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$ FRPSRVLomR HOHPHQWDU GR PDWHULDO ELROyJLFR & 2 + 1 3 6 QmR IDYRUHFH D HPLVVmR
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VHUSRVVtYHOID]HUVHXPDREVHUYDomRGHTXDOLGDGHVHWRUQDQHFHVViULRUHYHVWLURHVSpFLPHFRP
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& '

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35272&2/26

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SURWRFRORVHQFRQWUDVHGLVSRQtYHOQRFDStWXOR$SrQGLFH

1º Protocolo - Microscopia Ótica

,QWURGXomR j XWLOL]DomR GR 0LFURVFySLR ÏWLFR IRFDJHP H DOLQKDPHQWR GR PLFURVFySLR
SUHSDUDomRGRPDWHULDOELROyJLFR ,OXPLQDomRGH.|KOHU

D 'HVWDFDUDHSLGHUPHGDSiJLQDDED[LDOGDIROKDGHKalanchoe blossfeldiana FDODQFyL
E 0RQWDUHPiJXDFREULUFRPODPHODHREVHUYDU

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0HGLo}HVHP0LFURVFRSLDyWLFD8WLOL]DomRGHRFXODUHREMHWLYDPLFURPpWULFD

3DUHGH FHOXODU
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$ %
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( )

)LJ32EVHUYDomR DR PLFURVFySLR yWLFR GH HVWRPDV GD HSLGHUPH DED[LDO GD IROKD GH Kalanchoe
blossfeldiana $ 8WLOL]DomRGHRFXODUPLFURPpWULFDSDUDPHGLomRGHHVWUXWXUDVHPPLFURVFRSLDyWLFD %
2EVHUYDomRGHXPFULVWDOGHWLPROQRPLFURVFySLRGHFRQWUDVWHLQWHUIHUHQFLDO & HQRPLFURVFySLRFRP
OX]SRODUL]DGD '
2EVHUYDomR GH FROyQLDV GH Nostoc QR PLFURVFySLR GH FRQWUDVWH LQWHUIHUHQFLDO ( H QR PLFURVFySLR GH
IOXRUHVFrQFLD )

2º Protocolo - Microscopia Ótica (Continuação), Observação de Procariotas

2EVHUYDomR GH EDFWpULDV GD VDOLYD Streptococcus Staphylococcus H Bacillus RX GR ,RJXUWH
Streptococcus thermophilusHLactobacillus bulgarus

D &RORFDU QXPD OkPLQD GH YLGUR XPD JRWD GH PDWHULDO D REVHUYDU H HVSDOKiOD QD VXSHUItFLH GH
FP
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J 2EVHUYDU

$OWHUQDWLYDPHQWH SRGH VHU XWLOL]DGD D VROXomR GH 1HZPDQ/DPSHUW VHQGR QHVVH FDVR
QHFHVViULRVHFDUOHQWDPHQWHjFKDPD

2EVHUYDomRGHSUHSDUDo}HVGHILQLWLYDVGHFRFRVEDFLORVHHVSLULORVFRUDGDVFRPVDIUDQLQDRX
FRPDFRORUDomRGH*UDP

8WLOL]DomRGHRFXODUHREMHWLYDPLFURPpWULFD

1~FOHR
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&RFRV
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&pOXOD
HSLWHOLDO
0HPEUDQD
SODVPiWLFD
&LWRSODVPD
&LWRSODVPD
1~FOHR
1~FOHR 0HPEUDQD
SODVPiWLFD
$ %
&RFRV
0HPEUDQD SODVPiWLFD
%DFLORV 1~FOHR
1~FOHR GDFpOXOD HSLWHOLDO 1~FOHR
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/|IOHU $VSHWRV JHUDLV RQGH VH REVHUYDP FpOXODV HXFDULRWDV FpOXODV HSLWHOLDLV OHXFyFLWRV H LQ~PHUDV
FpOXODVSURFDULRWDVVREUHSRVWDVjVFpOXODVHSLWHOLDLV $% 'HWDOKHVGHFpOXODVHSLWHOLDLVFRPQ~FOHREHP
YLVtYHOHDOJXPDVFpOXODVSURFDULRWDV FRFRVEDFLORV VREUHSRVWRV &'(

3º Protocolo - Observação de Procariotas (Continuação), Observação de Algas

Unicelulares, Solitárias, Coloniais e Filamentosas e de Protozoários de Água-Doce

2EVHUYDomRGHFLDQREDFWpULDVFROyQLDVGHNostocRXAnabaenaVSHPDVVRFLDomRFRPRIHWR
DTXiWLFRAzolla filiculoides

D 1R FDVR GD Anabaena GHVWDTXH XPD SHTXHQD SRUomR GD IROKD GH Azolla filiculoides VHFFLRQH
ILQDPHQWH H PRQWH HQWUH OkPLQD H ODPHOD 2EVHUYH HVTXHPDWL]H H OHJHQGH DV FROyQLDV GD
FLDQREDFWpULD

&RP XPD SLSHWD UHWLUH XPD JRWD GD DPRVWUD GH ORGR H FRORTXHD QXPD OkPLQD GH YLGUR
&RORTXHLJXDOPHQWHDOJXQVILODPHQWRVGDVDOJDVTXHOKHVHUmRIRUQHFLGDV0RQWHHQWUHOkPLQD
HODPHODHREVHUYHDRPLFURVFySLR

8WLOL]DomRGHRFXODUHREMHWLYDPLFURPpWULFD

Anab aena VS
+HWHURFLVWR
1yGXOR SRODU
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)LJ32EVHUYDomRDRPLFURVFySLRyWLFRGHFROyQLDVGHFLDQREDFWpULDV $& $VSHWRJHUDOGHFROyQLDVGH

Anabaena VS $ 'HWDOKH GH XPD FROyQLD GH Anabaena VS RQGH p EHP YLVtYHO D SUHVHQoD GH
KHWHURFLVWRVFRPQyGXORVSRODUHV % &ROyQLDVGHGloeocapsaVS &
2EVHUYDomRDRPLFURVFySLRyWLFRGHRUJDQLVPRVHXFDULRWDVXQLFHOXODUHV '= 'HQRWDUDSUHVHQoDGH
RUJDQLWRVQRLQWHULRUGDVVXDVFpOXODVTXHSHUPLWHPLGHQWLILFDUHVWHVRUJDQLVPRVFRPRVHQGRHXFDULRWDV
$OJDVVROLWiULDV Chlamydomonas ' Micrasterias ( Closterium )

* + , -
/ 0 1
2 3 4

5 6 7 8
9 ; =

)LJ3 FRQW 2EVHUYDomRDRPLFURVFySLRyWLFRGHRUJDQLVPRVHXFDULRWDVXQLFHOXODUHV '= 'HQRWDUD

SUHVHQoD GH RUJDQLWRV QR LQWHULRU GDV VXDV FpOXODV TXH SHUPLWHP LGHQWLILFDU HVWHV RUJDQLVPRV FRPR
VHQGR HXFDULRWDV $OJDV VROLWiULDV 'LDWRPiFHDV *- Euglena / $OJDV FRORQLDLV 'LDWRPiFHDV 0
Chlorella 1 Scenedesmus 2 Coelastrum 3 Pediastrum 4 $OJDV ILODPHQWRVDV Spyrogira 5
Cladophora 6 3URWR]RiULRVFLOLDGRVVorticella 7 Stentor 8 Paramecia 9 3URWR]RiULRVUL]ySRGHV
Amoeba ; Pompholyxophrys =

4º Protocolo - Observação da Matriz extracelular (matriz extracelular vegetal ou parede

celular)

&DUDFWHUL]DomRKLVWRTXtPLFDGRVFRQVWLWXLQWHVGHSDUHGHFHOXODU

D (IHWXHFRUWHVWUDQVYHUVDLVHPFDXOHVMRYHQVGHRuscusVS JLOEDUGHLUD
E (IHWXHDFRORUDomRFRPD]XOGHWROXLGLQD

,,PHUVmRHPKLSRFORULWRGHVyGLR PLQ
,,/DYDJHPHPiJXDDFpWLFD PLQ
,,,/DYDJHPHP+2 PLQ
,9,PHUVmRHPD]XOGHWROXLGLQD PLQ
9/DYDJHPHP+2PRQWDJHPHREVHUYDomR

5HVXOWDGRGDFRORUDomR
3DUHGHVFHOXODUHVFHOXOyVLFDV±URVDSDUHGHVFHOXODUHVOHQKLILFDGDVD]XO

$OWHUQDWLYDPHQWHSRGHVHUXWLOL]DGDDGXSODFRORUDomRYHUGHLRGR FDUPLPDOXPLQDGR
,,PHUVmRHPKLSRFORULWRGHVyGLR PLQ
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,,,,PHUVmRHPYHUGHLRGR V
,9/DYDJHPHP+2 PLQ
9,PHUVmRHPFDUPLPDOXPLQDGR PLQ
9,/DYDJHPHP+2PRQWDJHPHREVHUYDomR

5HVXOWDGRGDFRORUDomR
3DUHGHVFHOXODUHVFHOXOyVLFDV±URVDSDUHGHVFHOXODUHVOHQKLILFDGDVYHUGH

,GHQWLILFDomRGHSDUHGHVFHOXOyVLFDVSDUHGHVOHQKLILFDGDVHSDUHGHVVXEHULILFDGDV

D 2EVHUYHDSUHSDUDomRGHILQLWLYDTXHOKHIRLGLVWULEXtGD
E )DoD R HVTXHPD GH XPD VHFomR GR FRUWH H OHJHQGHR DWHQGHQGR j FRQVWLWXLomR GDV SDUHGHV
FHOXODUHV

3DUHGHFHOXODUOHQKLILFDGD 3DUHGHFHOXODUFHOXOyVLFD 3DUHGHFHOXODUOHQKLILFDGD 3DUHGHFHOXODU

HVFOHUrQTXLPD SDUrQTXLPDPHGXODU [LOHPD FHOXOyVLFD IORHPD
$ %
3DUHGHFHOXODUFHOXOyVLFD
SDUrQTXLPDPHGXODU
3DUHGHFHOXODUOHQKLILFDGD
HVFOHUrQTXLPD
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FHOXOyVLFD IORHPD 3DUHGHFHOXODU
OHQKLILFDGD [LOHPD
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HVSHVVDPHQWRVHP8 SRQWXDo}HVGH&DVSDUL
&
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)LJ32EVHUYDomRDRPLFURVFySLRyWLFRGHVHFo}HVWUDQVYHUVDLVGHFDXOHGHRuscusVSDSyVFRORUDomR
FRP D]XO GH WROXLGLQD $& $V SDUHGHV FHOXODUHV FHOXOyVLFDV VXUJHP FRUDGDV GH URVD YLROiFHR DV
SDUHGHVFHOXODUHVOHQKLILFDGDVVXUJHPFRUDGDVGHD]XO
2EVHUYDomR DR PLFURVFySLR yWLFR GH VHFo}HV WUDQVYHUVDLV GH UDL] GH XPD PRQRFRWLOHGyQHD & H GH
XPD GLFRWLOHGyQHD ' DSyV FRORUDomR GXSOD FRP YHUGH LRGR FDUPLP DOXPLQDGR $V SDUHGHV FHOXODUHV
VXEHULILFDGDV FpOXODV GD HQGRGHUPH VXUJHP FRUDGDV GH FDVWDQKR DV SDUHGHV FHOXODUHV FHOXOyVLFDV
FRUDPGHYHUPHOKRHDVSDUHGHVFHOXODUHVOHQKLILFDGDVFRUDPGHYHUGH

5º Protocolo - Observação da Parede Celular (Continuação)

&DUDFWHUL]DomRKLVWRTXtPLFDGDFXWtFXODGDIROKDGHFicusVS iUYRUHGDERUUDFKD HREVHUYDomR
GHFLVWyOLWRV

D (IHWXHFRUWHVWUDQVYHUVDLVQDIROKD
E &RORTXHRVFRUWHVHPYHUPHOKRVXGmR,,,GXUDQWH PLQ
F /DYDJHPUiSLGDHPHWDQROD GHVGLIHUHQFLDomR
G /DYDJHPUiSLGDHPiJXDHPRQWDJHP

2EVHUYDomRGHSRQWXDo}HVGDSDUHGHFHOXODU

D 'HVWDTXH XPD SRUomR GH HSLGHUPH GD SiJLQD GD IROKD RX GR FDXOH GH Dianthus VS FUDYHLUR H
PRQWHHQWUHOkPLQDHODPHOD
E 2EVHUYHDWHQWDPHQWHDRQtYHOGDSDUHGHFHOXODUHGHVHQKHDVSRQWXDo}HVDtH[LVWHQWHV

2EVHUYDomRGHSDUHGHVFHOXODUHVPLQHUDOL]DGDV

D 'HVWDTXHXPDSHTXHQDSRUomRGDHSLGHUPHGRFDXOHGHUrtica dioica XUWLJD 0RQWHHQWUHOkPLQD
HODPHODQXPDJRWDGHiJXD
F )DoDXPHVTXHPDOHJHQGDGR

2EVHUYDomRGHSDUHGHVFHOXODUHVGHUHVHUYD

D )DoDFRUWHVWUDQVYHUVDLVGRIUXWRGHPhoenix dactylifera SDOPHLUD
E (IHWXHDGXSODFRORUDomRFRPiFLGRSHULyGLFR UHDJHQWHGH6FKLII 3$6
,,PHUVmRHPWHWUDKLGUHWRERUHWRGHVyGLR SUHSDUDUQDDOWXUD PLQ
,,ÈFLGRSHULyGLFR PLQ
,,,/DYDJHPUiSLGDHP+2
,9,PHUVmRHPUHDJHQWHGH6FKLII PLQ
F /DYDJHPHPPHWDELVVXOILWRGHVyGLR PLQ
G /DYDJHPHP+2
G 0RQWHHQWUHOkPLQDHODPHODQXPDJRWDGHiJXD2EVHUYHHIDoDXPHVTXHPDOHJHQGDGR

&XWtFXOD
3HG~QFXOR (SLGHUPH FRPSRVWDRX

SOXULHVWUDWLILFDGD
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3RQWXDo}HV
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)LJ33UHVHQoDGHFLVWyOLWRVHPFicus elastica $% 5HSUHVHQWDomRHVTXHPiWLFDGRFLVWyOLWRQRLQWHULRU

GR OLWRFLVWR DR QtYHO GD HSLGHUPH FRPSRVWD GD SiJLQD VXSHULRU GD IROKD GH Ficus elastica $
2EVHUYDomRDRPLFURVFySLRyWLFRGHXPFLVWyOLWRQXPFRUWHWUDQVYHUVDOGDIROKDGHFicusVSVXUJLQGRD
FXWtFXODFRUDGDGHYHUPHOKRGHYLGRjFRORUDomRFRPYHUPHOKRVXGmR,,, %
2EVHUYDomRDRPLFURVFySLRyWLFRGHSRQWXDo}HVGDSDUHGHFHOXODUHPFpOXODVGDHSLGHUPHDED[LDOGD
IROKDGHDianthusVS &'

6º Protocolo - Observação da Matriz extracelular (Matriz extracelular animal)

2EVHUYDomR GH PDWUL] H[WUDFHOXODU HP SUHSDUDo}HV GHILQLWLYDV GH GLYHUVRV WLSRV GH WHFLGRV
DQLPDLV

2EVHUYDomRGHPDWUL]H[WUDFHOXODUHPWHFLGRFDUWLODJtQHR

D (IHWXHFRUWHVQXPDSRUomRGHWHFLGRFDUWLODJtQHRHFRORTXHRVHPiJXD
E &RORTXHRVFRUWHVHPD]XOGHWROXLGLQDGXUDQWHPLQ
F /DYDJHPUiSLGDHPiJXDHPRQWDJHP

)LJ32EVHUYDomR DR PLFURVFySLR yWLFR GH VHFo}HV GH GLYHUVRV WHFLGRV DQLPDLV DSyV FRORUDomR FRP
KHPDWR[LOLQDHHRVLQD6HFo}HVGHWUDTXHLDGHPDPtIHUR $% FRPHSLWpOLRFROXQDUSVHXGRHVWUDWLILFDGR
FLOLDGRHWHFLGRFRQMXQWLYRGLIXVR $

)LJ3 FRQW 2EVHUYDomRDRPLFURVFySLRyWLFRGHVHFo}HVGHGLYHUVRVWHFLGRVDQLPDLVDSyVFRORUDomR

FRPKHPDWR[LOLQDHHRVLQD6HFo}HVGHWUDTXHLDGHPDPtIHUR $% FRPWHFLGRFRQMXQWLYRGLIXVRHWHFLGR
FDUWLODJtQHR % 6HFo}HV GH SHOH KXPDQD & FRP HSLWpOLR SDYLPHQWRVR HVWUDWLILFDGR VHQGR YLVtYHO D
]RQDGHHVFDPDomRHWHFLGRFRQMXQWLYRGLIXVR'HQRWDUDDXVrQFLDGHPDWUL]H[WUDFHOXODUQRVHSLWpOLRVH
DSUHVHQoDGHXPDPDWUL]PXLWRGHVHQYROYLGDQRVWHFLGRVFRQMXQWLYRVGLIXVRHFDUWLODJtQHR

7º Protocolo - Estudo da Plasmólise e Deplasmólise e Determinação da Permeabilidade

Membranar

2EVHUYDomR GD PHPEUDQD SODVPiWLFD SODVPDOHPD RX PHPEUDQD FHOXODU SRU LQGXomR GH
SODVPyOLVH

D 'HVWDTXH XPD SHTXHQD SRUomR GD HSLGHUPH LQWHUQD GH XPD HVFDPD GR EROER GH Allium cepa
FHEROD H PRQWHD HQWUH OkPLQD H ODPHOD HP YHUPHOKR QHXWUR 2EVHUYH H IDoD XP HVTXHPD
OHJHQGDGR
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LUULJDomR2EVHUYHHIDoDXPHVTXHPDOHJHQGDGR
F 6XEVWLWXD D VROXomR VDWXUDGD GH VDFDURVH GR PHLR GH PRQWDJHP SRU +2 2EVHUYH H IDoD XP
HVTXHPDOHJHQGDGR

'HWHUPLQDomRGDSHUPHDELOLGDGHPHPEUDQDU

D )DoD SUHSDUDo}HV GLIHUHQWHV FDGD XPD FRQWHQGR FRUWHV WUDQVYHUVDLV ILQRV GH UDL] GH Beta VS
EHWHUUDED PRQWDGRV HQWUH OkPLQD H ODPHOD QXPD JRWD GH iJXD H REVHUYH QR PHQRU SRGHU GH
DPSOLDomR [ )DoDXPHVTXHPDOHJHQGDGR
E &RQWURODQGR DWUDYpV GD RFXODU GH [ VXEVWLWXD FDGD XP GRV PHLRV GH PRQWDJHP SRU XP GRV
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VROXomRKLSHUWyQLFD1DVFpOXODVSODVPROLVDGDVpSRVVtYHOYLVXDOL]DUDPHPEUDQDSODVPiWLFDDIDVWDGDGD
SDUHGHFHOXODUHRHVSDoRSHULSODVPiWLFR

8º Protocolo - Estudo do Vacúolo

2EVHUYDomRGHFULVWDLVGHR[DODWRGHFiOFLR

D 2EVHUYDomRGHUiILGHV)DoDXPFRUWHORQJLWXGLQDOQDIROKDGHAloeVS DORp RXHPDOWHUQDWLYDQD
HVSDWDGHZantedeschia VS MDUUR 0RQWHHQWUHOkPLQDHODPHODQXPDJRWDGHiJXDHREVHUYH
E 2EVHUYDomR GH GUXVDV 'HVWDTXH D HSLGHUPH GD VpSDOD GH Pelargonium VS VDUGLQKHLUD 0RQWH
HQWUHOkPLQDHODPHODQXPDJRWDGHiJXDHREVHUYH
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H[WHUQDGREROERGHAllium cepa0RQWHHQWUHOkPLQDHODPHODQXPDJRWDGHiJXD RXJOLFHULQD H
REVHUYH

2EVHUYDomRGHFULVWDLVGHLQXOLQD

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E )DoDVHFo}HVILQDVGRIUDJPHQWRGHWXEpUFXORGHDahliaVS
F 0RQWHHQWUHOkPLQDHODPHODQXPDVROXomRGHJOLFHULQDHREVHUYH

2EVHUYDomRGHJUmRVGHDOHXURQD

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E 0RQWHQXPDJRWDGHiJXDHQWUHOkPLQDHODPHODHREVHUYHRVYDF~RORVFRUDGRV
F 'HSRLVGHHIHWXDGRRHVTXHPDVXEVWLWXDRPHLRGHPRQWDJHPSHORPpWRGRGHLUULJDomRSRUXPD
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RFRUUHPDRQtYHOGRYDF~ROR

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SURPRYHDOWHUDo}HVQDFRUPDQLIHVWDGDSHODVDQWRFLDQLQDV +/

9º Protocolo - Observação de Movimentos de Ciclose, Observação de Cloroplastos

2EVHUYDomRGHPRYLPHQWRVGHFLFORVH

D 0RQWH HQWUH OkPLQD H ODPHOD XPD IROKD GH Elodea VS HORGHD H REVHUYH RV
PRYLPHQWRVGHFLFORVH

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D 0RQWH HQWUH OkPLQD H ODPHOD XP ILODPHQWR GH Spirogyra VS HVSLURJLUD H REVHUYH RV
FORURSODVWRV FRP SLUHQRLGHV 6XEVWLWXD SRU LUULJDomR R PHLR GH PRQWDJHP SRU XPD
VROXomRGH/XJROHREVHUYH

2EVHUYDomRGHFORURSODVWRVHPSODQWDVVXSHULRUHV

D )DoD FRUWHV WUDQVYHUVDLV GD IROKD Nerium VS ORHQGUR PRQWH HQWUH OkPLQD H ODPHOD H REVHUYH
6HJXLGDPHQWH SHOR PpWRGR GH LUULJDomR VXEVWLWXD D VROXomR GH PRQWDJHP SRU XPD VROXomR GH
/XJROHREVHUYH

)LJ3D5HSUHVHQWDomRHVTXHPiWLFDGDVHFomRWUDQVYHUVDOGDIROKDGHNerium oleander

Elodea canadensispXPDHVSpFLHH[yWLFDHLQYDVRUDHP3RUWXJDOSHORTXHQmRpSHUPLWLGDDVXDSODQWDomR
HSURSDJDomR

)LJ3E2EVHUYDomRDRPLFURVFySLRyWLFRGHFORURSODVWRVHPElodeaVS $ HPSpyrogiraVS % HHP

Nerium oleander ( $SyV LUULJDomR FRP VROXWR GH /XJRO R DPLGR DFXPXODGR HP UHGRU GRV SLUHQRLGHV
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yWLFR ' GDVHFomRWUDQVYHUVDOGDIROKDGHNerium oleanderSHUPLWHPREWHUXPDYLVmRJHUDOGDHVWUXWXUD
GDIROKD

10º Protocolo - Observação de Plastos

2EVHUYDomRGHOHXFRSODVWRVHFORURSODVWRV

D 'HVWDTXH XPD SRUomR GH HSLGHUPH GH XP FDXOH MRYHP RX GH HSLGHUPH DGD[LDO GD IROKD GH
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VXEVWLWXDSHORPpWRGRGHLUULJDomRDVROXomRGHPRQWDJHPSRUXPDVROXomRGH/XJROHREVHUYH

2EVHUYDomRGHDPLORSODVWRV

D )DoD FRUWHV ILQRV GR WXEpUFXOR GH Solanum tuberosum EDWDWHLUD PRQWH HQWUH OkPLQD H ODPHOD H
REVHUYH 6XEVWLWXD SHOR PpWRGR GH LUULJDomR R PHLR GH PRQWDJHP SRU XPD VROXomR GH /XJRO H
REVHUYH
E )DoDFRUWHVILQRVGDSROSDGRIUXWRGHMusaVS EDQDQHLUD 3URFHGDFRPRHPD

2EVHUYDomRGHFURPRSODVWRV

D 'HVWDTXH XPD SRUomR GD HSLGHUPH GR IUXWR GH Pyracantha VS SLUDFDQWR RX HP DOWHUQDWLYD GD
HSLGHUPH DGD[LDO GD SpWDOD GH Aloe Tropaeolum FDSXFKLQKD RX GH Gerbera JHUEpUD , PRQWH
HQWUHOkPLQDHODPHODHREVHUYH6HJXLGDPHQWHVXEVWLWXDSHORPpWRGRGHLUULJDomRDVROXomRGH
PRQWDJHP SRU XPD VROXomR GH /XJRO H REVHUYH )DoD XPD QRYD SUHSDUDomR H VXEVWLWXD SHOR
PpWRGRGHLUULJDomRDVROXomRGHPRQWDJHPSRUXPDVROXomRGH1D2+HREVHUYH
E )DoD FRUWHV WUDQVYHUVDLV QD UDL] GH Daucus carota FHQRXUD PRQWH HQWUH OkPLQD H ODPHOD H
REVHUYH 6HJXLGDPHQWH VXEVWLWXD SHOR PpWRGR GH LUULJDomR D VROXomR GH PRQWDJHP SRU XPD
VROXomRGH/XJROHREVHUYH

Tradescantia zebrina pXPDHVSpFLHH[yWLFDHLQYDVRUDQD5HJLmR$XWyQRPDGD0DGHLUDHRXWUDVHVSpFLHV
GH Tradescantia FRPR p R FDVR GH T. fluminensi VmR FRQVLGHUDGDV HVSpFLHV H[yWLFDV H LQYDVRUDV HP
3RUWXJDOSHORTXHQmRpSHUPLWLGDDVXDSODQWDomRHSURSDJDomR

)LJ32EVHUYDomR DR PLFURVFySLR yWLFR GH GLYHUVRV WLSRV GH SODVWRV FORURSODVWRV H OHXFRSODVWRV HP
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carota * H HP Pyracantha VS + $ LUULJDomR FRP VROXWR GH /XJRO UHYHOD D SUHVHQoD GH DPLGR QR
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) 2V FURPRSODVWRV ILEULODUHV GH Daucus carota DFXPXODP FDURWHQRV * HQTXDQWR RV FURPRSODVWRV
JOREXODUHV GH Pyracantha VS DFXPXODP [DQWRILODV + FXMD FRU DPDUHOD p UHYHODGD DSyV GHVFRORUDomR
GDVDQWRFLDQLQDVDFXPXODGDVQRYDF~ROR LQVHUomRQDHVTXHUGDVXSHULRUGDIRWR

11º Protocolo - Observação de Figuras de Mitose e Meiose e Determinação da Duração das

Diferentes Fases

2EVHUYDomRGHILJXUDVGHPLWRVHHPiSLFHVUDGLFXODUHVGHAllium cepa

D &RORTXHXPiSLFHUDGLFXODUGHAllium cepaQXPPLFURWXER (SSHQGRUI FRQWHQGRXPDJRWDGH +&O
1HJRWDVGHRUFHtQDDFpWLFD$JLWHPRGHUDGDPHQWHHGHL[HHPUHSRXVRGXUDQWH K
E 7UDQVILUDRPDWHULDOSDUDXPDOkPLQDOLPSDIDoDXPHVIUHJDoRHREVHUYH

$OWHUQDWLYDPHQWH
D &RORTXHXPiSLFHUDGLFXODUGHAllium cepaQXPDOkPLQDGHYLGURHVREUHHOHXPDJRWDGH +&O1
HJRWDVGHRUFHtQDDFpWLFD'HL[HHPUHSRXVRGXUDQWH PLQ
E $TXHoDVXDYHPHQWHDWpFRQFHQWUDURFRUDQWHHPWRUQRGRiSLFHVHPGHL[DUVHFDU
F 5HSLWDDRSHUDomRPDLVGXDVYH]HVDSyVDGLFLRQDUXPDJRWDGHRUFHtQDDFpWLFD
G 7UDQVILUDRPDWHULDOSDUDXPDOkPLQDOLPSDIDoDXPHVIUHJDoRHREVHUYH

2EVHUYDomRGHILJXUDVGHPLWRVHHPSUHSDUDo}HVGHILQLWLYDV

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GH FDGD XPD GDV IDVHV PLWyWLFDV FRQWDQGR R Q~PHUR GH FpOXODV HP FDGD XP GRV HVWiGLRV HP
YiULRVFDPSRVGHREVHUYDomRHXWLOL]DQGRDIyUPXODVHJXLQWH

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)LJ32EVHUYDomRDRPLFURVFySLRyWLFRGHGLYHUVDVIDVHVGHPLWRVHHPiSLFHUDGLFXODUGHAllium cepa
DSyVFRORUDomRFRPRUFHtQDDFpWLFD $ GHPHLRVHHPDQWHUDVGHAloeVSDSyVFRORUDomRFRPRUFHtQD
DFpWLFD % HGHPHLRVHHPDQWHUDVGHLilliumVSDSyVFRORUDomRFRPKHPDWR[LOLQD &'

12º Protocolo - Microscopia Eletrónica

3ULQFtSLRVGHIXQFLRQDPHQWRGR0LFURVFySLR(OHWUyQLFRGH7UDQVPLVVmR

3ULQFtSLRVGHIXQFLRQDPHQWRGR0LFURVFySLR(OHWUyQLFRGH9DUULPHQWR

)LJ35HSUHVHQWDomRHVTXHPiWLFDSDUDFRPSDUDomRGRVPHFDQLVPRVGHIXQFLRQDPHQWRGRPLFURVFySLR
HOHWUyQLFRGHWUDQVPLVVmRHGRPLFURVFySLRHOHWUyQLFRGHYDUULPHQWR

13º Protocolo - Observação de imagens de Microscopia Ótica e Eletrónica

'LVFXVVmR GH LPDJHQV GH 0LFURVFRSLD ÏWLFD H 0LFURVFRSLD (OHWUyQLFD GH 7UDQVPLVVmR H GH
9DUULPHQWR

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Guia Pratico Biologia Celular 4ed 2023

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Guia Pratico Biologia Celular 4ed 2023

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1º Protocolo - Microscopia Ótica

2º Protocolo - Microscopia Ótica (Continuação), Observação de Procariotas

3º Protocolo - Observação de Procariotas (Continuação), Observação de Algas

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11º Protocolo - Observação de Figuras de Mitose e Meiose e Determinação da Duração das

12º Protocolo - Microscopia Eletrónica

13º Protocolo - Observação de imagens de Microscopia Ótica e Eletrónica

Vermelho Sudão III

Reagente de Schiff (P.A.S.)

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