LIN KT T BO

(Cell junction)

Bin son: TS. Phm Ngc Khi

(B mn M Phi - Di truyn, i hc Y khoa Phm Ngc Thch)

Email: pnkhoi@pnt.edu.vn
 The major features of eukaryotic cells

Figure 1-30 Molecular Biology of the Cell, Fifth Edition ( Garland Science 2008)
NI DUNG

1. Tng quan v lin kt t bo

2. Lin kt nghn

3. Lin kt khe

4. Lin kt neo

5. Th lin kt (desmosome)

6. Th bn lin kt (hemidesmosome)
 Figure 1.3 Living systems such as the human body consist of closely interrelated elements.

Molecular Cell Biology, 7th edition

 Three key factors maintain the cellular organization of
tissues

Figure 20-34 Essential Cell Biology ( Garland Science 2010)

Lin kt t bo

- Hnh thc lin kt c th ch l kt gn im hay kt

gn ton b chu vi t bo nhm:

+ chng li s xm nhp qua li khong gian bo gia

cc t bo

+ cho php c s trao i cht gia hai t bo cnh

nhau
Lin kt t bo

- Lin kt t bo:

+ cu trc tn ti bn trong m

+ phc hp protein

s gn kt gia 2 t bo k cnh + gia t bo vi

cht nn ngoi bo
 Two main ways in which animal cells are bound
together

Figure 19-1 Molecular Biology of the Cell ( Garland Science 2008)

Lin kt t bo

- Cu trc: mng t bo

- Chc nng: gn kt cc t bo vi nhau + gn kt t

bo vi cht nn ngoi bo

- Tnh cht:

+ tm thi

+ n nh
 A summary of the various cell junctions found in a
vertebrate epithelial cell, classified according to their
primary functions

Figure 19-3 Molecular Biology of the Cell ( Garland Science 2008)

 The recognition of the cell-surface carbohydrate on
neutrophils is the rst stage of their migration out of
the blood at sites of infection

Figure 11-39 Essential Cell Biology ( Garland Science 2010)

LIN KT NGHN

(Tight junction)
 Occluding junctions (involving claudin proteins) seal
gaps between epithelial cells

Figure 19-2b Molecular Biology of the Cell ( Garland Science 2008)

 A model of a tight junction

Figure 19-26b Molecular Biology of the Cell ( Garland Science 2008)

Lin kt nghn

- Cn gi l vng bt (zonula occludens)

+ zonular: kt dnh kiu di/bng bao quanh ton b chu

vi t bo

+ occluden: kt dnh khng cn khong cch gia cc t

bo

- L hnh thc lin kt c phn cao mt trn (mt

nh) ca cc t bo
Lin kt nghn

- Di knh hin vi in t, mng bo tng ca hai t

bo k nhau c ch dnh cht nh sp nhp vo nhau

- Tiu bn ct ng lnh (cryofracture) cho thy trong lin

kt nghn, mng bo tng c nhng ch li v
khuyt to thnh mt di di

- Mt s ch li v khuyt c lin quan mt thit vi

tnh thm ca t bo
Lin kt nghn

- Biu m ng ln gn thn c t ch li v ch
khuyt cho php nc v cc cht ha tan i qua hng
ro t bo biu m nhiu hn

- Biu m chuyn tip ca bng quang c nhiu ch

li v khuyt v hu nh khng cho dch thm qua
Lin kt nghn

- Cu trc: hng ro ngn cch gia tm biu m v

khong khng ngoi bo

- Chc nng: vnh ai gii hn s khuch tn chn lc

vi nc, cc ion, nhng phn t ha tan c trng
lng phn t ln cng nh s di chuyn ca t bo
Lin kt nghn

- Chc nng chnh ca lin kt nghn:

+ bt kn

+ ngn chn s xm nhp cc cht qua khong gian

bo t trong ra ngoi hay ngc li
 The role of tight juctions in allowing epithelia to serve
as barriers to solute diffusion

Figure 20-23a, b Essential Cell Biology ( Garland Science 2010)

 A model of a tight junction

Figure 20-23c Essential Cell Biology ( Garland Science 2010)

 Adherens junction between epithelial cells in the small
intestine

Figure 19-15 Molecular Biology of the Cell ( Garland Science 2008)

 The role of tight junctions in transcellular transport

Figure 19-23 Molecular Biology of the Cell ( Garland Science 2008)

 The structure of a tight junction between epithelial
cells of the small intestine

Figure 19-25 Molecular Biology of the Cell ( Garland Science 2008)

 A junctional complex between two epithelial cells in the
lining of the gut

Figure 19-27 Molecular Biology of the Cell ( Garland Science 2008)

 Intercellular junctions form the epithelial barrier

http://www.qiagen.com/vn/products/genes%20and%20pathways/pathway%20details?pwid=108&action=Deny
 Vibrio cholerae and tight junction

http://www.qiagen.com/vn/products/genes%20and%20pathways/pathway%20details?pwid=108&action=Deny
 Helicobacter pylori and tight junction

http://en.wikipedia.org/wiki/Helicobacter_pylori
 Helicobacter pylori and tight junction

Wroblewski and Peek, Cell Communication and Signaling ,2011, 9:29

 Helicobacter pylori infection

Netters Illustrated Pharmacology, Robert B. Raffa, Scott M. Rawls, 2014

LIN KT KHE

(Gap junction)
 Channel-forming junction (composed of connexin or
innexin proteins) from passageways for small
molecules and ions to pass from cell to cell

Figure 19-2c Molecular Biology of the Cell ( Garland Science 2008)

 Gap junctions

Figure 19-34a Molecular Biology of the Cell ( Garland Science 2008)

Lin kt khe

- Hnh thc lin kt c th c bt k v tr no mt

bn ca mng bo tng tt c cc loi m, nht l
m c

- Cho php trao i cht qua mng bo tng

- Trong tiu bn siu vi ct lnh c th nhn thy hnh nh

nhng m trn trn b mt bn ca mng bo tng
Lin kt khe

- c cu to bi 6 n v cu trc gi l connexin
xp thnh vng trn quanh mt l gia c ng
knh khong 1.5nm, ton b cu to ny gi l
connexon

- Cc phn t connexin ni lin 2 mng bo tng cnh

nhau to nn mt knh a nc cho php s trao i
cht gia hai t bo

- Cc nghin cu mc phn t cho bit connexin

phn b khc nhau cc knh khc nhau chuyn bit
cho tng chc nng c th
Lin kt khe

- Cho php trao i cht gia cc t bo vi cc phn t

c khi lng < 1500 Da

- Cc phn t ca mt s hormone, AMP, GMP c th

trao i qua hnh thc lin kt khe gip cho ton khi
tng cc ch tit hot ng ng b ch khng ch
ring l tng t bo ch tit

- t bo c tim, hnh thc lin kt khe c vai tr quan

trng trong hnh thnh nhp tim
Lin kt khe

- Cu trc: dng tm c cha knh ni bo ln

- Chc nng: dng ni kt vng bo tng ca hai t

bo cnh nhau
 Gap junctions provide neighboring cells with a direct
channel of communication

Figure 20-29a Essential Cell Biology ( Garland Science 2010)

 Signaling via gap junctions

Figure 15-7 Molecular Biology of the Cell ( Garland Science 2008)

 Determining the size of a gap-junction channel

Figure 19-33 Molecular Biology of the Cell ( Garland Science 2008)

 The organization of connexins into cennexons and
cennexons into intracellular channels

Figure 19-34b Molecular Biology of the Cell ( Garland Science 2008)

 Gap junctions as seen in the electron microscope

Figure 19-35 Molecular Biology of the Cell ( Garland Science 2008)

 Cardiac muscle

Junqueiras basic histology (text & atlas), 13th edition, Anthony L. Mescher, McGraw-Hill, 2013.
 Intercalated disk's two regions

BRS Cell biology and histology, 7th edition, Leslie P. Gartner, James L. Hiatt., Wolters Kluwer Health, 2015.
 Cardiac muscle cells

BRS Cell biology and histology, 7th edition, Leslie P. Gartner, James L. Hiatt., Wolters Kluwer Health, 2015.
CU LIN BO

(Plasmodesmata)
 Figure 20.40 Structure of the plant cell wall.

Molecular Cell Biology, 7th edition

 Figure 20.41 Plasmodesmata.

Molecular Cell Biology, 7th edition

LIN KT DNH

(Anchoring junction)
Lin kt dnh

1. Lin kt neo (adherens junction)

2. Desmosome
3. Hemidesmosome + lin kt ti ch
LIN KT NEO

(Adherens junction)
Lin kt neo

- Cn gi l vng dnh (zonula adherens)

- L hnh thc lin kt c nhiu loi t bo

- Hnh thc lin kt ny bao quanh ton b chu vi mt

t bo v kt dnh t bo ny vi cc t bo k bn
Lin kt neo

- c im cu trc: nhiu siu si t thnh tm m

mu mt trn ca mng bo tng

- Cc siu si ny l mt t hp bao gm cc siu si

actin, siu si trung gian v spectrin
 Anchoring junctions

Table 19-2 Molecular Biology of the Cell ( Garland Science 2008)

Lin kt neo

- Cu trc:

+ -catenin

+ -catenin

+ cadherin xuyn mng

 Cadherin molecules mediate mechanical attachment of
one cell to another

Figure 20-24 Essential Cell Biology ( Garland Science 2010)

 Adherens junctions between epithelial cells in the
small intestine

Figure 19-15 Molecular Biology of the Cell ( Garland Science 2008)

TH LIN KT

(Desmosome)
Th lin kt

- Desmosome:

+ desmos: band (di)

+ soma: body (th)

- Cn c tn gi khc l kt dnh im (macula

adherens)

- L mt cu trc phc hp hnh a c b mt bn

mng bo tng kt gn vi mt cu trc ging y nh
vy mng bo tng i din
Th lin kt

- Mt bn mng bo tng ni c th lin kt thng

phng v c khong gian bo rng >30nm, so vi
khong gian bo thng thng l 20nm

- Bo tng t bo ni c th lin kt to nn mt bn
hnh trn gi l tm bo tng (tm kt dnh,
attachment plaque) vi hn 12 loi protein khc nhau

- Rt nhiu siu si keratin trung gian an xen hay an

xon vo tm kt gn
Th lin kt

- Do cc siu si trung gian rt chc chn nn th lin

kt to nn mt s kt gn cht ch gia cc t bo

- cc t bo khng phi l biu m, cc siu si trung

gian khng l cc siu si keratin m l cc protein nh
desmin, vimentin

- c bit, protein nhm cadherin thng c trong cc

th lin kt

- Trong thc nghim, ngi ta ghi nhn s kt gn ca

th lin kt b ph hy khi loi b ion Ca2+
Th lin kt

- Hnh thc kt dnh t bo (intercellular adhesion) ny

c thc hin ch yu bi cc phn t glycoprotein
nm xuyn mng bo tng gi l cadherin

- Khi thiu ion Ca2+, cadherin s mt i tnh nng kt

dnh
Th lin kt

- Desmosome:

+ mi hn gia cc t bo biu m

+ lin kt chc chn gia nhng t bo m c v biu

m
 The folding of an epithelial sheet to form an epithelial
tube

Figure 19-17a Molecular Biology of the Cell ( Garland Science 2008)

Th lin kt

- Desmosome:

+ tm bo tng

+ cadherin

+ siu si keratin
 Cadherin molecules mediate mechanical attachment of
one cell to another

Figure 20-24 Essential Cell Biology ( Garland Science 2010)

 Homophilic versus heterophilic binding

Figure 19-8 Molecular Biology of the Cell ( Garland Science 2008)

 Cadherin structure and function

Figure 19-9a Molecular Biology of the Cell ( Garland Science 2008)

 Cadherin structure and function

Figure 19-9b Molecular Biology of the Cell ( Garland Science 2008)

 Cadherin structure and function

Figure 19-9c Molecular Biology of the Cell ( Garland Science 2008)

 Cadherin shifts and melanoma cell invasiveness

Figure 14.16a The Biology of Cancer ( Garland Science 2007)

 Cadherin shifts and melanoma cell invasiveness

Figure 14.16b The Biology of Cancer ( Garland Science 2007)

 Desmosomes link the keratin filaments of one cell to
those of another

Figure 20-27 Essential Cell Biology ( Garland Science 2010)

 Desmosomes

Figure 19-17b Molecular Biology of the Cell ( Garland Science 2008)

 Desmosomes

Figure 19-17c, d Molecular Biology of the Cell ( Garland Science 2008)

 Transmembrane adhesion proteins link the
cytoskeleton to extracellular structures

Figure 19-4 Molecular Biology of the Cell ( Garland Science 2008)

 Pemphigus vulgaris and desmosomes

http://vi.wikipedia.org/wiki/Pemphigus
 Pemphigus vulgaris and desmosomes

http://classes.kumc.edu/som/cellbiology/connections/intermediate/tut2.html
 Pemphigus vulgaris and desmosomes

http://www.anatomie.uniwuerzburg.de/institutelehrstuehle/lehrstuhl_fuer_anatomie_ii/forschungsbereiche/pathogenese_der_pemphigus_erkran
kung/english_version_pathogenesis_of_the_pemphigus_disease
TH BN LIN KT

(Hemidesmosome)
Th bn lin kt

- C gia mt s loi t bo biu m v mng y, c

c im cu to l mt na ca th lin kt, c vai tr
gn t bo biu m vo mng y n

- Khc vi th lin kt ch yu c nhiu cadherin, th

bn lin kt ch yu c integrin - protein xuyn mng
bo tng, c cc th th chuyn bit cho cc phn t
ngoi bo nh laminin, collagen IV
Th bn lin kt

- Hemidesmosome:

+ siu si trung gian

+ lamina y
 Desmosomes, hemidesmosomes and the intermediate
filament network

Figure 19-18 Molecular Biology of the Cell ( Garland Science 2008)

 Hemidesmosomes anchor the keratin filament in an
epithelial cell to the basal lamina

Figure 20-28 Essential Cell Biology ( Garland Science 2010)

 Hemidesmosomes

Figure 19-46 Molecular Biology of the Cell ( Garland Science 2008)

 Transmembrane adhesion proteins link the
cytoskeleton to extracellular structures

Figure 19-4 Molecular Biology of the Cell ( Garland Science 2008)

 Anchoring junctions link cell to cell (typically via
transmembrane cadherin proteins) or cell to matrix
(typically via transmembrane integrin proteins)

Figure 19-2a Molecular Biology of the Cell ( Garland Science 2008)

 Organization of the cytoskeleton in polarized epithelial
cells

Figure 16-5 Molecular Biology of the Cell ( Garland Science 2008)

TM TT
 Four functional classes of cell juctions in animal
tissues

Figure 19-2 Molecular Biology of the Cell ( Garland Science 2008)

 Anchoring junctions link cell to cell (typically via
transmembrane cadherin proteins) or cell to matrix
(typically via transmembrane integrin proteins)

Figure 19-2a Molecular Biology of the Cell ( Garland Science 2008)

 Occluding junctions (involving claudin proteins) seal
gaps between epithelial cells

Figure 19-2b Molecular Biology of the Cell ( Garland Science 2008)

 Channel-forming junction (composed of connexin or
innexin proteins) from passageways for small
molecules and ions to pass from cell to cell

Figure 19-2c Molecular Biology of the Cell ( Garland Science 2008)

 Signal-relaying junctions are complex structures,
typically involving anchorage protein alongside
proteins mediating signal transduction

Figure 19-2d Molecular Biology of the Cell ( Garland Science 2008)

Table 19-1 Molecular Biology of the Cell ( Garland Science 2008)
 A summary of the various cell junctions found in a
vertebrate epithelial cell, classified according to their
primary functions

Figure 19-3 Molecular Biology of the Cell ( Garland Science 2008)

 Several types of cell-cell junctions are found in
epithelia in animals

Figure 20-22 Essential Cell Biology ( Garland Science 2010)

Tm tt

- Da vo kiu kt gn cc t bo, cc hnh thc lin kt

c phn thnh 3 nhm chnh:

1. Hnh thc lin kt kt dnh bn hay im: th lin kt,

th bn lin kt

2. Hnh thc lin kt ngn chn s thm nhp qua

khong gian bo gia cc t bo: lin kt nghn,
vng dnh, vng bt

3. Hnh thc lin kt cho thng vi nhau: lin kt khe

 Several types of cell-cell junctions are found in
epithelia in animals

http://greatneck.k12.ny.us/GNPS/SHS/dept/science/krauz/bio_h/images/06_31IntercellJunctions_CL.jpg
 Location and features of junctional specializations
found in epithelial cells

BRS Cell biology and histology, 7th edition, Leslie P. Gartner, James L. Hiatt., Wolters Kluwer Health, 2015.
 Location and features of junctional specializations
found in epithelial cells

BRS Cell biology and histology, 7th edition, Leslie P. Gartner, James L. Hiatt., Wolters Kluwer Health, 2015.
 Location and features of junctional specializations
found in epithelial cells

BRS Cell biology and histology, 7th edition, Leslie P. Gartner, James L. Hiatt., Wolters Kluwer Health, 2015.
 Tight junctions

Wheaters Functional Histology A Text and Colour Atlas, 6th edition, Barbara Young et al, Churchill Livingstone, 2014.
 Gap junctions

Wheaters Functional Histology A Text and Colour Atlas, 6th edition, Barbara Young et al, Churchill Livingstone, 2014.
 Adhesion belt (zonula adherens)

Wheaters Functional Histology A Text and Colour Atlas, 6th edition, Barbara Young et al, Churchill Livingstone, 2014.
 Desmosome (macula adherens)

Wheaters Functional Histology A Text and Colour Atlas, 6th edition, Barbara Young et al, Churchill Livingstone, 2014.
 Hemidesmosome

Wheaters Functional Histology A Text and Colour Atlas, 6th edition, Barbara Young et al, Churchill Livingstone, 2014.
 Figure 20.1 Overview of major cell-cell and cell-matrix adhesive interactions.

Molecular Cell Biology, 7th edition

 Figure 20.10 Principal types of cell junctions connecting the columnar epithelial cells lining the
small intestine.

Molecular Cell Biology, 7th edition

Molecular Cell Biology, 7th edition
 Figure 20.19 Transcellular and paracellular pathways of transepithelial transport.

Molecular Cell Biology, 7th edition

CU HI T LNG GI
CU HI T LNG GI

1. Lin kt nghn c cu to sau, TR MT:

A. Claudin

B. Spectrin

C. ZO1

D. ZO2

E. Cadherin
CU HI T LNG GI

1. Lin kt nghn c cu to sau, TR MT:

A. Claudin

B. Spectrin

C. ZO1

D. ZO2

E. Cadherin
CU HI T LNG GI

2. Bnh pemphigus vulgaris c lin quan n loi lin kt:

A. Lin kt nghn

B. Lin kt khe

C. Desmosome

D. Hemidesmosome

E. Lin kt neo
CU HI T LNG GI

2. Bnh pemphigus vulgaris c lin quan n loi lin kt:

A. Lin kt nghn

B. Lin kt khe

C. Desmosome

D. Hemidesmosome

E. Lin kt neo
CU HI T LNG GI

3. Cc lin kt sau xut hin trong t bo ng vt, TR

MT:

A. Desmosome

B. Hemidesmosome

C. Lin kt neo

D. Lin kt khe

E. Cu lin bo
CU HI T LNG GI

3. Cc lin kt sau xut hin trong t bo ng vt, TR

MT:

A. Desmosome

B. Hemidesmosome

C. Lin kt neo

D. Lin kt khe

E. Cu lin bo
CU HI T LNG GI

4. c t ZO ca Vibrio cholerae gy ra bnh tiu chy do:

A. Ph hy lin kt khe

B. Lm lng lin kt neo

C. Ngn cn lin kt desmosome

D. Ni lng lin kt nghn

E. Cha c cu tr li thch hp
CU HI T LNG GI

4. c t ZO ca Vibrio cholerae gy ra bnh tiu chy do:

A. Ph hy lin kt khe

B. Lm lng lin kt neo

C. Ngn cn lin kt desmosome

D. Ni lng lin kt nghn

E. Cha c cu tr li thch hp
CU HI T LNG GI

5. Desmosome l mt phc hp gm c cc thnh phn

sau y, TR MT:

A. Lamina y

B. Tm bo tng dy

C. Cc siu si keratin

D. Cc protein xuyn mng thuc h cadherin

E. Mng t bo
CU HI T LNG GI

5. Desmosome l mt phc hp gm c cc thnh phn

sau y, TR MT:

A. Lamina y

B. Tm bo tng dy

C. Cc siu si keratin

D. Cc protein xuyn mng thuc h cadherin

E. Mng t bo
CU HI T LNG GI
Ghp cp:
A. Lin kt nghn
B. Lin kt neo
C. Lin kt lin thng (lin kt khe)
D. C 3 loi lin kt k trn
E. Khng c lin kt no k trn

1. Cha nhiu connexon

2. iu khin ng hay m nh hormone, Ca2+, AMP
vng
3. C bn cht l protein
4. C kh nng chu lc c hc cao
CU HI T LNG GI
Ghp cp:
A. Lin kt nghn
B. Lin kt neo
C. Lin kt lin thng (lin kt khe)
D. C 3 loi lin kt k trn
E. Khng c lin kt no k trn

1. Cha nhiu connexon C

2. iu khin ng hay m nh hormone, Ca2+, AMP
vng A
3. C bn cht l protein D
4. C kh nng chu lc c hc cao E (desmosome)
TI LIU THAM KHO

1. Sinh hc, Trnh Vn Bo (ch bin), NXBGDVN, 2012.

2. Sinh hc t bo, Trn Cng Toi, Hunh Duy Tho

(ch bin), NXB HQG TP.HCM, 2015.

3. Essential Cell Biology, 4th edition, Alberts B. et al.,

Garland Science, 2013.

4. Molecular Biology of THE CELL, 6th edition, Alberts B.

et al., Garland Science, 2014.
 XIN CHN THNH CM N
a ch lin lc

TS. Phm Ngc Khi

(B mn M Phi - Di truyn, i hc Y khoa Phm Ngc Thch)

Email: pnkhoi@pnt.edu.vn