht trin cc
Virus
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Ngc Oanh
Th cm n ca gia nh
ch B Th Hng Chuyn
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nhn Nguyn Kim L
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13/3/2012 15:40:04
Tc dng ca Kacimex i vi ung th trn thc nghim

TC DNG CA KACIMEX I VI UNG TH GAN THC NGHIM CHUT
NHT TRNG
V NH HNG CA THUC VI H THNG MIN DCH CA CHUT MANG
U

Prof.Huangren Bin.PhD *; Dr Nguyen Phu Kieu PhD ** ;Prof. PhamKim
Man,PhD**
*Vin dc l lm sng -i hc y khoa Naning-China.
** Vin Nghin cu iu tr cc bnh him ngho Vit Nam.

I.VT LIU V PHNG PHP
1. Vt liu
1.1. Cc loi thuc v ha cht th nghim:
- Kacimex, thuc do Cng ty TNHH Qu lm, Vin Nghin cu iu tr cc bnh
him ngho Vit Nam sn xut.
- Cyclophosphamide, thuc do Cng ty hng Thy, Giang t sn xut, s hiu
06041521
- 5-fluorouracil
-Dimethylthiazole, sn phm ca Cng ty Sigma, Hoa k sn xut
- Mi trng RPMI-1640 (Roswell Park Memorial Institute), sn phm ca Cng
ty Hyclone sn xut
- Huyt thanh b ti, sn phm ca Cng ty T Qu Thanh, Hng chu sn
xut
1.2. My mc thit b:
- My c ELISA t ng, Dng c pha dch vi lng, Knh hin vi o ngc
i pha.
- T cy CO
2
- Bung cy siu sch
- T lnh su
1.3. ng vt th nghim
- Chut nht trng, th trng trung bnh 18-22g, c ci mi loi mt na, do
trung tm nui sc vt th nghim, Trng i hc Y Khoa Qung ty cung cp. S
hiu ph chun: Qu SCXK-2003.
- Dng t bo ung th gan chut H
22
, 3 dng t bo khi u ngi 7404,
SGC-7901, HEPG
2
do Vin Dc, Trng i hc Y Khoa Qung ty ct gi.
2. Phng php
Dng phng php so mu MTT vi Dimethylthiazole xc nh tc dng c ch
kh nng tng sinh t bo ca thuc Kacimex.
Dng peptidase x l 3 dng t bo khi u ngi 7404, SGC-7901, HEPG
2
khi
ang pht trin thi k bi s, sau dng mi trng RPMI-1640 thm 10%
huyt thanh thai b ti iu ch thnh huyn dch t bo c nng 10
7
t bo/ml,
cho vo tm nha nui cy loi 96 l, mi l 190 l, bung cy 5%CO
2
, 37
o
C,
m 95% trong 8 gi. Thit k mu thnh:
- Nhm i chiu: ch cho thm 5- fluorouracil
- Nhm chng: ch cho thm mi trng RPMI-1640
- Nhm th nghim: phn bit cho thm cc nng khc nhau 5- fluorouracil v
thuc Kacimex
Nui cy 48 gi, thm 10l xanh Thiazole (5g/L), tip tc nui cy thm 4 gi na
cng iu kin trn, nhanh chng lt ngc tm nha nui cy loi b dung dch
nui cy trong cc l, mi l cho thm 150l DMSO (Dimethyl sulfoxide), lc nh
bng my lc khong 10 pht trong bng ti nhit phng kt ta hon ton
tan ra, dng my c ELISA t ng bc sng t 570-630nmnddeer o tr s OD
ca thnh phn trong cc l, tnh ton t l c ch.
T l c ch (%) = (1 Tr s OD nhm th nghim/Tr s OD nhm i chiu) x 100
Tnh ton s IC
50
(Inhibitory concentration 50%)
3. Th nghim trn ng vt
3.1. nh hng ca Kacimex ln tc ng gy ung th gan thc nghim (H
22
)
chut nht trng
3.1.1. Thit lp m hnh chut mang u
- Chn 50 con chut nht trng, th trng trung bnh 18-22g, c ci mi loi mt
na,
- Vi k thut v khun, bng phng php di thc trong ung th, chc ht dch
mng bng ca chut ung th gan (H
22
) sinh trng 7 ngy, dng nc mui sinh
l pha thnh huyn dch t bo t nng 3x10
7
t bo/ml.
- Dng huyn dch t bo ny tim vo di da vng hm nch ca chut th
nghim, mi con tim 0,2ml.
- Ngy tip theo cn th trng chut, git cht chut v m tch khi u, cn trng
lng khi u.
- Tnh ton t l c ch khi u tng trng theo cng thc: T l c ch = (1 trng
lng khi u trung bnh nhm chut th nghim/trng lng khi u trung bnh
nhm chut i chng) x 100
3.1.2. Phn nhm v cho thuc
- Chut sau khi c cy t bo ung th dng H
22
trn, sau 24 gi c chia
ngu nhin thnh 5 nhm: nhm m hnh, nhm dng Cyclophosphamide, nhm
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NGHIN CU CEDEMEX
TI TRUNG QUC
Nghin cu thc nghim
v kh nng chng ti
nghin (tnh l thuc tinh
thn i vi morphine)
ca thuc Cedemex
nh hng ca Cedemex
i vi hm lng cAMP
v cGMP ti cc vng
no khc nhau trn chut
cng cai nghin morphine
HNG DN IU TR
Hng dn iu tr ct
cn cai nghin ma ty
bng thuc Cedemex ti
cng ng.
Hng dn iu tr cai
nghin ma ty tng hp
ti cng ng bng thuc
Cedemex
LIN KT
GII THIU TIN TC THNH TU KHOA HC KHM CHA BNH NHN XT PHN HI TUYN DNG CN B LIN H TRANG CH
Bo An Ninh Th
Bo Dn Tr
i Truyn hnh Vit
Nam
Truyn hnh K thut s
VTC
9/27/2014 Tc dng ca Kacimex i vi ung th trn thc nghim
http://www.radinervn.com.vn/chi-tiet-tin/l-2/t-15 2/3
ngu nhin thnh 5 nhm: nhm m hnh, nhm dng Cyclophosphamide, nhm
dng Kacimex (liu cao, liu trung bnh v liu thp).
- Nhm m hnh: bm vo d dy 0,4ml nc ct hm nng,mi ngy 1 ln, lin tc
trong 10 ngy.
- Nhm dng Cyclophosphamide tim phc mc 0,2ml c liu 20mg/kg th trng
chut, ngy 1 ln lin tc trong 10 ngy.
- Nhm dng Kacimex (liu cao, liu trung bnh v liu thp): bm d dy 0,4ml
thuc Kacimex pha ch thnh dung dch phn bit t 40, 30, 15mg/ml theo
nhm liu khc nhau lin tc trong 10 ngy.
3.1.3. Xc nh t l c ch khi u ca Kacimex trn ng vt th nghim
Sau ln cho thuc cui cng 24 gi, git cht chut v m tch khi u, cn trng
lng khi u.
Tnh ton t l c ch khi u theo cng thc: T l c ch = (Trng lng khi
u trung bnh nhm chut m hnh - Trng lng khi u trung bnh nhm chut
dng thuc)/ Trng lng khi u trung bnh nhm chut m hnh x 100
3.2. nh hng ca thuc Kacimex n ko di thi gian sng st ca chut nht
trng ung th gan dng H
22
3.2.1. Thit lp m hnh chut nht trng ung th gan dng H
22
trn dch phc mc
- Chn 50 con chut nht trng, th trng trung bnh 18-22g, c ci mi loi mt
na,
- Vi k thut v khun, bng phng php di thc trong ung th, chc ht dch
mng bng ca chut ung th gan (H
22
) sinh trng 7 ngy, dng nc mui sinh
l pha thnh huyn dch t bo t nng 2x10
7
t bo/ml.
- Dng huyn dch t bo ny tim vo khoang phc mc ca chut th nghim, mi
con tim 0,2ml.
3.2.2. Phn nhm v cho thuc
- Chut sau khi c cy t bo ung th dng H
22
trn c chia ngu nhin
thnh 5 nhm: nhm m hnh, nhm dng Cyclophosphamide, nhm dng Kacimex
(liu cao, liu trung bnh v liu thp).
- Nhm m hnh: bm vo d dy 0,4ml nc ct ,mi ngy 1 ln, lin tc trong 10
ngy.
- Nhm dng Cyclophosphamide tim di da 0,2ml c liu 20mg/kg th trng
chut, ngy 1 ln lin tc trong 10 ngy.
- Nhm dng Kacimex (liu cao, liu trung bnh v liu thp): bm d dy 0,4ml
thuc Kacimex pha ch thnh dung dch phn bit t 40, 30, 15mg/ml theo
nhm liu khc nhau lin tc trong 10 ngy.
3.2.3. Quan st nh hng ca Kacimex n thi gian sng st ca chut th
nghim
Sau khi lin tc cho thuc 10 ngy, ngng dng thuc v theo di thi gian sng st
ca chut th nghim. Tnh ton t l ko di thi gian sng st ca chut th nghim
theo cng thc sau:
T l ko di thi gian sng (%) = (S ngy sng st trung bnh ca cc nhm cho
thuc/s ngy sng st trung bnh ca nhm i chng 1) x 100%
KT QU
1. Kt qu th nghim in vivo
Bng 1. Tc dng c ch kh nng tng sinh t bo ca thuc Kacimex l rt r v
hn nhm i chiu.

Cc nhm th nghim Tr s OD T l c ch (%)
Nhm chng 0,573
Nhm i chiu (5-Fu) 0,139 75.767
Kacimex (5mg/ml) 0,124 78,387
Kacimex (1mg/ml) 0,237 58,583
Kacimex (200g/ml) 0,447 21,981
Kacimex (40g/ml) 0,497 13,242

Bng 2. Tc dng c ch kh nng tng sinh t bo ca thuc Kacimex i vi
ngi ung th gan: Kacimex tc dng i vi t bo HEPG2 ngi ung th gan
trong vng 48h , c ch s pht trin i vi t bo HFPG2 khng c r rt.

Cc nhm th nghim tr s OD t l c ch(%)
Nhm chng 0.675
Nhm i chiu(5-Fu) 0.232 65.669
Kacimex(5mg/ml) 0.124 28.194
Kacimex(1mg/ml) 0.484 0.712
Kacimex(2ug/ml) 0.670 1.690

Bng 3. Tc dng c ch kh nng tng sinh t bo ca t bo SGC - 7901 i vi
ngi ung th d dy.
Kacimex tc dng i vi t bo SGC 7901 ngi ung th d dy trong vng
48h , c kh nng c ch t bo kh r rt , ng thi th hin quan h ph thuc
vo liu lng.
Cc nhm th nghim tr s OD t l c ch(%)
Nhm chng 0.728
Nhm i chiu(5-Fu) 0.196 73.015
Kacimex(5mg/ml) 0.291 60.091
Kacimex(1mg/ml) 0.430 40.918
Kacimex(2ug/ml) 0.670 7.942
2. Kt qu th nghim trong c th
Bng 1. nh hng ca Kacimex trong thi k sng i vi chut ung th bng
trng nc
Qua cc nng ca nhm Kacimex i vi thi k ko di s sng ca chut
khi u , c tc dng khng r rt.
Bng 2. nh hng ca Kacimex i vi t l c ch khi u chut
Cc nhm Kacimex thng qua cha tr chut khi u ,cn nng u tng ln vi
9/27/2014 Tc dng ca Kacimex i vi ung th trn thc nghim
http://www.radinervn.com.vn/chi-tiet-tin/l-2/t-15 3/3
Cc nhm Kacimex thng qua cha tr chut khi u ,cn nng u tng ln vi
mc khc nhau , nhng nhm iu tr Kacimex vi liu lng thp lm cho
lng tng cn nng cao hn nhm Cyclophosphamide r rt .
KT LUN:
+ T kt qu nghin cu trn thc nghim, cho thy :
1.Kacimex ca Vin Nghin cu iu tr cc bnh him ngho Vit Nam sn xut t
mt s Tho dc c tc dng phng v iu tr ung th mc t bo.
2.So vi nhm chng Kacimex c tc dng c ch kh nng tng sinh t bo
trn m hnh thc nghim. Nh vy Kacimex c tc dng tt trong iu tr ung th
gan, ung th d dy v kh nng ko di tui th cho ngui mc cc bnh him
ngho ni trn.
3. im khc bit ca Kacimex so vi nhm chng l ngoi tc dng iu tr
khi u khng c s khc bit thuc Kacimex khng c, khng gy tc dng ph
khng c li, thuc cn gip c th phuc hi sc kho.


Cc bi vit lin quan
Th cm n ca ch Nguyn Th Li iu tr khi u nang bung trng bng thuc Kacimex
Kt qu nghin cu c ch tc dng ca CEDEMEX trong cai nghin ma tu ti Trung Quc
Thuc h tr iu tr khi u t Tho dc Vit Nam
Th cm n ca ngi nh bnh nhn
Th cm n ca Bnh nhn b ung th gan iu tr thnh cng bng thuc Kacimex
Th cm n ca ch B Th Hng Chuyn .
Th cm n cu bnh nhn mc bnh him ngho c iu tr bng thuc Kacimex
Bnh vin hi vng mi
Cc bi vit lin quan
Hng dn iu tr ct cn cai nghin ma ty bng thuc Cedemex ti cng ng.
y ban v cc vn x hi ca Quc hi lm vic ti Thi Nguyn
Hng dn iu tr cai nghin ma ty tng hp ti cng ng bng thuc Cedemex
Thm hy vng cho ngi nghin ma ty
"nh sng cui ng hm" cho nhng ngi mc nghin mun on tuyt vi "ci
cht trng"
Thng bo c bit v vic cai nghin ma ty bng thuc Cedemex ti gia nh v cng
ng !
Cc bi vit mi
CEDEMEX v vic p ng yu cu iu tr bnh nghin ma ty hin nay
Bo co khoa hc
Phu thut cho mt bnh nhn u no bng dao Gamma
Phu thut u no bng dao Gamma
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