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ANATOMY PRACTICALS 2

REVIEWER
(Based on the Anatomy Manual of DLS-HSI)
Lymphatics, Bone and Cartilage, Muscles, Blood and
Hemopoiesis
By Patricia Gato

Photos from: Patricia Gato, Reg Cruz, Sarah Livelo, Apple


Maturgo, Chle Ow Albo, Jeb Pereda, Giovanni Santos, Nereli,
& Nigel Santos
MUSCLE TISSUE Striated Muscle

Peripherally
located
nucleus

Endomysium

Skeletal muscle
fiber

Skeletal muscle Skeletal muscle

*googled

Epimysium

Peripherally Perimysium
located
nucleus Endomysium

Endomysium Individual
muscle fiber
Skeletal
muscle fiber Fascicle

Blood vessel

Skeletal muscle c.s. Skeletal muscle bundle


MUSCLE TISSUE Striated Muscle (electron micrograph)

A-band I-band M-line Z-line H-band Thin band Thick band

*googled

Mitochondria Triad
Terminal cisternae-T tubule-Terminal cisternae
MUSCLE TISSUE Cardiac Muscle
Intercalated
disk

Centrally
located
Branching
nucleus
Cardiac
Fiber
Endomysium
Binucleated
Cardiac muscle
fiber
fiber

Cardiac muscle Cardiac muscle

Centrally
located
nucleus

Endomysium

Cardiac muscle
fiber

Cardiac muscle c.s.


MUSCLE TISSUE Smooth Muscle

nucleus

Connective
Tissue

Smooth muscle
fiber

Smooth muscle Smooth muscle

nucleus

Connective
Tissue

Smooth muscle
fiber

Arrector Pili Muscle


Smooth muscle c.s. (at pointer)
CARTILAGE & BONE Hyaline Cartilage
Superficial
mesenchyme with
cells

Perichondrium with
fibroblasts

Inner chondrogenic
layer

Differentiating
chondroblasts

Chondrocytes

Isogenous cells

Interterritorial matrix
Trachea Trachea
Nuclei of
chondrocytes

Chondrocyte

Lacunae

Territorial Matrix

Interterritorial
matrix

Trachea
CARTILAGE & BONE Elastic Cartilage

Epiglottis Epiglottis
Perichondrium with
fibrocytes

Chondroblasts

Chondrogenic layer

Cartilage matrix

Chondrocytes

Nuclei of
Chondrocytes

Lacunae

Elastic Fibers
CARTILAGE & BONE Fibrocartilage

Collagen Fiber
Bundle

Chondrocytes

Lacunae

Intervertebral Disc Intervertebral Disc


CARTILAGE & BONE Compact Bone
*structures not
visible in available
slides: Medullary
Inner Cavity containing
Circumferential Bone Marrow
Lamellae

Outer
Circumferential
Lamellae
Volkmanns
Osteocytes canal
within lacunae
Interstitial
Periosteum lamellae

Bone l.s. Bone c.s.

Osteocyte within lacunae

Haversian system/Osteon

Concentric lamellae

Haversian canal

Endosteum

Canaliculi

Cement lines

Bone c.s. Bone c.s.


CARTILAGE & BONE Compact Bone

Sharpeys Fibers

Periosteum of Compact Bone


CARTILAGE & BONE Spongy Bone

Bone Lamellae

Bone fragments
or spicules

Bone marrow
spaces

Bone marrow
cells

Osteocytes
inside lacunae

Endosteum

Spongy Bone Spongy Bone


Current slide: bone undergoing
CARTILAGE & BONE Developing Bone endochondrial/intracartilaginous
bone formation

Epiphysis

Resting Cartilage

Proliferation

Maturation / Hypertrophy

Calcification
Osteocytes in
Lacunae
Diaphysis
Hyaline Cartilage

Spicules Ossification

Bone Marrow Tissue

Medullary Spaces

Periosteum

Perichondrium
Phalanx Phalanx (ZONES)
BLOOD Erythrocytes & Platelets

Platelets are not


cells, they are
cell FRAGMENTS

Blood Smears in Blood Smears in


different different
magnifications magnifications
with focus on with focus on
RBC Platelets
BLOOD Lymphocytes & Monocytes

Blood Smears in Blood Smears in


different different
magnifications magnifications
with focus on with focus on
Lymphocytes Monocytes
BLOOD Neutrophils & Basophils

Blood Smears in Blood Smears in


different different
magnifications magnifications
with focus on with focus on
Neutrophils Basophils
BLOOD - Eosinophils

Blood Smears in
different
magnifications
with focus on
Eosinophils
HEMOPOIESIS Erythopoietic Series

Basophilic Polychromatophilic
Proerythroblast Erythroblast Erythroblast

Normoblast Reticulocyte RBC


HEMOPOIESIS Erythropoietic Series
HEMOPOIESIS Neutrophilic Series

Myeloblast Promyelocyte Myelocyte

Metamyelocyte Stab Cell Segmenter (Mature


Neutrophil
HEMOPOIESIS Eosinophilic & Basophilic Series

Eosinophilic Myelocyte Eosinophilic Metamyelocyte

Metamyelocyte
Basophilic Myelocyte Basophilic Metamyelocyte
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
HEMOPOIESIS Bone Marrow Smear
LYMPHOID - Trachea

Respiratory Epithelium

Collagen Bundles

Lymphocytes

The tracheas lamina propria is


Trachea an example of a loose
lymphoid tissue since
Trachea
lymphocytes present are few
Trachea and far from each other

Respiratory Epithelium

Lymphocytes

Fibroblasts

Macrophage

Trachea
LYMPHOID Upper Esophagus
Upper Esophagus

Non-keratinized Stratified
Squamous Ep.

Lymphocytes

Lamina Propria

The Upper Esophagus lamina


Upper Esophagus propria is an example of a
loose lymphoid tissue since
lymphocytes present are few
Upper Esophagus and far from each other

Non-keratinized Stratified
Squamous Ep.

Lymphocytes

Lamina Propria

Upper Esophagus
LYMPHOID Small Intestine

Lamina propria

Lymphoid Nodule

Lymphocytes

Submucosa

The Jejunums lamina propria


Jejunum is an example of a dense
lymphoid tissue since
Jejunum
lymphocytes present are close
to each other

Lamina propria

Lymphocytes

Submucosa

Jejunum Jejunum
LYMPHOID Small Intestine

Lymphocytes

Payers Patches

The Ileums lamina propria is


Ileum an example of a dense
lymphoid tissue since
Ileum
lymphocytes present are close
to each other

Lymphocytes

Payers Patches

Ileum Jejunum
Ileum
LYMPHOID Veriform Appendix

Lymphocytes

GALT (gut-associated-
lymphoid-tissue)

Appendix The Ileum is an example of a


dense lymphoid tissue since Appendix
lymphocytes present are close
to each other

Lymphocytes

GALT (gut-associated-
lymphoid-tissue)

Appendix Appendix
LYMPHOID Lymph Gland [Focus on Cortex]
Dense Irregular
CT Capsule

Trabeculae

Cortical Sinus

Lymph Nodules
in Outer Cortex

Dense
Lymphoid Tissue
in Inner Cortex

Lymph Gland Lymph Gland

Subcapsular
Capsule Sinus

Cortex

Lymph Gland
LYMPHOID Lymph Gland [Focus on Medulla]

Medullary
Cords

Medullary
Sinuses

Lymph Gland
LYMPHOID - Thymus

Capsule

Trabeculae

Cortex

Medulla

Thymus Thymus
Thymus

Thymocytes
[Parenchymal Cels]

Epitheloid Stromal
Cells [Epithelial
Reticular Cells]

Hassals Corpuscles

Epitheloid Cells

Thymus
LYMPHOID - Spleen

Capsule

Trabeculae

Red Pulp
Spleen
White Pulp

Central Artery

Trabecular Artery

Spleen The Spleen is an example of a dense Spleen


lymphoid tissue since lymphocytes
present are close to each other
Parietal
Lymphoid
Sheaths [PALS]
Cords of Billroth surrounding
white pulp
Splenic Sinusoids cannot be
pinpointed
here*

Follicular Arteries
*Not seen in Red
pulp (LM) in focus:
Penicillar Arteries,
Ellipsoids and
Sheath of
Red Pulp of Spleen Schweigger Seidel White Pulp of Spleen
LYMPHOID - Spleen

*googled *googled

Penicillar (Arteries of the Red


Pulp)

Sheathed Areteries (Ellipsoids)

Sheath of Schweigger Seidel

Red Pulp of Spleen Red Pulp of Spleen


LYMPHOID Palatine Tonsil

Stratified Squamous Non-


keratinized Epithelium

Tonsilar Crypts

Palatine Tonsil The Palatine Tonsil is an example of a Palatine Tonsil


dense lymphoid tissue since
lymphocytes present are close to
each other

Lymphoid Nodules

Germinal Centers

Palatine Tonsil Palatine Tonsil


LYMPHOID Lingual Tonsil

Stratified Squamous Non-


keratinized Epithelium

Tonsilar Crypts

Lymphoid Nodules

Germinal Centers

Lingual Tonsil The Palatine Tonsil is an example of a Lingual Tonsil


dense lymphoid tissue since
lymphocytes present are close to
each other

Stratified Squamous Non-


keratinized Epithelium

Tonsilar Crypts

Lingual Tonsil Lingual Tonsil

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