Report

595150017-5


Dyspepsia
GERD
Peptic ulcer
IBS





91
15
( ) 15
5 ( ) 4
5 5
0.5 0.5

 2

300 . Flask
Ethanol 300 .
30 1
5
3
Dosage 6 50 mL

+


-
Dose 30 CC TID PC 6 / 50mL
pH 6.5 6.5


 UV 254 UV 365 10%Sulfuric acid
Pla Pip spikespike Pla Pip spikespike Pla Pip spikespike

Planotol Planotol
Piperine Piperine
Gingerol Gingerol
Marker Piperine Piperine
Mobile phase; Toluene : Ethylacetate 95:5
Planotol: Suspect Rf 0.1 TLC/TLC- TLC/TLC-
Piperine : Rf 0.4 /QC densitometer densitometer
Biomarker
Piperine Plaunotol
Alkaloids Driterpenoids
soluble in water (40 mg/L, or Kelnac plaunotol 80mg in corn oil
1g/25L (18C)) 150 mg
Soluble in alcohol (1g/15mL) TLC Mobile phase Chloroform : n-
Propanol 96:4 UV 220
/QC
TLC densitometer
Marker; Piperine STD (Purity unknown; approximate 100%)
Mobile phase; Hexane : Ethyl acetate 3:2

Testing Mobile phase ; Hexane : Ethyl acetate 3:2

Piperine Rf 0.5
 TLC Densitometer Condition
Mobile phase; Hexane : Ethyl acetate 3:2
Piperine Rf 0.5 Spot Volume 10uL
Spot width 7 mm
Spot size 7 mm
Flow rate 1uL/sec
Piperine Rf 0.5 UV detector 340 nm (Kedar R,2007)
Piperine Conc. 2.25mg/mL Serial dilution 6 fold
Sample : 1 spot
; Serial dilution 3 fold
 Result
Piperine Curve
Piperine 1 2 3 4 5
Conc. ug/mL 1125 562.5 281.25 140.625 70.3125
AU 34362.1 25053.2 23455.6 11514.5 4220.8
Piperine STD
25000
y = 90.291x - 1749.8
R = 0.9973
20000

15000
Axis Title

Piperine STD
10000
Linear (Piperine STD)

5000

0
0 50 100 150 200 250 300
Axis Title
Piperine Calculate
Linear equation y = 90.291x - 1749.8

Sample result Dilution1st

Conc. Crude 303mg/ mL 151.7mg/mL
AU 3028.2 20036.9
Piperine *52.91779 241.2943
Equivalence(ug/mL)

* AU


Piperine
piperine

piperine 240 ug/mL 1 dose (6
drop 50 mL) piperine 430 ug/mL
stability Quality control piperine marker
piperine Dyspepsia
Reference
Rout K, Rout O and Mishra S. . Estimation of piperine in commercial Ayurvedic
formulations. Journal of Planar Chromatography - Modern TLC . Volume 20, Issue 6 ,
2007
Prasob-orn Rinthong, Aphacha Jindaprasert, and Wanchai De-Eknamkul. Simple
Densitometric TLC Analysis of Plaunotol for Screening of High-Plaunotol-Containing Plants
of Croton stellatopilosus Ohba. ournal of Planar Chromatography 22 (2009)
, . :
. : , 2554 2