NANO-EMULSION

(NANO NH TNG)
TS. L TH HNG NHAN

I.1. H nh tng (emulsion)

I.1.1. Tng quan

Tnh cht v bn ca h nh tng

Tyndall effect

Classification

Particle size

Appearance

( m)

(Macro)
Emulsion

Thermo

Reflected

Scattering

dynamic

light

light

stability

Unstable

1 - 10

Opaque

No

No

0.1 - 1

Bluish

Weak
blue

Weak
red

Blue

Red

emulsion
Nano
emulsion

0.05 - 0.1

Translucency

Microemulsion
0.02 - 0.05

Stable or
unstable

Transparency
Transparency

Stable
Stable

No
- 0.02
Particle0.005sizes
and optical Noproperties

Solubilizasion

Source : Yukagaku, Abe, 41, p117(1992)

The Tyndall effect is light scattering by particles in a colloid or particles, in that the
intensity of the scattered light depends on the fourth power of the frequency,
so blue light is scattered more strongly than red light

Microemulsion:
Sc cng b mt ~ 0

Dch trong sut

nht thp

c im phn bit c bn gia microemulsion v cc

nh khc:
-Kch thc git
-Mc c
- bn ng hc

I.2. Phn loi

Gii thiu
Type of emulsion

Particle size

Macroemulsion (B) >400nm (0,4

Nanoemulsion(A)

100 400nm

Microemulsion(C)

<100nm

C
Particle size
>400nm

Appearance
White

100 400

Blue White

< 100

Translucent

< 50nm

Transparent

I.3. Nh Nano (nanoemulsion miniemulsion finely dispersed emulsion

sub-micronemulsion (SME)- utrafine emulsion)

H nh nano: kch thc phn t phn tn (droplet) t 100400nm (20 500nm), c mu hi xanh nht.
Tnh cht h nh nano ph thuc vo thnh phn pha, iu kin
cn bng nhit ng pha v phng php to h nh.
Phng php to h nh nano: s dng phng php ng
ha di p sut cao.

bn: thm cht HBM, nng khong 1-3%.

Ouzo effect

Pha phn tn t n c vai tr l cht HBM, phn tn

di kch thc nano trong pha lin tc to h nh
nano.

PH NG PHP TO H NANO -NH TNG

Nh ha
bng NL thp
(Low Energy)

Nh ha
t ng

Nh ha
bng nng
lng u
vo cao

Phase
Inversion
Temperature

Ultrasonicatio
n

Phase
Inversion
Composition

ng ha p
sut cao

Microfluidization

1.1. Tc ng ca sng siu m trong dung dch lng

Sng siu m l sng dc, c chiu di sng khong 10 -10-3cm

khng nng lng tng tc vo lin kt ha hc
Khi chiu x siu m trong mi trng lng li sinh ra nng
lng ln do hin tng vt l cavitation (hin tng kh thc):

Cht lng S co
gin
+
cht
ultrasonic lng

Negative
perssure
vt
bn ko
ca cht
lng

To l
hng
(bt)

Kch
thc
bt tng
dn

V
bt

Hot
spo
t

B. PH NG PHP TO H NANO -NH TNG

S tng dn kch thc dn n s b bt trong lng cht
lng di tc dng ca ultrasonic

Hotspot

Hot-spot c to ~ 5000oC, p ~1000atm, thi gian sng ngn

hn 1microsecond, tc gia nhit-lm lnh >10t oC/giy
(do trong mi trng xung quanh l lng lnh nn s gia nhit
nhanh chng c dp tt, nn n tn ti trong thi gian
ngn.)

Nh tng ha bng sng siu m

Cavitation l hin tng ch o trong qu trnh to nano nh

tng bng ultrasonic nh vo hot-spot. S ph v cc bt to ra
nhng sng cc mnh lan ta trong dung dch ph v cc phn
tn lng thnh cc vi ht hnh thnh nn h nh tng.

Mt dng my to ultrasonic

1.2. Nh tng ha bng sng siu m

C ch hnh thnh h nh tng bng siu m gm hai bc:

Bc u tin: sng siu m tc ng ca vo b mt phn
chia pha gia pha lin tc v pha phn tn, gip phn tn cc
git vo pha lin tc.
Bc th hai: dc tc ng ca hin tng cavitation to
nn cc hiu ng mnh m ca sng lm ph v cc git phn
tn lng thnh nhng vi git c kch thc rt nh. Hnh thnh
nn h nano nh tng

T hn hai thp k qua, cc nh nghin cu v sn xut

dc phm s dng h thng x l cao Microfluidizer
to cc dung dch phn tn ng nht vi kch thc ht phn
tn c nano.
Ban u, hai dung dch (thng l pha nc v pha du
mt l pha phn tn v mt l pha lin tc) c th c trn
vi nhau v c x l to ra dung dch nh tng th (tc
kch thc cc ht phn tn ln). Sau nh tng th s
c a vo h thng x l cao Microfluidizer tip tc x
l nhm thu c h nano-nh tng ng nht v n nh

Qu trnh x l trong Microfluidizer c m t theo s

sau:

Bm p sut cao
(1.500 - 40.000psi)
y sp vo bung
tng tc
(v>400m/s)

Sp va chm trong
cc vi knh cc
nh ph v
cc ht phn tn
thnh vi ht

Sp c lm lnh
h nano-nh
tng

ng ha p sut cao thc hin bng cch

cho dng phn tn i qua mt knh hoc l rt hp
di tc ng ca p sut rt cao nhm ph v cc
ht c kch thc ln thnh vi ht.

H thng ng ha p cao gm ba khu vc

chnh:
Bm p sut cao
Khoang ng ha
Khoang trao i nhit

Cu to chnh ca khoang ng ha:

Cu to chnh ca khoang ng ha:

Mt m
vt cnh

(a)
(b)
(c)
(a) Van (valve)
(b) Vng m (impact ring)
(c) Mt m c nh (seat)

Mt m
cong

ng ha p sut cao c thc hin theo cc bc nh sau:

Bm p sut cao
y dng sp vi
tc c cc ln
n khoang ng
ha.

- Lc xoy dng chy

cao v s chuyn ng
hn n
- S va chm mnh
vo van v vng m
ph v size ht

a sp ng ha
vo khoang trao
i nhit h to
chun b cho
cycle tip theo

Nh vy, t c hiu qu ng ha cao, ta thng xem xt

cc s liu sau:
p sut
Nhit
Thit k ca mt m (seat) v vng m
Lu lng
S cycle.
Cc my ng ha p cao c th t dung lng t 55
4.500 lt ty theo qui m.
Bm cao p dng mt piston chy bng mt trong mt
hnh tr bng thp c th nng p ln n 40.000 psi.
Qu trnh ny sinh ra lng nhit ng k nn cc piston
v xi-lanh phi c lm mt bng nc.

M phm

Y hc
Thc phm

Kem dng m

Sa sn chc da

Nh tng dng tc

Sa phc hi cho da

Nh tng lm trng da

Phn b nhiu trong c th ngi v c trong t nhin

L yu t kt hp (cofactor) ca t nht 3 enzyme ti ti th ca mi t bo to

ra ATP (adenosin triphosphat) cho nng lng

L cht chng oxy ha

The inner core

consisting of any
oil

Drug or nutrient
molecules within
the core

The emulsifier
layer surrounding
the nano-particle.

MicroFluidics Technology Fair

Thuc khng vi trng

(Antiambiral emulsion)

Phng nga bnh

(prophylatic in bioterrorism attack)

Cht ty u khng c hi
(non-toxic disinfectant
cleaners)

Vc-xin cht nhn

(mucosal vaccines)

K thut cy ghp t bo
(cell culture technology)

Prepared by Holly R-Fischer, MFA,

For NanoBio: 01/13/2009

ANTI-OXIDANT SYNERGY
(IN VIVO)

FORMATION

(ASF) STUDIES

Nude Mice
since they lack a fully-functional immune system
Injection of Cancer Cells
by subcutaneous injection of Mouse or Human Cancer
cells (300,000 cells /ml)
Injection of ASF solution
once tumors were detected
Measure the tumor diameter
quantified daily in accordance with the longest diameter

The neuroblastoma in the nude mice

Tumor

Control
(no treatment)

No Tumor

ASF
Nano-emulsion

Subcut Injection vs Topical Delivery of ASF containingnanoemulsions on neuroblastomainduced tumor growth rate

- 4%

- 65%

Subcut inj

Subcut inj

- 63%

Transdermal

Thc phm

Microemulsion versus Nanoemulsion of Soy Protein

Homogenized

Nanoemulsified

Nanoemulsions are stable dispersions which do not agglomerate

and which demonstrate antimicrobial activity

Gram + Staph Aureus Bacteria

Staph Aureus Control

Staph aureus streaked

empty nanoemulsion