DNA

DNA

DNA

ATP
ATP
aliquots


ATP

T
T DNA
NAD T ATP
ATP
mM riboATP deoxyriboATP
PCR
DNA
ATP

 DNA
DNA
vector insert C


DNA

BamHIBglII
DNA DNA
DNA

DNA

| BioInform | 36 | 5 2010

Fermentas

C C

PEG
crowding agents

PEG DNA ATP

Rapid ligation kits PEG
PEG
blunt PEG DNA
T
DNA RNA
PEG
DNA DNA .E. coli
PEG
E. coli
T
DNA T
Na K mM
PEG

PEG
PEG

PEG

PEG
E. coli
DNA






ng pUC

DNA

DNA
DNA
l
DNA ladder DNA sample buffer

DNA DNA Clean

and Concentrator Kit Zymo Research
DNA
elution DNA l

DNA
DNA DNA



EDTA Mg
chaotropic
(salt DNA
DNA NanoDrop Spectrophotometer

DNA
Hindlll

BstEll

...








NEB TaKaRa


DNA







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,

yevgenybe@gmail.com :

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.1

.2
.3
.4 BstEII DNA

BioInform | 5 | 37 | 2010