‫ﻟﻲ‬

‫ﺁ‬‫ﻴﻞ‬
‫ﻠ‬‫‪-‬ﺗﺤ‬
‫‪Sampl‬‬
‫‪ePr‬‬
‫‪epar‬‬
‫‪at‬‬‫‪i‬‬
‫‪onf‬‬
‫‪orHPLCAnal‬‬
‫‪y‬‬‫‪si‬‬
‫ﻟﻰ‪s‬‬
‫ﺔﺇ‬‫ﻴ‬
‫ﻧ‬‫ﺎ‬
‫ﺜ‬‫ﻟ‬
‫ﺍ‬‫ﺓ‬‫ﺮ‬
‫ﺎﺿ‬‫ﻤﺤ‬
‫ﻟ‬‫ﺍ‬
‫ﻴ ﺺ‪-‬‬
‫ﻠﺨ‬‫ﺗ‬
‫ﺮﻱ‬‫ﻧﻈ‬
‫)‪(MCQ‬‬
‫ﺔ‬‫ﺌ‬‫ﻴ‬
‫ﻠﻰﻫ‬
‫ﻋ‬
 1.Whatdoest
hedi
str
ibut
ionconst
ant(
K)r
epr
esenti
nchr ‫ﻱ‬
omat
og‫ﺰ‬
‫ﻳ‬
r‫ﺰ‬
a‫ﻌ‬
p‫ﻟ‬
‫ﺍ‬
h‫ﻰ‬
y?‫ﻨ‬
‫ﺜ‬‫ﻠﻲﻣ‬
‫ﻧﺲﻋ‬
‫ﺃ‬/‫ﺩ‬
‫ﺍ‬‫ﺪ‬‫ﺍﻋ‬

A.Ther
ati
oofanal
yteconcent
rat
ioni
nthest
ati
onar
yphaset
othemobi
l
ephase

B.Thesepar
ati
onef
fi
ciencyoft
hechr
omat
ogr
aphi
ccol
umn

C.Ther
ateofanal
ytemi
grat
ioni
nthechr
omat
ogr
aphi
ccol
umn

D.Thedegr
eeofi
nter
act
ionbet
weent
heanal
yteandt
hest
ati
onar
yphase

I
ntheequat
ionK=Cs/Cm,
whatdoesCsr
epr
esent
?

A.Themol
arconcent
rat
ionoft
heanal
ytei
nthest
ati
onar
yphase

B.Themol
arconcent
rat
ionoft
heanal
ytei
nthemobi
l
ephase

C.Ther
ateofanal
ytemi
grat
ioni
nthechr
omat
ogr
aphi
ccol
umn

D.Theequi
l
ibr
ium const
antf
ort
hechr
omat
ogr
aphi
cpr
ocess

2.Howdoesasolut
ewi
thal
argedi
str
ibut
ionconst
ant(
K)behav
ein
chr
omatogr
aphy?

A.I
tisr
etai
nedmor
est
rongl
ybyt
hest
ati
onar
yphase

B.I
tel
utesf
ast
erf
rom t
hechr
omat
ogr
aphi
ccol
umn

C.I
thasaweakeri
nter
act
ionwi
tht
hest
ati
onar
yphase

D.I
texhi
bit
slowersepar
ati
onef
fi
ciency

3.Whathappenst
othebandofacompoundasi
ttr
avel
sdownachr
omat
ogr
aphi
c
col
umn?

A)I
tnar
rowsandi
ncr
easesi
nampl
i
tude.

B)I
tbr
oadensanddecr
easesi
nampl
i
tude.

C)I
tremai
nsunchangedi
nwi
dthandampl
i
tude.

D)I
tspl
i
tsi
ntomul
ti
plebands.

.
 4.Whi
chcompoundwoul
dbeel
utedmor
erapi
dlyf
rom achr
omat
ogr
aphi
ccol
umn?

A)Onewi
thst
rongaf
fi
nit
yfort
hest
ati
onar
yphase.

B)Onewi
thweakaf
fi
nit
yfort
hest
ati
onar
yphase.

C)Onewi
thahi
ghmol
ecul
arwei
ght
.

D)Onewi
thal
owmol
ecul
arwei
ght
.

5.Whateff
ectdoesi
ncr
easi
ngt
hecol
umnl
engt
hhav
eonchr
omat
ogr
aphi
c
separ
ati
on?

A)I
tdecr
easesr
esol
uti
on.

B)I
tincr
easesr
esol
uti
on.

C)I
thasnoef
fectonr
esol
uti
on.

D)I
tdecr
easest
heel
uti
ont
imef
oral
lcompounds.

6.I
nadsor
pti
onchr
omat
ogr
aphy
,whi
chphasei
sthest
ati
onar
yphase?

A)Gas

B)Li
qui
d

C)Sol
i
d

D)Noneoft
heabov
e

7.Whichtypeofchr
omat
ogr
aphyusesasol
i
dst
ati
onar
yphaseandal
iqui
dmobi
l
e
phase?

A)Li
qui
d-l
i
qui
dchr
omat
ogr
aphy

B)Gas-
li
qui
dchr
omat
ogr
aphy

C)Li
qui
d-sol
i
dchr
omat
ogr
aphy

D)Gas-
sol
i
dchr
omat
ogr
aphy
 8.I
nThi
nLay
erChr
omat
ogr
aphy(
TLC)
,whati
sthest
ati
onar
yphase?

A)Si
l
icagel

B)Wat
er

C)Et
hanol

D)Ni
tr
ogen

9.I
npar
ti
ti
onchr
omat
ogr
aphy
,whati
sthepr
imar
yrol
eoft
hesol
i
dsuppor
t?

A)Separ
ati
ngt
hecomponent
soft
hesampl
e

B)For
mingat
hinf
il
m ont
hest
ati
onar
yphase

C)Cr
eat
inganet
wor
kofmol
ecul
esi
nthemobi
l
ephase

D)Pr
ovi
dingasur
facef
ort
hemobi
l
ephaset
omov
eon

10.
Whi
choft
hef
oll
owi
ngdescr
ibest
hemobi
lephasei
ngas-
li
qui
dchr
omat
ogr
aphy
?

A)Al
i
qui
dst
ati
onar
yphaseonasol
i
dsuppor
t

B)Agasst
ati
onar
yphaseonasol
i
dsuppor
t

C)Al
i
qui
dmobi
l
ephase

D)Agasmobi
l
ephase

11.
Inpar
ti
ti
onchr
omat ogr
aphy,howdoest
hesol
utedi
str
ibut
ebet
weent
hemobi
le
phaseandt
hestat
ionaryphase?

A)Sol
uter
emai
nsex
clusi
vel
yint
hest
ati
onar
yphase.

B)Sol
uteonl
yex
ist
sint
hemobi
l
ephase.
C)Sol
uteequi
l
ibr
atesbet
weent
hemobi
l
eandst
ati
onar
yphases.

D)Sol
utei
sev
enl
ydi
str
ibut
edacr
osst
heent
ir
ecol
umn.

12.
Whi
chi
spol
ari
nNor
mal
phasechr
omat
ogr
aphy
?

A)
stat
ionar
yphase

B)
mobi
l
ephase

C)
A&B

D)
Nonabov
e

13.
Whi
chi
spol
ari
nRev
ersedphasechr
omat
ogr
aphy
?

A)
stat
ionar
yphase

B)
mobi
l
ephase

C)
A&B

D)
Nonabov
e

14.Et
heri
s….
..Mopi
lephase.

A)
Pol
ar

B)
Nonpol
ar

15.
Hexanei
s….
.Mopi
lephase.

A)
Pol
ar

B)
Nonpol
ar

16.
Whati
sthepr
imar
ybasi
sforsepar
ati
oni
nionexchangechr
omat
ogr
aphy
?

A)Mol
ecul
arwei
ghtoft
hemol
ecul
es

B)Char
geoft
hemol
ecul
es

C)Hy
drophobi
ci
tyoft
hemol
ecul
es

D)Si
zeoft
hemol
ecul
es
 17.
Ini
onexchangechr
omat
ogr
aphy
,whati
nter
act
swi
thchar
gedmol
ecul
esi
nthe
sampl
e?

A)Opposi
tel
ychar
gedmoi
eti
esi
nthemobi
l
ephase

B)Opposi
tel
ychar
gedmoi
eti
esi
nthest
ati
onar
yphase

C)Neut
ral
moi
eti
esi
nthest
ati
onar
yphase

D)Neut
ral
moi
eti
esi
nthemobi
l
ephase

18.
Whi
chi
onexchangert
ypei
scov
alent
lycoupl
edt
onegat
ivef
unct
ional
groups?

A)Ani
onexchanger

B)Cat
ionexchanger

C)Neut
ral
exchanger

D)Bi
pol
arexchanger

19.
Insi
zeexcl
usi
onchr
omat
ogr
aphy
,howar
emol
ecul
essepar
ated?

A)Basedont
hei
rchar
ge

B)Basedont
hei
rmol
ecul
arwei
ght

C)Basedont
hei
rhy
drophobi
ci
ty

D)Basedont
hei
rshape

20.
Whi
cht
ypeofmol
ecul
esi
ssi
zeexcl
usi
onchr
omat
ogr
aphypr
imar
il
yappl
i
edt
o?

A)Smal
lor
gani
cmol
ecul
es
B)Lar
gemacr
omol
ecul
es

C)I
oni
ccompounds

D)Aqueoussol
uti
ons

21.
Whati
sanot
hercommonnamef
orsi
zeexcl
usi
onchr
omat
ogr
aphy
?

A)I
onexchangechr
omat
ogr
aphy

B)Af
fi
nit
ychr
omat
ogr
aphy

C)Mol
ecul
arsi
evechr
omat
ogr
aphy

D)Thi
nLay
erChr
omat
ogr
aphy(
TLC)

22.
Whichappl
icat
ionofchr
omat
ographyi
nvol
vesdeter
mini
ngt
heident
it
yofa
mixtur
ewit
hunknowncomponentsbasedonknowncomponent
s?

A)Anal
yze

B)I
dent
if
y

C)Pur
if
y

D)Quant
if
y

23.
Ifaresearcherwantstoseparat
eamixtur
etoisol
ateaspeci
fi
ccomponentf
or
fur
therstudy,whichappl
icat
ionofchr
omatogr
aphywouldbeused?

A)Anal
yze

B)I
dent
if
y

C)Pur
if
y

D)Quant
if
y

24.
Whatappli
cati
onofchr
omatographyi
nvol
vesexami
ningami
xtur
e,i
ts
components,
andthei
rrel
ati
onstooneanother
?

A)Anal
yze

B)I
dent
if
y
C)Pur
if
y

D)Quant
if
y

25.
Whati
sthepr
imar
ypur
poseofHi
gh-
Per
for
manceLi
qui
dChr
omat
ogr
aphy(
HPLC)
?

A)Anal
yzet
hev
iscosi
tyoft
hemobi
l
ephase

B)Separ
ate,
ident
if
y,orquant
if
ycomponent
sinami
xtur
e

C)Det
ermi
net
heboi
l
ingpoi
ntoft
hemobi
l
ephase

D)Measur
ethedensi
tyoft
hecol
umn

26.
Whati
sthef
unct
ionofadegasseri
ntheHPLCmobi
le-
phaser
eser
voi
r?

A)Remov
edi
ssol
vedgasesf
rom t
hemobi
l
ephase

B)Fi
l
teroutdustandpar
ti
cul
atemat
terf
rom t
hesol
vent

C)Measur
ethef
lowr
ateoft
hemobi
l
ephase

D)Regul
atet
hepr
essur
eoft
hemobi
l
ephase

27.
Whi chty
peofpumpi
nHPLCcandel
i
veraconst
antmobi
l
ephasecomposi
ti
on
(isocr
ati
c)?

A)Const
ant
-pr
essur
epump

B)Const
ant
-f
lowpump

C)Gr
adi
entpump

D)I
sot
ropi
cpump

28.
Whati
sakeyr
equi
rementf
oranHPLCpump?

A)Gener
ati
onofpr
essur
esupt
o3000I
bs/
in²

B)Fl
owr
ater
angi
ngf
rom 10t
o20ml
/mi
n
C)Pul
se-
fr
eeout
put

D)Madeofpl
ast
icmat
eri
als

29.
InHPLC,whi
chdetectori
sconcent
rat
ion-
sensi
ti
veandcommonl
yusedf
or
quant
it
ati
veanal
ysis?

A)UVdet
ect
or

B)Massspect
romet
er

C)Ref
ract
ivei
ndexdet
ect
or

D)Fl
uor
escencedet
ect
or

30.
Whatcaninf
luencebot
hqual
it
ati
veandquant
it
ati
veanal
ysi
sinHPLCi
fnot
moni
toredcaref
ull
y?

A)Fl
owr
ate

B)Col
umnl
engt
h

C)Det
ect
ort
ype

D)Mobi
l
ephasecomposi
ti
on

31.
Whati
sthemai
nfunct
ionoft
hesampl
einj
ect
ionsy
stem i
nHPLC?

A)Measur
ethemobi
l
ephasef
lowr
ate

B)Int
roducesampl
esi
ntot
heHPLCsy
stem pr
eci
sel
ywi
thouti
nter
rupt
ingt
hemobi
l
ephase
fl
ow

C)Anal
yzet
hecomposi
ti
onoft
hemobi
l
ephase

D)Fi
l
terouti
mpur
it
iesf
rom t
hesampl
e

32.
Whati
stheadv
ant
ageofaConst
ant
-Fl
owPumpi
nHPLC?

A)Abi
l
ityt
omoni
torf
lowr
atecar
eful
l
yandconst
ant
ly

B)Abi
l
ityt
odel
i
veraconst
antmobi
l
ephasecomposi
ti
on
C)Resi
stancet
ochangesi
nsol
ventv
iscosi
tyduet
otemper
atur
evar
iat
ions

D)Lowcostandeaseofoper
ati
on

33.
Whati
sacommondi
sadv
ant
ageofaConst
ant
-Pr
essur
ePumpi
nHPLC?

A)Di
ff
icul
tyi
nmai
ntai
ningaconst
antmobi
l
ephasecomposi
ti
on

B)Sensi
ti
vi
tyt
ochangesi
ntemper
atur
e

C)Fr
equentpul
sat
ionsi
ntheout
put

D)Hi
ghcostandmai
ntenance

34.
Whichcomponenti
spr
efer
redf
orquant
it
ati
veanal
ysi
sint
hesampl
einj
ect
ion
syst
em ofHPLC?

A)Reser
voi
r

B)I
nject
orv
alv
e(l
oop)

C)Det
ect
or

D)Pump

35.
Whati
sthet
ypi
cali
nter
naldi
amet
eroft
heHPLCcol
umn?

A)10-
20mm

B)4-
5mm

C)30-
40μm

D)3-
10μm

36.
Whichtypeofpacki
ngmater
ialpr
ovi
desbet
tercol
umnef
fi
ciencyandsampl
e
capaci
tyint
heHPLCcolumn?

A)Pel
l
icul
ar
B)Por
ouspar
ti
cles

C)Spher
ical

D)Non-
por
ous

37.
Whati
sthemai
nfunct
ionofanHPLCdet
ect
or?

A)Packi
ngt
hecol
umnbeads

B)Moni
tor
ingt
hemobi
l
ephase

C)Cont
rol
l
ingt
hef
lowr
ate

D)Fi
l
ter
ingt
hemobi
l
ephase

38.
Whi
cht
ypeofHPLCdet
ect
orr
espondst
ophy
sicalpr
oper
ti
esoft
hemobi
lephase?

A)Bul
kPr
oper
tyDet
ect
ors

B)Sol
utePr
oper
tyDet
ect
ors

C)Uni
ver
sal
Det
ect
ors

D)Sel
ect
iveDet
ect
ors

39.
Whati
sthef
unct
ionoft
heHPLCdat
asy
stem andr
ecor
der
?

A)Packi
ngt
heHPLCcol
umn

B)Moni
tor
ingt
hemobi
l
ephase

C)Anal
yzi
ngt
hecol
umnef
fi
ciency

D)Cont
rol
l
ingt
hemodul
esoft
heHPLCi
nst
rument

40.
Whatdoest
heHPLCdat
asy
stem det
ermi
neusi
ngt
hesi
gnal
from t
hedet
ect
or?

A)Packi
ngmat
eri
aldi
amet
er
B)Ret
ent
iont
ime(
tR)andPeakAr
ea

C)Col
umnl
engt
h

D)Mobi
l
ephasecomposi
ti
on

41.
Whati
sthemai
nfunct
ionoft
heHPLCcol
umn?

A)Moni
tor
ingt
hemobi
l
ephase

B)Separ
ati
ngsampl
ecomponent
s

C)Cont
rol
l
ingt
hef
lowr
ate

D)Fi
l
ter
ingt
hemobi
l
ephase

42.
Whatt
ypeofHPLCpacki
ngmat
eri
alhasat
ypi
caldi
amet
erof30t
o40μm?

A)Pel
l
icul
ar

B)Por
ouspar
ti
cles

C)Spher
ical

D)Non-
por
ous

43.
WhichHPLCdetect
ortyperespondst
oachemi
calpr
oper
tyofsol
utes,
suchasUV
absor
banceorf
luor
escence?

A)Bul
kPr
oper
tyDet
ect
ors

B)Sol
utePr
oper
tyDet
ect
ors

C)Uni
ver
sal
Det
ect
ors

D)Sel
ect
iveDet
ect
ors

44.
Whatisakeyadv
ant
ageofSol
utePr
oper
tyDet
ect
orsov
erBul
kPr
oper
tyDet
ect
ors
inHPLC?
A)Bet
tersensi
ti
vi
tyandwi
derr
ange

B)Uni
ver
sal
i
tyofappl
i
cat
ion

C)I
nsensi
ti
vi
tyt
ochangesi
nfl
owr
ateandt
emper
atur
e

D)Lowercostandmai
ntenance