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I.OBJECTIVES: At the end of the discussion, the students are expected to:
K: Relate DNA replication to its complementary structure.
S: Explain the process of DNA replication.
A: Appreciate the application of appropriate planning
tools and techniques
II. TOPIC : Planning techniques, tools and their applications
Reference : Department of Education, Philippines. Senior High School Self Learning Kit
for Organization and Management.
Prayer
Everybody stand and let us pray.
Jemimah, please lead the prayer. Classmates, let us pray. In the name of the father
and of the son and of the holy spirit. Our Father,
Who art in heaven, Hallowed be Thy Name. Thy
Kingdom come, Thy Will be done, On earth as it
is in Heaven. Give us this day, our daily bread,
And forgive us our trespasses, as we forgive
those who trespass against us. And lead us not
into temptation, but deliver us from evil. Amen.
So, when we say that DNA strands are Anti- Students: Cytosine Ma’am!
parallel. What do you mean by that?
Yes, Jerrylyn? (Jerrylyn raise her hand)
Jerrylyn; this means they run in opposite
That’s right! So, from left to right, one of the direction ma’am.
strands run in the five to three direction.
And the other one goes in the three to five direction Students: Yes, we’re ready!
from left to right.
Students: Yes, we’re ready!
Motivation
But before that, let me ask you a question.
When you hear the word REPLICATION, what
comes into your mind? What do you know about
replication?
Let’s start.
Go,
ahead Christian.
(Christian raise his hand)
Excellent! Christian. Let’s give them a BAGTIK
clap. Christian: I guess its REPLICATE Ma’am.
Okay, what do you think is the idea behind the (Students do the BAGTIK clap)
pictures I flashed earlier?
Yes, Rey-Nel?
(Rey-Nel raise his hand)
That’s what I like! This time, Kindly read the (Students go to their family silently)
objectives.
Students:
Task 1.
1. the teacher will provide a pattern of the
components that you will use as the backbone of
your DNA, which will serve you as guide for
your DNA strand.
Color code representation:
Phosphate = Blue
Deoxyribonucleic sugar = Green
Nitrogenous bases as follows:
Adenine = Yellow
Thymine = Pink
Guanine = Violet
Cytosine = Red
Okay, is the procedure clear? 3. after that, make a complementary strand for
the first strand that you made in step 2.
Good, just to remind you again that Adenine and
Thymine are always paired together, and Cytosine Students: very clear Ma’am!
and Guanine are always paired.
You will be given 10 minutes to do the activity.
And here are the components of the backbone of
your DNA.
AGCTGAT
TCGACTA
Remember that Rule
Start now.
…………………After 9 minutes………………
Benders you only have 1-minute remaining to
polish your work. (The students start the task collaboratively
…………………After 1 minute……………… silent)
Okay, time is up. Stop working now and focus on
me. Again, focus on me!
Students: Copy Ma’am.
Alright, this time let’s have Airbenders group to
present their work. Give them a hand please.
Students: Focus on you Ma’am!
Airbenders: Task 2.
Guide Questions for the Airbenders.
Q1: Compare the two new strands of DNA. Are
Thank you! Next slide, please read it aloud they the same of different? Why?
Firebenders.
Q2: How do the nucleotides in DNA pair?
Firebenders: Task 2.
Guide Questions for the Firebenders.
Q3. How do you compare a DNA molecule to a
zipper?
Thank you. So, I have here a ½ sheet of paper. Q4: How does the structure of a DNA molecule
Write your answer there and choose 2 help account for the great variety of life that
representatives to share your answer. You only exist on earth?
have 7 minutes to answer the question. Is that
enough?
Alright, it’s time now Airbenders. Give them a (Students give a round of applause)
hand please.
Airbenders presenter: Hello everyone. So, our
answer in question 3 is that you can compare a
DNA molecule to a zipper because, just like
how a zipper's teeth interlock to close, the
nucleotides in DNA strands pair up in a specific
sequence to form a double helix structure.
Very good!
Now I will introduce to you the key players of Students: Helicase “the unzipping enzyme”.
DNA Replication so that you can get some
background. First is the?
Now, the one that build nitrogenous base to form Students: DNA Polymase.
new DNA strand is what we called?
Students: It is an enzyme that replicate DNA to
Why do we called it DNA Polymase. build a new strand.
And of course, we need to glue the nitrogenous Students: We call the gluer the enzyme ligase, it
base, what do we call by that? is an enzyme which connects two strands of
DNA together.
What do you think would be the nitrogenous bases (Roselyn raise her hand)
that will appear here?
Go ahead Roselyn. Roselyn:
Very Good! Now after DNA replication we have
two daughter strands.
DAUGHTER STRANDS
Application
Assessment
Prepared by:
Ma. Jessibel C. Minguita
Student-Teacher
Submitted to:
Noted by:
BONIFACIO M. DECOY
JR.
Head Teacher III