S KHOA HC V CNG NGH QUNG NGI

TRUNG TM K THUT TIU CHUN O LNG CHT LNG

H THNG QUN L PHNG TH NGHIM THEO ISO/IEC 17025

HNG DNS DNG V HIU CHUN

MY QUANG PH UV VIS
M HIU
: HD.01.14.TC-QNg
LN BAN HNH : 02

Chc danh

Ngi bin son

Th nghim vin

Ngi sot xt
Qun l cht lng

Ngi ph duyt
Gim c

Trng Hong Diu Linh

22/4/2011

Trn Th Cm Vn
25/4/2011

Trn Ngc Khi

01/5/2013

Ch k
H v Tn
Ngy

N V S DNG :

HNG DN S DNG V HIU CHUN

MY QUANG PH UV VIS

1. Mc ch v phm vi p dng
Vn bn ny hng dn cch thc vn hnh, bo tr, hiu chun, x l s c My
quang ph UV VIS nhm thng nht trong vic s dng v bo qun thit b.
Vn bn ny p dng cho cc th nghim vin s dng thit b ti Trung tm k
thut tiu chun o lng cht lng Qung Ngi.
2. Ti liu vin dn
Quy trnh kim sot ti liu, m hiu: QT.01/TC-QNg
Quy trnh kim sot h s, m hiu: QT.02/TC-QNg
Quy trnh qun l trang thit b, m hiu: QT.13/TC-QNg
Catologue ca Nh sn xut
3. Thut ng, nh ngha, t vit tt
3.1. Thut ng, nh ngha
Khng
3.2. T vit tt
PTN

: Phng th nghim

4. Ni dung
4.1. Vn hnh
4.1.1. Chun b vn hnh
- Ni my vi ngun in 220 V
4. 1.2. Thc hin vn hnh
- M cng tc my.
- Bt my vi tnh iu khin.
- Nhp p vo biu tng UVWINLAP trn Desktop khi mn hnh phn
mm x l s xut hin.
- My s t khi ng trong vng 3 pht.
- vo ng dng phn tch nh lng ta vo Menu Application

- Chn Con khi mn hnh s xut hin hp hi thoi.

Bc 1:
Nhn vo Tab Conc: khai bo cc tham s v kiu o, bc sng, b
chnh nn hay khng, kiu ng chun.
+ Trong MEASUREMENT:
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 Ordinate mode: chn Single wavelengths.

Baseline correction: chn No correction (khng b chnh nn).
Curve/Factor : Dng phng trnh ng chun
Intercept :
Concentration unit: n v nng cn o.
+ Trong WAVELENGTHS:
Anlytical: Chn bc sng mun o.
Number of replicates: S ln lp li.
Number ofto average: S mu ly trung bnh.
Method info: Thng tin ca phng php
Bc 2:
Nhn vo tab Refes: Khai bo cc tham s v ng chun nh s im
chun, nng cc dung dch chun.
Use existing calibration: S dng ng chun c.
Recalibration:
Calibration mode:
Enter calibration edit mode: to ng chun mi
Number of references : S im chun( dng ng chun t nht
phi c 3 im)
Nhp di cc nng chun bit vo mc bn cnh nh hnh di,
nng cc im chun ny bn phi chn sao cho ph hp vi tiu
chun cho php.
Bc 3:
Nhn tab sample: khai bo cc tham s v mu o, s lng mu o, tn
mu, tn file kt qu.
Result Filename : tn kt qu
Calculation factor:
Number of sample: s lng mu o.
Bc 4:
Nhn tab Ouput :
Autoprent : result table( in kt qu).
Xut hin bng Method vo File File name lu vo.
Auto Start: Bt u t ng lu.
-

Sau khi ci c xong cc thng s ta nhn phm Start bt u qu trnh o.

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a cuvet mu trng vo bung o ri nhn OK.

a ln lc cc dung dch chun vo bung o ri nhn OK. Sau khi dng

xong ng chun ta bt u o mu v thu nhn kt qu. Kt qu o c th
xem trc tip trn ca s Result Window hoc in ra t my in.

4.1.3. Kt thc vn hnh

- Ra cuvet, v sinh my.
- Thot phn mm ri tt n UV.
- Ghi nht k s dng thit b.
4.2. Kim tra, bo tr
4.2.1 Chu k kim tra, bo tr
6 thng
4.2.2. Ni dung kim tra, bo tr
- V sinh my.
- Kim tra nng lng n D2 hoc thi gian s dng n nhm d kin c
thi dim phi thay n.
- Kim tra cc o.
- Kim tra phng ng nn.
- My phi hat ng bnh thng v t yu cu v cc o cng nh
phng ng nn.
4.3. Hiu chun
4.3.1. Chu k hiu chun
3 thng
4.3.2. Hng dn hiu chun
4.3.2.1. Chun b hiu chun
a. Cht chun Ha cht Dng c:
- Kali dicromat .
- H2SO4 0,01N
- Bnh nh mc 1000 ml, 100 ml: loi A.
b. iu kin mi trng:
- Nhit : 20 - 25 0C.
- m tng i: di 75 %.
c. Yu cu:
- Xc nh h s hiu chun ca hp th ca my UV VIS bng dung dch
K2Cr2O7 .
d. Chun b dung dch kim tra:

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Cn chnh xc 60,06mg K2Cr2O7 chun khan ri em ha tan trong dung

dch H2SO4 0,01N nh mc n 1000ml

e. Kim tra v nh gi kt qu:

-

S dng cuvet 10mm o hp th ca dung dch chun b trn. Kt

qu thu c phi t yu cu quy nh di y.

Bc sng

235nm

257nm

313nm

350nm

hp th

0.748

0.865

0.292

0.640

Gii hn cho
php

0.740-0.756

0.856-0.874

0.289-0.295

0.634-0.646

4.3.2.3. Kt thc hiu chun

-

Lp Bo co kt qu hiu chun .

Cp nht H s l lch thit b v ni dung, kt qu hiu chun thc hn.

(BM.01/QT.13/TC-QNg).

V sinh thit b, khu vc thc hin

4.4. X l s c
Tu trng hp c th x l (theo catalogue)
5. H s cng vic
H s theo di s dng thit b gm:
TT

Tn gi h s

M hiu

Ni
lp

Ni lu

H s l lch thit b

BM.01/QT.13/TC-QNg

PTN

PTN

Bo co kt qu hiu
chun my quang
ph

BM.01/QT.13/TC-QNg

PTN

PTN

Thi gian lu
Theo thit b

nt

Cc h s ny c c nhn, n v lin quan lp v kim sot theo qui

nh ti Quy trnh kim sot h s, m hiu: QT.02/TC-QNg
6. Ph lc
-------------------------

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