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BF3513 GERNERAL FOOD TECHNOLOGY


Công nghệ thực phẩm đại cương Chapter 1. RAW FOOD MATERIALS PROPERTIES
WATER IN FOOD
Assoc. Prof. Dr. Chu Ky Sơn • Assoc.Prof.Dr. Luong Hong Nga
Assoc. Prof. Dr. Vu Thu Trang • School of Food Technology and Biotechnology
Assoc.Prof.Dr. Luong Hong Nga • Hanoi University of Science and Technology
School of Food Technology and Biotechnology • Email: luonghongnga@gmail.com
Hanoi University of Science and Technology
Email: luonghongnga@gmail.com

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WATER AND FOOD Water properties


Water in food
Moisture content
Water activity
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WATER IN FOOD
• All foods contain at least some water
• Free water
• Held inside cells
• Maintains properties of free water
• May be removed
• Bound water
(Physicochemical bound water: Hydrogen bond,
electrostatic bond
Chemically bound water: covalent bond )
• Is part of molecule structure
• Reduced mobility
• Does not retain properties of free water

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Food moisture
1. Wet basic (wb) ADSORPTION EQUILIBRIUM
2. Dry basic (db)

water 20 g The term adsorption


equilibrium refers to the
wb = 20% steady state condition that is
Dry
matter 80 g db = 25% ultimately reached when
adsorption and desorption
are going on at the same
time.

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KELVIN EQUATION
Surfaces with porous structure contain voids that promote transport of water by capillary adsorption.
Capillary adsorption occurs when voids in the cellular structure are of the size to hold water in liquid form by
forces of surface tension.

SORPTION
ISOTHERM

Sorption isotherms are graphical plots of the equilibriumbetween surface adhesion


forces and the partial pressure of the gaseous adsorbent at the boundary

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LANGMUIR MODEL

FREUNDLICH
MODEL

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BET MODEL

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OTHER MODELS

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WATER ACTIVITY AND SHELF LIFE OF FOOD

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aw mesurement CARBOHYDRATE- RICH RAW MATERIALS

Assoc.Prof.Dr. Luong Hong Nga


Dept. of Food Tech.
School of Food Tech. and Biotech.
Hanoi Uni. of Sci. and Tech.
h ttp ://www.fo o d re c a p .n e t/wp -c o n te n t/u p l o a d s /2 0 1 0 /0 8 /wa te r-a c ti v i ty -m e te r.j p g ĐT: 0383325179
Email: luonghongnga@gmail.com
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- Are the substances with molecular formation as


WHAT IS
Cx (H2 O) y which signifies molecules containing
Content CARBOHYDRATE
carbon atoms along with hydrogen and oxygen
1. Classification, atoms in the same ratio as they occur in water
2. Origin, seasons - Are polyhydroxy aldehydes or polyhydroxy
3. Chemical and functional properties and their effects on storage and ketones or substances that produce above
processing substances when hydrolyse
3.1. Starch - Contain chiral carbon
3.2. Sugar
3.3. Dextrin
3.4. Pectin
3.5. Oligossacharide
3.6. Others
4. Storage methods
5. Some typical carbonhydrate rich raw materials: Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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Roles to human being


• Energy supply
• Energy storage– glycogen
• Precusors substances (lipid, aminoacid)
• Constitute to cell structure and cell function
• Farmaceutical effects

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1. Monossacharides
2. Oligosacchardes
WHERE WE CAN 3. Polysaccharides
FIND ➢Starch
➢Cellulose
CARBOHYDRATE CLASSIFICATION ➢Pectin
➢Hydrocolloid
• Guar and locust bean gums
• Xanthan
• Carageenans
• Algins
• Gum arabic
➢Dietary fiber/ fiber

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ISOMERS

MONOSACCHARIDES
Can not be hydrolysed
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Class (DP)* Subgroup Components


Glucose, galactose,
Monosaccharides
fructose, xylose
Sugars (1–2) Sucrose, lactose, maltose, DẠNG VÍ DỤ VAI TRÒ
Disaccharides
trehalose Triose Glyceraldehyde, Chất trung gian của chu trình đường phân
Polyols Sorbitol, mannitol dihydroxyaetone Tiền chất của glycerol (tạo ra lipid)
Malto-oligosaccharides Maltodextrins Tetrose D-Erythrose Chất trung gian tổng hợp cacbohydrate (gluxit)
Oligossacharides (3–9) Raffinose, stachyose, Pentose D-ribose Thành phần cấu trúc của acid nucleic, RNA, co-enzyme
Other oligosaccharides
fructo-oligosaccharides Hexose D-glucose Đường chủ đạo của cơ thể
Amylose, amylopectin, D-fructose Chuyển hóa thành glucose và tiêu hóa trong cơ thể
Starch
modified starches D-galactose Tổng hợp trong tuyến sữa
Polyssaccharides (>9) Glycogen, cellulose, D-mannose Thành phần cấu tạo nên glycoprotein, glycolipid
Non-starch polysaccharides hemicellulose, pectins,
hydrocolloids.
* DP = Degree of polymerization
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OLIGOS ACCHARIDES
DIS ACCHARIDES

• Sucrose
• Oligo –few (1 vài) • Maltose
• Contain 2-10 monosaccharide
• Isomaltose
molecules
• Lactose
• Glycoside linkage

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SACCHAROSE

LACTOSE
(in Milk)
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LACTOSE INTOLERANCE

MALTOSE

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ISOMALTOSE
G l ucono-delta-lactone

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SORBITOL
GLYCEROL

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GLYCEMIC INDEX
SWEETNESS FACTORS

• GI ≤ 55: low.
• GI = 56- 69: medium.
• GI > 70: high

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POLYSACCHARIDES

• Number of monosssaccharides: still


controlversial
• > 10 monosaccharide→ polyme
• 10-15 monosaccharide → a little
• >20 monosaccharide → hay g ặp
• polysaccarit tự nhiên chứa 2 5 -7 5 g ố c
đường
• hầu hết p o ly saccarit chứa 8 0 -1 0 0 g ố c
đường , n h iều loại tới 3 0 0 0 g ố c (
xenluloza) Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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SOME POPULAR POLYSACCHARIDES


Gums
Tin h b ộ t

Pectin

Chitin

Xen lu lo za

Agar Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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POLYSSACHARIDES-RI CH RAW MATERIALS

• Sugar- rich Sugar-rich raw


• Starch-rich materials
• Fiber-rich
• Material for sugar production
• Pectin- rich
• Chitin-rich
• Agar-rich
• Gum-rich

• Vegetable and fruits


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Starchy food raw Fiber rich raw


materials materials
• Cereals
• Whole grain cereals
• Whole grain legume
• Starchy roots • Vegetables
• Nuts
• Roots
• Legumes, peanut, seasame

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Chitin rich raw


materials

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Carbohydrate properties and their effects on


food processing and storage

Agar

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Monosaccharide
properties • P roperties
• Solublize in water
• Isomerization
• Water absorption
• Form glycoside bonds
• Formation of a pyranose hemiacetal ring
Disaccharides • Crystalise
from
• Caramenlisation
• Interconversion
• Oxidation to aldonic acid and
aldonolactone
• Be oxidized
• Be reduced
• Non-enzyme browning

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Coversion in H +
C 12H22O11 + H2O →C 6H12O6 +
C 6H12O6
SUCROSE glucose fructose
• pH >6 → sản phẩm màu không
– Chemical mong muốn
properties Decomposed in alkaline

Caramenlization
L u o n g Ho n g Ng a As s o c .Pro f.Dr- Ha n o i Un i . o f Sc i . a n d
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Widely used as a food ingredient for many


purposes.
Unheated starch granule

A very wide selection of starches, both native

STARCH and modified (National Starch has >200


different starches for sale for selected
application)

Starch gelation and pasting characteristics


altered by other ingredients and by Heated starch granule
processing conditions

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Characteristic Amylose Amylopectin


Amylose
CHARACTERISTICS Form Essentially linear Branched
OF AMYLOSE AND
AMYLOPECTIN Linkage -1,4 (some -1,6) -1,4; -1,6

Polymer units 200-2,000 Up to 2,000,000

Molecular weight Generally <0.5 50-500 million


million
Gel formation Firm Non-gelling to
soft

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Amylopectin General Structure


Amylopectin

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Amy lopectin structure (Chaplin, 2004)


2.1 - Structure

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2 - Properties of Starch
2.1 - Structure
• The most common native starches are corn (maize),
rice, wheat, potato, tapioca (cassava) and waxy maize. Amylose Amylopectin
- Glucose units are linked in a linear way
• Except for waxy maize, these starches generally with α(1→4) glycosidic bonds - Glucose units are linked in a linear way
contain from 15-27% amylose. with α(1→4) glycosidic bonds.

Native • Waxy maize and other waxy native starches generally


contain less than 2% amylose.
-
-
Amylose is soluble in water
makes-up about 30% of the stored starch
in plants, though it varies depending on
- Branching takes place with α(1→6)
bonds occurring every 24 to 30 glucose

Starches
units
the source - It’s only soluble in hot water.
• High amylose starches contain more than 30% - Reacts with iodine => blue-black color
amylose and have quite different properties. They: - Reacts with iodine => blue-p urple
- The number of repeated glucose subunits color
• Are difficult to gelatinise > 100° C (n) is usually in the range of 300 to 3000, - Amylopectin is highly branched, being
• Can form films and fibres but can be many thousands formed of 2,000 to 200,000 glucose
units
Have more helical structure - may entrap fatty
acids – retards
granule swelling

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Starch gelatinizarion
PROPERTIES OF STARCH Starch granules → heat → water absorbing
→hydrating and swelling → breaking down
2.2 – Physical - S tarch becomes soluble in
hydrogen bonds and other bonds → diffuse →
w ater when heated.
Solubility gelatinization.
- Th e g ranules swell and
Gelatinized starch solution:
Water binding capacity / b u rst, the semi-crystalline
- Clarity
retention s tructure is lost and the
- Viscosity
s maller amylose molecules
Swelling Text - Consistency
s tart leaching out of the
- Volume increase
GelatinizationText granule, forming a network
th at holds water and
Starch granule (27 o C) Heat (40 o C) Swell (5 0 o C)
Viscosity Text i n creasing the mixture's
v i scosity.
- Th is process is called
Text
s tarch gelatinization
Gelatinize (70 -90 o C) Completely
Granule break down (65 o C)
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Starch gelation: Gelatinised starch solution →cooled down →


starch molecule rearranged (amylose) → 3-dimession network
called starch gel

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2 - Properties of Starch
STARCH 2.3 – Chemical
RETROGRADATION
Hydrolysis
- Hydrolysis of starches by acid (H+) or
enzymes.
- The enzymes that break down or
hydrolyze starch into the constituent
sugars are known as amylases…

D e x trinization
- If starch is subjected to dry heat, it
breaks down to form pyrodextrins(a
brown color), in a process known as
dextrinization.

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2.3 – Chemical
- The Iodine test is used to test for the
C h e mical tests presence of Starch.
- Iodine solution ( iodine dissolved in an
aqueous solution of potassium iodide) reacts
with starch producing a dark blue color.

- Starch indicator solution consisting of


water, starch and iodine is often used in
redox titrations: in the presence of an
IODINE COMPLEX oxidizing agent the solution turns blue,
in the presence of reducing agent the
blue color disappears because triiodide
(I3−) ions break up into three iodide
ions, disassembling the starch-iodine
complex…
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STARCH • Starch in food: vegetable


HYDROLYSIS 1%, roots, legume 10-20%,
cereals 70%
• Gelatinizing temp. 55-
80o C

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FILM FORMING ABILITY

NOODLE
PREPARATION
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Co-GEL WITH PROTEIN AND


OTHER POLYSSACHARIDE

PUFFING AB ILITY

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APPLICATION
IN FOOD
INDUSTRY CELLULOSE

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• Odourless, colourless
• Cellulose CELLULOSE • Beta glucoside linkage
• Fruits and vegetable: 1-3% (peel)
PROPERTIES • Hydrophilic properties
• Cereal: 1-3% • Can not dissolve in water
• Hdrolyse to glucose by acid or enzyme
• Nuts: 5-23,5%
• Linear molecules
• Roots: 1-3% • Contains lost of –OH → self assemble to form
microfibril structure→ chắc, dai
• Meat, fish, egg, milk: <0,7%
Australia department of health
http://apjcn.nhri.org.tw/server/info/books-
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phds/books/foodfacts/html/data/data2c.html

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DIETARY FIBER
CELLULOSE

• Support digestion: dietary fiber


• Support filtration
• Nutrition ??

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CELLULOSE IN FOOD
PECTIN
• Fiber supplement
• Thickening/ emulsifying agent
• Calorie reducer
• Anti-caking agent

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TYPE O F HM pectin.
WHAT IS PECTIN PECTIN • The most common type of pectin.
(BASED O N • Usually labeled as either “rapid -set” or “slow-set.” based on
time and temp. to set
APPLICATIO N) • Extracted from citrus fruit peels. Rapid-set pectin takes a
higher temperature and less time to set, while slow-set
pectin takes a lower temperature and more time.
• HM pectin needs sugar and very specific acid levels in order
to firm up → for fruit preserves, jams, and jellies.
• The dominant component • Rapid-set pectin → suspension → for jams and
of pectin is a linear chain preserves (suspension is essentially the fruit morsels
of galacturonic acid in that hang, suspended, in the viscous jam).
which a proportion of the • Slow-set pectin → do not for suspension → for smooth
carboxyl acid groups are jelly
present as methylesters

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TYPE OF PECTIN BASED ON WATER SOLUBILITY


LM pectin
2. Soluble Pectin (methoxyl po lygalacturonic): is a polysaccharide
consisted of acid galacturonic where some acid contains methoxyl
✓from citrus peels. group.
✓for low-calorie jams and jellies since it
relies on calcium instead of sugar to
solidify.
✓for dairy-based recipes that don’t need • Insoluble Pectin (protopectin): combination form between pectin
sugar, too. and araban (polysaccharide in cell wall).
✓LM pectin gets increasingly firmer as
calcium is added until it hits a saturation
point. At that time, the process reverses
and it becomes less firm.

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SOURCE OF PECTIN

ROLE OF PECTIN
• Important constituent on fruits
• Gelling agent
• Stablizers (For fruit opalescent or cloudy
juice)
• Thickening agent
• Can be hydrolyzed into smaller part
• Food fiber
• Structurizing agent
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PROPERTIES OF PECTIN • Thickening agent: Due to relatively low viscosities. However, pectin is
applied to drinks and fruit preparations because of its good mouthfeel and
• Gelling flavor release
• Thickening • Reactivity with protein: forming stable networks with protein→ stabilizer of
• React with protein acidified protein drinks and desserts in order to prevent protein coagulation,
and improver of various types of bread and dough in order to strengthen and
• Acid resistant stabilize network
• Acid resistant: pectin shows remarkable stability at acidic condition

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• Agar-agar is a dried, hydrophilic, colloidal


polysaccharide extracted from one of a
number of related species of red algae
(class Rhodophyceae)
AGAR IN FOOD
WHAT IS
AGAR
it contained galactose, 3,6-anhydro-galactose
and inorganic sulfate bonded to the carbohydrate

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GELATION
PPROPERTIES OF AGAR PROCESS
• Solubility??? • The gelling portion of agar-agar has
a double helical structure.
• Double helices aggregate to form a
three-dimensional structure
framework which holds the water
molecules within the interstices of
the framework. Thus, thermo-
reversible gels are formed.
• The gelling property of agar-agar is
due to the three equatorial hydrogen
atoms on the 3,6-anhydro-L-
galactose residues, which constrain
the molecule to form a helix. The
interaction of the helixes causes the
formation of the gel
Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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AGAR APPLICATION

BIOTECHNOLOGY (PLANT TISSUE CULTURE FRUIT JUICE DAIRY PRODUCTS JAM AND BAKERY
CULTURE MEDIA (MICROBILOGY) PHARMACEUTICALS

CRICKET FARMING
MEAT & HAM
SOFT CANDY DENTISTRY
Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . GELLING AGENT Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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CARRAGEENAN
• A family of linear sulphated polysaccharides (M~500-700) that are
extracted from red edible seaweeds.
CARRAGEENAN • Found in 1842-1862
• made up of repeating galactose units and 3,6 anhydrogalactose (3,6-
AG), both sulfated and nonsulfated. The units are joined by alternating
α-1,3 and β-1,4 glycosidic linkage
• Carrageenan consisted of D- Galactoze (17 – 31%), little L- Galactose.

Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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TYPE OF CARRAGEENAN
- Reduction in alkaline solution
❑ Kappa carra ge enan: polym er of D-ga lactose-4-sunlfate
- Precipitation
and 3,6 -anhydro D-galactose, consisted of 25% sulfate and - Swelling
34% 3,6-anhdro D-galactose linkage.
- Gelation
❑ Iota carrag e enan: Simil ar to Kappa- exc ept 3,6-anhydro PROPERTIES OF - Hydrolysis
D-galactose is sulf ated at C2. Consisted of 32 % sulfa te CARRAGEENAN - Viscosity
and 30% 3,6-anhdro D-galactose linkage. - Combine with protein
❑ Lambda c arrag e enan: most of monomers ar e D-g ala ctose- - Combine with starch
2-sunlfate (linkage at 1,3) and D-g al actose-2 ,6-disulfate . - Emulsion properties
Consisted of 35% sulf ate and without 3,6-anhdro D -
galactose linkage.


Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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Combine with protein

❑ There the linkage between cation in protein and sulfate group (bearin g negative charge) of
carrageenan . If thereis any metal anion → m0re linkage .
CHITIN

Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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CHITIN, CHITOS AN PROPERTIES


Chitin in Nature • White color/
• Odourless, tasteless
Exos keletons of arthropods Shells of mollus ks • Soluble in acid
• Molecular weight105 – 106 Da
• Deacetylation degree
• Viscosity
• Antimicroorganism
➢Link with Gram- microorganism → Lost of permeability
Spines of diatoms
➢Inhibitor of some enzyme
Cell walls of fungi, mold, yeas t
• Hydrolysis
Other invertebrate animals • Reaction by –OH
• Reaction by –NH2
• Oxidation
• Film forming Kurita, K. Controlled functionalization of the polysaccharide chitin.
Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch . Progress in Polymer Science, 2001, 26(9), 1921 -1971
Lu o n g Ho n g Nga Asso c.P ro f.Dr- Han o i Un i . o f Sci . an d Te ch .

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APPLICATION OF CHITOSAN
• Food additives

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